DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and uig-1

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_001256489.1	Gene:	uig-1 / 179808	WormBaseID:	WBGene00009337	Length:	972	Species:	Caenorhabditis elegans

Alignment Length:	1022	Identity:	208/1022 - (20%)
Similarity:	353/1022 - (34%)	Gaps:	292/1022 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly  1296 VLQEEQSLSMMMRSSRTEPPDLVGIM---RVTDDMIDSLVCPPPPTDPPVMS----------EEM 1347
            :|.:.:|..::.:.|.....:..|.:   :.:..:|:.|   |....|||:.          :|.
Worm   119 ILDQNESKYILQQQSAAAIEEKKGTLLRRKSSKKLIEPL---PMIITPPVLMARTQCCAVCYKEK 180

  Fly  1348 ITGLIVPAPGWNGTSKDLYSPEAESSPAT-----------SFVDPAAMAAQLAVGGVGGVVVGGL 1401
            .||..|      .:....|..||.||..|           |||..:.                |.
Worm   181 STGYSV------SSRTQSYPLEAMSSVTTDRSTEEETARLSFVSSST----------------GY 223

  Fly  1402 GVAKPTSRTSSFEIENLLKTAEQETRKSSPTGSVT--SSVSTTALTPSRQLTDAEKLRKVVMELV 1464
            ..|:.:.|:|....|:...:|.......|.|.|::  ||...:||.....||   .|.::.:||:
Worm   224 SSARSSLRSSDGGDESNNNSAGNRDSAISNTFSLSSQSSARRSALLTKGSLT---HLDRIAIELL 285

  Fly  1465 DTERTYVKHLNNLLEHYLEPM--KRETFLSNAE-INALFGNIHEIVTFQRQFLQNLEESLDLEPD 1526
            |||||||:.||.:::.|::.:  .||......: |::|||.|..|..|.:| |.|..::.||  |
Worm   286 DTERTYVEDLNAVIKGYMDFLVEDREKLKVTLDAISSLFGCIERIFAFNKQ-LYNQLDAADL--D 347

  Fly  1527 FNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPKQ 1591
            ..|...|             |:.....|:.|..:|.::.:....|:     |..|:.|.||..::
Worm   348 CVKMSRC-------------FVESSGKFEDYIEYCTNYHRMMSTLY-----HLQQQPLVARALQE 394

  Fly  1592 Q-----HSSTLESYLIKPIQRILKYPLLLQQ-MRNL-TDTRADEHVHLCEALKGMEKVAEHINEM 1649
            :     ||..|.:||:||:||||||.|.|:. ::|: :.|..:|.:.:..|.:.|...|..||:.
Worm   395 RQLALNHSLQLSAYLLKPVQRILKYHLFLENILKNMPSTTHPEELIQVKRAHEVMTSQAARINDE 459

  Fly  1650 QRIHEEYGAIFDHLFRQHQKSCKQPIDLSP----GDLLYYGGVEWLNISDFLGKIKKGLELHAMC 1710
            ::..|....: ..|....||.....|.:|.    ||||......           :.|.:...:.
Worm   460 KKKAEHIERV-GQLQSTLQKWKADEIQISNLSAYGDLLLEATFR-----------QAGSKTTRLL 512

  Fly  1711 FVFKSAVV--------FLCKERLRQKKKLMGVSSKNATNE----VEIIRYQVLI---PVTEVQVR 1760
            |:|:..::        ::||:.:        :.|....||    .|.:.:|||.   |..:....
Worm   513 FLFEEMLLIVKQRGANYVCKDYI--------MCSNLMLNEWICPEEPLSFQVLSFDNPRAQYVFM 569

  Fly  1761 ASSAKD-----------MDSHFLWELIHLRSQLQRRSEKVYVLSNSTADFRNAFLKTIRQIIR-- 1812
            |||.:.           |..|:..|:.....||....:...::.....:|....:|..:::.:
Worm   570 ASSMEQKRTWMQELKRMMLDHYSVEIPEKTKQLMLSIDNTRIVPFGRPEFAEVSMKNHKKVPKYL 634

  Fly  1813 -----------ESVRNMSIPMKN-FGGSSGSVSG----------HSSQGMGSMGYPGNSQTLERP 1855
                       ||.|..|:.... .|||..|:|.          |.:.|:.:...|..|:::
Worm   635 EKRRKSVDAKEESNRRRSLSASRLLGGSKTSISEPIKEEQKCTCHMNGGIYNGANPTTSKSI--- 696

  Fly  1856 KQQITIVHGSHTLGKPKKKSGSQRHSAGNIDYDNLSGSQEADDLPPSVGVVHYASGHTHGQQMQP 1920
                 |.|.|.|                   |.....:|..|.        :|.         :|
Worm   697 -----ISHPSLT-------------------YQQSHPTQSTDP--------NYC---------RP 720

  Fly  1921 AGFRGRSKTVGDVTEITCSSPEPHQQQQQQQQQQQQLMQQGHAHAHPHPHPHPREPPPPPIRQPH 1985
            ...|.:|....|:.::..:.|........:.:.||               ...|..||
Worm   721 PVCRTQSAATIDLNQVHITEPNSAAVMSSRSRYQQ---------------ARNRRLPP------- 763

  Fly  1986 LHHHSSDIERIDPGTKSEGEED-------------SQQGTIRPKATLGRTP---NHLTLSTTSTL 2034
              .|:.  |.....::..|||:             |...:.:|..|..|:.   :....||.:|:
Worm   764 --RHNQ--ESRSTSSRRMGEEEIDRTFDQLYEELVSFGDSTKPTVTSNRSQRLRSEAPQSTVTTI 824

  Fly  2035 SVGSTGSQARLIQSSHPPASYQPVLMKDLGKPSPDESELESESEVHAAQVAASFNLSLGSSGSLF 2099
            :|.:|.:.|.|                                     .:|...|..|.|. ||.
Worm   825 TVAATPATATL-------------------------------------SIAECANTRLRSK-SLT 851

  Fly  2100 ASDARDNQTQSRTQSQIHSLHPSPRQSPRQSPRQSPKQDIEVIEIFESEAERLAASQQVAVVHQH 2164
            ..|..:.:..|...| |...:....|..::|.:....|:.|..::......|.:.:....::|
Worm   852 RLDEYEAEPISLKPS-IERRYSKVDQLKKRSRKYQVNQEPEAADVLRPSVGRFSLTDHEIIIH-- 913

  Fly  2165 PSDQARESGHKQRRDRDRNPTETDATARFMDKHLSDLEQENLTDGTC 2211
                 .|..:|:...|.|.|.....||. :|.:.......|....||
Worm   914 -----GEEPYKRSSGRQRTPLTAQQTAE-LDDYFPSSNSMNSAPQTC 954

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413
		PDZ_signaling	1221..1308	CDD:238492	2/11 (18%)
		RhoGEF	1456..1647	CDD:238091	61/200 (31%)
		PH2_Tiam1_2	1650..1823	CDD:269957	37/215 (17%)
		PH	1693..1812	CDD:278594	23/144 (16%)
uig-1	NP_001256489.1	RhoGEF	280..458	CDD:214619	61/198 (31%)
uig-1	NP_001256489.1	PH_PLEKHG1_G2_G3	439..590	CDD:270063	32/170 (19%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1291
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.940