DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Arhgef28

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_006517581.1	Gene:	Arhgef28 / 110596	MGIID:	1346016	Length:	1714	Species:	Mus musculus

Alignment Length:	2013	Identity:	403/2013 - (20%)
Similarity:	676/2013 - (33%)	Gaps:	640/2013 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly   579 LLEYEMHLRNTLAKGMDAESYSLHTFEALLSQSM-----ENLEFAESLPGSNQRSPYPLRRPNAK 638
            |.:.|:.|..|:. |..:...|||. |:||..::     :...|...|||..:    .|:.||.:
Mouse   154 LTQLELPLGWTVL-GNSSLEVSLHR-ESLLHLAVRWALPKLFHFLLCLPGGVK----ALKLPNEE 212

  Fly   639 SSTLPLPPHRPLSTIRDKERDRDRDGYYSDRNELIRERERERDRGYLSDHNSSFSNSRCASCIGE 703
            ::|       ||        |....|.:|...|.|        ..:...|:..||..|    :.|
Mouse   213 ATT-------PL--------DLALQGGHSTLVEDI--------TNFQGSHSPGFSRLR----LNE 250

  Fly   704 SARAQWFRHSDGWRSGSSTIGSGSGHGMMTQQIPGSGHKRSPWDS---LPSLRQDSSLNDSGYKS 765
            .|..| |.||.  .:.:.|:...:.| ::...|  ...::..||.   :.:|.|::....:...|
Mouse   251 EATLQ-FVHSS--ETLTLTVNHTAEH-LLEADI--KLFRKYFWDRAFLVKALEQEAKTEKATMPS 309

  Fly   766 ARADSLEQRAEFIRQ-DSLRSEYLSDRESRYGIVQQASIES-TDSRMCYLTSSEISDDDRMSLTT 828
            ..|::.|:    :|. :|.||....:.:::       ||:| .|.      .||..|.||:.|..
Mouse   310 GAAETEEE----VRNLESGRSPSEEEEDAK-------SIKSQVDG------PSEHEDQDRLPLDR 357

  Fly   829 AVSDEDDGESVMASPYKAKATGTAASSF--------NCTGAVRKAGFLSVKKWLLRKKHQIELAR 885
            :.......:.|.||    .|.|..:...        ||. .:.:.|.|.:.
Mouse   358 SFDGLKKSKHVPAS----LAAGQLSDVLNGGDEVYANCM-VIDQVGDLDIN-------------- 403

  Fly   886 KRGWKGYWVCLKGTTLLFYPCDSREGRSVEAAPKHLIIVDGAIMQPIPEHPKRDYIFCLSTAFGD 950
                   ::.|:|.:....|   ..|||: ..|:       |.|..:|..
Mouse   404 -------YINLEGLSTHTSP---ESGRSM-LGPQ-------ACMHTLPPD--------------- 435

  Fly   951 AYLFQAPC-QVELENWVNSIHSACAAAFAR-HRGKTGTLHLLQEEIFRLEKAIESDHKLKHMAEL 1013
                .:|| :..:||...::.:|.:.:|.. ...:|..|: |...:...||  |..|..|..:.|
Mouse   436 ----TSPCGRPLIENSEGTLDAAASQSFVTPSSSRTSNLN-LSFGLHGFEK--EQSHLKKRSSSL 493

  Fly  1014 QQSVVTDQETRHQIQTQILQWEENLERLHCEQFRLRCYMASLQSGE---LPNPKSLLT------- 1068
             .::|.|.|.....:..|                  ||....||..   ||:...|.:
Mouse   494 -DALVADSEGEGGSEPPI------------------CYAVGSQSSPRTGLPSGDELDSFETNTEP 539

  Fly  1069 --HVSRPTKNTLNKLGVFTVSSFHAFICARSPSLLNNLLAGRGATKRRPPMLSRSNSGSSR--RS 1129
              ::||....:|:       |:.|:     ..|||:. :..|..:...|    :.:||.||  |.
Mouse   540 DCNISRTESLSLS-------STLHS-----KESLLSG-IRSRSYSCSSP----KISSGKSRLVRD 587

  Fly  1130 MQMNSRDEPEKTFKVAMPDNAYS------TVYLRDAMSVEE---------FLAS-ACARRNLNPM 1178
            ..:.|..|.::::....|.....      ..|:..|.|..|         ||.: ..:.||.:.|
Mouse   588 FTVCSTSEEQRSYSFQEPPGEKRIQEEEWDEYVIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKM 652

