DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Farp2

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_663494.2	Gene:	Farp2 / 227377	MGIID:	2385126	Length:	1065	Species:	Mus musculus

Alignment Length:	1244	Identity:	244/1244 - (19%)
Similarity:	398/1244 - (31%)	Gaps:	411/1244 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly   890 KGYWVCLKGTTLLFYPCDSREGRSVEAAPKHLIIVDGAIMQPIPEHPKRDYIFCLSTAFGDAYLF 954
            :.||:.|:....:.     |:.|..:.|...|.:   ....|.|...:.:|         ..|||
Mouse    93 QSYWIWLEPMKPII-----RQVRKPKNAVLRLAV---KFFPPDPGQLQEEY---------TRYLF 140

  Fly   955 QAPCQVELENWVNSIHSACAAAFARHRGKTGTL---HLLQEEIFRLEKAIESDHKLK---HMAEL 1013
                .::|:..:......|.|       .|..|   ||||.||...::.::.:| ||   ::...
Mouse   141 ----ALQLKRDLLEERLTCTA-------NTAALLISHLLQSEIGDYDETLDREH-LKANEYLPNQ 193

  Fly  1014 QQSV--VTDQETRHQIQT------QILQWEENLERLHCEQFRLRCYMASLQSGELPNPKSLLTHV 1070
            ::|:  :.|...||..||      |:|:....|     |.:.:|.:|||.:.|...|  ..::|:
Mouse   194 EKSLEKILDFHQRHTGQTPAESDFQVLEIARKL-----EMYGIRFHMASDREGTKIN--LAVSHM 251

  Fly  1071 SRPTKNTLNKLGVFTVSSFHAFICARSPSLLNNLLAGRGATKRRPPMLSRSNSGSSRRSMQ--MN 1133
            .........|:..|..|........|...|:          |..|.:     .|..:.:::  :.
Mouse   252 GVLVFQGTTKINTFNWSKVRKLSFKRKRFLI----------KLHPEV-----HGPYQDTLEFLLG 301

  Fly  1134 SRDEPEKTFKVAMPDNAYSTVY-LRDAMSVEE----FLASACARRNLNPMEHFVRVKKRRDMEDH 1193
            ||||.:..:|:.:   .|.|.: |.|....:.    |...:..|.:....:..|...|...|:.
Mouse   302 SRDECKNFWKICV---EYHTFFRLSDQPKPKAKAVFFSRGSSFRYSGRTQKQLVDYVKDGGMKR- 362

  Fly  1194 NYFVPHRNDLIENYLHNHEFVEVCMKILYQVELQRTTLEQMWGFSVEAELIENAERQDELCCYVS 1258
               :|        |...|......:..| .|:|.:
Mouse   363 ---IP--------YERRHSKTRTSLHAL-TVDLPK------------------------------ 385

  Fly  1259 RVEDKSVAMHNGIIKGDEIMVINGAIVSDLDMMYLESVLQEEQSLSMMMRSSRTEPPDLVGIMRV 1323
                :||:..:|:        ...|.:|..::.:...            .||...||.|..:
Mouse   386 ----QSVSFTDGL--------RTSASLSSANVSFYPP------------PSSSLSPPGLPNL--- 423

  Fly  1324 TDDMIDSLVCPPPPTDPPVMSEEMITGLIVPAPGWNGTSKDLYSPEAESSPATSFVD-PAAMAAQ 1387
             .|...|||.|..|.                          :.|..||.|...|..| |:..:|.
Mouse   424 -KDSSSSLVDPQAPV--------------------------IKSTAAERSSGPSSSDGPSTQSAH 461

  Fly  1388 LAVGGVGGVVV---GGLGVAKPTSRTSSF--------EIENLLKTAEQETRKSSPTGSVTSSVST 1441
            |.    |..|:   .|..:..|....||.        |::..|.||||   .:||..|...|.:.
Mouse   462 LP----GPPVLRPGPGFSMDSPQPSPSSLKSHLSLCPELQAALSTAEQ---GASPVLSPVLSGAG 519

  Fly  1442 TALTPSRQLTDAEKLRK---------VVMELVDTERTYVKHLNNLLEHYLEPMKRETFLSNAEIN 1497
            ||    |.....|:..|         :..|::.|||||:|.|..:...:...:.:|..:..|.:.
Mouse   520 TA----RMDNQEEQKHKHMPEDEAYFIAKEILATERTYLKDLEVITVWFRSVLIKEEAMPAALMA 580

