DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Mcf2

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_006527813.1	Gene:	Mcf2 / 109904	MGIID:	96932	Length:	1127	Species:	Mus musculus

Alignment Length:	1098	Identity:	220/1098 - (20%)
Similarity:	374/1098 - (34%)	Gaps:	313/1098 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly   893 WVCLKGTTLLFYPCDSREGRSVEAAPKHLIIVDGAIMQPIPEHPKRDYIFCLSTAFGDAYLFQAP 957
            |:.:|           |..|:...||.         :|...:|       |....|..|    .|
Mouse    43 WLAVK-----------RLPRAAAKAPN---------LQLNQDH-------CSFVPFTQA----VP 76

  Fly   958 CQVELENWVNSIHSACAAAFARHR----GKTGTLHL-LQEEIFRLEKAIESDHK-LKHMAELQQS 1016
            .||::             |.|.||    .|.|..:| |.:.:|..|..:....| :.|.  |.|.
Mouse    77 VQVDM-------------AMALHRVRIAEKYGKNYLCLLQAVFLAETGLLLQLKDINHF--LMQD 126

  Fly  1017 VVTDQETRHQIQTQILQWEENLERLHCEQFRLRC-----------YMASL--QSGE-----LPNP 1063
            :......|.:....|:.:.||     |   ..||           |:.|:  |||.     :...
Mouse   127 IAFLSGGRGKDNAWIITFPEN-----C---NFRCIPEEVIAKVLNYLTSIARQSGSDSRFTIILD 183

  Fly  1064 KSLLTHVSRPTKNTLNKLGVFTVSSFHAFICARSPSLLNNLLAGRG--------ATKRRPPMLSR 1120
            :.|.|..|  .|.:|.|:......|.|..:..|..|.|.......|        ..|....|||.
Mouse   184 RRLDTWAS--LKISLQKISASFPGSLHLVLVLRPTSFLQRTFTDIGFRFSQEDFMLKLPVVMLSS 246

  Fly  1121 SN---------------SGSSR--RSMQMNSRDEPEKTFKVAMPDNAY----------STVYLRD 1158
            .:               .|:.:  .|..:..|:..|| |.|.:.:.|.          .|...:|
Mouse   247 VSDLLTYIDDKQLTPELGGTLQYCHSEWIIFRNAIEK-FAVTVKEMAQMLQSFGTELAETELPQD 310

  Fly  1159 AMSVEEFLASACARRNLNPMEHFVRVKKRRDMEDHNYFVPHRNDLIENYLHNHEFV----EVCMK 1219
            .:|:||.||....|.:|...: ...|.|...:...|..||...:.:.:.|...:.:    :...|
Mouse   311 ILSIEEILAGRAERYHLLKND-LTAVTKEGKVLLMNLQVPATEETVSSSLECTQHINGDWQTIKK 374

  Fly  1220 ILYQVELQRTTLE--------------QMWGFS-------VEAELIENAERQ-DELCCYVSRV-- 1260
            :|.||....|..:              |:|.|.       .:.|.:.:.:|: .::...:::|
Mouse   375 LLAQVHDMETAFDGFWEKHQLKMEQYLQLWKFEQDFQEAVTQVEFLLSQQRELGDITGNLAQVKQ 439

  Fly  1261 --------EDKSVAMHNG----IIKGDEIMVINGAIVSDL------DMMYLESVLQEEQSLSMMM 1307
                    :|||..:...    |::|.: :..|.....||      ::.||..:|..|.....:.
Mouse   440 RLKKLEILDDKSQELLTKARIVILRGHK-LASNHHYALDLICQRCNELRYLSDILVNEIRTKRVQ 503

  Fly  1308 RSSRTEPPDLVGIMRVTDDMIDSLVCP------PPPTDPPVMSEEMITGLIVPAPGWNGTSKD-- 1364
            .|...:...|:...|...|..:.|:..      ....|.....:::...|.:..|..|...:.
Mouse   504 LSRTFKVHRLLQQARQCCDQGECLLASQGMDKFQTKEDAQKALQDVDNFLQMAMPFINYDIESLQ 568

