DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and mcf2l2

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_021335410.1	Gene:	mcf2l2 / 100149630	ZFINID:	ZDB-GENE-100812-1	Length:	1284	Species:	Danio rerio

Alignment Length:	1493	Identity:	285/1493 - (19%)
Similarity:	489/1493 - (32%)	Gaps:	521/1493 - (34%)

- Green bases have known domain annotations that are detailed below.

  Fly   963 ENWVNSIHSACAAAFARHRGK-TGTLHL---LQEEIFRLEKAI--------ESDHKLK------- 1008
            :.|.::|     |:.||..|. .|.|.|   |:...| |::||        ..|.|||
Zfish   136 DKWNSAI-----ASLARIAGSFPGNLQLVLVLKPSRF-LQRAIADVSMRLHRDDFKLKVPIVMLN 194

  Fly  1009 HMAELQQSVVTDQET---------RHQIQTQILQWEENLERLHCEQF---------RLRCYMASL 1055
            .:::|...:...|.|         .|.      ||..:  |...|.|         .|:.:...|
Zfish   195 SLSDLHGYIDKGQLTCDLGGTLDYCHS------QWIHH--RTAIENFALTVKSTAQMLQQFGTDL 251

  Fly  1056 QSGELPNPKS-----LLTHVSRPTK------------NTLNKLGVFTVSSFHAFICARSPSLLNN 1103
            ...||||...     |:||..:..|            |||  |......:..|.....:|..:.|
Zfish   252 AETELPNDVQCTKDLLITHTEKHDKLKDELKLAVKQGNTL--LSCIKDQATKAESHILNPDEVEN 314

  Fly  1104 ------LLAGRGATKRRPPMLSRSNSGSSRRSMQMNSRDEPEKTFKVAMPDNAYSTVYLRD---- 1158
                  |||....|:.........:.....:.:|:...::..|..||::...:.:.:.|.|
Zfish   315 QTTVERLLAQLDETENAFEQFWSRHHLKLEQCLQLRHFEQDFKEVKVSLDGLSEALLGLSDTGDC 379

  Fly  1159 AMSVEEFLASACARRNLNPMEHFVRVKKRRDMEDHNYFVPHRNDLIENYLHNHEFVE----VCMK 1219
            ...||:.|.      .|..:|.    |.:..:|.......|.:.||::   ||..::    .|
Zfish   380 VARVEQLLG------ELKTLED----KAQECLEKAQLQAQHGDQLIQS---NHYAIDSIRPKC-- 429

  Fly  1220 ILYQVELQRTTLEQMWGFSVEA-----------ELIENAERQDELC-----CYVSRVEDKSVAMH 1268
                |||:|...:    ||.||           |:::..:..::.|     ...|:..|:....
Zfish   430 ----VELRRICDD----FSNEAKKKRDVLTKSLEILKRIDEVNQWCESGVYLLASQAVDRCQTQ- 485

  Fly  1269 NGIIKGDEIMVINGAIVSDLDMMYLESVLQEEQS------------LSMMMRSS-RTEPPDLVGI 1320
                :|.|      |.:.|:: .|:::..:::.|            ||.:::|. :|....|..:
Zfish   486 ----EGAE------AALQDIE-RYMKTAKEQQLSHLKELNNQYEVVLSELLKSKVQTALKTLNDV 539

  Fly  1321 MRVTDDMIDSL-------------VCPPPPTDPPVMSEEMITGLIVPAPGWNGTSKDLYSPEAES 1372
            ..:.:....||             |.|.|.:.|...|.:      :|.|    |....:....::
Zfish   540 QEMFEKRHASLKKLSTKQTRPVQPVAPRPESSPKRPSPK------IPHP----TPPTSHRKTTDN 594

  Fly  1373 SPATSFVDPAAMA----AQLAVGGVGGVVV--GGLGVAKPTSRTSSFEIENLLKTAEQETRKSSP 1431
            |........|.:|    .:.|.||:...|:  ||.|       .||..:.|      .||.:|.
Zfish   595 SSTNRQPSDAELAKRKNIRKAKGGIKIEVMHEGGQG-------GSSHILMN------NETEESL- 645

  Fly  1432 TGSVTSSVSTTALTPSRQLTDAEKLRKVVMELVDTERTYVKHLNNLLEHYLEPMKRETF------ 1490
                                 :.:...::.||::|||.||:.|.:::|.|...:.....
Zfish   646 ---------------------SNRRSHIMNELIETERLYVEELQSIIEGYAAALDDPELIYLIPP 689

  Fly  1491 -LSNAEINALFGNIHEIVTF-QRQFLQNLEESLDLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNH 1553
             |.|.: ..||||:.||..| |:.||:.||.|.: :|:.               :|:.||.....
Zfish   690 SLENKK-EVLFGNLPEIYQFHQKIFLKELENSAE-KPEL---------------VGTCFLKRKEE 737

  Fly  1554 FKLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPKQQHSSTLESYLIKPIQRILKYPLLLQQM 1618
            .::|..:|.:..:::.:......:...||.    ..:..|..:|::||:||:|||.||.|:|::|
Zfish   738 LQIYEKYCQNKPRSEALWRQCGDSMFFQEC----QKRLDHKLSLDAYLLKPVQRITKYQLMLKEM 798

