DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment sif and arhgap21a

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_001340279.1	Gene:	arhgap21a / 101883923	ZFINID:	ZDB-GENE-090918-5	Length:	1939	Species:	Danio rerio

Alignment Length:	1678	Identity:	305/1678 - (18%)
Similarity:	534/1678 - (31%)	Gaps:	532/1678 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly  1222 YQVE----LQRTTLEQMWGFSVEAELIENAER---QDEL--------------CCYVSR------ 1259
            |||.    |:..:.|::.|    ||...|..|   ||.|              |.|:.|
Zfish   447 YQVSSVLPLRSASQERLGG----AECARNWPRSASQDALPSTPTGVPKPRAYSCDYLGRNSDIAT 507

  Fly  1260 -VEDKSVAMHNG------IIKGDEIMVINGAIVSDLDMMYLESVLQEEQSLSMMMRSSRTEPPDL 1317
             :.||.......      :.||||...                   ..||.|:.|...||:  ||
Zfish   508 SIVDKKACCRTDTEEWLLVHKGDEARA-------------------SRQSSSLRMVPHRTQ--DL 551

  Fly  1318 VGIMRVTDDMIDSLVCPPPPTDPPVMSEEMITGLIVPAPGWNGTSKDLYSPEAES---------- 1372
            ..                     |........||.: .|.:|..:..|.:..|||
Zfish   552 FS---------------------PEKRGGSYPGLPI-TPLFNRGTDSLLNSRAESLGNNCTPSVN 594

  Fly  1373 ----SPATSFV-DPAAMAAQL---AVGGVGGVVVGGLGVAK-------------------PTSRT 1410
                .||.::| ||:..||..   |:..:.|.:....|:.|                   |..|.
Zfish   595 RSSLPPAKNYVSDPSTAAASYVASALASIQGHLGSNTGLVKDQRTPLIANHMPHNAKQLQPRGRA 659

  Fly  1411 SSFE--IENLLK-------TAEQETRKSSPTG--SVTSSVSTTALTPSRQLTDAEKLRKVV---- 1460
            .|.:  :..:::       :|..:..:|.|.|  |:.:|..|....||...|.|.|  |.|
Zfish   660 DSLQEPVREVIRPGRSSSCSAASKLHRSKPGGCKSILTSNGTVHQQPSCTETQANK--KAVDGIE 722

  Fly  1461 -----MELVDTER---------TYVKHLNNLLEHYLEPMKRET-FLSNAEINAL----FGNIHEI 1506
                 :.:|..|:         :|:..:|:......:|....| :|.|.....:    .|:..:|
Zfish   723 GPDATVVVVRREKSGSQPIRPHSYILAVNDTQGVAEQPPNSNTCWLPNDTRREMHMRKVGDQCKI 787

  Fly  1507 VTFQRQFLQNLEESLDLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVL 1571
                 ....||::|||..|..::........:......||   .::...:.::...|.:....|:
Zfish   788 -----SISSNLDDSLDSIPFIDEPSSSSNDHDTTHIPASA---VISSTPVITTIPPSPTSPTPVI 844

  Fly  1572 HP--NEGNHALQEFLAARNPKQQHSSTLESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCE 1634
            ..  :....:|:..:...:.|.:.|.:.:..              |...|.....|:.:|.|
Zfish   845 RRQLSHDQDSLRLTILESDSKTERSRSFDEG--------------LDNYREERRGRSIKHTH--- 892

  Fly  1635 ALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGK 1699
            ..||:.|..:      |..|:.|:       :...:.....|.:....||:..:...........
Zfish   893 GFKGLRKAVD------RCSEDSGS-------RRDSNSDVFTDATKEGSLYFRQLNSEKSKRVGSS 944

  Fly  1700 IKKGLELHAMCFVFKSAVVFLCKERLRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSA 1764
            ::...:::|   |.:...::|.|:      |..|.::.|:..|.|.:...:...:.::....:..
Zfish   945 MRSWKQIYA---VLRGHCLYLYKD------KRDGQTNANSQIEEEPLPISIKACLIDISYSDTKR 1000

  Fly  1765 KDM------DSHFLWELIHLRSQLQRRSEKV----YVLSNSTADFRNAFLKTIRQIIRESVRNMS 1819
            |::      |..:|:       |.:.|.|.:    .:..||..|..||.: |...:|...::..:
Zfish  1001 KNVLRLTTSDCEYLF-------QAEDREEMLSWIRVIQENSNLDEENATV-TSTDLINRKIKEYN 1057

  Fly  1820 IPMKNFGGSSGSVSGHSSQGMGSMGYPGNSQTLERPK---QQITIVHGSHTL--GKPKKKSGSQR 1879
            ..|.                      |.:|:|...||   |.::|   ..||  ||.:.|:.|.
Zfish  1058 TLMS----------------------PPSSKTEPSPKASRQSLSI---RQTLLGGKGENKATSP- 1096

  Fly  1880 HSAGNIDYDNLSGSQEADDLP---------------------PSVGVVHYASGHTHGQQMQPAGF 1923
            ||. |.::|..:..::....|                     ||.||       |.|.::.....
Zfish  1097 HSP-NKEHDRKAMHKDETSPPKDKGTWRKGIPGLMRKPFEKKPSPGV-------TFGVRLDDCPP 1153

  Fly  1924 RGRSKTVGDVTEITCSSPEPHQQQQQQ-----------QQQQQQLMQQGHAHAHPHPHPHP---- 1973
            ...::.|..:.|:.|:..|....:...           ...|::|..:|.|..........
Zfish  1154 AQNNRFVPLIVEVCCNLVEERGLEYTGIYRVPGNNAAISIMQEELNNKGMADIDIQDDKWKDLNV 1218

