DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Net1

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_006254268.1	Gene:	Net1 / 307098	RGDID:	1305034	Length:	595	Species:	Rattus norvegicus

Alignment Length:	636	Identity:	124/636 - (19%)
Similarity:	223/636 - (35%)	Gaps:	175/636 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly  1334 PPPPTDP---------PVMSEEMITGL-----------------------IVPAPGWNGTSK--- 1363
            |..|..|         .|:||:...||                       :.|.|.|:.|.|
  Rat     5 PAAPKQPRPRRRSRRASVLSEDQAAGLPADTPGPAANERCSLRRGSSFTFLTPGPHWDFTLKRKR 69

  Fly  1364 -----DLYSPEA--ESSPATSFVDPAAMAAQLA--VGGVGGVVVGGLGVA-----------KPTS 1408
                 |:.|..:  ...|:...|.|......||  :..|....|...|..           .|.|
  Rat    70 REKDDDVVSLSSLDLKEPSNKRVRPLGRVTSLANLISPVRNGAVRRFGQTIQSFTLRGDHRSPAS 134

  Fly  1409 RTSSFEIENLLKTAEQET-RKSSPTGSVTSSVSTTALTPSRQLTDAEKLRKVVMELVDTERTYVK 1472
            ...||.    ..|....| |:||...|....:|......:|::    |.::.:.||...|:..::
  Rat   135 TQKSFS----RATVPTPTKRRSSALWSEMLDISMKESLTTREI----KRQEAIYELSRGEQDLIE 191

  Fly  1473 HLNNLLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFLQNLEESLDLEPDFNKFEHCGQFR 1537
            .|....:.|.:||.:.:.:|..|:..:||::...:......|..:.|:  .:||
  Rat   192 DLKLARKAYHDPMLKLSIMSEEELTHIFGDLDAYIPLHEDLLARIGEA--TKPD----------- 243

  Fly  1538 NVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPKQQHSSTLE--SY 1600
            ..:..||...:.::.....|..:|::...|:.:|...:.:..:|:|| .|..:...|..|:  |:
  Rat   244 GTVEQIGHILVNWLPGLNAYRGYCSNQLAAKALLDQKKQDPRVQDFL-QRCLESPFSRKLDLWSF 307

  Fly  1601 LIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDHLFR 1665
            |..|..|::||||||:::  |..|..|   ||...|  :||....|.|:         :.|...:
  Rat   308 LDIPRSRLVKYPLLLKEI--LRHTPKD---HLDVQL--LEKAILIIQEV---------LSDINLK 356

  Fly  1666 QHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKERLRQKKK 1730
            :.:..|:           ||     ::..::|.:.:|...:.|       :.|.||...|:.|
  Rat   357 KGESECQ-----------YY-----IDKLEYLDEKQKDPRIEA-------SKVLLCHGELKNK-- 396

  Fly  1731 LMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNS 1795
                    :.:::.|..:|.::.:|....|.                     :|.|.:||
  Rat   397 --------SGHKLYIFLFQDILVLTRPVTRN---------------------ERHSYQVY----- 427

  Fly  1796 TADFRNAFLKTIRQIIRESVRNMSIPMKNFGGSSGSVSGHSSQGMGSMGY------PGNSQTLER 1854
                |...  .:::::.|.:::..:.|   |||.....|:|.:.......      ||.|.||
  Rat   428 ----RQPI--PVQELVLEDLQDGDVRM---GGSFRGAFGNSDKAKNIFRVRFQDPSPGQSHTL-- 481

  Fly  1855 PKQQITIVHGSHTLGKPKKKSGSQRHSAGNIDYDNLSG-SQEADDLPPSVG 1904
              |...:.|........:......:.:|..::...|.. .:|.::..||.|
  Rat   482 --QANDVFHKQQWFNCIRSAIAPFQRAASPLELQGLPDLHEECEENNPSAG 530

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413
		PDZ_signaling	1221..1308	CDD:238492
		RhoGEF	1456..1647	CDD:238091	45/192 (23%)
		PH2_Tiam1_2	1650..1823	CDD:269957	22/172 (13%)
		PH	1693..1812	CDD:278594	17/118 (14%)
Net1	XP_006254268.1	RhoGEF	178..354	CDD:214619	48/205 (23%)
		PH_Net1	368..502	CDD:270044	31/189 (16%)
		PH	387..501	CDD:278594	27/162 (17%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910