DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Arhgef4

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_001355702.1	Gene:	Arhgef4 / 226970	MGIID:	2442507	Length:	1855	Species:	Mus musculus

Alignment Length:	1752	Identity:	331/1752 - (18%)
Similarity:	571/1752 - (32%)	Gaps:	535/1752 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly   375 INNNTRRKTKSTEDMNVDTSTLKRM-----------LKPMPSTESPVTSP--------------- 413
            :.|...:.::|....:..||.|.::           .||..|.||.:..|
Mouse   393 LENQVEQASRSCPAPSHLTSELLKLNSQEEVPRPAECKPGHSPESRLEEPKGVHPDDVISKQQCL 457

  Fly   414 -----EMGRRRYNYYNANAAQTLGHPPHMHQ-------HGMAMGMGGGGGGGHHIMNNNTMGRAS 466
                 |.|.:..||.:.:|.|....||....       ||..........|.....:::.....:
Mouse   458 LNISGEAGSQTENYPSMSALQEERKPPDRASLFPQHCCHGEVPVCNRMLAGSWEEESDSACAPRT 522

  Fly   467 SQSSRFSGSRSSHEIGRGYPPRNLYLELERERSCIEG--SPPSDNVMFDN---QCYATTPSSSNG 526
            :..|...||..:.|.|       :.|:|:|....::.  ..|:..|...|   :|     :...|
Mouse   523 ALKSSVIGSLEAAEEG-------VVLDLQRNGKALQSLLDFPTLEVSHRNPGSEC-----AQGPG 575

  Fly   527 NSDQDQSYGQQQSSGQH------------PQQQQGPPQRSSRHQHHHQQAPNVTPTPGSPTSRLL 579
            :..:....||:|:.|.|            ..:|:|  :::|..:.|....|.....|...||  .
Mouse   576 DDPEPSPSGQRQAPGAHGASRHSCKFIMIAVKQEG--RQTSTRKAHCLIEPQCGHLPEKGTS--A 636

  Fly   580 LEYEMHLRNTL-----------AKGMDAESYSLHTFEALLSQSMENLEFAESLPGSNQRSPYPLR 633
            ..||....:.:           ..|.:.:..:|.    ||:|.:|::.    :|.|...|...|
Mouse   637 TAYESSRASEMPVFGNECELLGCPGAEVDGGALQ----LLAQGIEHVR----VPVSRTSSDDTL- 692

  Fly   634 RPNAKSSTLPLPPHRPLSTIRDKERDRDRDGYYSDRNELIRERERERDRGYLSDHNSSFSNSRCA 698
               :...||.       ..:..:..|...:..::::|:.:...:|:|.|....|    ..|:...
Mouse   693 ---SSEETLE-------GELLGERLDLGSECVHTEQNQELPSSDRKRGRCGTGD----AENAEIC 743

  Fly   699 SCIGESARAQWFRHSDGWRSGSSTIGSGSGHGMMTQQIPGSGHKRSPWDSLPSLRQDSSLNDSGY 763
            :|.||.             .|..:....|....:..|.|..|.::|..:|
Mouse   744 ACAGEV-------------EGPLSRVLASSTTTLEFQEPCGGFQQSVLES--------------- 780

  Fly   764 KSARADSLEQRAEFIRQDSLRSEYLSDRESRYGIVQQASIESTDSRMCYLTSSEI-----SDDDR 823
                    ||....::...||::....||...|.:..:..         |||.::     |.::|
Mouse   781 --------EQHPACLQMTPLRTDMPWSREMPGGDIPYSQA---------LTSEDLNPVCPSPEER 828

  Fly   824 ------------------MSLTT-------AVSDEDDGESVMASPYKAKATGTAASSFNCTGAVR 863
                              :||.|       ..|.:..||......|..|...|..|......|:.
Mouse   829 VPHSPCQGEPPLHHVHQSVSLETKSGVIQVGTSGDLSGEGPSPESYNIKRLKTTESKIKARLALA 893

