DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and AgaP_AGAP007712

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_308164.4	Gene:	AgaP_AGAP007712 / 1269511	VectorBaseID:	AGAP007712	Length:	1376	Species:	Anopheles gambiae

Alignment Length:	1595	Identity:	293/1595 - (18%)
Similarity:	514/1595 - (32%)	Gaps:	488/1595 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly   958 CQVELENWVNSIHSACAAAF---ARHRGKTGTLHLLQEEIFRLEKAIESDHKLKHMAELQQSVVT 1019
            |..::||: |.:.:..|.:.   ||..|...:            .||.::|        ...|..
Mosquito    34 CPQDIENY-NDLKTTGAGSVIGSARTNGSHNS------------DAITNNH--------SNMVPI 77

  Fly  1020 DQETRHQIQTQILQWEENLERLHCEQFRLRCYMASLQSGELPNPKSLLTHVSRPTKNTLNKLG-- 1082
            ...|.|....:....|:.|.:|.|    ||..:|.:::..|.|             ..|..:|
Mosquito    78 ISVTPHSPGAKFNFLEDTLNQLQC----LRESVAHMKNTTLQN-------------TALGGIGST 125

  Fly  1083 --VFTVSSFHAF-ICARSPSLL----------NNLLAGRGATKRRP----PMLSRSNSGSSRRSM 1130
              .|.|||...| .|...|.|.          :::|.|....:|:.    ..|:.... ||.:|:
Mosquito   126 VCSFVVSSTKLFSSCPSLPDLTIANPINVWPHHHVLYGLNNDRRKSWTAIEDLTECTK-SSHKSV 189

  Fly  1131 QMNSRD-EPEKTFKVA---------MPDNAYSTVYLRDAMSVEEFLASACARRNLNPMEHFVRVK 1185
            .::|.| |.:::.:.|         ......||..|.:|....:| ....|:|||.|:...:.::
Mosquito   190 SLSSLDSEEQESLRAAERLHNRTSRNSTGGISTHSLNEAELARDF-EKVVAKRNLVPVVPRIPLQ 253

  Fly  1186 K-----------RRDMEDHNYFVPHRNDLIENYLHNHEFVEVCMKILYQVELQRTTLEQMWGFSV 1239
            |           .:::::.| .|.|.:| .|:..|:...  :|::                  ..
Mosquito   254 KSISTPSIAPVRNQNVKEDN-LVSHLSD-SEDETHDRSL--LCVR------------------DK 296

  Fly  1240 EAELIENAE-RQDELCCYVSRVEDKSVAMHNGIIKGDEIM------VINGAIVSDLDMMYLESVL 1297
            ..|..|:.| |:.....:..:.:|||..      ||...:      |||.|...:..:.....:.
Mosquito   297 NEEYPEHHEKRRKRGSLFFRKKKDKSKT------KGQSSVCDACGAVINLATYKEHAVECKAKIA 355

  Fly  1298 QEEQSLSMMMRSSRTEPPDLVGIMRVTDDMIDSLVCPPPPTDPPVMSEEMITGLIVPAPGWNGTS 1362
            :|..:.::.:|.......|.|.::|  |:.:..     .|..|..:..|.|.|:.:       ..
Mosquito   356 KENVNSNLCIRYLHCSYSDDVPLIR--DEFLHE-----APIGPHDLGAEPILGVAI-------DE 406

  Fly  1363 KDLYSPEAESSPATSFVDPAAMAAQLAVGGVGGVVVGGLGVAKPTSRTSSFEIENLLKTAEQETR 1427
            .|.:||....                                         |:...||
Mosquito   407 HDSWSPSVPK-----------------------------------------EVVKALK------- 423

  Fly  1428 KSSPTGSVTSSVSTTALTPSRQLTDAEKLRKVVMELVDTERTYVKHLNNLLEHYLEPMKRETFLS 1492
                               .:|:    |.::.:.|.:.||:.:|:.|..:.:.::|.:::.  .|
Mosquito   424 -------------------DKQV----KRQEHIYEFIMTEKHHVQTLLVMQKVFVESLQKH--FS 463

  Fly  1493 NAEINALFGNIHEIVTFQRQFLQNLEESLDLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNHF--- 1554
            :..:..:|..:.|:......||:.|.        ..:.||     :|:.:|....|.:.:..
Mosquito   464 HLNLERMFPRLVELTELHTGFLRKLR--------LKQREH-----HVVDSIADILLDFFSSMSAQ 515

  Fly  1555 KL---YSSFCASHSKAQKVLH-PNEGNHALQEFL--AARNPKQQHSSTLESYLIKPIQRILKYPL 1613
            ||   |..||::|..|..... ...|::...|:.  ..:||..:.....|..|. ..||:.||||
Mosquito   516 KLKSAYGEFCSNHRSALDTFKCYMTGDNVFAEWYKHCQQNPLLKKKGIPECILF-VTQRLTKYPL 579

  Fly  1614 LLQQMRNLTDTRAD--EHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSC----- 1671
            |:..:  |..:|.|  |...|.:|:..::::...::......|:.....:...|...|||
Mosquito   580 LIDPL--LKSSREDKIEQEKLQKAMSLVKEILVDVDARVADKEKEDRQLEIFKRIDAKSCATFKK 642

  Fly  1672 ---KQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAM---CFVF---------------KS 1715
               |:...:|....|.:.||..|    ..|:.|....|..:   |..|               |:
Mosquito   643 DKFKKSDIISCNRKLRFEGVATL----MQGRSKMQTVLVVVLSDCLFFLLENSHKYSFFTPENKA 703

