DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and scd1

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_594221.1	Gene:	scd1 / 2542337	PomBaseID:	SPAC16E8.09	Length:	872	Species:	Schizosaccharomyces pombe

Alignment Length:	673	Identity:	145/673 - (21%)
Similarity:	260/673 - (38%)	Gaps:	143/673 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly  1413 FEIENLLK---TAEQETRKSSPTGSVTSSVSTTALTPSRQLTDAEKLRKVVMELVDTERTYVKHL 1474
            :|:.|..|   |....|..:.|||::.|.:::.              |:|..||.:||..|::.|
pombe   197 YEVSNTTKSSSTPSPSTDDNVPTGTLNSLIASG--------------RRVTAELYETELKYIQDL 247

  Fly  1475 NNLLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFLQNLEESLDLEPDFNKFEHCGQFRNV 1539
             ..|.:|:..::::..||...|.::|.|::||:.|||:||..||.:|.|..:..:
pombe   248 -EYLSNYMVILQQKQILSQDTILSIFTNLNEILDFQRRFLVGLEMNLSLPVEEQR---------- 301

  Fly  1540 LFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPKQQHSSTLESYLIKP 1604
               :|:.|:.....|.:|..||.:...||:::..|:     .:.|...| ..:.|..|.:.||||
pombe   302 ---LGALFIALEEGFSVYQVFCTNFPNAQQLIIDNQ-----NQLLKVAN-LLEPSYELPALLIKP 357

  Fly  1605 IQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQK 1669
            ||||.||||||.|:...|.:.......|.:.:..:.:||..:||.:||||...||.:  ..|
pombe   358 IQRICKYPLLLNQLLKGTPSGYQYEEELKQGMACVVRVANQVNETRRIHENRNAIIE--LEQ--- 417

  Fly  1670 SCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKERLRQKKKLMGV 1734
               :.||.....|.|:|   .|.:.|.:...|..:|.....::|:. ::..|||....|::...:
pombe   418 ---RVIDWKGYSLQYFG---QLLVWDVVNVCKADIEREYHVYLFEK-ILLCCKEMSTLKRQARSI 475

  Fly  1735 SSKNATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNSTADF 1799
            |....|..::.::.:..|..:.:.....: ..|.|:.:  .|..|...|..|   ::|.....:.
pombe   476 SMNKKTKRLDSLQLKGRILTSNITTVVPN-HHMGSYAI--QIFWRGDPQHES---FILKLRNEES 534

  Fly  1800 RNAFLKTIRQIIRESVRNMSIPMKNFGGSSGSVSGHSSQGMGSMGYPGNSQTLERPKQQITIVHG 1864
            ...::..:.:::.::.......:::...:..:...:.|....|.||..:...|.|.......|:.
pombe   535 HKLWMSVLNRLLWKNEHGSPKDIRSAASTPANPVYNRSSSQTSKGYNSSDYDLLRTHSLDENVNS 599

  Fly  1865 SHTLGKPKKKSGSQRHSAGNIDYDNLSGSQEADDLP---------PSVGVVHYASGHTHGQQMQP 1920
            ..::..|..||.....:...    :...:...|:.|         .|||.....:..|
pombe   600 PTSISSPSSKSSPFTKTTSK----DTKSATTTDERPSDFIRLNSEESVGTSSLRTSQT------- 653

  Fly  1921 AGFRGRSKTVGDVTEITCSSPEPHQQQQQQQQQQQQLMQQGHAHAHPHPHPHPREPPPPPIRQPH 1985
                 .|..|.:.:..|.|.|    .|..:..|...|:..                        :
pombe   654 -----TSTIVSNDSSSTASIP----SQISRISQVNSLLND------------------------Y 685

  Fly  1986 LHHHSSDIERIDPGTKSEGE-----EDSQQGT---------------IRPK-----------ATL 2019
            .::..|.|.|:..|| .:|.     ||:...|               :||:           .:|
pombe   686 NYNRQSHITRVYSGT-DDGSSVSIFEDTSSSTKQKIFDQPTTNDCDVMRPRQYSYSAGMKSDGSL 749

  Fly  2020 GRTPNHLTLSTTST---LSVGST 2039
            ..:..|.:||::||   |||.:|
pombe   750 LPSTKHTSLSSSSTSTSLSVRNT 772

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413
		PDZ_signaling	1221..1308	CDD:238492
		RhoGEF	1456..1647	CDD:238091	58/190 (31%)
		PH2_Tiam1_2	1650..1823	CDD:269957	31/172 (18%)
		PH	1693..1812	CDD:278594	18/118 (15%)
scd1	NP_594221.1	CDC24	105..194	CDD:283938
		RhoGEF	232..401	CDD:279015	57/188 (30%)
		PH_Scd1	404..546	CDD:270066	31/159 (19%)
		PB1	775..848	CDD:214770
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	81	1.000	Domainoid score	I2325
eggNOG	1	0.900	-	-		E1_KOG3519
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		oto101130
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1291
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.840