DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment sif and PLEKHG4

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_001123199.1	Gene:	PLEKHG4 / 25894	HGNCID:	24501	Length:	1191	Species:	Homo sapiens

Alignment Length:	963	Identity:	189/963 - (19%)
Similarity:	317/963 - (32%)	Gaps:	294/963 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly  1053 ASLQSGELPNPKSLLTHVSRPTKNTLNKLGVFTVSSFHAFI--CARSPSLLNNLLAG----RGA- 1110
            :.||..:||: :|||||:  ||......||........|::  ..|..:||.|..|.    :||
Human   294 SGLQLEQLPS-QSLLTHI--PTAGLPTSLGGGLPYCHQAWLDFRRRLEALLQNCQAACALLQGAI 355

  Fly  1111 --TKRRPPMLSRSNSG------------------------SSRRSMQMNSRDEPEKT----FKVA 1145
              .|..|..:.....|                        ...|.:....::.||.|    ::.|
Human   356 ESVKAVPQPMEPGEVGQLLQQTEVLMQQVLDSPWLAWLQCQGGRELTWLKQEVPEVTLSPDYRTA 420

  Fly  1146 M--PDNAYSTVYLRDAMSVEEFLASACARRNLNPMEHFVRVKKRRDMEDHNYFVPHRNDLIENYL 1208
            |  .|..|..|   |.:..:..|.|   .:.:..:| .|:..:.|:...|.         ||.:|
Human   421 MDKADELYDRV---DGLLHQLTLQS---NQRIQALE-LVQTLEARESGLHQ---------IEVWL 469

  Fly  1209 HN------HEFVEVCMKILYQV-----ELQRTTLEQM------------WG-----------FSV 1239
            ..      .|..|..:.:|.|.     ||.:...||:            |.           .::
Human   470 QQVGWPALEEAGEPSLDMLLQAQGSFQELYQVAQEQVRQGEKFLQPLTGWEAAELDPPGARFLAL 534

  Fly  1240 EAELIENAERQDELCCYVSRVEDKSVAMHNGIIKGDEIMVINGAIVSDLDMMYLESVLQEEQSLS 1304
            .|:|.|.:....:.|..::..|.........:...:|    ...::::|:......|||:
Human   535 RAQLTEFSRALAQRCQRLADAERLFQLFREALTWAEE----GQRVLAELEQERPGVVLQQ----- 590

  Fly  1305 MMMRSSRTEPPDLVGIMRVTDDMIDSLVCPPPPTDPPVMSEEM---ITGLIVPAP------GWNG 1360
              ::...|..|||                      ||....:|   .|||...|.      .| .
Human   591 --LQLHWTRHPDL----------------------PPAHFRKMWALATGLGSEAIRQECRWAW-A 630

  Fly  1361 TSKDLYSPEAESSPATSFVDPAAMAAQLAVGGVGGVVVGGLGVAKPTSRTSSFEIENLLKTAEQE 1425
            ..:|.:....:...|:..:.|....|.|.|..|...     ....|..:..||: .||.::..:.
Human   631 RCQDTWLALDQKLEASLKLPPVGSTASLCVSQVPAA-----PAHPPLRKAYSFD-RNLGQSLSEP 689

  Fly  1426 T------------RKSSPTGSVTSSVSTTALTP-SRQLTDAEKLRKVVMELVDTERTYVKHLNNL 1477
            .            |:....|......|.|...| |.......:|:.|:.|:|.|||.||:.|...
Human   690 ACHCHHAATIAACRRPEAGGGALPQASPTVPPPGSSDPRSLNRLQLVLAEMVATEREYVRALEYT 754

  Fly  1478 LEHYLEPMKRETFLS--NAEINALFGNIHEIVTFQ-RQFLQNLEESLDLEPDFNKFEHCGQFRNV 1539
            :|:|...:.|.....  ..:...||||:.::..|. ..||:.||......|
Human   755 MENYFPELDRPDVPQGLRGQRAHLFGNLEKLRDFHCHFFLRELEACTRHPP-------------- 805

  Fly  1540 LFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHP------NEGNHALQEFLAARNPKQQHSSTLE 1598
              .:..|||.:...|.:|:.:..:..::..::..      .:...||.:.|           .|.
Human   806 --RVAYAFLRHRVQFGMYALYSKNKPRSDALMSSYGHTFFKDKQQALGDHL-----------DLA 857

  Fly  1599 SYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDHL 1663
            |||:|||||:.||.||||:              |..|..|..:....:.|.|.:        .|.
Human   858 SYLLKPIQRMGKYALLLQE--------------LARACGGPTQELSALREAQSL--------VHF 900

  Fly  1664 FRQHQKSCKQPIDLSPGDLLYYGGVEW--LNISDFLGKIKKGLELHAMCFVFKSA------VVFL 1720
            ..:|           ..|||....::.  :|:.: .|::.:..|     ||.::.      .:||
Human   901 QLRH-----------GNDLLAMDAIQGCDVNLKE-QGQLVRQDE-----FVVRTGRHKSVRRIFL 948

  Fly  1721 CKERLRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSAKDM-------DSHFLWELIHL 1778
            .:|.|...|...|.:.      |:...|:          |:....|:       :|:..:|:...
Human   949 FEELLLFSKPRHGPTG------VDTFAYK----------RSFKMADLGLTECCGNSNLRFEIWFR 997

  Fly  1779 RSQLQRRSEKVYVLSNSTADFRNAFLKTIRQII-RESVRNMSIPMKNFGGSSGSVSGHSSQGMGS 1842
            |    |::...:||..|:...:.|:...|..:: |::|.|..:.|...          .|.|:|:
Human   998 R----RKARDTFVLQASSLAIKQAWTADISHLLWRQAVHNKEVRMAEM----------VSMGVGN 1048

  Fly  1843 MGY----PG----NSQTLE--------RPKQQITIV---HGSHTLGKPKKKSG 1876
            ..:    |.    |.:|:.        |.:..|.:.   |.|..||......|
Human  1049 KAFRDIAPSEEAINDRTVNYVLKCREVRSRASIAVAPFDHDSLYLGASNSLPG 1101

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413	18/89 (20%)
		PDZ_signaling	1221..1308	CDD:238492	17/114 (15%)
		RhoGEF	1456..1647	CDD:238091	49/199 (25%)
		PH2_Tiam1_2	1650..1823	CDD:269957	33/188 (18%)
		PH	1693..1812	CDD:278594	23/132 (17%)
PLEKHG4	NP_001123199.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..152
		SPEC	447..>598	CDD:321951	27/171 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	707..728		5/20 (25%)
		RhoGEF	736..906	CDD:306973	52/229 (23%)
		PH_puratrophin-1	899..1034	CDD:270062	31/171 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1150..1176
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.910