  Fly  1619 RNLTDTRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQPI-------- 1675
                          .:..|..|..||       :.|....:.| :.:....|..|..
Zfish   799 --------------LKCSKNPEGTAE-------LEEAQATVLD-IIKSVNDSMHQIAITGFEGSL 841

  Fly  1676 -DLSPGDLLYYGGVE-WLNISDFLGKIK-----KGLELHAMCFVFKSAVVFLCKERLRQ---KKK 1730
             ||  |.||..|... |.:......|:|     |.::.|  .|::...::| ||:|...   .:|
Zfish   842 NDL--GKLLMQGSFNVWTDHKKGHSKVKDLARFKPMQRH--LFLYHKMILF-CKKREEMTDGHEK 901

  Fly  1731 LMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNS 1795
            ....|.|::            :.:..|.:..:...|:....:|         ....|:||::...
Zfish   902 SPSYSFKHS------------LKMGSVGITENVKGDIKKFEIW---------YNGREEVYIIQAP 945

  Fly  1796 TADFRNAFLKTIRQIIR---ESVRNMSIPMKNFGGSSGSVSGHSSQGMGSMGYPGNSQTLERPKQ 1857
            :.|.:|.::..||:::.   |:.|..|                                  :..|
Zfish   946 SMDVKNTWVSEIRKVLTSQLEACREAS----------------------------------QLHQ 976

  Fly  1858 QIT--IVHGSHTLGKPKKKSGSQRHSAGNIDYDNLSGSQEADDLPPSVGVVHYASGHTHGQQMQP 1920
            :||  |.|.      |.:   :.|.:|          .:::|...|..|.....||....|:
Zfish   977 KITENIYHA------PMR---NMRKTA----------LRQSDSSSPETGYRRSDSGPNMRQK--- 1019

  Fly  1921 AGFRGRSKTVGDVTEITCSSPEPHQQQQQQQQQQQQLMQQGHAHAHPHP-HPHPREPPPPPIRQP 1984
                 |:.|...:.....:.|            :::...||.::....| .|.|:|
Zfish  1020 -----RADTSSSLGSERAALP------------RKRFTLQGLSNRRSLPAEPAPKE--------- 1058

  Fly  1985 HLHHHSSDIERIDPGTKSEGEEDSQQGTIRPKATLGRTPNHLTLSTTSTLSVGSTGSQARLIQSS 2049
                  :::.|                    :.:|..|....|...|......|..|:...|:|.
Zfish  1059 ------TEVTR--------------------RFSLASTVGSATAPRTKAPLSSSLKSKRHQIKSD 1097

  Fly  2050 HPPASYQPVLMKDLGKPSPDESELESESEVHAAQVAASFNLSLGSSGSLFASDARDNQTQSRTQS 2114
            ..|..|:........|.......|.:|.:            |.||.||        .....|:.|
Zfish  1098 PTPFGYEGATKTRGFKKETMRDSLPAERK------------SKGSGGS--------KPAVPRSAS 1142

  Fly  2115 QIHSLHPSPRQSPRQSPRQSPKQDIEVIEIFESEAERLAASQQVAVVHQHPSDQARESGHKQRRD 2179
            |           |..|.|:.|..|.|..|...|.||.|:.|          || ..:.||.:..|
Zfish  1143 Q-----------PGWSQRRLPSMDAEDFETIPSSAEELSIS----------SD-GEDEGHNKNGD 1185

  Fly  2180 RDRNPTETDATARFMDKHLSDLEQENLTDGTCDIVAYMESLREKAFDPQDLAEDTGDH----QQN 2240
            .||                                 |...|..::...|||:.:.||.    :::
Zfish  1186 NDR---------------------------------YQVQLTYESCTAQDLSVEAGDFVQFLEES 1217

  Fly  2241 DSSL-----------SPEPQTIVENTQQ--------TVVVDIESPSRPATESDSTGNTTGPSE 2284
            ::.|           ...|.|::::|.:        .:..|:.:.:...||.|..|  |.|::
Zfish  1218 ENGLWLVKNLRTQKTGLVPPTVLQSTAEELDLYSNSDIFSDLCTAALTDTEDDIGG--TNPAQ 1278

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	6/22 (27%)
		PH	873..974	CDD:278594	2/10 (20%)
		TIAM1_RBD	1141..1219	CDD:176413	17/85 (20%)
		PDZ_signaling	1221..1308	CDD:238492	20/114 (18%)
		RhoGEF	1456..1647	CDD:238091	51/198 (26%)
		PH2_Tiam1_2	1650..1823	CDD:269957	36/193 (19%)
		PH	1693..1812	CDD:278594	22/126 (17%)
mcf2l2	XP_021335410.1	SEC14	77..217	CDD:214706	21/86 (24%)
		SPEC	348..554	CDD:238103	44/240 (18%)
		RhoGEF	649..826	CDD:238091	53/219 (24%)
		PH_Dbs	833..964	CDD:269934	29/156 (19%)
		Mating_C	932..>1122	CDD:315419	46/306 (15%)
		SH3_DBS	1188..1242	CDD:212791	11/86 (13%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910