DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and mcf2la

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_021332781.1	Gene:	mcf2la / 100148439	ZFINID:	ZDB-GENE-090312-122	Length:	1221	Species:	Danio rerio

Alignment Length:	1467	Identity:	283/1467 - (19%)
Similarity:	481/1467 - (32%)	Gaps:	479/1467 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly   797 IVQQASIESTDSRMCYLTSSEISDDDRMSLTTAVSDE-DDGESVMASP-------------YKAK 847
            |:||.|     |.:|   :::||.|.|.......... .:|..::..|             :...
Zfish    57 IMQQES-----SPLC---AADISPDLRKQFAFLSGGRGQNGSPIIIFPEYPEFGELEEQEFHNVL 113

  Fly   848 ATGTAASSFNCTGAVRKAGFLSVKKWLLRKKHQIELARKRGWKGYWVCLKGTTLLFYPCDSREGR 912
            ...|:..|.:.||    .||:.|          |:..:.|     |..:|||.|          |
Zfish   114 TYLTSIPSLSSTG----IGFILV----------IDRRQDR-----WASVKGTLL----------R 149

  Fly   913 SVEAAPKHLIIVDGAIMQP-----------IPEHPKRDY------IFCLSTAFGDAYLFQAPCQV 960
            ...:.|.:|.:|  .:::|           :.:..|.|:      |...|.....:|:.::....
Zfish   150 IAGSFPGNLQLV--LVLRPTTLFQRTISDILFKFNKDDFKMKVPVIMLSSVTELHSYIDRSQLTQ 212

  Fly   961 EL--------ENWVNSIHSACAAAFARHRGKTG-TLHLLQEEIFRLEKAIESDHKLKHMAELQQS 1016
            ||        |.|::  |......||....||. ||.....|:        ::.:|.:..|...:
Zfish   213 ELGGTQEYCHEKWIS--HRTAIEGFALMVKKTAQTLQSFGTEL--------AETELPNDVEATSN 267

  Fly  1017 VVTDQETRHQIQTQILQWEENLERLHCEQFRLRCYMASLQSGELPNPKSLLTHVSRPTKNTLNKL 1081
            ::|....:..      :.:|:|....|:..||   :.|:....:.:|...:.|
Zfish   268 LLTVHTEKKD------KMKEDLRIALCQGGRL---LESINEPLVKDPDYSMNH------------ 311

  Fly  1082 GVFTVSSFHAFICARSPSLLNNLLAGRGATKRRPPMLSRSNSGSSRRSMQMNSRDEPEKTFKVAM 1146
                             ..|.||     ||.:|                .:...||.|
Zfish   312 -----------------DELENL-----ATVQR----------------LLGQLDETE------- 331

  Fly  1147 PDNAYSTVYLRDAMSVEEFLASACARRNLNPMEHFVRVKKRRDMEDHNYFV-------------P 1198
              .|:...:.:....:|:.|          .:.||  .:..|::..|...|             |
Zfish   332 --TAFDDFWDKHQTKLEQCL----------QLRHF--EQNFREVRAHLDLVYDRLSGFSEVGISP 382

  Fly  1199 HRNDLIENYLHNHEFVEVCMKILYQVELQRTTLEQMWGFSVEA------ELIENAERQDELCCYV 1257
            ...:.|...|.|||  |...:::.|.....:..:|:    :||      .::........:|..:
Zfish   383 AHAEHILKELTNHE--ERASEVMDQALSLASDADQL----IEASHYAVDSILPKCSELRSVCEEI 441

  Fly  1258 SRVED-------KSVAMHNGIIKG----------------DEIMVINGAIVSDLDMMYLESVLQE 1299
            |.:..       |||.:|..:.|.                |:....:||          ||.|||
Zfish   442 STILRAKKAYLFKSVELHQCLEKATKWYDDGIYLLASQPVDKCQSQDGA----------ESALQE 496

  Fly  1300 -EQSLSMMMRSSRTEPPDLVGIMRVTD--------DMIDSLVCPPPPTDPPVMSEEMITGLIVPA 1355
             |:.|....:...|   ||.||.|..|        |.:|.:.      ...:..:||.
Zfish   497 IERYLDTANQHKLT---DLNGIWRDYDSVLTQDFRDQVDKVF------QKQLSMQEMF------- 545

  Fly  1356 PGWNGTSKDLYSPEAESSPATSFVDPAAMAAQLAVGGVGGVVVGGLGVAKPTSRTSSFE---IEN 1417
                  .|...|.:..::..|..|.|.|...:..:         ...|:.|..|..|.|   |..
Zfish   546 ------EKRRVSLKKLAAKQTRPVQPVAPRPEAII---------KTPVSSPAHREKSIEGDVING 595

