DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and tiam1b

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_009290217.1	Gene:	tiam1b / 100330358	ZFINID:	ZDB-GENE-120221-1	Length:	1562	Species:	Danio rerio

Alignment Length:	1722	Identity:	526/1722 - (30%)
Similarity:	789/1722 - (45%)	Gaps:	345/1722 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly   537 QQSSGQHPQQQQGPPQRSSRHQHH-----HQQAPNVTPTP-GSPTSRLLLEYEMHLRNTLAKGMD 595
            :..||.|.....|..|.|.:|...     .:|......|| |.|..          ||:......
Zfish     5 ESHSGGHGYYGDGQRQLSRKHMSRSLRLSSKQTYCTRRTPSGKPEH----------RNSETSTRS 59

  Fly   596 AESYSLHTFEALLSQSMENLEFAESLPGSNQRSPYPLRRPNAKSSTLPL-----PPHRPLSTIRD 655
            :.:.|:.  ::|....:|....::.||..:.    |:.......:..|:     |||.|  .::.
Zfish    60 SSTPSIP--QSLADHGLEPFNDSDLLPDFSS----PIWVDRVAMNLRPMSFHNQPPHSP--AVKP 116

  Fly   656 KE-------------RDRDRDGYYSDRN---ELIRERERERDRGYLSDHNSSFSNSRCASCIGES 704
            .|             ||..:||:...|:   ::.||...|.....||..:.:.:.....:....|
Zfish   117 PEENAQVQHTRSPTTRDASQDGFKKKRSKSADMWREDSLEVSLSDLSQEHMTSTEEMADTPDTHS 181

  Fly   705 AR-------------------AQWFR--------------HSDGWRSGSSTIGSGSGH----GMM 732
            |:                   |:|.:              ..||...|:.|:.....|    |..
Zfish   182 AQDRANSLDELYGTKPSHGRYARWHKGGAGDEEKMMMSPCEDDGAAYGAFTLPCRRSHCLSEGPT 246

  Fly   733 TQQIPGSGHKRSPWDSLPSLRQDSSLNDSGYKSAR-ADSLEQRAEFIRQDSLRSEYLSDRESRYG 796
            .||....|.:......: :....|...|||..... |||     |..:..|.|.:.:|...|.|.
Zfish   247 NQQGVMQGRRAQTIQDV-TAADGSDYEDSGIDGVTVADS-----ELSQHQSRRYKTMSVSFSAYS 305

  Fly   797 IVQQASIESTDSRMCYLTSSEISDDDR-----------MSLTTAVSDED------DGES--VMAS 842
            .....:...:||......::..:|:.:           .|:..|.:.||      |.:|  .::|
Zfish   306 TPAGGAFTGSDSGSSSGAAATNTDNTQGVYENFRQELEQSVCPAEAPEDRSCAFSDEQSSVTLSS 370

  Fly   843 PYKAKATGTAASSFNCTGAVRKAGFLSVKKWLLRKKH-QIELARKRGWKGYWVCLKGTTLLFYPC 906
            .|... |....|.....|.|||||.|:||.:|:.||: ::|||.:|.||.|||.|||.||.||..
Zfish   371 AYHTH-TDPLLSVSAVQGTVRKAGRLAVKNFLVHKKNKKVELAPRRKWKSYWVSLKGCTLFFYET 434

  Fly   907 DSREGRSVEAAPKHLIIVDGAIMQPIPEHPKRDYIFCLSTAFGDAYLFQAPCQVELENWVNSIHS 971
            |.|.|....:.|||.:..:.:|:|.:|||||:|::||||.:.||::|||...|.|||||:..|||
Zfish   435 DGRSGIDHNSVPKHAVWAENSIVQAVPEHPKKDFVFCLSNSLGDSFLFQTCSQTELENWITGIHS 499

  Fly   972 ACAAAFARHRGKTGTLHLLQEEIFRLEKAIESDHKLKHMAELQQSVVTDQETRHQIQTQILQWEE 1036
            |||||.||...:..|:.||:.||.:||:.|:.|.|:|.|.::|.|.|||.:.|..|..||..||:
Zfish   500 ACAAAVARQHHREDTVRLLRAEIRKLEQKIDMDEKMKKMGDMQLSAVTDAKKRKTILEQIFLWEQ 564

