DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Prex1

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_808450.2	Gene:	Prex1 / 277360	MGIID:	3040696	Length:	1650	Species:	Mus musculus

Alignment Length:	1445	Identity:	271/1445 - (18%)
Similarity:	486/1445 - (33%)	Gaps:	463/1445 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly  1384 MAAQLAVGGVGGVVVGGLGVAKPTSRTSSFEIENLLKTAEQETRKSSPTGSVTSSVSTTALTPSR 1448
            |.|..:.||.||...||.| |.|.:|                       |.| |.....|....|
Mouse     1 MEAPGSGGGDGGGDPGGDG-AHPDAR-----------------------GPV-SGPCAAARDSER 40

  Fly  1449 QLTDAEKLRKVVM-ELVDTERTYVKHLNNLLEHYLEPMKR------ETFLSNAEINALFGNIHEI 1506
            ||    :||..|: |::.|||.||..|..|...:|:.:::      |..|:...:..||.||.:|
Mouse    41 QL----RLRLCVLNEILGTERDYVGTLRFLQSAFLQRIRQNVADSVEKGLTEENVKVLFSNIEDI 101

  Fly  1507 VTFQRQFLQNLEESLDLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVL 1571
            :...:.||..||..|..||   :.:|         .:|:.||.:.:.|.:|..:|::|.||.::|
Mouse   102 LEVHKDFLAALEYCLHPEP---QSQH---------ELGNVFLKFKDKFCVYEEYCSNHEKALRLL 154

  Fly  1572 HPNEGNHALQEFLAA---RNPKQQHSSTLESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLC 1633
            .......|::.||.:   ...::.....||.||:.|||||.||||||:::...|..:..:|..:.
Mouse   155 VELNKVPAVRAFLLSCMLLGGRKTTDIPLEGYLLSPIQRICKYPLLLKELAKRTPGKHPDHTAVQ 219

  Fly  1634 EALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISDF-- 1696
            .||:.|:.|..:|||.:|..|:..|:     .|.|.              :..|.|..|::|.
Mouse   220 SALQAMKTVCSNINETKRQMEKLEAL-----EQLQS--------------HIEGWEGSNLTDICT 265

  Fly  1697 -------LGKIKKGLELHAMCFVFKSAVVFLCKERLR---QKKKLMGVSSKNATNEVEIIRYQVL 1751
                   |.||..|.......|:|.:.:|: ||.:.|   .||......|.|.:  :.|.|.::.
Mouse   266 ELLLQGNLLKISAGNIQERAFFLFDNLLVY-CKRKSRVTGSKKSTKRTKSINGS--LYIFRGRIN 327

  Fly  1752 IPVTEVQVRASSAKDMDSHFL-----WELIHLRSQLQRRSEKVYVLSNSTADFRNAFLKTIRQII 1811
            ..|.||:.......|..|:..     |: ||     .....|.:|....||:.:..:|..   :|
Mouse   328 TEVMEVENVEDGTADYHSNGYTVTNGWK-IH-----NTAKNKWFVCMAKTAEEKQKWLDA---LI 383

  Fly  1812 RESVRNMSIPMKNFGGSSGSVSGHSSQGMGSMGYPGNSQTLER-----PKQQITIVHGSHTLGKP 1871
            ||..:..|:.:                ||....|...::..|:     ..:::.::.     .:.
Mouse   384 REREQRESLKL----------------GMERDAYVMIAEKGEKLYHMMMSKKVNLIK-----DRR 427

  Fly  1872 KKKSGSQRHSAGN------IDYDNLSGSQEADDLPPSV---GVVHYASGHTHGQQMQPAGFRGRS 1927
            :|.|...:...||      ::...:|.::|..:|..::   |::|:.|
Mouse   428 RKLSTVPKCFLGNEFVAWLLEIGEISKTEEGVNLGQALLENGIIHHVS----------------- 475

  Fly  1928 KTVGDVTEITCSSPEPHQQQQQQ--------------QQQQQQLMQQGHAHAHPHPHPHPREPPP 1978
                          :.||.:.:|              :.:.:.:|.:|   ...:...|....|.
Mouse   476 --------------DKHQFKNEQVMYRFRYDDGTYKARSELEDIMSKG---VRLYCRLHSLYAPV 523

  Fly  1979 PPIRQPHLHHHSSDIERIDPGTK------SEGEEDSQQGTIRPKATLGRTPNHLTLSTTSTLSVG 2037
            ...|..||..:.|    :.||:|      ::|:..:::..:                   .|.||
Mouse   524 IKDRDYHLKTYKS----VVPGSKLVDWLLAQGDCQTREEAV-------------------ALGVG 565

  Fly  2038 --STGSQARLIQSS-----------HPPASYQPVLMKD-------------LGK-----PSPDES 2071
              :.|....:::.|           |.....:....|:             |.|     |..|:.
Mouse   566 LCNNGFMHHVLEKSEFKDESQYFRFHADEEMEGTSSKNKQLRNDFKLVENILAKRLLIPPQEDDY 630

