DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and TIAM2

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_001371475.1	Gene:	TIAM2 / 26230	HGNCID:	11806	Length:	1701	Species:	Homo sapiens

Alignment Length:	1796	Identity:	528/1796 - (29%)
Similarity:	806/1796 - (44%)	Gaps:	394/1796 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly   482 GRGYPPRNLYLELERERSCIEGSPPSDNVMFDNQCYATTPSSSNGNSDQDQSYGQQQSSGQHPQQ 546
            |.||..|:|      .|||:       :....||.||:..........:..:|...:::......
Human    52 GAGYKSRSL------ARSCL-------SHFKSNQPYASRLGGPTCKVSRGVAYSTHRTNAPGKDF 103

  Fly   547 QQGPPQRSSRHQHH---HQQAPNVTPTPGSPTSRLLLEYEMHLRNTLAK---------GMDAES- 598
            |......|:.:..|   |:.|.|      ..|||   :...||.|...:         |.|.:|
Human   104 QGISAAFSTENGFHSVGHELADN------HITSR---DCNGHLLNCYGRNESIASTPPGEDRKSP 159

  Fly   599 ----YSLHTFEALLSQSMENLEFAESLPGSNQRSPYPLRR--PNAKSSTLPLPPHRPLSTIRDKE 657
                .:|...:..|.....|......:|..:...|..|.|  |...|.|.|:|
Human   160 RVLIKTLGKLDGCLRVEFHNGGNPSKVPAEDCSEPVQLLRYSPTLASETSPVP------------ 212

  Fly   658 RDRDRDGYYSDRNELIRERERERDRGYLSDHNSSFSNSRCASCIGESARAQWFRHSDGWRSG--- 719
                             |..|......|..|..|.::||..|..|.|..::...:...|.:.
Human   213 -----------------EARRGSSADSLPSHRPSPTDSRLRSSKGSSLSSESSWYDSPWGNAGEL 260

  Fly   720 SSTIGSGSGHGMMTQQIPGS---GHKRSPWD---SLPSLRQ---DS---SLNDSG------YKSA 766
            |...||....||....:..|   |..:.|::   ||.|||:   |:   ||:.||      |...
Human   261 SEAEGSFLAPGMPDPSLHASFPPGDAKKPFNQSSSLSSLRELYKDANLGSLSPSGIRLSDEYMGT 325

  Fly   767 RADSLEQRAEF-----------------------------------IRQDSLR------------ 784
            .| ||..|..|                                   .::|||:
Human   326 HA-SLSNRVSFASDIDVPSRVAHGDPIQYSSFTLPCRKPKAFVEDTAKKDSLKARMRRISDWTGS 389

  Fly   785 ----------------SEYLSDRESRYGIVQQASIESTDSRMCYLTSSEISD--DDRMSLTTAVS 831
                            |:|...|..  |:  ...::.:.....:|.|...:.  ..|...|.|:.
Human   390 LSRKKRKLQEPRSKEGSDYFDSRSD--GL--NTDVQGSSQASAFLWSGGSTQILSQRSESTHAIG 450

  Fly   832 DE------------------DDGESVMASPYKAKATGTAASSFNC--------TGAVRKAGFLSV 870
            .:                  .:.|::..|...|:::..:.||...        .|.|||||:|..
Human   451 SDPLRQNIYENFMRELEMSRTNTENIETSTETAESSSESLSSLEQLDLLFEKEQGVVRKAGWLFF 515

  Fly   871 KKWL-LRKKHQIELARKRGWKGYWVCLKGTTLLFYPCDSREGRSVEAAPKHLIIVDGAIMQPIPE 934
            |..: ::|:.::||..:|.||.|||.|||.|||||....:......:||:..:..:.:|:|.:||
Human   516 KPLVTVQKERKLELVARRKWKQYWVTLKGCTLLFYETYGKNSMDQSSAPRCALFAEDSIVQSVPE 580

  Fly   935 HPKRDYIFCLSTAFGDAYLFQAPCQVELENWVNSIHSACAAAFARHRGKTGTLHLLQEEIFRLEK 999
            |||::.:||||.:|||.|||||..|.:|||||.::|||||:.||:..||..||.||:.:...|.:
Human   581 HPKKENVFCLSNSFGDVYLFQATSQTDLENWVTAVHSACASLFAKKHGKEDTLRLLKNQTKNLLQ 645

