DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Tiam1

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_038944357.1	Gene:	Tiam1 / 304109	RGDID:	1307455	Length:	1609	Species:	Rattus norvegicus

Alignment Length:	1616	Identity:	523/1616 - (32%)
Similarity:	767/1616 - (47%)	Gaps:	304/1616 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly   458 NNNTMGRASSQS--SRFSGSRSSHEIGRGYPPRNLYL--ELERERSCIEGSPPSDNVMFDNQCYA 518
            ::.|||.|.||:  ..|.|.:.: .:||.:..|:|.|  :..|.|....|.....|.....:..:
  Rat    16 DHKTMGNAESQNVDHEFYGEKHA-SLGRKHTSRSLRLSHKTRRTRHASSGKAIHRNSEVSTRSSS 79

  Fly   519 T------------TPSSSNGNSDQ--DQSYGQQQSSGQHPQQQQGPPQRSSRHQHHHQQAPNVTP 569
            |            .|.|..|..|.  |..:..:...|..|..........|........|.:|..
  Rat    80 TPSIPQSLAENGLEPFSQEGALDDFGDPIWVDRVDMGLRPVSYTDSSVTPSVDGSIVLTAASVQS 144

  Fly   570 TPGSPTSRL-------LLEYEMHLRNTLAKG---MDAESYSL-----------HTFEALLSQ-SM 612
            .|.|..|||       |.|.....|...:.|   |:..|:..           .:.|..||. |.
  Rat   145 MPDSEESRLYGDDAAYLAEGGRRQRPYTSNGPTFMETASFKKKRSKSADIWREDSLEFSLSDLSQ 209

  Fly   613 ENLEFAESLPGSNQRSPYPLRRPNAKSSTLPLPPHRPLSTIRDKERDRDRDGYYSDRNELIRERE 677
            |:|...|.:.||.:.......| ..::...|    |.|||.   :|.......|:.:|..::...
  Rat   210 EHLTSNEEILGSAEEKDCEEAR-GMETGASP----RQLSTC---QRANSLGDLYAQKNSGVKANG 266

  Fly   678 RERD------RGYLSD--------------HNSSFSN-----SRCASCIGESARAQWFRHSDGWR 717
            ..|:      |..:||              ...::||     .|.:.|:.|.|.          .
  Rat   267 GPRNRFSSYCRNLVSDIPDLAKHKMPPAAVETPTYSNYNTLPCRKSHCLSEGAT----------N 321

  Fly   718 SGSSTIGSGSGHGMMTQQIPGSGHKRSPWDSLPSLRQDSSLNDSGYKSARADS--LEQRAE---- 776
            ...|...|..|....|.|...:|             :.|...|||.:.|..|:  |.:|:.
  Rat   322 PQISLSKSMQGRRAKTTQDVNTG-------------EGSEFADSGIEGATTDTDLLSRRSNATNS 373

  Fly   777 ---------FIRQDSLRSEYLSDRESRYGIVQQASIESTDSRMCYLTSSEISDDDRMSLTTAVSD 832
                     |:..||..|.  :...:|.|:.:.           :....|:|..:..||..|.|.
  Rat   374 SYSPPTGRAFVGSDSGSSS--TGDAARQGVYEN-----------FRRELEMSTTNSESLEEAGSA 425

  Fly   833 EDDGES--VMASPYKAKATGTAASSFNCTGAVRKAGFLSVKKWLLRKKH-QIELARKRGWKGYWV 894
            ..|.:|  .::||.::....|||.     |.|||||.|:||.:|:.||: ::|.|.:|.||.|||
  Rat   426 HSDEQSSGTLSSPGQSDILLTAAQ-----GTVRKAGALAVKNFLVHKKNKKVESATRRKWKHYWV 485

  Fly   895 CLKGTTLLFYPCDSREGRSVEAAPKHLIIVDGAIMQPIPEHPKRDYIFCLSTAFGDAYLFQAPCQ 959
            .|||.||.||..|.|.|....:.|||.:.|:.:|:|.:|||||:|::||||.:.|||:|||...|
  Rat   486 SLKGCTLFFYESDGRSGIDHNSVPKHAVWVENSIVQAVPEHPKKDFVFCLSNSLGDAFLFQTTSQ 550

