DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment sif and AgaP_AGAP006382

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_316415.4	Gene:	AgaP_AGAP006382 / 1276993	VectorBaseID:	AGAP006382	Length:	1069	Species:	Anopheles gambiae

Alignment Length:	1215	Identity:	267/1215 - (21%)
Similarity:	405/1215 - (33%)	Gaps:	440/1215 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly  1054 SLQSGELPNPKSLLTHVSRPTKNTLNKLGVFTVSSF-------HAFICA--RSPSLLNNL----- 1104
            |.|..|.||  .|.|.||........|    |.|.|       |....|  .:|:....|
Mosquito   122 SRQDAESPN--HLSTTVSESVLQKFRK----TFSHFKTSKSAPHPVTAAGVTTPTTTTGLTTSTD 180

  Fly  1105 LAGRGAT--------------KRRPPMLSRSNSGSSRRSMQMNSRDE---PEKTFKVAMPDNAYS 1152
            |...|||              .|..|::.||:  ..||..:.:.||:   .:...:|.:.::|..
Mosquito   181 LQTAGATMSVDDKQQQQQHHHHRFGPLIWRSS--KERRKTKSHRRDKCNSGDSGIQVELDNDADQ 243

  Fly  1153 TVYLRDAMSVEEFLASACARRNLNPMEHF--VRVKKRRDMEDHNYFVPHR--------------- 1200
            .:.|                :.|:|:||.  ..|...|:.......||.|
Mosquito   244 HLLL----------------QQLHPLEHHHPAGVDAGRNGYTATGGVPVRRANSAKVSTASSTGS 292

  Fly  1201 --NDLIENYLHNHEFVEVCMKILYQVELQRTTLEQMWGFSVEAELIENAERQDELCCYVSRVEDK 1263
              :.|:::.|                             |::.:..||...       ::|:..|
Mosquito   293 GSSTLLKHKL-----------------------------SLKGKGKENVSN-------ITRLSGK 321

  Fly  1264 SVAMHNGI-----IKGDEIMVINGAI-VSDLDMMYLESVLQEEQSLSMMMRSSRTEPPDLVGIM- 1321
            |::..:|:     ..||......||: |.|..::..|.|..|      ::.|.|...|..:|:.
Mosquito   322 SLSQPSGLDCIVASGGDSADCGAGAVAVEDELLVAAEPVFAE------VLFSFRPGGPQELGLEK 380

  Fly  1322 -----------------RVTDDMI--DSLVCPPP---------PTD-----PPVMSEEMITGLIV 1353
                             |:..|.|  :...|..|         |.|     |.....:.|  :|:
Mosquito   381 GALVEVLKREQGPWWWGRLKSDAIVSEGADCSKPSNGGACGWFPKDFVKIVPTYAKPKQI--III 443

  Fly  1354 PAPGWNGTSKDLYSPEAESSPATSFVDPAAMAAQLAVGG--------VGGVVVGGLGVAKPTSRT 1410
            .:.|.....:.|:.|..|.....|     :.|:.|..||        :||:.:.|...|.|....
Mosquito   444 SSQGEANKQQQLHHPAGEDDDGAS-----SSASTLIEGGSHCDSTRLMGGLSMAGDATAAPVEEA 503

  Fly  1411 SSFEIENLLKTAEQETRKSSPTGSVTSSVSTTALTPSRQLTDAEKLRKVVMELVDTERTYVKHLN 1475
            ...| |....|:...|:::                             ::.||::||..|||.||
Mosquito   504 GQGE-EGAGATSHDLTKEN-----------------------------IIKELLETEINYVKLLN 538

  Fly  1476 NLLEHYLEPMK-RETFLSNAEINALFGNIHEIVTFQRQFLQNLEESLDLEPDFNKFEHCGQFRNV 1539
            :|...:::|:: ||...|...:|.:|.|:.:|..||:.||..|..::   |: |:
Mosquito   539 SLCLGFIKPLREREDVFSVESVNLIFSNLEKIWRFQQTFLDALRVAV---PN-NR---------- 589

  Fly  1540 LFAIGSAFLYYVNHFKLYSSFCASHSKA----QKVLHPNEGNHALQEFLAARNPKQQHSSTLESY 1600
               ||..||.|.:.|.:|||:|.|:.:|    :...:..|.|..|:....|.|..:   ..|.::
Mosquito   590 ---IGEVFLEYQSAFMVYSSYCNSYPRALMELENYANNKEANQILENCRMAENLPE---LPLSAH 648

