DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment sif and AgaP_AGAP006514

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_316542.4	Gene:	AgaP_AGAP006514 / 1277109	VectorBaseID:	AGAP006514	Length:	1746	Species:	Anopheles gambiae

Alignment Length:	1706	Identity:	324/1706 - (18%)
Similarity:	527/1706 - (30%)	Gaps:	577/1706 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly   278 SGVNTNTPGVIVTGVGNVG-SDMCGQNHVGSQVGNDDPAACQMDMDLSKSEGTQAGGGLHQSV-- 339
            ||.....|..|.|..|.|. :...|:....:.:||.:|...........::..::|.|..:||
Mosquito   177 SGATYLAPPPIRTSAGTVKLASTYGKKSRPTILGNGNPDPYDFMRRSRLTQPAESGAGGPKSVKS 241

  Fly   340 -------GTCTSSSKGTGTRNKDFGDDMTRDAHSHDMHQHNVINNNTR---RKTKSTEDMNV--- 391
                   |...|:|...|......|...:..|.|   ...::..|.:|   ||.:|.:|..|
Mosquito   242 SLKEKLRGRAKSTSPSRGRSGSTAGGKRSSAARS---PSPSLSKNGSRVGGRKLESFQDCWVGEP 303

  Fly   392 --DTSTLKRMLKPMPSTESPVTSPEMGRRRYNYYNANAAQTLGHPPHMHQHGMAMGMGGGGGGGH 454
              ||..|         .:|| ||....|:.....:.|....:.          ..|:|||.|||.
Mosquito   304 DRDTDLL---------DDSP-TSTAQNRKSILRCDVNPYDLIS----------LSGIGGGSGGGS 348

  Fly   455 HIMNNNTMGRASSQSSRFSGSRSSHE-------IGRGYP-PRNLYLELERER--------SCIEG 503
            .  .||.:...:.....|......:|       ..|..| |.|....:..:|        :.:.|
Mosquito   349 G--GNNMLNEFAPDGDEFDLHSQKYENILDTLYANRYNPNPANSIAAINGQRIKLFDDSGTSVSG 411

  Fly   504 SPPSDNVMFDNQCY--ATTPSSSNGNSDQDQSYGQQQSSGQHPQQQQGPPQRSSRHQHHHQQA-- 564
            :|..|:|  |...|  ...|......:|:..|            ...|.|:|..|.:....:|
Mosquito   412 APVYDDV--DEFVYDPVEVPVEEAAATDELSS------------PSAGTPERPPRSKQRQLEATK 462

  Fly   565 ---------------------PNVTPT--------PGS-----PTSRLLLEYEMHLRNTLAKGMD 595
                                 |.|||:        |.|     |:|.:.:|     ::|.|.|..
Mosquito   463 ENDDSRSLPSTAPSGTAEAGSPGVTPSAIKSILKRPSSMPAAPPSSMVTVE-----KDTGAGGPG 522

  Fly   596 AESYSL-HTFEALLSQSMENLEFAESLPGSNQRSPYPL--------RRPNAKSSTLPLP------ 645
            .::..| ||....|::...|...:.|..|::....:..        :|.:.....||.|
Mosquito   523 YDTIRLNHTLVQRLAKLTTNANGSASEEGASTFGKHGTISARHLTDKRNSGSQFYLPTPFPVIAS 587

  Fly   646 ---PHRPLSTIRDKERDRDRDGYYSDRNELIRERER--------ERDRGYLSDHNSSFSNSRCAS 699
               |....||...:::     .::...||:|.:.:|        |...|.::......:|
Mosquito   588 TSEPDATQSTTGGRKK-----VHFLVENEIIHDDDRLFAQMLFTEVVPGEVTPMPVKTTN----- 642