  Fly  1037 NLERLHCEQFRLRCYMASLQSGELPNPKSLLTHVSRPTKNTLNKLGVFTVSSFHAFICARSPSLL 1101
            |||:.|.:.||.|||::|||.|||||||.||...|||||..:.:||:|:||||||.:.||:.|
Zfish   565 NLEQFHMDLFRFRCYLSSLQGGELPNPKRLLAFASRPTKLAMGRLGIFSVSSFHALVAARTES-- 627

  Fly  1102 NNLLAGRGATKRRPPMLSRSNS------------------------------------------- 1123
                    ..:||...:|||.|
Zfish   628 --------GVRRRTQAMSRSTSKRKSRFSSLWGLDTHSKRKSKGHPSIDQVFAEGDDPVKSPLEG 684

  Fly  1124 ---GSSRRSMQ-----------------MNSRDEPEKTFKVAMPDNAYSTVYLRDAMSVEEFLAS 1168
               |||..:|:                 :.|||....:: |.:|::......::...|..:.|.:
Zfish   685 MFEGSSSETMKDTKTPVKGLPRPNTDHDIWSRDHLTPSW-VLLPNDQPVLAIIQPGESALDMLET 748

  Fly  1169 ACARRNLNPMEHFVRVKKRRDMEDH-NYFVPHRNDLIENYLHNHEFVEVCMKILYQVELQRTTLE 1232
            .|....|:..:|:||:|..  :|:. .:::|...:.|.:.|:..  :|:|.||...::..|....
Zfish   749 ICKAHQLDATKHYVRLKFL--IENQVQFYIPKPEEDIYDLLYKE--IELCCKIRKVIQFDRDESC 809

  Fly  1233 QM-WGFSVEAELIENAERQDELCCYVSRVEDKSVAMHNGIIKGDEIMVING---AIVSDLDMMYL 1293
            .: :|||:.. :.|:..:|    .|::.|....:|...|:..||||:.:||   ..::..||
Zfish   810 MIGYGFSISV-VEEDGVQQ----LYITDVRAGGLAFAKGLNAGDEILQLNGKDSRTLTFADM--- 866

  Fly  1294 ESVLQEEQSLSMMMRSSRTEPP----DLVGIMRVTDDMIDSLVCPPPPTDPPVMSEEMI----TG 1350
             .|...:.|||:.:   .|.||    .|..:.....|.:|:|.     ||....|:|.|    .|
Zfish   867 -KVAFAQVSLSLTV---NTLPPVERRQLCYLPPRRSDAVDNLY-----TDIFSQSQEEILDDGVG 922

  Fly  1351 LIVPAPGWNGTSKDLYSPEAESSPATSFVDPAAMAAQLAVGGVGGVVVGGLGVAKPTSRTSSFEI 1415
            |||...|                  .|..|.:.:.|:....|            |.|.:.::|
Zfish   923 LIVETSG------------------DSLDDDSELFAEFDDYG------------KSTEQVAAF-- 955

  Fly  1416 ENLLKTAEQETRKSSPTGSVTSSVSTTALTPS----RQLTDAEKLRKVVMELVDTERTYVKHLNN 1476
               .::..:....||.:.|.:|...:::|.||    |||:||:|||||:.|||:|||||||.||.
Zfish   956 ---CRSLHEMNPSSSESVSSSSVPESSSLPPSTATPRQLSDADKLRKVICELVETERTYVKDLNC 1017

  Fly  1477 LLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFLQNLEESLDLEPDFNKFEHCGQFRNVLF 1541
            |:..||.|:::|:||:..|::.||||:.|::.||.:||:.||:...|.||..|.|...:|:.:||
Zfish  1018 LIGRYLTPLQKESFLTQDELDVLFGNLPEMLEFQVEFLKTLEDGTRLVPDLEKLERVEEFKKILF 1082

  Fly  1542 AIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPKQQHSSTLESYLIKPIQ 1606
            ::|.:||||.:.||:||:|||||:|..|||...:.:.|.:.||..||||||||||||||||||||
Zfish  1083 SLGGSFLYYADRFKIYSAFCASHTKVPKVLVKAKTDAAFKAFLDERNPKQQHSSTLESYLIKPIQ 1147