  Fly  1498 ALFGNIHEIVTFQRQFLQNLEESLDLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCA 1562
            .||.||..:..|.|.||..:|:.|.|....:.....|..:.    ||...|..:...|.::|:..
Mouse   581 LLFSNIDPVYEFHRGFLHEVEQRLALWEGPSSAHLKGDHQR----IGDILLRNMRQLKEFTSYFQ 641

  Fly  1563 SHSKAQKVLHPNEGNHALQEFLAARNPKQQHSSTLE-----------------SYLIKPIQRILK 1610
            .|                .|.|.......:|...||                 ::|:||:||::.
Mouse   642 RH----------------DEVLTELEKATKHCKKLEAVYKEFELQKVCYLPLNTFLLKPVQRLVH 690

  Fly  1611 YPLLLQQM--------RNLTDTRADEHVHLCEALKGMEKVAEHINE-MQRIHEEYGAIFDHLFRQ 1666
            |.|||.::        |:..|..        ||||.:.:|...:.: :.|:        ::|
Mouse   691 YRLLLSRLCAHYSPGHRDYADCH--------EALKAITEVTTELQQSLTRL--------ENL--- 736

  Fly  1667 HQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKE-------R 1724
             ||..:...||...:.|...|.|::.........||||: ..|.|:|...:::..|.       |
Mouse   737 -QKLTELQRDLVGVENLIAPGREFIREGCLHKLTKKGLQ-QRMFFLFSDMLLYTSKSVTGASHFR 799

  Fly  1725 LRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKV 1789
            :|....|.|:..:.:.||                              |.:.|..:..  .::|.
Mouse   800 IRGFLPLRGMLVEESENE------------------------------WSVPHCFTIY--AAQKT 832

  Fly  1790 YVLSNSTADFRNAFLKTIRQIIRESVRNMSIPMKNFGG--------SSGSVS-GHSSQGMGSMG- 1844
            .|::.||...:..:::.:...|:.:......|....||        ||..|| ..|..|.|:.|
Mouse   833 IVVAASTRLEKEKWMQDLNAAIQAAKTIGDSPPVLLGGPVYTRTPRSSDEVSLEESEDGRGNRGS 897

  Fly  1845 YPGNSQ---------------TLERPKQQITIVH--GSHTLGKPKKKSGSQR------------H 1880
            ..||||               ::.|......:.:  ..:.|.|.|..:|.|:            :
Mouse   898 LEGNSQHRANTTMHVCWYRNTSVSRADHSAAVENQLSGYLLRKFKNSNGWQKLWVVFTNFCLFFY 962

  Fly  1881 SAGNIDYD---------NLSGSQEADDLPPSVGVVHYASGHTHGQQMQPAGFRGRSKTV----GD 1932
            .....||.         ::|..:|||.:............|.:       .||..||..    .|
Mouse   963 KTHQDDYPLASLPLLGYSVSLPREADSIHKDYVFKLQFKSHVY-------FFRAESKYTFERWMD 1020

  Fly  1933 VTEITCSSPEPHQQQQQQQQQQQQLMQQGHAHAHPHPHPHPREPPPPPIRQPHLHHHSSDIERID 1997
            |.:...|||                   |.               ||...|...||        .
Mouse  1021 VIKRASSSP-------------------GR---------------PPSFTQDCSHH--------S 1043

  Fly  1998 PGTKSEGEE 2006
            ||.::|..|
Mouse  1044 PGLEAEIRE 1052

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	16/94 (17%)
		PH	873..974	CDD:278594	14/83 (17%)
		TIAM1_RBD	1141..1219	CDD:176413	13/82 (16%)
		PDZ_signaling	1221..1308	CDD:238492	8/86 (9%)
		RhoGEF	1456..1647	CDD:238091	50/224 (22%)
		PH2_Tiam1_2	1650..1823	CDD:269957	31/179 (17%)
		PH	1693..1812	CDD:278594	20/125 (16%)
Farp2	NP_663494.2	B41	45..234	CDD:214604	38/174 (22%)
		FERM_N	48..111	CDD:286467	4/22 (18%)
		FERM_M	131..234	CDD:278785	29/128 (23%)
		FERM_C_FARP1-like	221..341	CDD:270014	27/144 (19%)
		FA	333..375	CDD:285894	8/53 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	406..489		26/128 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	504..534		11/36 (31%)
		RhoGEF	540..723	CDD:238091	48/210 (23%)
		PH1_FARP1-like	752..859	CDD:269928	24/139 (17%)
		PH	760..854	CDD:278594	20/126 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	874..902		9/27 (33%)
		PH2_FARP1-like	929..1026	CDD:270055	18/103 (17%)
		PH	934..1027	CDD:278594	18/99 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910