  Fly  1716 AVVFLCKERLRQKK----------------------KLMGVSSKNA-------------TNEVEI 1745
            .||.|.|..:|:|.                      |:.....||.             ::|.|.
Mosquito   704 GVVSLQKLLIREKAGTESRGIYIISSNPAYPEMYELKVQNPKDKNVWIQSIRAAVIDCPSDESEA 768

  Fly  1746 IRY------QVLIPVTEVQVRASSAKDMDSHF-----LWELIHLRSQL----QRRSEKV-----Y 1790
            ..|      |.:|.:.:..:|....|.....|     |.|.|.|:..|    ::.:|::     .
Mosquito   769 EDYMSMEQRQKIIDLKQANMREIICKMRQKDFEQAILLEEKIALQLSLLLDNEQNAEQLGPAVES 833

  Fly  1791 VLSN------------STADFRNAFLKTIRQI--IRESVRNMS--IPMKNFGGSSGSVSGHSSQG 1839
            .:||            .|.:.....|.:|::|  :..|:...:  :|:..   ||.||....|:.
Mosquito   834 FISNYGSYRDLISDDCDTIEIWKRVLNSIQEISSLASSLYTAATGLPLSR---SSSSVGERQSEL 895

  Fly  1840 MGSMGYPGNSQTL-----ERPKQQITI--VHGSHTLGKPKKKSGSQRHSAGNIDYDNLSGSQEAD 1897
            ..|...|..::|.     .|.||.||.  ..|.:.....|::..:..||:.: ..||.:....:.
Mosquito   896 FISPTLPKRAETFGGFDERRSKQLITAAGTGGGNLTMHSKREQQTNAHSSSS-PLDNYAALHVSH 959

  Fly  1898 DLPPSVGVVHYASGHTHGQQMQPAGFRGRSKTV---GDVTEITCSSPEPHQQ-----QQQQQQQQ 1954
            .|...:.::..........|.|...:|...||:   .|..|...:..:..|:     |:|::|::
Mosquito   960 HLHTLLCIISQQMTTIQSLQHQLNNYRENPKTLYRHNDQLEELRNLQDKLQEEKTAWQKQKEQEE 1024

  Fly  1955 QQLMQQGHAHAHPHPHPHPREPPPPPIRQPHLHHHSSDIERIDPGTKSEGEEDSQQGTIRPKATL 2019
            ::|.:...:.               ..:|..:.....||.:.......:.|..|.||.:...:..
Mosquito  1025 RELEETKKSQ---------------KAQQEQIRAEQEDIRQQREQLYRKMEILSSQGLLLSPSVA 1074

  Fly  2020 GRTPNHLTLSTTSTLSVGSTGSQARLIQSSHPPASY--QPVLM---KDLGK-------PSPDESE 2072
            ...|..:..|....|||............|.|.:|.  ..|.:   ||..:       |..:...
Mosquito  1075 LPIPTGVVTSLDEALSVSEEHHVDSAFAGSIPASSMIGSSVTIDRKKDKWRTASITKTPPANLVS 1139

  Fly  2073 LESESEVHAAQVAASFNLSLGSSGSLFASDARDNQTQS-----------RTQSQIHSLHP----- 2121
            ..:.::::|..:.....|.|.|..|..|.....|...|           .|.|.:..:.|
Mosquito  1140 ATNAAKINATNIKQQLPLKLSSLSSTKAGSTACNMVMSPNSGNVAAAAHGTNSGVTQMFPLKLAD 1204

  Fly  2122 --------SPRQSPRQSPRQSPKQDIEVIEIFESEAERLAASQQVAVVHQHPSDQARESGHKQRR 2178
                    |...:|..|...|....|:               ||:.|          :||:...|
Mosquito  1205 KKVCVAFISSTGTPNHSRTGSSPAVIQ---------------QQIQV----------QSGNPATR 1244

  Fly  2179 DRDRN--PTETDATARFMDKHLSDLEQENLTDGTCDIVAY---MESLREKAFDPQDLAEDTGDHQ 2238
            ....|  |..|...|             |:.:..|....|   .|..|.::.|...|:..|
Mosquito  1245 YVFYNMVPNFTFFCA-------------NVINVMCRTNTYPKIPERFRLRSSDNYPLSSGT---- 1292

  Fly  2239 QNDSSLSPEPQT-----------IVENTQQTVVVDIESPSRPATESDSTGNTTG-------PSER 2285
               ::.||.|||           .:.:|...:|     |:|.....:|||..|.       ||.:
Mosquito  1293 ---TANSPVPQTYQYSPSTHSMGAMSSTPPPLV-----PARNTAHHESTGQRTSSPISSRHPSLQ 1349

  Fly  2286 SGDETEEEATTSSLASHIVVIDKKVDKIFKAKSPE 2320
            |..::.:..|:.|            |...|||..|
Mosquito  1350 SPPKSSDTTTSKS------------DSNNKAKEEE 1372

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	8/29 (28%)
		PH	873..974	CDD:278594	4/15 (27%)
		TIAM1_RBD	1141..1219	CDD:176413	19/97 (20%)
		PDZ_signaling	1221..1308	CDD:238492	14/93 (15%)
		RhoGEF	1456..1647	CDD:238091	43/201 (21%)
		PH2_Tiam1_2	1650..1823	CDD:269957	48/269 (18%)
		PH	1693..1812	CDD:278594	35/205 (17%)
AgaP_AGAP007712	XP_308164.4	RhoGEF	433..614	CDD:279015	43/198 (22%)
AgaP_AGAP007712	XP_308164.4	PH_ARHGEF2_18_like	657..758	CDD:275432	19/104 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910