  Fly  2072 ELESESEVHAAQVAASFNLSLGSSGSLFASDARDNQTQSRTQSQIHSLHPSPRQSPRQSPRQSPK 2136
            ..:.|.:..|..|.:....||.....|.|.            .:|:|::
Mouse   631 GFDLEEKNKAVVVKSVQRGSLAEMAGLQAG------------RKIYSIN---------------- 667

  Fly  2137 QDIEVIEIFESEAERL----------------AASQQVAVVHQHP---SDQARESGHKQRRDRDR 2182
            :|:..:..| ||.|.:                ..:::...|..||   |.|.|.:.
Mouse   668 EDLVFLRPF-SEVETILNQFFCSRRPLRLLVATKAKETIKVPDHPEALSFQIRGTA--------- 722

  Fly  2183 NPTETDATARFMDKHLSDL------EQENLT----DGTCDIVAYMESL---REKAF--------- 2225
             |....|..|..:...:.|      .:.|.|    ||..:::.:.::.   ||:|.
Mouse   723 -PPCVFAVGRGSEAVAAGLCAGQCILKVNGTSVANDGALEVLEHFQAFRNHREEALGLYQWVYHS 786

  Fly  2226 -DPQDLA--------EDTGDHQQNDSS---LSPEPQTIVENTQQTVVVDIESPSRPATESDSTGN 2278
             :...||        ||..:..|.||:   ||..||..:......|.:.:::
Mouse   787 HEDAQLARASQGAPDEDPQEDDQPDSALPLLSLGPQLSLHEDSAVVSLTLDN------------- 838

  Fly  2279 TTGPSERSGDETEEEATTSSLASHIVVIDKKVDKIFKAKSPERPASTKVKGGRRQIYISPDRCKS 2343
                .........|..:|:....|:      ::||.:.:...|.|:..::.......|....|.
Mouse   839 ----VHLEHGVVYEYMSTAGAKCHV------LEKIVEPRGCFRLAAKILEAFAVDDSIFVQNCG- 892

  Fly  2344 QGSSPVRIIKIKSPRTSLSDQGKRLSVCSSR-DNSQDRLSAGRRTPSPRRSSEGGGILKHTTGPD 2407
                  |::.:.|...::|.. :..::|.:: ::...|::..:...:..:|.
Mouse   893 ------RLMAMSSAIVTMSHY-EFHNICDTKLESIGQRIACYQEFAAQLKSR------------- 937

  Fly  2408 PGILKPPSSPAKSKSPDRSCLKKGGPS--HVCSVETLSPRVSP------------RGSMDHLSPD 2458
               :.||...| |..|...|.....|:  ||..:|...|:.:|            :.|:..|.|:
Mouse   938 ---VSPPFKQA-SLEPHPLCGLDFCPTNCHVNLMEVSYPKTTPSVGRSFSIRFGRKPSLIGLDPE 998

  Fly  2459 RGMTSSSCLRH------------SPRSSFDSHGESDHNLDVPHGSRKRSMSAHGSFETGTRPRAQ 2511
            :|:...:..:|            ||....||.|:..::            |::|| .:|...:..
Mouse   999 QGLNPMAYTQHCITTMAAPSWKCSPAVDEDSQGQGLND------------SSYGS-ASGAPSQQD 1050

  Fly  2512 ETYQYYPVYDNQTCSDHY--------VAAAPTGRYGGGTSSRSRLSKSLERSTSRDSTTTSSSYG 2568
            ....:....:::...|.|        ||.....:|   .:..:||..::...||.
Mouse  1051 RGLSFLLKQEDREIQDAYLQLFTKLDVALKEMKQY---VTQINRLLSTITEPTSA---------- 1102

  Fly  2569 HRAYGVSPDRNYVVFSSGPLRSQSAENTFSSQPIYD--PLPRHPPQPLHQPPMHHQQQQQQQQQQ 2631
                           :..|......|.|.||.|:.:  .:.|.....:.:......:.:|:....
Mouse  1103 ---------------APAPCDPSLVEETSSSPPVSEESEVDRTDHSGIKKVCFKVSEDEQEDSGH 1152

  Fly  2632 QYMSYAPEMGGYTGC 2646
            ..|||   ...|:.|
Mouse  1153 DTMSY---RDSYSEC 1164

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413
		PDZ_signaling	1221..1308	CDD:238492
		RhoGEF	1456..1647	CDD:238091	61/200 (31%)
		PH2_Tiam1_2	1650..1823	CDD:269957	42/189 (22%)
		PH	1693..1812	CDD:278594	30/135 (22%)
Prex1	NP_808450.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..33		15/56 (27%)
		RhoGEF	48..234	CDD:279015	60/197 (30%)
		PH_Collybistin_ASEF	239..391	CDD:269931	40/182 (22%)
		PH	267..384	CDD:278594	29/128 (23%)
		DEP	409..489	CDD:295306	14/115 (12%)
		DEP_2_P-Rex	501..593	CDD:239887	18/117 (15%)
		PDZ	630..697	CDD:214570	14/95 (15%)
		PDZ	714..768	CDD:238080	11/63 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	793..813		5/19 (26%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1022..1047		9/37 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1099..1129		8/54 (15%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3519
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1291
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.840