DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Plekhg2

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_006539499.1	Gene:	Plekhg2 / 101497	MGIID:	2141874	Length:	1366	Species:	Mus musculus

Alignment Length:	1581	Identity:	329/1581 - (20%)
Similarity:	528/1581 - (33%)	Gaps:	480/1581 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly  1400 GLGVAKPTSR---------TSSFEIENLLKTAEQETRKSSPTGSVTS-SVSTT------------ 1442
            ||.:.||:.|         .....:.:....|:..|..:||.||.:| |:||.
Mouse     7 GLSLPKPSLRLGCGHQGEVCDCAAVSDTPTAAQAATTMASPRGSGSSTSLSTVGSEGDPSPACSA 71

  Fly  1443 ----------------------ALTPSRQLTDAEKLRKVVMELVDTERTYVKHLNNLLEHYLEPM 1485
                                  ::.|||       |.:|..|:|:|||.||:.|.:::|.||.|:
Mouse    72 SRPEPLPEPPIRLHLLPVGIQGSVKPSR-------LERVAREIVETERAYVRDLRSIVEDYLGPL 129

  Fly  1486 K--RETFLSNAEINALFGNIHEIVTFQRQFLQNLEESLDLEPDFNKFEHCGQFRNVLFAIGSAFL 1548
            .  |...|:..::..||.||.:|..|..:.|::|             |.|..    ...|...|:
Mouse   130 MDGRALGLNMEQVGTLFANIEDIYEFSSELLEDL-------------EGCSS----AGGIAECFV 177

  Fly  1549 YYVNHFKLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPKQQHSSTLESYLIKPIQRILKYPL 1613
            .....|.:|:.:|.::..:..:|.....:.....:|..|..:.:||..|:|:|:||:||||||.|
Mouse   178 QRSEDFDIYTLYCMNYPSSLALLRELSVSPPATLWLQERQAQLRHSLPLQSFLLKPVQRILKYHL 242

  Fly  1614 LLQQM----RNLTDTRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQP 1674
            |||::    ....|:...|.|.  ||:..|..||.:||:|:| .:|:.|....:  |.:......
Mouse   243 LLQELGKHWAEGPDSGGREMVE--EAIVSMTAVAWYINDMKR-KQEHAARLQEV--QRRLGGWTG 302

  Fly  1675 IDLSP-GDLLYYGGVEWLNISDFLGKIKKGLELHA---MCFVFKSAV---------------VFL 1720
            .:||. |:|:..|        .|.|....|..|..   :.|:|...:               :|.
Mouse   303 PELSAFGELVLEG--------TFRGGGGGGPRLRGGERLLFLFSRMLLVAKRRGPEYTYKGHIFC 359

  Fly  1721 CKERLRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQV----RASSAKDMDSHFLWELIHLRSQ 1781
            |.         :.||        |..|..:...|:::.:    ....||:.:...||  ||...:
Mouse   360 CN---------LSVS--------ETPRDPLGFKVSDLTIPKHRHLFQAKNQEEKRLW--IHCLQR 405

  Fly  1782 LQRRSEKVYVLSNSTADFRNAFLKTIRQIIRESVRNMSIPMKNFGGSSGSVSGHSSQGMGSMGYP 1846
            |...:....:.:.:            :|::.|:..:.:...|:....                 |
Mouse   406 LFFENHPASIPAKA------------KQVLLENSLHCAPKSKHIPEP-----------------P 441

  Fly  1847 GNSQTLERPKQQITIVHGSHTLGKPKKKSGSQRHSAGNIDYDNLSGSQEAD--------DLPPSV 1903
            .:.....||:.......|........:.|||:|....:.:|..|.|...:.        .|||..
Mouse   442 TSPLDSPRPRDAPGFTPGRRNPAPSPRLSGSRRGRRQSGEYPPLEGDPGSQWPGLLTSFFLPPEP 506

  Fly  1904 G--------------VVHYAS-GHTH-GQQMQPAGFRGRSKTVGDV---------TEITCSSPEP 1943
            .              |.|..| |..| ..::||....|..:.:.|.         |.||
Mouse   507 AKEAYVIFPQNDKPQVKHAGSEGELHPSSELQPVSASGPPEDLEDAGPPTLDPSGTSIT------ 565

  Fly  1944 HQQQQQQQQQQQQLMQQGHAHAHPHPHPHPREP----------PPP----PIRQPHLHHHSSDIE 1994
                   ::..:.|.|:|...:.|..|..|:.|          |.|    |.|:........|:|
Mouse   566 -------EEILELLNQRGLRDSGPATHDIPKFPRDSRVPVESEPLPFQSLPSRESSEEEEEEDLE 623

  Fly  1995 RID----PGTKSEGEEDSQQGTIRPKATLGRTPNHL-------------------------TLST 2030
            ..:    |....||.|.|....|....:|...|:.:                         .||.
Mouse   624 TDEREPSPLHVLEGLEGSSAAEIPCIPSLTDIPSEVPSLPEIPEAPCLPCLSDISGVFEVPCLSP 688

  Fly  2031 TSTL----SVGSTGSQARLIQSSHPPASYQPVLMKDLGKPSPDESEL----------ESESEVHA 2081
            |||:    |:.:|        .|.|..|:.|..:::..:|.....||          ||.||...
Mouse   689 TSTVPDIPSLATT--------PSFPCGSWLPGPLQEAAQPQATRRELLSGSNPGRLSESPSESRE 745

