DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and SPATA13

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_001273721.1	Gene:	SPATA13 / 221178	HGNCID:	23222	Length:	1339	Species:	Homo sapiens

Alignment Length:	1632	Identity:	311/1632 - (19%)
Similarity:	519/1632 - (31%)	Gaps:	564/1632 - (34%)

- Green bases have known domain annotations that are detailed below.

  Fly   392 DTSTLKRMLKPMPSTESPVTSPEMGRRRYNYYNANAAQTLGHPPHMHQHGMAMGMGGGGGGGHHI 456
            ||.|.:..|.|              .:....::.:..:|..||...|    :|.:.|.....:.:
Human   121 DTDTQEAKLSP--------------AKLVRLFSTSRKRTGAHPERPH----SMVLVGNSSTWNTL 167

  Fly   457 MNNNTMG----------------RASSQSSRFSGSRSSHEIGRGYP----PRNLYLELERERSCI 501
            .:...||                ..|:..|:  |..|.|.:|:..|    |         .....
Human   168 ASFRKMGSFKKLKSSVLKGIQSREGSNACSK--GEASEHGLGKSIPNGAVP---------GAQAS 221

  Fly   502 EGSP----PSDNVMFDNQCYATTPS---SSNGNSDQDQSYGQQQSSGQHPQQQQGPPQRSSRHQH 559
            .|||    |:        |.|..|:   :.:|:..:|.....|:|:.:         .||.|..:
Human   222 RGSPLAPGPA--------CGALRPAEWGTLDGSDLEDTDDAFQRSTHR---------SRSLRRAY 269

  Fly   560 HHQQ-----APNVTPTPGSPTSRLLLEYEMHLRNTLAKGMDAESYSLHTFEALLSQSMENLEFAE 619
            ...:     ||....||...|.::....|:.:|       |.|:.|:....   |:|.:||.|
Human   270 GLGRICLLDAPQNHATPTIATGQVPAVCEILVR-------DPENNSMGYRR---SKSTDNLAF-- 322

  Fly   620 SLPGSNQRSPYPLRRPNAK-SSTLPLPPHR-------PLSTIRDKERDRDRDGYYSDRNELIRER 676
                        |::.:.| .||..|...|       |..|:.....|..:.|  |.|.:..|..
Human   323 ------------LKKSSFKRKSTSNLADLRTAHDARVPQRTLSSSSTDSQKLG--SGRTKRWRSP 373

  Fly   677 ERERDRGYLSDHNSSFSNSRCASCIGESARAQWFRHSDGWRSGSSTIGSGSGHGMMTQQIPGSGH 741
            .|.:|            ..|....:.....|.|.|.|.  |||:.:              ||...
Human   374 IRAKD------------FDRVFKLVSNVTEAAWRRESP--RSGAPS--------------PGEAS 410

  Fly   742 KRSPWDSLPSLRQDSSLNDSGYKSARADSLEQRAEFIRQDSLRSEYLSDRESRYGIVQQASIEST 806
            .|        |:..|.|:|. |....:.|.|       |||.|.          |.|...:
Human   411 LR--------LQAHSRLHDD-YSRRVSRSTE-------QDSRRG----------GAVMHGT---- 445

  Fly   807 DSRMCYLT---SSEISDDDRMSLTTAVSDEDDGESVMASPYKAKATGTAASSFNCTGAVRKAGFL 868
             :..|.:.   .|..|....:...|||           .|.:.|::....|..:||.:...:..:
Human   446 -TATCTVAPGFGSATSKGPHLDADTAV-----------FPLETKSSWAVESDSSCTCSSLPSPIV 498

  Fly   869 SVKKWLLRKKHQIELARKRGWKGYWVCLKGTTLLFYPCDSREGRSV----EAAPKHLIIVDGAIM 929
                       |..|::.                  .||...|..:    |..|..|     ...
Human   499 -----------QDVLSKD------------------SCDPNAGSQLTFDPEQPPTPL-----RPT 529

  Fly   930 QPIPEHPKRDYIFCLSTAFGDAYLFQAPCQVELENWVNSIHSACAAAFARHRGKTGTLHLLQEEI 994
            .|.|:.|:...    |...|     .|.|        :|.|||.:|......|:.......:...
Human   530 TPKPQSPQSPQ----SPGAG-----SASC--------HSNHSALSANSEESEGRAEEPAQREPGP 577

  Fly   995 FRLEKAIESDHKLKHMAEL--------------QQSVVTDQETRHQIQTQILQWEENLERLHCEQ 1045
            ..|:..:|:.|..:...:|              ::.||.|...|....    |.||..|.....:
Human   578 VSLQDPLEATHGDEGSKDLLVNIGVAAGPEEKEKEEVVPDGPWRRSSS----QDEERTEAQRTPK 638

  Fly  1046 FRLRCYMASLQSGELPNPKSLLTHVSRPTKNTLNKLGVFTVSSFHAFICARSPSLLNNLLAG--- 1107
            .|       ..||..|.|:..                     |.:..:.:||.|:..:.:|.
Human   639 RR-------WGSGRRPRPRPF---------------------SDYGQLASRSLSIPEDSVAADPQ 675

  Fly  1108 ----------------------------RGATKRRPPMLSRSNS--GSSRRSMQMNSRDEPEKTF 1142
                                        :||.:|||..:....|  |:::.....|...:|..
Human   676 KEDRVDEDPQASMTSASPEDQNAPVGCPKGARRRRPISVIGGVSLYGTNQTEELDNLLTQPAS-- 738