  Fly   864 KAGFLSVKKWLLRKKHQI-----ELARKRGWKGYWVCLKGTTLLFYPCDSREGRSVEAAPKHLII 923
            ...|.:..:..:.:|...     |..::|..:|.|       ..|......||      .|...:
Mouse   894 HKTFTNFFEAKVMEKTNTGSVKGEKEKRRRARGSW-------RAFLRSKDAEG------SKRPAL 945

  Fly   924 VDGAIMQPIPEHPKRDYIFCLS-----------TAFGDAYLFQAPCQVELENW--VNS--IHSAC 973
            |.|.   |.|..|:   :.|.|           |...|:::|.       |:|  :||  ..|:.
Mouse   946 VSGV---PEPGFPQ---LLCSSPPATRGHCEKWTIDTDSHVFG-------EHWTPLNSPATLSSS 997

  Fly   974 AAAFARHRGKTGTLHLLQEEIFRLEKAIESDHKLKHMAELQQSVVTDQETRHQIQTQILQWEENL 1038
            ..|...||.::       |...|...|.||...|......:.|.:|...:....|..|
Mouse   998 HLASPEHRRRS-------EPTIRRTAAQESSTHLSSGVFPKSSWLTSATSPGAAQIDI------- 1048

  Fly  1039 ERLHCEQFRLRCYMASLQSGE---------LPNPKSLLTHVSRPTKNTLNKLGVFTVSSFHAFIC 1094
                  .|.|.|..|...:.|         .|.|:|.:....|..                  :|
Mouse  1049 ------SFALPCSSACHLTYENQGMPCRPTSPKPRSPMAGAQRMD------------------LC 1089

  Fly  1095 ARS-------PSLLNNLLAGRGATKRRPPMLSRSNSGSSRRSMQMNSRDEPEKTFKVAMPDNAYS 1152
            .|.       .||.::..|.|.:.....|..|::.:........:|..|:.|...:........:
Mouse  1090 LRGRTSAVSMVSLRSDKDANRSSEVPERPKASKARASLLLSLQTLNQNDQKEDKSQCQCYHGLGT 1154

  Fly  1153 TVYLRDAMSVEEFLASACARRNLNPMEHFVRVKKRRDMEDHNYFV--PHRNDLIENYLHNHEFVE 1215
            ..:|||....|.::|..      :|.:     |||....|...:.  |||.
Mouse  1155 VPFLRDHPGNESYIAQE------SPPD-----KKRNCSHDQKTYTEPPHRP-------------- 1194

  Fly  1216 VCMKILYQVELQRTTLEQMWGFSVEAELIENAERQDELCCYVSRVEDKSVAMHNGIIKGDEIMVI 1280
                 :...|:.| ||..|....|..|:               .::.:....|:..
Mouse  1195 -----VSATEVTR-TLPSMSAEQVPREV---------------PLQFRRAPQHSHF--------- 1229

  Fly  1281 NGAIVSDLDMMYLESVLQEEQSLSMMMR---SSRTEPPDLVGIMRVTDDMIDSLVCPPPPTDPPV 1342
                  ..|.::||...:.:....|::|   .:...|.|.|...|.|  .|.|...|..|....:
Mouse  1230 ------SFDDIWLEKAQRRKLKKQMLLRRQMCAELVPEDPVQCWRKT--TITSPESPHLPRRSHL 1286

  Fly  1343 MSEEMITGLIVPAPGW---------------------NGTSKDLYSPEAESSPATSFVDPAAMAA 1386
            :|:....||  ...||                     .|:.:|||  |...:....:..|.....
Mouse  1287 LSQSAPMGL--DHTGWPEPTSDTGMPDGTLDTVCAEETGSEEDLY--EELHNSGHHYSHPRGGGE 1347

  Fly  1387 QLAVGGV---GGVVVG------------GLG--------VAKPTSR--------------TSSFE 1414
            |||:..:   |.||..            .||        |...|:|              .:||.
Mouse  1348 QLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFV 1412