  Fly   700 CIGESARAQWFRHSDGWRSGSSTIGSGSGHG------------MMTQQIPGSGHKRSPWDSLPSL 752
              |.:.|......|.|.|..:.......|..            ..|.|:|...:.::|..:.||.
Mosquito   643 --GATERGAAAEESIGSRKDTPASSKQGGKARPKLPPPPPPPPTTTTQLPTGANGKAPASATPSP 705

  Fly   753 RQDSSLNDSGYKSARADSLEQRAEFIRQDSLRSEYLSDRESRYGIVQQASIESTDSRMCYLTSSE 817
            ...:...|                |.                :|::......|:|      .||.
Mosquito   706 PGSNVYED----------------FC----------------FGLMAGVRRSSSD------RSSS 732

  Fly   818 ISDDDRMSLTTAVSDEDDGESVMASPYKAKATGTAASSFNCTGAVRKAGFLSVKKWLLRKKHQIE 882
            .|...|..:....|......|..:||....:.|::.|....:|.:.:          |..:|..|
Mosquito   733 TSTSSRTVVQVLASSSSFSSSSSSSPSSPSSCGSSPSPIASSGGIHR----------LEIRHDPE 787

  Fly   883 LARKRGWKGYWVCLKGTTLLFYPCDSREGRSVEAAPKHLIIVDGAIMQPIPEHPKRDYIFCLSTA 947
            ...:...|...:.:.|.     .|.|            .:.||||...|         |:..|..
Mosquito   788 PEEEHYQKKTSILITGD-----DCYS------------TVNVDGASDTP---------IYQSSVV 826

  Fly   948 FGDAYLFQAPCQVELENWVNSI-------HSACAAAFARHRGKTGTL-----H-------LLQEE 993
            ..|..:..:|.....:...|:|       .|...:..::.|..||.|     |       :.:||
Mosquito   827 VNDGSVVASPTNEIRQTASNTITISVSSPTSTLESRGSKGRMATGKLAGDEQHANGTVVTMGREE 891

  Fly   994 IFRLEKAIESDHKLKHMAELQQSVVTDQETRHQIQTQILQWEENLE------------------- 1039
            :..:.....       :..:..|.||....|..|.....|.|:::|
Mosquito   892 VTTVSTGTS-------VIPIGGSSVTGSNGRTLINLDFGQPEQSVESSTAAQTDTKTSEDVTPIA 949

  Fly  1040 ------------------RLHCEQFRLRCYMASLQSGELPNPKSLLTHVSRPTKNTLNKLGVFTV 1086
                              ..|.:|.::....||:..  .|.|..|...:...|:.|.      ||
Mosquito   950 PVPSDSNDSKDSPKVREDAAHDQQQQIVVQQASVDL--TPTPVVLRRSLKPTTEPTT------TV 1006

  Fly  1087 SSFHAFICARSPSLLNNLLAGRGATKRRPPMLSRSNSG------------------SSRRSMQMN 1133
            :|..|.:|..  |.:..||        ..|.||:.:.|                  .||..:.|:
Mosquito  1007 NSLQAKVCRN--SFIAKLL--------EDPSLSQLSEGLDYELIAKLIESSLLRLKESRNGLDMS 1061

  Fly  1134 -SRDE--------------PEKTFKVAMPDNAYSTVYLRDAMSVEEFLASACARRNLNPMEHFVR 1183
             ::||              .|:..|:..|.|.                ||...::|..
Mosquito  1062 GTKDEDDVSKMIEQSLLKIQEERSKLTAPSNP----------------ASQSPQQNSG------- 1103

  Fly  1184 VKKRRDMEDHNYFVPHRNDLIENYLHNHEFVEVCMKILYQVELQRTTLEQMWGFSVEAELIENAE 1248
             .||..:...|                                       .:|.:...||:|..:
Mosquito  1104 -SKRSSVSSGN---------------------------------------SYGSAAAYELMEFDQ 1128

