DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Ect2

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_031926.2	Gene:	Ect2 / 13605	MGIID:	95281	Length:	913	Species:	Mus musculus

Alignment Length:	923	Identity:	181/923 - (19%)
Similarity:	326/923 - (35%)	Gaps:	247/923 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly  1131 QMNSRDEP--EKTFKVA-MPDNAYSTVYLRDAMSVEEFLASACARRN---------------LNP 1177
            :..|.|.|  |..|.|. ..::.::.:|..|...|...:...||:|.               ||.
Mouse   115 EFESLDSPEFENIFVVTDFQNSVFNDLYKADCRIVGPPVILNCAQRGEPLPFSCRPLYCTSMLNL 179

  Fly  1178 MEHFVRVKKRRDMEDHNYFVPHRNDLIENYLHNH--EFVEVCM---KILYQVELQRTTLEQMWGF 1237
            :..|...:|:.::......|.|...:|....::.  ..|..|.   |....|.|....::..|
Mouse   180 VLCFTGFRKKEELVKLVTLVHHMGGVIRKECNSKVTHLVANCTQGEKFRVAVSLGTPIMKPEW-- 242

  Fly  1238 SVEAELIENAERQDELCCYVSRVEDKSVAMHNGIIKGDEIMVINGAIVSDLDMMYLESVLQEEQS 1302
                 :.:..||::|. |:.:.|:|..          :|..|   ....|..:.:|....:|:.|
Mouse   243 -----IYKAWERRNEQ-CFCAAVDDFR----------NEFKV---PPFQDCILSFLGFSDEEKHS 288

  Fly  1303 LSMM--MRSSRTEPPDLVGIMRVTDDMIDSLVCPPPPTDPP----VMSEEMITGLIVPAPGWNGT 1361
            :..|  |:.....|   ||..|.|..:::.......|.:|.    |:.:|...|.| ......|.
Mouse   289 MEEMTEMQGGSYLP---VGDERCTHLIVEENTVKDLPFEPSKKLFVVKQEWFWGSI-QMDARAGE 349

  Fly  1362 SKDLY----SPEAESSPATSFVDPAAMAAQLAVGGVGGVVVGGLGVAKPTSRTSSFEIENLLKTA 1422
            :..||    :||.:.|                        |..|.::.|.|......::..|...
Mouse   350 TMYLYEKANTPELKKS------------------------VSLLSLSTPNSNRKRRRLKETLAQL 390

  Fly  1423 EQET-------RK---------------------------SSPTGSVTSSVSTTALTPSRQLTDA 1453
            .:||       ||                           .:|.....||.|:|.:.|.:...
Mouse   391 SRETDLSPFPPRKRPSAEHSLSIGSLLDISNTPESSIHYGETPKSCAKSSRSSTPVPPKQSAR-- 453

  Fly  1454 EKLRKVVMELVDTERTYVKHLNNLLEHYLEPMKRE-----TFLSNAEINALFGNIHEIVTFQRQF 1513
               .:|..||..||..||..|..:::.:..|::.|     ..|:..||..:||:|.:|.....:.
Mouse   454 ---WQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGGPILAPEEIKTIFGSIPDIFDVHMKI 515

  Fly  1514 LQNLEESLDLEPDFNKFEHCGQFRNVLFAIGSAFLYY-----------VNHFKLYSSFCASHSKA 1567
            ..:||   ||..::::..          :||..||.|           ||.|::.........|.
Mouse   516 KDDLE---DLIANWDESR----------SIGDIFLKYAKDLVKTYPPFVNFFEMSKEMIIKCEKQ 567

  Fly  1568 QKVLHPNEGNHALQEFLAARNPKQQ-HSSTLESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVH 1631
            :...|         .||.....|.: ...:|...||:|:||:....|||..::..|.....:...
Mouse   568 KPRFH---------AFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKST 623

  Fly  1632 LCEALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQPIDLSPGDLL-----YYGGVEWL 1691
            |.:|:..:::|..||||.:|..|....|||.::.         :|..|.:||     ....||.:
Mouse   624 LEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYE---------VDGCPANLLSSHRSLVQRVETV 679

  Fly  1692 NISDFLGKIKKGLELHAMCFVFKSAVVFL---CKERLRQKKKLMGV--SSKNATNEVEIIRYQVL 1751
            ::.:           |. |...:...:||   |.|..|::.|::|.  |..:.|.....:::..|
Mouse   680 SLGE-----------HP-CDRGEQVTLFLFNDCLEIARKRHKVIGTFRSPHDRTRPPASLKHIHL 732

  Fly  1752 IPVTEVQVRASSAKDMDSHFLWELI--------HLRSQLQRRSEKVYVLSNSTADFRNAFLK--- 1805
            :|:::::......:..|.|..:.|:        ::....|..||::.         :.::||
Mouse   733 MPLSQIKKVLDIRETEDCHNAFALLVRPPTEQANVLLSFQMTSEELP---------KESWLKMLC 788

  Fly  1806 -----TIRQIIRESVRNMSIPMKNFGGSSGSVSGHSSQGMGSMGYPGNSQTLER-------PKQQ 1858
                 ||.:...|::..::.| ::|..::..:.  |:....|......|:.:.|       ||:.
Mouse   789 RHVANTICKADAENLMYVADP-ESFEVNTKDMD--STLSRASRAIKKTSKKVTRAFSFSKTPKRA 850

  Fly  1859 ITI-VHGSHTLGKPKKKSGSQRHSAGNIDYDNLSGSQEADDLP-PSVGVVHYASGHTHGQQMQPA 1921
            :.: :..||:      ..|....|:|.:....||.:.....:| ||:..:             |:
Mouse   851 LRMALSSSHS------SEGRSPPSSGKLAVSRLSSTSSLAGIPSPSLVSL-------------PS 896

  Fly  1922 GFRGRSKTVGDVT 1934
            .|..||.|:...|
Mouse   897 FFERRSHTLSRST 909

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413	17/98 (17%)
		PDZ_signaling	1221..1308	CDD:238492	16/88 (18%)
		RhoGEF	1456..1647	CDD:238091	48/207 (23%)
		PH2_Tiam1_2	1650..1823	CDD:269957	36/198 (18%)
		PH	1693..1812	CDD:278594	23/139 (17%)
Ect2	NP_031926.2	PRK09630	<71..>125	CDD:170022	3/9 (33%)
		BRCT_Ect2_rpt1	176..251	CDD:349365	15/81 (19%)
		BRCT_Ect2_rpt2	270..349	CDD:349364	17/82 (21%)
		RhoGEF	456..640	CDD:214619	49/205 (24%)
		PH_Ect2	631..804	CDD:269936	40/202 (20%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.910