  Fly  1143 KVAMPDN---AYSTVYLRDAMSVEEFLASACARRNLNPMEHFVRVKKRRDMEDHNYFVPHRNDLI 1204
            :..||.:   .|..|..|    ...|..|......|:      ||.:||.|...|
Human   739 RPPMPAHQVPPYKAVSAR----FRPFTFSQSTPIGLD------RVGRRRQMRASN---------- 783

  Fly  1205 ENYLHNHEFVEVCMKILYQVELQRTTLEQMWGFSVEAELIENAERQDELCCYVSRVEDKSVAMHN 1269
                                      :....|....|.:.:|...:|      ...||...|...
Human   784 --------------------------VSSDGGTEPSALVDDNGSEED------FSYEDLCQASPR 816

  Fly  1270 GIIKGDEIMVINGAIVSDLDMMYLES----VLQEEQSLSMMMRSSRTEPPDLVGIMRVTDDMIDS 1330
            .:..|.|.:.|| .::||.:::..|:    |..::|.|..       :..|::.::..::
Human   817 YLQPGGEQLAIN-ELISDGNVVCAEALWDHVTMDDQELGF-------KAGDVIQVLEASN----- 868

  Fly  1331 LVCPPPPTDPPVMSEEMITGLIVPAPGWNGTSKDLYSPEAESSPATSFVDPAAMAAQLAVGGVGG 1395
                                    ...|.|.|:|     .|:....|||
Human   869 ------------------------KDWWWGRSED-----KEAWFPASFV---------------- 888

  Fly  1396 VVVGGLGVAKPTSRTSSFEIENLLKTAEQETRKSSPTGSVTSSVSTTALTPSRQLTDAEKLR-KV 1459
                                  .|:..::|..::|.:..........:.:..|...:.:::| .|
Human   889 ----------------------RLRVNQEELSENSSSTPSEEQDEEASQSRHRHCENKQQMRTNV 931

  Fly  1460 VMELVDTERTYVKHLNNLLEHYLEPMKRET-FLSNAEINALFGNIHEIVTFQRQFLQNLEESLDL 1523
            :.|::||||.|:|||.::.|.|:...::.| ..:.|::..:||||.:|..|||:||:      ||
Human   932 IREIMDTERVYIKHLRDICEGYIRQCRKHTGMFTVAQLATIFGNIEDIYKFQRKFLK------DL 990

  Fly  1524 EPDFNKFE-HCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKA----QKVLHPNEGNHALQEF 1583
            |..:||.| |       |..|||.||.....|.:||.:|.:|..|    ..::...:..|.   |
Human   991 EKQYNKEEPH-------LSEIGSCFLQNQEGFAIYSEYCNNHPGACLELANLMKQGKYRHF---F 1045

  Fly  1584 LAARNPKQQHSSTLESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINE 1648
            .|.|..:|.....::.:|:.|:|:|.||||.|.::...|.....::.::..|.:.|:.||..|||
Human  1046 EACRLLQQMIDIAIDGFLLTPVQKICKYPLQLAELLKYTTQEHGDYSNIKAAYEAMKNVACLINE 1110

  Fly  1649 MQRIHEEYGAIFDHLFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISD---------FLGKI-KKG 1703
            .:|..|..           .|..:..:.:.        |.|.|:|.|         .|.|| |:|
Human  1111 RKRKLESI-----------DKIARWQVSIV--------GWEGLDILDRSSELIHSGELTKITKQG 1156

  Fly  1704 LELHAMCFVFKSAVVFLCKERLRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQ-VRASSAKDM 1767
            .......|:|...:|...|:.||:                :::.|:..:.:.|:: |.....:|.
Human  1157 KSQQRTFFLFDHQLVSCKKDLLRR----------------DMLYYKGRLDMDEMELVDLGDGRDK 1205

  Fly  1768 DSHF----LWELIHLRSQLQRRSEKVYVLSNSTADFRNAFLKTI----RQIIRESVRNMSIP--M 1822
            |.:.    .::|:      .|.:::||:......:.:..:|:..    |::..:....|.|.  .
Human  1206 DCNLSVKNAFKLV------SRTTDEVYLFCAKKQEDKARWLQACADERRRVQEDKEMGMEISENQ 1264

  Fly  1823 KNFGGSSGSVSGHSSQGMGSMGYPGNSQTLERPKQQITIVHGSHT-------------LGKPKKK 1874
            |.....:...:||..    |.||  |...:..|.|.:..:|..|.             |.:||:|
Human  1265 KKLAMLNAQKAGHGK----SKGY--NRCPVAPPHQGLHPIHQRHITMPTSVPQQQVFGLAEPKRK 1323

  Fly  1875 SGSQRHS 1881
            |....|:
Human  1324 SSLFWHT 1330

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	21/128 (16%)
		PH	873..974	CDD:278594	19/104 (18%)
		TIAM1_RBD	1141..1219	CDD:176413	14/80 (18%)
		PDZ_signaling	1221..1308	CDD:238492	17/90 (19%)
		RhoGEF	1456..1647	CDD:238091	63/197 (32%)
		PH2_Tiam1_2	1650..1823	CDD:269957	32/193 (17%)
		PH	1693..1812	CDD:278594	24/137 (18%)
SPATA13	NP_001273721.1	SH3_ASEF	821..892	CDD:212906	20/150 (13%)
		RhoGEF	931..1110	CDD:279015	63/194 (32%)
		PH_Collybistin_ASEF	1115..1253	CDD:269931	29/178 (16%)
		PH	1143..1242	CDD:278594	21/120 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1291
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.940