  Fly  1974 ---------REPPPP------------------PIRQPH-----LH----HHSSDIERIDPGTKS 2002
                     |:.|.|                  |:.:..     ||    ||...::.:....|:
Zfish  1219 ISSLLKSFFRKLPEPLFTNERYADFIDGNRIEDPVERLKVLKRLLHELPDHHYETLKFLSAHLKT 1283

  Fly  2003 EGEEDSQQGTIRPK-------ATLGRTP----NHLTLSTTSTLSVGSTGSQARLIQ--------- 2047
            .. |:|::..:.|:       .||.||.    .|:.........:..|     |||
Zfish  1284 VA-ENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVET-----LIQHYDWFFTEE 1342

  Fly  2048 -SSHPPASY--------QPV-----LMKDLGKPSPDESELESESEVHAAQVAASFNLSLGSSGSL 2098
             .:.|..:.        |||     |:.::|:......|:.......:|:       |.||.||
Zfish  1343 GDNEPTTTVQEESAVESQPVPNIDHLLTNIGRTGTSPGEVSDSPTSDSAK-------SKGSWGS- 1399

  Fly  2099 FASDARDNQTQSRTQSQIHSLHPSP-RQSPRQSPRQSPKQDIEVIEIFESEAERLAASQQVAVVH 2162
                .:|:.::...|.::.|:..:. |:..:...:..|....:.::...::.......|
Zfish  1400 ----GKDHCSREFLQPRVSSIFAAANRKRKKHKEKLQPSSSDDDLDTMFTKNNNPTQKQ------ 1454

  Fly  2163 QHPSDQARESGHKQRRDR----DRNPTETDATARFMDKHLSDLEQENLTDGTCDIVAYMESL--- 2220
            .|.|.:..|:|......:    |::....::|.:...:|.:..   .:.|.|..:::....|
Zfish  1455 SHQSQEEDEAGRSSSCGKSLVGDKDGISAESTPKSKKEHRNSF---FMKDKTSSVLSPSPKLSCS 1516

  Fly  2221 ----REKAFDPQDLAEDTGDHQQNDSSLSPEPQTIVENTQQTVVVDIESPSRPATESDSTGNTTG 2281
                |.|:..|:             .|:| :|.:.::.|...:.......|.|.|.....|.||.
Zfish  1517 PGFNRRKSGPPK-------------CSIS-DPSSQLDETTSDLGTMSSGASVPRTRPRKWGTTTP 1567

  Fly  2282 PS-------------ERSGDETEEEATTSSLASHIVVIDKKVDKIFKAKSPERPASTKVKGG--- 2330
            ||             ......|.:.:||||     ......::.|  ..|||..:.|:.:||
Zfish  1568 PSIDLLAAPGGASVTAEVSSITSDYSTTSS-----TTFLTGMESI--TLSPEVQSVTESRGGDEA 1625

  Fly  2331 --RRQIYIS---PDRCKSQGSSPV---RIIKIKSPRTSLSDQ-----------GKRLSVCSSRDN 2376
              .|...||   |....|:...|:   .|.:::.|.|..|..           ..:|..|   |:
Zfish  1626 DDERSELISEGRPMETDSESDFPMFSPAIGRLEMPNTEDSSNTTPKLACRLLPSHKLIEC---DS 1687

  Fly  2377 SQDRLSAGRRTPSPRRSSEGGGILKHTTGPDPGILKPP--------------------SSPAKSK 2421
            ...|.|..::|.|......|.|      |.:.|:.|..                    |:|::|:
Zfish  1688 LSRRRSLRQKTDSDSSVEAGAG------GGNQGVSKNESNRLCRVLEVMKRGRSTSSLSAPSRSE 1746

  Fly  2422 SPDRSCLKKGGPSHVCSVETLSPRVSPRGSMDHL----------SPDRGMTSSSCLRHSPRSSFD 2476
            |.....     |.|:...|.|..|:  |||.|.:          :..||.......||:
Zfish  1747 SDQVET-----PWHLKITERLKFRL--RGSADDMFGVGTQKTRSTETRGKKKGIRRRHT------ 1798

  Fly  2477 SHGESDH-NLDVPHGSRKRSMSAHGSFETGTRPRAQETYQYYPVYDNQTCSDHYVAAAPTGRYGG 2540
            ..|:.|. .|||.|..|::     |..:.|....|.|..:  |..|:|..|......:...|.|
Zfish  1799 MGGQRDFAELDVIHDWREQ-----GGVDQGAELPAVERLR--PKGDSQNMSIRDWIVSEHRRRG- 1855

  Fly  2541 GTSSRSRLSKSLERSTSRDSTTTSSSYGHRAYGVSPDRNYVVFSSGPLRSQSA 2593
              |..|:.|..||....:::..|..::        |..:..:.||..|.|||:
Zfish  1856 --SQGSQASTELEGQAGKETIETDKTH--------PRNSEQLSSSASLCSQSS 1898

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413
		PDZ_signaling	1221..1308	CDD:238492	25/119 (21%)
		RhoGEF	1456..1647	CDD:238091	33/215 (15%)
		PH2_Tiam1_2	1650..1823	CDD:269957	28/182 (15%)
		PH	1693..1812	CDD:278594	21/128 (16%)
arhgap21a	NP_001340279.1	PDZ_signaling	53..128	CDD:238492
		PH_ARHGAP21-like	923..1037	CDD:269955	19/129 (15%)
		RhoGAP_ARHGAP21	1143..1339	CDD:239860	31/201 (15%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910