  Fly  1415 IENLLKTAEQE-----------TRKSSPTGSVTSSVSTTALTPSRQLTDAEKLRKVVMELVDTER 1468
               .|:..:.|           .|::..:|            |..|....:....|:.|::.|||
Mouse  1413 ---RLRVNQDEPADDYEAPRAGAREADDSG------------PEAQSCKDQMRTNVINEILSTER 1462

  Fly  1469 TYVKHLNNLLEHYL-EPMKRETFLSNAEINALFGNIHEIVTFQRQFLQNLEESLDLEPDFNKFEH 1532
            .|:|||.::.|.|: :..|||...|..::..:||||.:|...|:.|::.||:..:.|
Mouse  1463 DYIKHLRDICEGYVRQCRKREDMFSEEQLRTIFGNIEDIYRCQKAFVKALEQKFNTE-------- 1519

  Fly  1533 CGQFRNVLFAIGSAFLYYVNHFKLYSSFCASH----------SKAQKVLHPNEGNHALQEFLAAR 1587
                |..|..:|:.||.:...|::||.:|.:|          :|..|.::..|....||..:
Mouse  1520 ----RPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQRMI--- 1577

  Fly  1588 NPKQQHSSTLESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQRI 1652
                  ..:|:.:|:.|:|:|.||||.|.::...|..:..:..::..||..|:.||:.|||.:|.
Mouse  1578 ------DISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKNVEAALHAMKNVAQLINERKRR 1636

  Fly  1653 HEEYGAIFDHLFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISDFL--GKIKKGLELHA-----MC 1710
            .|....|     .|.|.|.:   |....|||       :..|:.:  |::.:..:..|     |.
Mouse  1637 LENIDKI-----AQWQSSIE---DWEGEDLL-------VRSSELIHSGELTRVTQPQARSQQRMF 1686

  Fly  1711 FVFKSAVVFLCKERLRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQ-VRASSAKDMDSHFLWE 1774
            |:|...:::..|:.||:                :::.|:..:.:.::: |.....||.|.|
Mouse  1687 FLFDRQLIYCKKDLLRR----------------DVLYYKGRLDMDDLEVVDVEDGKDRDLH---- 1731

  Fly  1775 LIHLRSQLQ---RRSEKVYVLSNSTADFRNAFLKTIRQIIRESVR---NMSIPMKNFGGSSGSVS 1833
             :.:::..:   ..:...::|.....:.:..:||...: .||.||   .....:.........::
Mouse  1732 -VSVKNAFRLYCGTTGDSHLLCARKPEQKQRWLKAFAR-EREQVRLDQETGFSITELQRKQAMLN 1794

  Fly  1834 GHSSQGMG---SMGYPG-------NSQTLERPKQQITIVHGSHTLGKPKKKSGSQRHSAGNI 1885
            ....|..|   ::|.||       .|....||:||:.:      |.:|::|..:..||...:
Mouse  1795 ASKQQATGKPKAVGRPGYLTRHKHPSLPASRPQQQVLV------LAEPRRKPSNFWHSISRL 1850

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	29/144 (20%)
		PH	873..974	CDD:278594	24/120 (20%)
		TIAM1_RBD	1141..1219	CDD:176413	13/79 (16%)
		PDZ_signaling	1221..1308	CDD:238492	12/86 (14%)
		RhoGEF	1456..1647	CDD:238091	56/201 (28%)
		PH2_Tiam1_2	1650..1823	CDD:269957	32/186 (17%)
		PH	1693..1812	CDD:278594	18/129 (14%)
Arhgef4	NP_001355702.1	SH3_ASEF	1344..1416	CDD:212906	14/74 (19%)
		RhoGEF	1450..1631	CDD:238091	56/201 (28%)
		PH_Collybistin_ASEF	1637..1776	CDD:269931	31/175 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3519
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1291
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.840