  Fly  1601 LIKPIQRILKYPLLLQQM--RNLTD------------TRADEHVHLCE-----ALKGMEKVAEHI 1646
            |:.|||||.:|||.|.::  .:||.            |:::.....|.     ||..|.:|.|.:
Mosquito   649 LLAPIQRICRYPLHLSELVKHSLTRKELLPTLNLRKCTKSELETMDCREVFELALSAMRRVTEMV 713

  Fly  1647 NEMQRIHEEY----GAIFDH------------LFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISD 1695
            ||.:| |.||    .|.|::            ||.|     ...|.:||.        .|.|...
Mosquito   714 NEGKR-HSEYLSRIQARFENFQGPSINVHSTRLFLQ-----TDAIRMSPN--------LWNNTYT 764

  Fly  1696 F------LGKIKKGLELHAMCFVFKSAVVFL--CKERLRQKKKLMGVSSKNATNEVEIIRYQVLI 1752
            .      |...||.| |....:::|.. :||  |:.......|:.||:.|||      :|..
Mosquito   765 LFLFDRQLVYCKKDL-LKRTNYIYKGR-IFLDNCRILNLPDGKMFGVTLKNA------LRLY--- 818

  Fly  1753 PVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNSTADFRNAFLKTIRQIIRESVRN 1817
                          .|:...|.....||            |:|...|.|. |.:.||...||:..
Mosquito   819 --------------CDTRNKWFDFCFRS------------SSSKLRFLNT-LSSERQFCGESLFV 856

  Fly  1818 MSIPMKNFGGSSGSVSGHSSQGMGSMGYPGNSQTLERPKQQITIVHGSHTLGKPKKKSGSQRHSA 1882
            ..:.    ||::....              :....:|..||..            |:|.|.|.||
Mosquito   857 SELD----GGAASCFD--------------DDNLSDRDSQQYA------------KESLSARASA 891

  Fly  1883 GNIDYDNLSGSQEADDLPPSVGVVHYASGHTHGQQMQPAGFRGRSKTVGDVTEITCSSPEPHQQQ 1947
                    :|:...  :||:  ..|..||                   |..|....|...|.:..
Mosquito   892 --------TGAPPM--MPPA--APHSGSG-------------------GGNTLPKKSRKMPKEAV 925

  Fly  1948 QQQQQQQQQLMQQGHAHAHPHPHPHPREPPPPPIRQPHLHHHSSDIERIDPG-----TKSEGEED 2007
            .||||||||::..|                         .:.|:.:.|...|     .||.....
Mosquito   926 LQQQQQQQQVLASG-------------------------EYGSNSLGRRKLGNWFRKAKSTNSTP 965

  Fly  2008 SQQGTIRPK----ATLGRTPNHLTLSTTSTLSVG----------STGSQARLIQSSHPPASYQPV 2058
            ||..|..|.    |::|...||...|:.|:|:.|          .|||.|  :.||....|..|
Mosquito   966 SQSPTHHPMAMSLASIGNNNNHSDSSSQSSLASGFQQQRTLSTLGTGSTA--LTSSGTALSTGP- 1027

  Fly  2059 LMKDLGKPS-PD-------ESELESESEVHAAQVAASFNLSLGSS 2095
              ...|||| ||       ||. :|:|.......||:...:..||
Mosquito  1028 --GGKGKPSKPDTVAPVAVESS-QSQSITLTNATAATSTAAAASS 1069

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413	14/96 (15%)
		PDZ_signaling	1221..1308	CDD:238492	16/92 (17%)
		RhoGEF	1456..1647	CDD:238091	62/214 (29%)
		PH2_Tiam1_2	1650..1823	CDD:269957	43/196 (22%)
		PH	1693..1812	CDD:278594	27/126 (21%)
AgaP_AGAP006382	XP_316415.4	SH3	358..429	CDD:214620	14/76 (18%)
		RhoGEF	522..715	CDD:279015	62/212 (29%)
		PH_Collybistin_ASEF	720..853	CDD:269931	39/183 (21%)
		PH	759..845	CDD:278594	27/123 (22%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3519
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810