  Fly  1249 RQDELCCYVSRVEDKSV--AMHNGIIKGDEIMV--------INGAIVSDLDMMYLESVLQEEQSL 1303
            ..:...||.|...|.:|  ..|:...|..:::|        ||.|...|.|..:.||:
Mosquito  1129 LGELSDCYQSCSSDLTVDDDAHSSRSKFYQMLVDATLSELEINAANEDDDDEHHYESI------- 1186

  Fly  1304 SMMMRSSRTEPPDLVGIMRVTDDMI-DSLVCPPPP---TDPPV-----------------MSEEM 1347
                              |:..|.| :.:...|||   |.||:                 .|:..
Mosquito  1187 ------------------RLNGDPIYEEINDIPPPLPLTAPPIDDVDLEKQRNARSIFEGASKYD 1233

  Fly  1348 ITGLIVPAP-----------GWN-GTSKDLYSPEAESSPAT------------------------ 1376
            |...:|.|.           |:. |.:.|:...|.||.|.|
Mosquito  1234 ILSYLVDAKERGIVQEDGSYGFQFGGAGDIILEEEESEPITELRHHQRQISDISSRLSHLSGISD 1298

  Fly  1377 SFVDPAAMAAQLAVG----GVGGVVVGGLGVAKPTSRTSSFEIENLLKTAEQETRKSSPTGSVTS 1437
            |..|.:..|..|:.|    ....:.....||...||:||....:|.:.|.         |.|.|:
Mosquito  1299 SSEDTSLSAGTLSNGRSSKASAEIERNDSGVGSETSKTSRSRWQNPISTT---------TTSTTT 1354

  Fly  1438 SVSTTALTP-----------SRQLTD-----------------AEKLRKVVMELVDTERTYVKHL 1474
            |...|.:||           ..|:|:                 ||: ::::.|:|:||..|.:.|
Mosquito  1355 SSLLTKITPIHLCEDCDGPVETQVTESGVMYAPLVCRKCGKKRAER-KEIITEIVETEEKYGRDL 1418

  Fly  1475 NNLLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFL-QNLEESLDLEPDFNKFEHCGQFRN 1538
            ..:||.:.:||.....|:..:::|:|.|:.|::. ..||| :.:.::||:.      ...|....
Mosquito  1419 QIILEEFYQPMLVAGLLNQEQLSAIFLNVEELLE-NNQFLAERMRDALDIA------NEQGDDDL 1476

  Fly  1539 VLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVL-HPNEGNHALQEFLAARNPKQQ--HSSTLESY 1600
            :..:||..||........:.::|.....|..:| :..:....|:.||.....:..  ....|.|:
Mosquito  1477 LTVSIGKIFLEAAPMLHAFENYCVRQGAASLLLANLEKEKELLRIFLRVSQMENAVLRRMNLNSF 1541

  Fly  1601 LIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALK-GMEKVAEHINEMQRIHEEYGAIFDHLF 1664
            |:.|:||:.||||||.::..:|..    |:...|.|| ..||:..|:|.|.|   |...:...|:
Mosquito  1542 LMVPVQRVTKYPLLLARLYKVTPA----HLEGKELLKQSQEKIELHLNHMNR---EAKDVPTKLW 1599

  Fly  1665 RQHQKSCKQPIDLSPG 1680
            |:...|       |||
Mosquito  1600 RRISSS-------SPG 1608

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	21/131 (16%)
		PH	873..974	CDD:278594	19/107 (18%)
		TIAM1_RBD	1141..1219	CDD:176413	9/77 (12%)
		PDZ_signaling	1221..1308	CDD:238492	19/96 (20%)
		RhoGEF	1456..1647	CDD:238091	51/195 (26%)
		PH2_Tiam1_2	1650..1823	CDD:269957	8/31 (26%)
		PH	1693..1812	CDD:278594
AgaP_AGAP006514	XP_316542.4	RhoGEF	1403..1588	CDD:279015	52/195 (27%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910