  Fly  1607 RILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSC 1671
            |:|||||||:::.:|||..::||.||..|:|.|..||.||||||:||||:||:||.|..:.....
Zfish  1148 RVLKYPLLLRELHSLTDPDSEEHYHLNVAMKAMNTVASHINEMQKIHEEFGAVFDLLISEQTGEK 1212

  Fly  1672 KQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKERLRQKKKLMGVSS 1736
            |:..|||.||||.:..|.|:|.|..|||.||  |.....||||:||||:||:..:|||| ||.|.
Zfish  1213 KEVADLSMGDLLLHSSVTWINPSSSLGKCKK--EPQLATFVFKTAVVFVCKDYSKQKKK-MGGSH 1274

  Fly  1737 KNATN--EVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNSTADF 1799
            :.:.:  |.:..|::.:||...:|||..|..|.:|..:.|::|::|:.:.|.|:|:.|..|:.:.
Zfish  1275 RASVSGEEKDPFRFRHMIPTDALQVRTLSNTDGESAAVCEIVHIKSESEGRPERVFQLCCSSPES 1339

  Fly  1800 RNAFLKTIRQIIRESVRNM-----SIPMKN----FGGSS-GSVSGHSSQGMGSMGYPGNSQTLER 1854
            :..||||:..|:|:..|..     |:|:..    |||.. .::.|                  .|
Zfish  1340 KKDFLKTVHSILRDKQRRQLLKTESLPLSQQYVPFGGKRLCALKG------------------AR 1386

  Fly  1855 PKQQITIVHGSHTLGKPKKKSGSQRHSAGNIDYDNLSGSQEADDLPPSVGVVHYASGHTHGQQMQ 1919
            |.....:...|.|||  ::|....|.:   ||.|.:..::...|.|.|          .|.||
Zfish  1387 PAMNRAVSAPSRTLG--RRKLVRNRFT---IDTDIVFDTEPDSDSPDS----------QHAQQ-- 1434

  Fly  1920 PAGFRGRSKTVGDVTEITCSSPEPHQQQQQQQQQQQQLMQQGHAHAHPHPHPHPREPPPPPIRQP 1984
                     |:|.....|....|.....|:.::|:|.:.:...........|....|...|:..
Zfish  1435 ---------TIGVPAGDTDRWVEEQFDLQRYEEQEQDVKETDILSDDEDFCPSAMSPSTEPLLS- 1489

  Fly  1985 HLHHHSSDIERIDPGTKSEGEEDSQQGTIRPKATLGRTPNHLTLSTTS---TLSVGSTGSQARLI 2046
                 :..:|    |..:|.||::::...|...    ...|..||...   .:||.|...||
Zfish  1490 -----TLSLE----GDTAEEEEEAEEEDSRKDC----KSQHAKLSDVGKQCAMSVASVDEQA--- 1538

  Fly  2047 QSSHPPASYQPVLMKDLGKPSPDESELESESE 2078
                  .|...|.::   :.|..|:|.:.||:
Zfish  1539 ------TSDDVVWVR---RDSGAENETQDESQ 1561

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	67/125 (54%)
		PH	873..974	CDD:278594	52/101 (51%)
		TIAM1_RBD	1141..1219	CDD:176413	16/78 (21%)
		PDZ_signaling	1221..1308	CDD:238492	22/90 (24%)
		RhoGEF	1456..1647	CDD:238091	105/190 (55%)
		PH2_Tiam1_2	1650..1823	CDD:269957	72/179 (40%)
		PH	1693..1812	CDD:278594	47/120 (39%)
tiam1b	XP_009290217.1	PH1_Tiam1_2	387..513	CDD:269937	67/125 (54%)
		PH	400..502	CDD:278594	52/101 (51%)
		UBQ	721..796	CDD:294102	16/79 (20%)
		PDZ_signaling	799..879	CDD:238492	22/88 (25%)
		RhoGEF	1000..1189	CDD:214619	103/188 (55%)
		PH2_Tiam1_2	1191..1363	CDD:269957	70/174 (40%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	233	1.000	Domainoid score	I2328
eggNOG	1	0.900	-	-		E1_KOG3519
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002962
OrthoInspector	1	1.000	-	-		otm26125
orthoMCL	1	0.900	-	-		OOG6_105244
Panther	1	1.100	-	-	O	PTHR46001
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3224
SonicParanoid	1	1.000	-	-		X2782
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	11	10.800