  Fly   960 VELENWVNSIHSACAAAFARHRGKTGTLHLLQEEIFRLEKAIESDHKLKHMAELQQSVVTDQETR 1024
            .|||||:.:||||||||.|||..|..||.||:.||.:||:.|:.|.|:|.|.|:|.|.|||.:.:
  Rat   551 TELENWITAIHSACAAAVARHHHKEDTLRLLKSEIKKLEQKIDMDEKMKKMGEMQLSSVTDAKKK 615

  Fly  1025 HQIQTQILQWEENLERLHCEQFRLRCYMASLQSGELPNPKSLLTHVSRPTKNTLNKLGVFTVSSF 1089
            ..|..||..||:|||:...:.||.|||:||||.|||||||.||...|||||..:.:||:|:||||
  Rat   616 KTILDQIFVWEQNLEQFQMDLFRFRCYLASLQGGELPNPKRLLGFASRPTKVAMGRLGIFSVSSF 680

  Fly  1090 HAFICARSPSLLNNLLAGRGATKRRPPMLSRSNSGSSRRS-------MQMNSRDEP--------- 1138
            ||.:.||:         |....:||...:||  |.|.|||       :...|:.:.
  Rat   681 HALVAART---------GEIGVRRRTQAMSR--SASKRRSRFSSLWGLDTTSKKKQGRPTINQVF 734

  Fly  1139 -----------EKTFKVAMPD------------------------------------NAYSTVYL 1156
                       |..|..::||                                    |..:...:
  Rat   735 GEGTDAVKRSLEGIFDDSVPDGKREKEVVLPSVHQHNPDCDIWVHEYFTPSWFCLPNNQPALTVV 799

  Fly  1157 RDAMSVEEFLASACARRNLNPMEHFVRVKKRRDMEDH-NYFVPHRNDLIENYLHNHEFVEVCMKI 1220
            |...:..:.|...|....|:...|::|:|..  ||:. .::||...:.|...|:..  :|:|.|:
  Rat   800 RPGDTARDTLELICKAHQLDHSAHYLRLKFL--MENKMQFYVPQPEEDIYELLYKE--IEICPKV 860

  Fly  1221 LYQVELQRT-TLEQMWGFSVEAELIENAERQDELCCYVSRVEDKSVAMHNGIIKGDEIMVINGAI 1284
            ...:.:::: .....:||     |:.:.:.......||:.|::..:|...|:..||||:.||...
  Rat   861 TQNIHIEKSDAAADNYGF-----LLSSVDEDGVRRLYVNSVKETGLASKKGLKAGDEILQINNRA 920

  Fly  1285 VSDLDMMYLESVLQEEQSLSMMMRSSRTEPPDLVGIMRVTDDMIDSLVCPPPPTDPPV-MSEEMI 1348
            ...|:...|:..| .:.||.:::   ||.|....|:     :::::   ||...|.|| :.|..:
  Rat   921 AGTLNSSMLKDFL-SQPSLGLLV---RTYPEPEGGV-----ELLEN---PPHRVDGPVDLGESPL 973

  Fly  1349 TGLIVPAPGWNGTSKDLYSPEAESSPATSFVDPAAMAAQLAVGGVGGVVVGGLGVAKPTSRTSSF 1413
            | .:...||.:.:|:.  ...||::|..               |.|            ....||.
  Rat   974 T-FLTSNPGHSLSSEQ--GSSAETAPEE---------------GEG------------PDLESSD 1008

  Fly  1414 EIENLLKTAEQET------RKSSPTGSVTSSVSTTA--LTPSRQLTDAEKLRKVVMELVDTERTY 1470
            |.::..|:.||.|      .:.||:.|..|....|:  |..:|||:||:|||||:.||::|||||
  Rat  1009 ETDHSSKSTEQVTAFCRSLHEMSPSDSSPSPQDATSPQLATTRQLSDADKLRKVICELLETERTY 1073

