DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Ngef

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_038938943.1	Gene:	Ngef / 246217	RGDID:	1309055	Length:	700	Species:	Rattus norvegicus

Alignment Length:	531	Identity:	106/531 - (19%)
Similarity:	187/531 - (35%)	Gaps:	143/531 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly  1333 CPPPPTDPPVMSEEMITGLIVPAPGWNGTSKD---LYSPEAESSPATSFVDPAAMAAQLAVGGVG 1394
            ||.....|..::|.:.....:||..|....:.   ||....:.|............|::. |...
  Rat   135 CPALTDSPTTLTEALQMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIQ-GNSD 198

  Fly  1395 GVVVGGLGVAKPTSRTSSFEIENLLKTAEQETRKSSP------------------------TGSV 1435
            |...|.               :|..:..|:|...:||                        ...:
  Rat   199 GSQAGE---------------DNAEEEEEEEEEPASPPERRALPQICLLSNPHSRFNLWQDLPEI 248

  Fly  1436 TSSVSTTALTPSRQLTDAEKLRKVVMELVDTERTYVKHLNNLLEHYLEPMKRETFLSNAEINALF 1500
            .||.....|.|     :..||::.:.|||.:|.:|.|.||.|:.|::|..:.:..|..:|.:.||
  Rat   249 QSSGVLDILQP-----EETKLQEAMFELVTSEASYYKSLNLLVSHFMENERLKKILHPSEAHILF 308

  Fly  1501 GNIHEIVTFQRQFLQNLEESLDLEPDFNKFEHCGQFRNVLFA--IGSAFLYYVNHFKLYSSFCAS 1563
            .|:.:::....:||..||..::              .|::.:  ....:.|..:||.:|.::.::
  Rat   309 SNVLDVMAVSERFLLELEHRME--------------ENIVISDVCDIVYRYAADHFSVYITYVSN 359

  Fly  1564 HSKAQKVLHPNEGNHALQEFLAAR--------NPKQQHSSTLESYLIKPIQRILKYPLLLQQMRN 1620
            .:..::..     ...|||..|.|        :||.: ...|.|:||.|.|||.:..||:|.:
  Rat   360 QTYQERTY-----KQLLQEKTAFRELIAQLELDPKCK-GLPLSSFLILPFQRITRLKLLVQNI-- 416

  Fly  1621 LTDTRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQPIDLSPGDLLYY 1685
                           ||.:|:.:|........|:|...:.        |:|.:
  Rat   417 ---------------LKRVEEGSEREGTALDAHKELEMVV--------KACNE------------ 446

  Fly  1686 GGVEWLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKERLRQKKKLMGVS---------SKNATN 1741
             ||..::.::.:..|:|.:|     |..||..:......|.::.:|..:|         :|....
  Rat   447 -GVRKMSRTEQMISIQKKME-----FKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFR 505

  Fly  1742 EVEIIRYQVL------IPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNSTADFR 1800
            |:.:..:..|      ||..:.||..|:.:.     |..:..|..|.|..:....:.....||.|
  Rat   506 EIYLFLFNDLLVICRQIPGDKYQVFDSAPRG-----LLRVEELEDQGQTLANVFILRLLENADDR 565

  Fly  1801 NA--FLKTIRQ 1809
            .|  .||...|
  Rat   566 EATYMLKASSQ 576

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413
		PDZ_signaling	1221..1308	CDD:238492
		RhoGEF	1456..1647	CDD:238091	48/200 (24%)
		PH2_Tiam1_2	1650..1823	CDD:269957	34/177 (19%)
		PH	1693..1812	CDD:278594	28/134 (21%)
Ngef	XP_038938943.1	RhoGEF	267..445	CDD:395496	50/222 (23%)
		PH_ephexin	467..593	CDD:269929	24/115 (21%)
		SH3_ephexin1	606..660	CDD:212872
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910