DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Itsn2

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_001185897.1	Gene:	Itsn2 / 20403	MGIID:	1338049	Length:	1685	Species:	Mus musculus

Alignment Length:	1604	Identity:	306/1604 - (19%)
Similarity:	549/1604 - (34%)	Gaps:	412/1604 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly   394 STLKRMLKPMPSTESPVTSPEMGRRRYNYYNANAA--QTLGHP------PHMHQHGMAMGMGGGG 450
            :::..::.|.|...|..||        :..|..|:  |.|..|      ||...:.:.|| |.||
Mouse   148 TSIPPLMMPAPLVPSVSTS--------SLPNGTASLIQPLSIPYSSSTLPHASSYSLMMG-GFGG 203

  Fly   451 GGGHHIMNNNTMGRASSQSSRFSGSRSSHEIGRG-----YPPRNLYLE----LERERSCIEGSPP 506
            .......:...:|.:||.||..|.|.:|.:.|..     .|.|..|.:    |::..|.......
Mouse   204 ASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQ 268

  Fly   507 SDNVMFDNQCYATTPSSSNGNSDQDQSYGQQQS--------------SGQHPQQQQGPPQRSSRH 557
            :.|.:..:....|..::....:|.|.. ||.::              :|| |.....||:.....
Mouse   269 ARNALLQSNLSQTQLATIWTLADIDGD-GQLKAEEFILAMHLTDMAKAGQ-PLPLTLPPELVPPS 331

  Fly   558 QHHHQQAPNVTPTPGS--------PTSRLLLEYEMHLRNTLAKG-MDAESYSLHTFEALLSQSME 613
            ....:|..:|..|..|        |..:|.:.:|...:....:| |:.|...    :.|:.|...
Mouse   332 FRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRR----QVLMEQQQR 392

  Fly   614 NLE-FAESLPGSNQRSPYPLRRPNAKSS---TLPLPPHRPLSTIRDKERDRDRDGYYSDRNELIR 674
            ..| .|:......:|....|:....|..   ...|...|.|...|::||.::.:...:.:.||.|
Mouse   393 EAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELER 457

  Fly   675 ERERERDRGYLSDHNSSFSNSRCASCIGESARAQ--WFRHSDGWRSGSSTIGSGSG-HGMMTQQI 736
            :|..|.:|            .|....:.:..|.|  ..|.|...:|....:.:.:| |    |||
Mouse   458 QRRLEWER------------LRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKH----QQI 506

  Fly   737 PGSGHKRSPWDSLPSLRQDSSLNDSGYKSARADSLEQRAEFIRQDSLRSEY-LSDRESRYGIVQ- 799
            .|                            |...::     ||:.:.::|. :.|::....|::
Mouse   507 SG----------------------------RLQDVQ-----IRKQTQKTELEVLDKQCDLEIMEI 538

  Fly   800 ---QASIESTDSRMCYLTSSEISDDDRMSLTTAVSDEDDGESVMASPYKAKATGTAASSFNCTGA 861
               |..::...:::.||...:...::|:......:..|.|.|::......|
Mouse   539 KQLQQELKEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKKSSEK-------------- 589

  Fly   862 VRKAGFLSVKKWLLRKKHQIELARKRGWKGYWVCLKGTTLLFYPCDSREGRSVEAAPKHLIIVDG 926
                     ::...|.|.|::...|.           |.......||...:....:      :|.
Mouse   590 ---------EELCQRLKEQLDALEKE-----------TASKLSEMDSFNNQLKCGS------MDD 628

  Fly   927 AIMQPIPEHPKRDYIFCLSTAF------GDAYLFQAPCQVELENW---------------VNSIH 970
            .::|.:     ...:.||:..|      .::|..|   |:.||..               :..|.
Mouse   629 CVLQCL-----LSLLSCLNNLFLLLKELRESYNTQ---QLALEQLHKIKRDKLKEIERKRLEQIQ 685

  Fly   971 SACAAAFARHRGKTGTLHLLQEEIFRLEKAIESDHKLKHMAELQQSVVTDQETR--HQIQTQILQ 1033
            .......|..:.|.|..:|.:|.|.:     |.:.|.|.:.|.:....|.:|.|  ...|::...
Mouse   686 KKKLEDEAARKAKQGKENLWRESIRK-----EEEEKQKRLQEEKSQDKTQEEERKAEAKQSETAS 745

  Fly  1034 WEENLERLHCEQFRLRCYMASLQSGELPNPKSLLTHVSRPTKNTLNKLGVFTVSSFHA----FIC 1094
            ...|...|:..:.|....| |..||:          :.:..:.|:.:.| :...||..    |.|
Mouse   746 ALVNYRALYPFEARNHDEM-SFSSGD----------IIQVDEKTVGEPG-WLYGSFQGKFGWFPC 798

  Fly  1095 ARSPSLLNNLLAGRGATKRRPPMLSRSNSGSSRR--------------SMQMNSRDEPEKTFKVA 1145
            .....:|::..|........||.:|.|.:.:|.:              ::.:|:..:.:..|...
Mouse   799 NYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRT 863

  Fly  1146 MPDNAYSTVYLRDAMSVEEFLASA-CARRNLNPMEHFVRVKKRRDMEDHNYFVPHRNDLI----- 1204
            :...:.|.:: ....:||...|.| |:          ...||    |:|..|..|  |:|
Mouse   864 VSPGSVSPIH-GQGQAVENLKAQALCS----------WTAKK----ENHLNFSKH--DVITVLEQ 911