  Fly  2082 AQ----VAASFNLSLGSSGSLFASDARDNQTQSRTQSQIHSLHPSPRQSPRQSPRQSPKQDIEVI 2142
            .|    ...||:.....:|:  :......:.:.|:.|:|.|...:..|.  |..|....:.:.::
Mouse   746 GQEDDTEGVSFSAVQREAGT--SVQGFPEELEYRSCSEIRSAWQALEQG--QLARPGFPEPLLIL 806

  Fly  2143 EIFESEAERLAASQQVAVVH-QHPSDQARE-----SGHKQRRDRDRNPTETDATARFMDKHLSDL 2201
            |  :|:....:.|.:..:.| :..:.:.||     |...|:..|......|:|..| ..:.|:..
Mouse   807 E--DSDLRGGSTSGKTGMPHSERSASRVRELARLYSERIQQMQRAETRASTNAPRR-RPRVLAQP 868

  Fly  2202 E------QENLTDG---------TCDIVAYMESLRE----------KAFDPQDL-AEDTGDHQQN 2240
            :      ||....|         .|::...:...:|          :|..|..: .:|......|
Mouse   869 QPSPCPPQEEAEPGALPAFGHVLVCELAFPLNCTQESVPLGPAVLVQAATPLCIQGDDLSGQNLN 933

  Fly  2241 DSSLS----------PEP-----QTIVENTQ--QTVVV------DIESPSR---PAT---ESDST 2276
            .|.||          |.|     |:..:|.|  .|.::      |::.|:.   |.|   :|...
Mouse   934 VSDLSKQGHLSSNSIPPPVPLPGQSNFQNIQVPSTSLLPKQEPPDVQVPTASTLPDTSQLQSQVP 998

  Fly  2277 GNTTGPSERSGDETEEEATTS----SLASHIVVIDKK---VDKIFKAKSPERPASTKVKGGRRQI 2334
            ..|.....|:..|.:.::|||    ...:..|.:.|:   .|.....|:|          |..|.
Mouse   999 AATPSAGHRNCVEIQVQSTTSLPGQECQADTVALSKQEGHEDSQNPNKAP----------GAEQR 1053

  Fly  2335 YISPDR-CKSQGSSPVRIIKIKSPRTSLSDQGKRLSVCSSRDNSQDRLSAGRRTPSPRRSSEGGG 2398
            .:|.|: ....|..||      ||          |.||:|..:.|    ....||.|
Mouse  1054 DVSIDQGLAVVGGRPV------SP----------LPVCTSSPDQQ----IPATTPLP-------- 1090

  Fly  2399 ILKHTTGPD---PGILKPPSSPAKSKSPDRSCLKKGGPSHVCSVETLSPRVSPRGSMDHLSPDRG 2460
              ..|..||   ||.|..|:...:   ||  |.....|....|.:...|.|.|...:..|:..|.
Mouse  1091 --LSTDFPDMEGPGALPLPTQEGR---PD--CSIPCNPLPSLSQDVQVPAVIPVSQLQGLTDTRA 1148

  Fly  2461 MTSSSCLRHSPRSSFDSHGESDHNLDVPHGSRKRSMSAHGSFETGTRPRAQETYQYYPVYDNQTC 2525
            ....|  .|....:.:..|......|.|   ..|.:|:.|...|...|.:...   .|:.: |.|
Mouse  1149 TVPLS--SHKQEDAPECLGPEPSLTDTP---APRLLSSLGQQNTTDGPVSAAA---VPLTE-QGC 1204

  Fly  2526 SD----------HYVAAAPTGRYGGGTSSRSRLSKSLERSTSRD-STTTSSSYGHRAYGVSP--D 2577
            |.          ......|..|   |..||...|:||:.:.... |.:|....|..|..:|.  .
Mouse  1205 SQDLQGLITSPVQTTMELPKPR---GLVSRVATSESLDLTPPHSPSLSTRQLLGPSAAALSRYLA 1266

  Fly  2578 RNYVVFSSGPLRSQSAENTFSSQPIYDP------LPRHPPQPLHQPPMHHQQQQQQQQQQQYMSY 2636
            .:|:..|....:....|.|.:||..:..      .|..||||  |||....::         :||
Mouse  1267 ASYISQSLARRQGPGGEGTVASQGHWSSSAPTSRAPSPPPQP--QPPAPPARR---------LSY 1320

  Fly  2637 APEMGGYTGCQHDPQGGYQLSSSAPEYTTCIDCLYQKRPSXHKSRVRQSRTRKKTPRGMVVVSAQ 2701
            |..:....|      ||.:|                 ||:  |::||.:..       .::.:..
Mouse  1321 ATTVSIQVG------GGGRL-----------------RPA--KAQVRLNHP-------ALLAAPH 1353

  Fly  2702 PTATGGIGFVPEMGSSEAPGG 2722
            |.|         :|.|:.|||
Mouse  1354 PGA---------VGPSQGPGG 1365

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413
		PDZ_signaling	1221..1308	CDD:238492
		RhoGEF	1456..1647	CDD:238091	59/196 (30%)
		PH2_Tiam1_2	1650..1823	CDD:269957	31/195 (16%)
		PH	1693..1812	CDD:278594	21/140 (15%)
Plekhg2	XP_006539499.1	RhoGEF	103..278	CDD:366202	58/193 (30%)
		PH_PLEKHG1_G2_G3	260..410	CDD:270063	39/181 (22%)
		PTZ00449	<351..742	CDD:185628	80/459 (17%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1291
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.940