  Fly  1179 EHFVRVKKRRDMEDHNYFVPHRNDLIENYLHNHEFVEVCMKILYQVE-LQRTTL--EQMWGFSVE 1240
                   |.:|.::            :..::.|:||......:.|.. ..:|.|  |.:...:.:
Mouse   653 -------KNKDTKE------------KEKMNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCK 698

  Fly  1241 AELIENAERQDELCCYVSRVEDKSVAMHNGIIKGDEIMVINGAIVSDLDMMYLESVLQEEQSLSM 1305
            |...:..:.....|  ..:.::|    :|   |.....::..:.|.|:....|.  |....|:.:
Mouse   699 ANTHKGCKDAVPPC--TKKFQEK----YN---KNKPQSILGSSSVRDVPAPGLS--LHPSSSMPI 752

  Fly  1306 MMRSSRTEPPDLVGIMRVTDDMIDSLVCPPPPTDPPVMSEEM---ITGLIVPAPGWNGT--SKDL 1365
            .:.:.|.|              ..:.|.|...:.|....|..   :|.|......|...  |.:|
Mouse   753 GLPAGRKE--------------FAAQVHPLSRSVPGTTLESFRRAVTSLESEGDSWRSRSHSDEL 803

  Fly  1366 Y-----SPEAESSPATSFVDPAAMAAQLAVGGVGGVVVGGLGVAKPTSRTSSFEIENLLKTAEQE 1425
            :     ||..||......||                              ||..|:  |.:..||
Mouse   804 FQSMGSSPSTESFMMEDVVD------------------------------SSLWID--LSSDAQE 836

  Fly  1426 TRKSSPTGSVTSSVSTTALTPSRQLTDAEKLRKVVMELVDTERTYVKHLNNLL---EHYLEPMKR 1487
            ....|.:..|..|..      |||..|..|.:.|:.||:.||   |.|:..||   |.:.:.||.
Mouse   837 FEAESWSLVVDPSFC------SRQEKDVIKRQDVIFELMQTE---VHHIQTLLIMSEVFRKGMKE 892

  Fly  1488 ETFLSNAEINALFGNIHEIVTFQRQFLQNLEESLDLEPDFNKFEHC-GQFRN-VLFAIGSAFLYY 1550
            |..|.::.::.:|..:.|::...|.|..:::|        .:.|.| |..|| |:..||...
Mouse   893 ELQLDHSTVDKIFPCLDELLETHRHFFFSMKE--------RRQESCAGSDRNFVINQIGDIL--- 946

  Fly  1551 VNHF---------KLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPK-QQHSSTLESYLIKPI 1605
            |..|         ::|..||:.|.:|..:....:.|...|.|:..||.. ......:...::...
Mouse   947 VQQFSEENASKMKRIYGEFCSHHKEAMSLFKELQQNKKFQNFIKIRNSNLLARRRGIPECILLVT 1011

  Fly  1606 QRILKYPLLLQQMRNLTDTRADEHVHLCEAL---KGM--------------EKVAEHINEMQRIH 1653
            |||.|||:|::::...|..|.:||..||:||   |.|              :|..|.:|:::  :
Mouse  1012 QRITKYPVLVERILQYTKERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIE--N 1074

  Fly  1654 EEYGAIFD-HLFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAV 1717
            :.|..:.: |:||      ||.:......||:.|.|.|...:   |:.|..|.|     :....:
Mouse  1075 KTYTKLKNGHVFR------KQALLSQERALLHDGLVYWKTAT---GRFKDILAL-----LLTDVL 1125

  Fly  1718 VFLCKERLRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQ--------VRASSAKDMDSHFLWE 1774
            :||  :...||.....|..|.:     :|..|.|| ..||.        :.||||
Mouse  1126 LFL--QEKDQKYIFAAVDQKPS-----VISLQKLI-AREVANEERGMFLISASSA---------- 1172

  Fly  1775 LIHLRSQLQRRSEKVYVLSNSTADFRNAFLKTIRQIIRESVRNMSIPMKNFGGSSGSVSGHSSQG 1839
                       ..::|.:..::.:.||.:::.|:|.:...
Mouse  1173 -----------GPEMYEIHTNSKEERNNWMRRIQQAVESC------------------------- 1201

  Fly  1840 MGSMGYPGNSQTLERPKQQITIVHGSHTLGKPKKKSGSQRHSAGNIDYDNLSGSQEADDLPPSVG 1904
                                           |:::.|.               :.|:|:
Mouse  1202 -------------------------------PEEEGGR---------------TSESDE------ 1214