  Fly  1000 AIESDHKLKHMAELQQSVVTDQETRHQIQTQILQWEENLERLHCEQFRLRCYMASLQSGELPNPK 1064
            .|:.|.|:|.|||||.|||:|.:.|..|:.||.|||:|||:.|.:.||:|||:||||.|||||||
Human   646 KIDMDSKMKKMAELQLSVVSDPKNRKAIENQIQQWEQNLEKFHMDLFRMRCYLASLQGGELPNPK 710

  Fly  1065 SLLTHVSRPTKNTLNKLGVFTVSSFHAFICARSPSLLN-----------------------NLLA 1106
            |||...|||:|..|.:||:.:||||||.:|:|..|.|.                       :.||
Human   711 SLLAAASRPSKLALGRLGILSVSSFHALVCSRDDSALRKRTLSLTQRGRNKKGIFSSLKGLDTLA 775

  Fly  1107 GRGATKRRPPMLSRSNS-----GSSRRSMQMNSRDEPEKTFKVAMPDNAYSTVYLRDAMSVEEFL 1166
            .:|..||  |.:::.:.     ||:...:..::..|.: |: |...||...||.::....||:.|
Human   776 RKGKEKR--PSITQVDELLHIYGSTVDGVPRDNAWEIQ-TY-VHFQDNHGVTVGIKPEHRVEDIL 836

  Fly  1167 ASACARRNLNPMEHFVRVKKRRDMEDHNYFVPHRNDLIENYLHNHEFVEVCMKILYQVELQRTTL 1231
            ..||..|.|.|..:.::::|..| ::..|.:|...:.::..:::.  :||....:|.|:|.:|..
Human   837 TLACKMRQLEPSHYGLQLRKLVD-DNVEYCIPAPYEYMQQQVYDE--IEVFPLNVYDVQLTKTGS 898

  Fly  1232 EQMWGFSVEAELIENAERQDELCCYVSRVEDKSVAMHNGIIKGDEIMVINGAIVSDLDMMYLESV 1296
            ...:||:|.|::   .|||.....::|.|....:|...|:.||:|||.:||..|||||:..:|::
Human   899 VCDFGFAVTAQV---DERQHLSRIFISDVLPDGLAYGEGLRKGNEIMTLNGEAVSDLDLKQMEAL 960

  Fly  1297 LQEEQSLSMMMRSSRTEPPDLVGIMRVTDDMIDSLVCPPPPTDPPVMSEEMITGLIVPAPGWNGT 1361
            ..|:.                ||:         :|:..||.|...:.:            .|:.:
Human   961 FSEKS----------------VGL---------TLIARPPDTKATLCT------------SWSDS 988

  Fly  1362 ------SKDLYSPEAESSPATSFVDPAAMAAQLAVGGVGGVVVGGLGVAKPTSRTSSFEIENLLK 1420
                  .|.|..|..:|.....|:|............|..:..|     ...|:|..
Human   989 DLFSRDQKSLLPPPNQSQLLEEFLDNFKKNTANDFSNVPDITTG-----LKRSQTDG-------- 1040

  Fly  1421 TAEQETRKSSPTGSVTSSVSTTALTPS------------------------RQLTDAEKLRKVVM 1461
            |.:|.:.:.....:..|:...|||..|                        |.|:||::||||:.
Human  1041 TLDQVSHREKMEQTFRSAEQITALCRSFNDSQANGMEGPRENQDPPPRSLARHLSDADRLRKVIQ 1105

  Fly  1462 ELVDTERTYVKHLNNLLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFLQNLEESLDLEPD 1526
            ||||||::|||.|:.|.|.||||::.||||:..|:.:|||::.|::.||:.||:.||:.:....|
Human  1106 ELVDTEKSYVKDLSCLFELYLEPLQNETFLTQDEMESLFGSLPEMLEFQKVFLETLEDGISASSD 1170

  Fly  1527 FNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPKQ 1591
            ||..|...|||.:||::|.:||||.:||||||.|||:|.|.||||...:.:.|.:.||.||||.:
Human  1171 FNTLETPSQFRKLLFSLGGSFLYYADHFKLYSGFCANHIKVQKVLERAKTDKAFKAFLDARNPTK 1235

  Fly  1592 QHSSTLESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQRIHEEY 1656
            |||||||||||||:||:|||||||:::.:|||..::||.||.||||.|||||.||||||:|:|:|
Human  1236 QHSSTLESYLIKPVQRVLKYPLLLKELVSLTDQESEEHYHLTEALKAMEKVASHINEMQKIYEDY 1300