  Fly  1205 -ENY----LHNHE--FVEVCMKILYQVELQRTTLEQMW----------GFSVEAELIENAERQDE 1252
             ||:    :|...  |.:..:|::...|:||...|.::          .:.|.:......|....
Mouse   912 QENWWFGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIA 976

  Fly  1253 LCCYVSRVEDKSVAMHNGIIKGDEIMVI-------NGAIVSDLDMM---YLESVLQEEQSLSMMM 1307
            |..| |.||...:.    ..:|:||:|.       .|:|.....:.   |:....||....:...
Mouse   977 LYSY-SSVEPGDLT----FTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKS 1036

  Fly  1308 RSSRTEPPDLVGIMRVTD----DMIDSLVCPPPPTDPPVMSEEMITGLIVPAPGW-------NGT 1361
            .:|..:|.    |.:||.    ...:.|...|         .::|..|.....||       .|.
Mouse  1037 GASNKKPE----IAQVTSAYAASGTEQLSLAP---------GQLILILKKNTSGWWQGELQARGK 1088

  Fly  1362 SKD----------LYSPEAESSPAT--SFVDPAAMAAQLAVGGVGGVVVGGLGVAKPTSRTSSFE 1414
            .:.          |..|.:|.:..|  :.....||...:|..             :.....|..:
Mouse  1089 KRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANN-------------EDELNFSKGQ 1140

  Fly  1415 IENLLKTAEQETRKSSP----------TGSVTSSV--STTALTPSRQ----------LTDAEKLR 1457
            :.|::       .|..|          ||...|:.  .||...||:|          :...|:.|
Mouse  1141 LINVM-------NKDDPDWWQGETNGLTGLFPSNYVKMTTDSDPSQQWCADLQALDTMQPTERKR 1198

  Fly  1458 K-VVMELVDTERTYVKHLNNLLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFLQNLEESL 1521
            : .:.||:.||..|:..|..::|.:.:.|....||:.|::..:|.|..|::....:.|:.|...
Mouse  1199 QGYIHELIQTEERYMDDLQLVIEVFQKRMAESGFLTEADMALIFVNWKELIMSNTKLLRALRVR- 1262

  Fly  1522 DLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVL-HPNEGNHALQEFL- 1584
                     :..|..:..:..||......::|.:.|..||:.......:| ...:.:...:|||
Mouse  1263 ---------KKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGATLLQQKTDEDTDFKEFLK 1318

  Fly  1585 -AARNPKQQHSSTLESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINE 1648
             .|.:|:.: ...|.|:|:||:|||.:||||::.:...|.....:|..|..||:..|::...:||
Mouse  1319 KLASDPRCK-GMPLSSFLLKPMQRITRYPLLIRSILENTPQSHVDHSSLKLALERAEELCSQVNE 1382

  Fly  1649 MQR------------IHEEYGAIFDHLFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKIK 1701
            ..|            .|.:...:.:.|......:|     |.|..||:.|.         |.|.|
Mouse  1383 GVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNC-----LGPRKLLHSGK---------LYKTK 1433

  Fly  1702 KGLELHAMCFVFKSAVVFLCKE--RLRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSA 1764
            ...||||..|.....:.:|.::  .....:||.     |:.:..:...|:..|.:.||.|:..:.
Mouse  1434 SNKELHAFLFNDFLLLTYLVRQFAAASGHEKLF-----NSKSSAQFRMYKTPIFLNEVLVKLPTD 1493

  Fly  1765 KDMDSHFLWELIHLRSQLQRRSEKVYVLSNSTADFRNAFLKTIR 1808
            ...|.. ::.:.|:        ::||.|.....:.|.|:::.|:
Mouse  1494 PSSDEP-VFHISHI--------DRVYTLRTDNINERTAWVQKIK 1528

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	20/145 (14%)
		PH	873..974	CDD:278594	18/121 (15%)
		TIAM1_RBD	1141..1219	CDD:176413	19/90 (21%)
		PDZ_signaling	1221..1308	CDD:238492	20/106 (19%)
		RhoGEF	1456..1647	CDD:238091	48/194 (25%)
		PH2_Tiam1_2	1650..1823	CDD:269957	34/173 (20%)
		PH	1693..1812	CDD:278594	25/118 (21%)
Itsn2	NP_001185897.1	EH	15..107	CDD:197477
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	220..242		7/21 (33%)
		EH	238..317	CDD:197477	11/79 (14%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	335..382		10/46 (22%)
		Smc	350..>747	CDD:224117	83/502 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	716..742		7/25 (28%)
		SH3_Intersectin2_1	747..803	CDD:212921	13/67 (19%)
		INTAP	806..883	CDD:374674	11/77 (14%)
		SH3_Intersectin2_2	883..934	CDD:212923	15/66 (23%)
		SH3_Intersectin2_3	973..1024	CDD:212925	12/55 (22%)
		SH3_Intersectin2_4	1045..1103	CDD:212927	10/66 (15%)
		SH3_Intersectin2_5	1118..1171	CDD:212929	10/72 (14%)
		RhoGEF	1202..1381	CDD:366202	47/189 (25%)
		PH_13	1400..1554	CDD:374702	32/157 (20%)
		C2_Intersectin	1543..1677	CDD:176021
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.910