  Fly  1365 -----LYSPEAESSPATSFVDPAAMAAQLAVGGVGGVVVGGLGVAKPTSRTSSFEIENLLKTAEQ 1424
                 |.|||.::.         ....||.:..:...........|..........:||:..:|.
Mouse   569 YEFDVLLSPELKAQ---------MQNIQLKLENIRSAFQNQQAGCKSLKEVPEGAFQNLVPASEN 624

  Fly  1425 ----------------------------ETRKSSPTGSVTSSVSTTALTPSRQLT--DAEKL--- 1456
                                        :.:||.......|:|...|:..|::..  .:.||
Mouse   625 VMRSRMIFFSPKHVGIGYSFFQACRIFLKVKKSWRQIHAQSNVKVEAVEDSQEKNSDQSPKLDNS 689

  Fly  1457 -----RKVVMELVDTERTYVKHLNNLLEHYLEPMKR-------ETFLSNAEINALFGNIHEIVTF 1509
                 ..|:.||:.|||.||:.|..:|..|...|..       ...|.|.: :.||||:.||..|
Mouse   690 LDILKNHVLNELIQTERAYVRELFTVLLGYRSEMDNPQMFDLMPPLLRNKK-DVLFGNMAEIYEF 753

  Fly  1510 QRQ-FLQNLEESLDLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHP 1573
            ... |:..||:..| .|:               .:|..||...:.|::|:.:|.:..:::.:..
Mouse   754 HNNIFMSRLEDCSD-APE---------------RVGPCFLERKDDFQMYAKYCQNKPRSELIWR- 801

  Fly  1574 NEGNHALQEFLAARNPKQQHSSTLESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKG 1638
               .::...|......|.:|...|:|||:||:|||.||.|||:::...: ...:....|.|||..
Mouse   802 ---KYSECAFFQECQRKLKHRLGLDSYLLKPVQRITKYQLLLKELLKYS-KEGEGTTKLKEALDS 862

  Fly  1639 MEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQPI----DLSPGDLLYYGGVE-WLNISDFLG 1698
            |.::.:.:|:                ..||.:....:    :|  |.::..|... ||.......
Mouse   863 MLELLKSVND----------------SMHQTAINGYVGNINEL--GKMVLQGSFNVWLGHRKGAT 909

  Fly  1699 KIK-----KGLELHAMCFVFKSAVVFLCKERLRQKK---KLMGVSSKNATNEVEIIRYQVLIPVT 1755
            |:|     |.::.|  .|:::.||:| ||.|....:   :....|.|:.....:       :.:|
Mouse   910 KMKDFARFKPMQRH--LFLYEKAVMF-CKRRFESGEGADRYPSYSFKHCLKMED-------VGIT 964

  Fly  1756 EVQVRASSAKDMDSHFLWELIHLRSQLQRRSEK--VYVLSNSTADFRNAFLKTIRQII 1811
            | .|:..:.|       :|:        |.|||  :|::.....|.:..:||.||:|:
Mouse   965 E-HVKGDNRK-------FEI--------RYSEKEEIYIVQAPNVDVKMLWLKEIRKIL 1006

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	19/95 (20%)
		PH	873..974	CDD:278594	14/80 (18%)
		TIAM1_RBD	1141..1219	CDD:176413	18/91 (20%)
		PDZ_signaling	1221..1308	CDD:238492	22/128 (17%)
		RhoGEF	1456..1647	CDD:238091	55/206 (27%)
		PH2_Tiam1_2	1650..1823	CDD:269957	36/177 (20%)
		PH	1693..1812	CDD:278594	29/129 (22%)
Mcf2	XP_006527813.1	SEC14	128..268	CDD:214706	29/149 (19%)
		SPEC	408..599	CDD:351773	30/200 (15%)
		RhoGEF	694..871	CDD:238091	54/198 (27%)
		PH-like	878..1009	CDD:388408	34/157 (22%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910