  Fly  1657 GAIFDHLFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAVVFLC 1721
            |.:||.|..:...:.|:..:||.|:||.:..|.|||....|||.:|.|||  ..||||.||:.:.
Human  1301 GTVFDQLVAEQSGTEKEVTELSMGELLMHSTVSWLNPFLSLGKARKDLEL--TVFVFKRAVILVY 1363

  Fly  1722 KERLRQKKKLMGVSSKNATNEVEI--IRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQR 1784
            ||..:.||||.. :|:.|.|..::  .:::.|||::.:|||..:....:::.:|||||.:|:::.
Human  1364 KENCKLKKKLPS-NSRPAHNSTDLDPFKFRWLIPISALQVRLGNPAGTENNSIWELIHTKSEIEG 1427

  Fly  1785 RSEKVYVLSNSTADFRNAFLKTIRQIIRESVR--------------NMSIPMKNFGGSSGSVSGH 1835
            |.|.::.|..|.::.:...:|.||.|:||:.|              :..:|:||....|..::
Human  1428 RPETIFQLCCSDSESKTNIVKVIRSILRENFRRHIKCELPLEKTCKDRLVPLKNRVPVSAKLA-- 1490

  Fly  1836 SSQGMGSM---------GYPGNSQTLERPKQQITIVHGSHTLGKPKKKSGSQRHSAGNIDYDNLS 1891
            ||:.:..:         |..|....|:..:..::  .|:.:.|.|                 ...
Human  1491 SSRSLKVLKNSSSNEWTGETGKGTLLDSDEGSLS--SGTQSSGCP-----------------TAE 1536

  Fly  1892 GSQEADDLPPSVGVVHYASGHTHGQQMQPAGFRGRSKTVGDVTEITCSSPEPHQQQQQQQQQQQQ 1956
            |.|::....|             |:...| |....:..:...::|.....:.|:|..:|....:.
Human  1537 GRQDSKSTSP-------------GKYPHP-GLADFADNLIKESDILSDEDDDHRQTVKQGSPTKD 1587

  Fly  1957 L---MQQGHAHAHPHPHPHPREPPPPPI-------RQPHL-HHHSSDIERIDPGTKSEGEEDSQQ 2010
            :   .|:......|..||...:.|.|..       .||.| ..|...|:|....||.:      :
Human  1588 IEIQFQRLRISEDPDVHPEAEQQPGPESGEGQKGGEQPKLVRGHFCPIKRKANSTKRD------R 1646

  Fly  2011 GTIRPKATLGRTPNHLTL---STTSTLSVGSTGSQARLIQS 2048
            ||:. ||.:    .|.:|   |..:|:.:.|...:...:||
Human  1647 GTLL-KAQI----RHQSLDSQSENATIDLNSVLEREFSVQS 1682

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	61/125 (49%)
		PH	873..974	CDD:278594	47/101 (47%)
		TIAM1_RBD	1141..1219	CDD:176413	20/77 (26%)
		PDZ_signaling	1221..1308	CDD:238492	29/86 (34%)
		RhoGEF	1456..1647	CDD:238091	110/190 (58%)
		PH2_Tiam1_2	1650..1823	CDD:269957	64/188 (34%)
		PH	1693..1812	CDD:278594	42/120 (35%)
TIAM2	NP_001371475.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..21
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	201..250		15/77 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	265..293		7/27 (26%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	389..417		4/31 (13%)
		PH1_Tiam1_2	505..631	CDD:269937	61/125 (49%)
		Tiam_CC_Ex	645..742	CDD:408184	57/96 (59%)
		Ubiquitin_like_fold	811..881	CDD:421700	18/74 (24%)
		PDZ_signaling	888..973	CDD:238492	32/112 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1070..1092		0/21 (0%)
		RhoGEF	1103..1292	CDD:214619	108/188 (57%)
		PH2_Tiam1_2	1294..1466	CDD:269957	63/174 (36%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1500..1556		12/88 (14%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1568..1628		11/59 (19%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	228	1.000	Domainoid score	I2494
eggNOG	1	0.900	-	-		E1_KOG3519
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	689	1.000	Inparanoid score	I699
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002962
OrthoInspector	1	1.000	-	-		otm41178
orthoMCL	1	0.900	-	-		OOG6_105244
Panther	1	1.100	-	-	O	PTHR46001
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X2782
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	11	10.820