  Fly  1471 VKHLNNLLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFLQNLEESLDLEPDFNKFEHCGQ 1535
            ||.||.|:|.||:|:::||||:..|::.||||:.|:|.||.:||:.||:.:.|.||..|.|...|
  Rat  1074 VKDLNCLMERYLKPLQKETFLTQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQ 1138

  Fly  1536 FRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPKQQHSSTLESY 1600
            |:.|||::|.:||||.:.|||||:|||||:|..|||...:.:.|.:.||.|:||:||||||||||
  Rat  1139 FKKVLFSLGGSFLYYADRFKLYSAFCASHTKVPKVLVKAKTDTAFKAFLDAQNPRQQHSSTLESY 1203

  Fly  1601 LIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDHLFR 1665
            |||||||:|||||||:::..|||..::||.||..|:|.|.|||.||||||:||||:||:||.|..
  Rat  1204 LIKPIQRVLKYPLLLRELFALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIA 1268

  Fly  1666 QHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKERLRQKKK 1730
            :.....|:..|||.||||.:..|.|||....|||.||..||.|  ||||:|||.:.|:..:||||
  Rat  1269 EQTGEKKEVADLSMGDLLLHTSVIWLNPPASLGKWKKEPELAA--FVFKTAVVLVYKDGSKQKKK 1331

  Fly  1731 LMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNS 1795
            |:|....:...|.:..|::.:||...:||||..:.|.:::.:.|::|::|:.:.|.|:|:.|..|
  Rat  1332 LVGSHRLSIYEEWDPFRFRHMIPTEALQVRALPSADAEANAVCEIVHVKSESEGRPERVFHLCCS 1396

  Fly  1796 TADFRNAFLKTIRQIIRESVRNM-----SIPMKN----FGGSS-GSVSGHSSQGMGSMGYPGNSQ 1850
            :.:.|..|||::..|:|:..|..     |:|...    |||.. .::.|         ..|..|:
  Rat  1397 SPESRKDFLKSVHSILRDKHRRQLLKTESLPSAQQYVPFGGKRLCALKG---------ARPAMSR 1452

  Fly  1851 TLERPKQQITIVHGSHTLGKPKKKSGSQRHSAGNIDYDNLSGS--QEADDLPPSVG 1904
            .:..|         |.:||:.:::....|.:   ||.|.:|.|  ::....||..|
  Rat  1453 AVSAP---------SKSLGRRRRRLARNRFT---IDSDAISASSPEKEPQQPPGGG 1496

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	70/125 (56%)
		PH	873..974	CDD:278594	53/101 (52%)
		TIAM1_RBD	1141..1219	CDD:176413	19/114 (17%)
		PDZ_signaling	1221..1308	CDD:238492	19/87 (22%)
		RhoGEF	1456..1647	CDD:238091	110/190 (58%)
		PH2_Tiam1_2	1650..1823	CDD:269957	70/177 (40%)
		PH	1693..1812	CDD:278594	44/118 (37%)
Tiam1	XP_038944357.1	PH1_Tiam1_2	450..576	CDD:269937	70/125 (56%)
		Tiam_CC_Ex	590..687	CDD:408184	51/96 (53%)
		Ubiquitin_like_fold	784..850	CDD:421700	13/67 (19%)
		PDZ_signaling	864..943	CDD:238492	19/87 (22%)
		RhoGEF	1062..1251	CDD:214619	108/188 (57%)
		PH2_Tiam1_2	1253..1424	CDD:269957	68/172 (40%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	226	1.000	Domainoid score	I2435
eggNOG	1	0.900	-	-		E1_KOG3519
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	608	1.000	Inparanoid score	I891
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002962
OrthoInspector	1	1.000	-	-		otm45318
orthoMCL	1	0.900	-	-		OOG6_105244
Panther	1	1.100	-	-	O	PTHR46001
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X2782
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	12	11.820