Protein Alignment sif and ARHGEF25

DIOPT Version :10

• Sequence 1: NP_001261446.1
• Gene: sif / 43892
• FlyBaseID: FBgn0085447
• Length: 2734
• Species: Drosophila melanogaster
• Sequence 2: NP_001104740.2
• Gene: ARHGEF25 / 115557
• HGNCID: 30275
• Length: 619
• Species: Homo sapiens
• Alignment Length: 776
• Identity: 156/776 - (20%)
• Similarity: 260/776 - (33%)
• Gaps: 247/776 - (31%)

  Fly  1236 GFSVEAELIENAERQDELCCYVSRVEDKSVAMHNGIIKGDEIMVINGAIVSDLDMMYLESVLQEE 1300
            |...|:|..|...|:|.                       |..|:.||:..:   .|  |:...|
Human    44 GTEPESERTEGDHRRDR-----------------------EHEVLAGALQPE---SY--SIAGSE 80

  Fly  1301 QSLSMMMRSSRTEPPDLVGIMRVTDDMIDSLVCPPPPTDPPVMSEEMITGLIVPAPGWNGTSKDL 1365
            .|:|....|....|..       ....:.|..|.|.|..|       ::||    ..|...||..
Human    81 GSISASAASGLAAPSG-------PSSGLSSGPCSPGPPGP-------VSGL----RRWLDHSKHC 127

  Fly  1366 YSPEAESSPATS------FVDPA-AMAAQLAVGGVGGVVVGGLGVAKPTSRTSSFEIENLLKT-- 1421
            .|.|.|:....:      .::|| |...:|....:...::.|     |..:|...|.|.|.:.
Human   128 LSVETEADSGQAGPYENWMLEPALATGEELPELTLLTTLLEG-----PGDKTQPPEEETLSQAPE 187

  Fly  1422 AEQETRKSSPTGSVTSSVSTTALTPSRQLTDAEKLRKVVMELVDTERTYVKHLNNLLEHYLEPMK 1486
            :|:|.:|.:                      .|:...|:.|||:||:.||..|..::|.|:..|.
Human   188 SEEEQKKKA----------------------LERSMYVLSELVETEKMYVDDLGQIVEGYMATMA 230

  Fly  1487 R----ETFLSNAEINALFGNIHEIVTFQRQ-FLQNLEESLDLEPDFNKFEHCGQFRNVLFAIGSA 1546
            .    |:......|  :||||.:|..:.|. |||.|:..|. :||:               :...
Human   231 AQGVPESLRGRDRI--VFGNIQQIYEWHRDYFLQELQRCLK-DPDW---------------LAQL 277

  Fly  1547 FLYYVNHFKLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPKQQ--HSSTLESYLIKPIQRIL 1609
            |:.:.....:|..:|.:..|::.|: ...|:...:|.      :||  |...|...||||:|||:
Human   278 FIKHERRLHMYVVYCQNKPKSEHVV-SEFGDSYFEEL------RQQLGHRLQLNDLLIKPVQRIM 335

  Fly  1610 KYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQP 1674
            ||.|||:......:....:...|.:|::.|..|.:..|:|..:....|  |:.......|...|
Human   336 KYQLLLKDFLKYYNRAGMDTADLEQAVEVMCFVPKRCNDMMTLGRLRG--FEGKLTAQGKLLGQ- 397

  Fly  1675 IDLSPGDLLYYGGVEWLNISDFLGKI-KKGLELHAMCFVFKSAVVFLCKERLRQKKKLMGVSSKN 1738
                        ...|:...:..|.: .:|.|...  |:|:..::|         .:.:|...:.
Human   398 ------------DTFWVTEPEAGGLLSSRGRERRV--FLFEQIIIF---------SEALGGGVRG 439

  Fly  1739 ATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNSTADFRNAF 1803
            .|....:  |:..|.|:.:.:..:...| ...|..........:||     |||..:......|:
Human   440 GTQPGYV--YKNSIKVSCLGLEGNLQGD-PCRFALTSRGPEGGIQR-----YVLQAADPAISQAW 496

  Fly  1804 LKTIRQII---RESVRNMSIPMKNFGGSSGSVSGHSSQGMGSMGYPGNSQTLERPKQQITIVHGS 1865
            :|.:.||:   |:.:..:..|::                            .:|.:.|      :
Human   497 IKHVAQILESQRDFLNALQSPIE----------------------------YQRRESQ------T 527

  Fly  1866 HTLGKPKKKSGSQRHSAGNIDYDNLSGSQEADDLPPSVGVVHYASGHTH--------GQQMQPAG 1922
            ::||:|:   |....|.|.|..    |.|              |.|.||        ...:.|.|
Human   528 NSLGRPR---GPGVGSPGRIQL----GDQ--------------AQGSTHTPINGSLPSLLLSPKG 571

  Fly  1923 FRGRS------KTVGDVTEITCSSPEPHQQQQQQQQQQQQLMQQGHAHAHPHPHPHPREPP 1977
            ...|:      :.:||:.:      .||..                    |...|.|:.||
Human   572 EVARALLPLDKQALGDIPQ------APHDS--------------------PPVSPTPKTPP 606

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		Tiam_CC_Ex	999..1096	CDD:408184
		RBD	1141..1207	CDD:128731
		PDZ	1220..1299	CDD:214570	12/62 (19%)
		RhoGEF	1456..1647	CDD:238091	54/197 (27%)
		PH2_Tiam1_2	1650..1823	CDD:269957	29/176 (16%)
		PHA03307	2227..>2510	CDD:223039
ARHGEF25	NP_001104740.2	RhoGEF	203..373	CDD:459876	54/194 (28%)
		PH2_Kalirin_Trio_p63RhoGEF	378..520	CDD:270061	29/203 (14%)