  Fly  1905 VVHYASGHTHGQQMQPAGFRGRSKTVGDVTEITCSSPEPHQQQQQQQQQQQQ----LMQQGHAHA 1965
                                .|.|....|.:|       .|.|:....|.||    |.::.|.:|
Mouse  1215 --------------------ERRKAEARVAKI-------QQCQEILSNQDQQICTYLEEKLHIYA 1252

  Fly  1966 -----------HPHPH----PHPREPP-PPPIRQPHLHHHSS---DIERIDPGTKSEGEEDSQQG 2011
                       |..||    |.|.||| ...:....|....|   .::....|..|:..|:|..|
Mouse  1253 ELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALREAESLQVAVKASKMGDVSQSSEESPGG 1317

  Fly  2012 TIRPKATLGRTPNHLTLSTTSTLSVGSTGSQARLIQSSHPPASYQPVL----MKDLGKPSPDES- 2071
            |:     |..||:...:..:.|.|:.:.|::.|......|  ..|.|:    :.|.|:.....|
Mouse  1318 TV-----LMDTPSTQDVPASPTASLVTEGTEGRGCWDVDP--GLQGVVTDLAVSDAGEKVEYRSF 1375

  Fly  2072 ELESESEVHAAQVAASFNLSLGSSGSLFASDARDNQTQSRTQSQIHSLHPSPRQS--PRQSPRQS 2134
            ...|:||:    :.|..||    :..|::..|.  .|...:..:||.|....|:|  |..|.|..
Mouse  1376 SGSSQSEI----IQAIQNL----TRLLYSLQAA--LTIQDSHIEIHKLVLQQRESLAPSHSFRGG 1430

  Fly  2135 PKQDIEVIEIFESEAERLAASQQVAVVHQHPSDQARESGHK----QRRDRDRNPT-------ETD 2188
            |.||.|.....|.:.|.||...::    ||...|.:...|:    |:|:::...:       |..
Mouse  1431 PLQDQEKSRYLEKQREELANIHKL----QHQFQQEQRRWHRTCDQQQREQEAQESWLQARERECQ 1491

  Fly  2189 ATARFMDKHLSDLEQENLTDGTCDIVAYMESLREKAFDPQDLAEDTGDHQQNDSSLSPEPQTIVE 2253
            :....:.:|.|:|:.:        :..|.:|| |:..:.|.:.|......:....|...    .:
Mouse  1492 SQEELLLRHRSELDHQ--------LQEYQQSL-ERLREGQRMVERERQKMRVQQGLLGH----CK 1543

  Fly  2254 NTQQTVVVDIESP-SRPATE----------------------SDSTGNTTGPSERSGDETEEEAT 2295
            :::|..:..:.|| |:..||                      |.:|.|...||....|....|.:
Mouse  1544 HSRQRSLPAVFSPGSKEVTELNRAESLCHENSFFINEAFGHMSLNTSNKPNPSGVPWDAHPLEGS 1608

  Fly  2296 TSSLASHIVVIDKKVDKIFKAKSPERPASTKVKGGRRQIYISPDRCKSQGSSPVRIIKIKSPRTS 2360
            ...|                |::.|.|...|:     .|...||     .:|.:........|.:
Mouse  1609 HFDL----------------ARTSESPTELKI-----DISQPPD-----VNSELWTTGPGHQRPA 1647

  Fly  2361 LSDQGKRLSVCSSRDNSQDRLSAGRRTPSPRRSSEGGGILKHTTGPDPGILKPPSSPAKSKSP 2423
            |.:..|     .|..|..|..|....:.||:.|::        .||.|..|   ::.||...|
Mouse  1648 LQENSK-----ESYKNVADLDSFQSESSSPQDSNQ--------RGPQPQTL---TTEAKLSLP 1694

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	18/126 (14%)
		PH	873..974	CDD:278594	15/101 (15%)
		TIAM1_RBD	1141..1219	CDD:176413	15/93 (16%)
		PDZ_signaling	1221..1308	CDD:238492	14/89 (16%)
		RhoGEF	1456..1647	CDD:238091	58/222 (26%)
		PH2_Tiam1_2	1650..1823	CDD:269957	36/181 (20%)
		PH	1693..1812	CDD:278594	25/126 (20%)
Arhgef28	XP_006517581.1	C1	666..711	CDD:237996	8/44 (18%)
		RhoGEF	861..1053	CDD:238091	56/205 (27%)
		PH_p190RhoGEF	1098..1198	CDD:275430	30/136 (22%)
		xseA	<1364..1520	CDD:234714	42/178 (24%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3519
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810