DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Vav1

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_036891.2	Gene:	Vav1 / 25156	RGDID:	3951	Length:	843	Species:	Rattus norvegicus

Alignment Length:	585	Identity:	122/585 - (20%)
Similarity:	225/585 - (38%)	Gaps:	150/585 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly  1440 STTALTPSRQLTDAEKLRKVVMELVDTERTYVKHLNNLLEHYLEPMKRETFLSNAEINALFGNIH 1504
            |.:..||.: :|:.:|....:.|:..||..|...|.::.:|:::|::|  ||...::..:|.||.
  Rat   180 SESVPTPPK-MTEYDKRCCCLREIQQTEEKYTDTLGSIQQHFMKPLQR--FLKPQDMETIFVNIE 241

  Fly  1505 EIVTFQRQFLQNLEESLDLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQK 1569
            |:::....||:.|:::|.           |....:|:.:   |:.|...|.:|..:|:....|.|
  Rat   242 ELLSVHTHFLKELKDALS-----------GPGATMLYQV---FIKYKERFLVYGRYCSQVESASK 292

  Fly  1570 VLHPNEGNHALQEFLAARNPKQ-------QHSS----TLESYLIKPIQRILKYPLLLQQM-RNLT 1622
                    | |.:...||...|       |.::    ||...|:.|:||:|||.||||:: ::..
  Rat   293 --------H-LDQVATAREDVQMKLEECSQRANNGRFTLRDLLMVPMQRVLKYHLLLQELVKHTQ 348

  Fly  1623 DTRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAI------FDHLFRQHQKSCKQPIDLSPGD 1681
            ||...|::.|  ||..|..:|:.:||::|.:|....|      .::|.:......:..||   |:
  Rat   349 DTTEKENLRL--ALDAMRDLAQCVNEVKRDNETLRQITNFQLSIENLDQSLANYGRPKID---GE 408

  Fly  1682 LLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKERLRQKKKLMGVSSKNATNEVEII 1746
            |.             :..:::..:.....|:...|:: :||.|        |.|
  Rat   409 LK-------------ITSVERRSKTDRYAFLLDKALL-ICKRR--------GDS----------- 440

  Fly  1747 RYQVLIPVT--EVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNSTADFRNAFLKTIRQ 1809
             |.:...|.  ..|||..|:.:.|:             ::.|....::.:..|.....|.|| |:
  Rat   441 -YDLKASVNLHSFQVRDDSSGERDN-------------KKWSHMFLLIEDQGAQGYELFFKT-RE 490

  Fly  1810 IIRESVRNMSIPMKNFGGSSGSVSGHSSQ------------------GMGSMGY-------PGNS 1849
            :.::.:....:.:.|....:.:.:||..|                  |....||       |.:.
  Rat   491 LKKKWMEQFEMAISNIYPENATANGHDFQMFSFEETTSCKACQMLLRGTFYQGYRCYRCRAPAHK 555

  Fly  1850 QTLER----PKQQITIVHGSHTLGKPKKKSGSQRHSAGNIDYDNLSGSQEADDLPPSVGVVHYAS 1910
            :.|.|    .:|..     |.|:.|.|....:|......:....:...||...:||..|    |.
  Rat   556 ECLGRVPPCGRQDF-----SGTMKKDKLHRRAQDKKRNELGLPKMEVCQEYYGIPPPPG----AF 611

  Fly  1911 GHTHGQQMQPAGFRGRSKTVGDVTEITCSSPEPHQQQQQQQQQQQQLMQQGHAHAHPHPHPHPRE 1975
            |..  .::.|          ||:.|:|.:..| |...:.:.....::.........|:.|..|::
  Rat   612 GPF--LRLNP----------GDIVELTKAEAE-HNWWEGRNTATNEVGWFPCNRVRPYVHGPPQD 663

Fly 1976 1975
Rat 664 663

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413
		PDZ_signaling	1221..1308	CDD:238492
		RhoGEF	1456..1647	CDD:238091	54/202 (27%)
		PH2_Tiam1_2	1650..1823	CDD:269957	28/180 (16%)
		PH	1693..1812	CDD:278594	20/120 (17%)
Vav1	NP_036891.2	CH	2..115	CDD:278723
		RhoGEF	195..371	CDD:238091	54/202 (27%)
		PH_Vav	385..508	CDD:269930	26/173 (15%)
		PH	403..504	CDD:278594	24/151 (16%)
		C1_1	516..564	CDD:278556	8/47 (17%)
		SH3_VAV1_1	594..656	CDD:212912	14/78 (18%)
		SH2_Vav1	663..765	CDD:198268	0/1 (0%)
		SH3_VAV1_2	784..837	CDD:212909
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.910