  Fly  1418 LLKTAE--QETRKSSPTGSVTSSVSTTALTPSRQLTDAEKLRKVVM-ELVDTERTYVKHLNNLLE 1479
            ..|..|  .:...||...||:......|:           ||:.|| ||::|||.||:.|..:||
Zfish   596 NCKKGELHLQNEGSSRHPSVSDEEENFAV-----------LRRHVMNELLETERAYVEELLCVLE 649

  Fly  1480 HYLEPMKR--------ETFLSNAEINALFGNIHEIVTFQRQ-FLQNLEESLDLEPDFNKFEHCGQ 1535
            .|...|..        .|.|...:|  ||||:.||..|.:: ||:.||...|. |:.
Zfish   650 GYAAEMDNPAMSHLIPNTLLHKKDI--LFGNMSEIYQFHKKTFLRELEAYTDY-PEL-------- 703

  Fly  1536 FRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPKQQHSSTLESY 1600
                   :|..||..:...::|..:|.:..:::.:..........||.    ..|.:|...|:||
Zfish   704 -------VGQCFLERMTDLQIYEKYCQNKPRSESLWRQCSDCAFFQEC----QKKLEHKLGLDSY 757

  Fly  1601 LIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDHLFR 1665
            |:||:|||.||.|||:::...:.....|. .|.|||..:..:.:.:|:..           ||..
Zfish   758 LLKPVQRITKYQLLLKELLKYSKGCEGEE-DLQEALSSILGILKAVNDSM-----------HLIA 810

  Fly  1666 QHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKI------------KKGLELHAM--------- 1709
                            :..|.|    |:|| ||::            |||   ||.
Zfish   811 ----------------ITGYEG----NLSD-LGRLMMQGSFSVWTEHKKG---HAKVKDLARFKP 851

  Fly  1710 ----CFVFKSAVVFLCKERLRQ---KKKLMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSAKDM 1767
                .|:.:.|::| ||:|...   .:|....|.|::            :.::.|.:..::..|.
Zfish   852 MQRHLFLHEKALLF-CKKREENGEGYEKAPSYSFKHS------------LSMSAVGITENAKGDN 903

  Fly  1768 DSHFLWELIHLRSQLQRRSEKVYVLSNSTADFRNAFLKTIRQIIRESVRNMSIPMKNFGGSSGSV 1832
            ....:|         ....|:||::..||.:.:.|::..||:::...::......:.......|.
Zfish   904 KKFEIW---------CNSREEVYIVQASTPEIKTAWVNEIRKVLTRQLKACREASQQKSSDQISP 959

  Fly  1833 SGHSSQGMGSMGYPGNSQTL----ERPKQQITIVHGSHTLGKPKKKSGSQRHSAGNID------- 1886
            ...||.|:....:..|.::|    |:..:..|....|..|.|...|...:...:.|.:
Zfish   960 LTPSSTGLTLSPFKTNPKSLKKGDEKKTEPTTTDPSSAALFKNNDKLKDETVVSPNTERAAVAKK 1024

  Fly  1887 ------YDNLSGSQEADDLPPSVGVVHYASGHTHGQQMQPA--GFRGRSKTVGDVTEITCSSPEP 1943
                  :.||. ||:... |||       ..|...:|..|.  |||.....: .:|.:...|...
Zfish  1025 RFTLQGFSNLK-SQKGSS-PPS-------PDHKTKRQSDPTPFGFRDAGPHI-TLTRVRWLSTSS 1079

  Fly  1944 HQQQQQQQQQQQQLM------QQGHAHAHPHPHPHPREPPPPPIRQPHLHHHSSDIERIDPGTKS 2002
            ..|.:::...:..|.      ..|::.|....:..|.:               ...:::.||...
Zfish  1080 LLQTKRRGFTKASLSMDASEENDGYSSAEDPMNSDPED---------------EGGKKLSPGKYV 1129

  Fly  2003 EGEEDSQQGTIRPKATLGRTPNHLTLSTTSTLSVGSTGSQARLI-------QSSHPPASYQPVLM 2060
            ...:..:.|           |..||:.:..|:.:...|...|..       :....||:....|:
Zfish  1130 VVADHDKSG-----------PQELTVKSGDTVQLVREGDDGRWFVRNLRTSKEGWVPAANLLTLI 1183

  Fly  2061 KDLGKPSPDESELESESEVHAAQVAASFNLSLGSSGS 2097
                      .|.:|...:.:::.:.|.|||..||.|
Zfish  1184 ----------GESKSSQSLSSSEGSGSGNLSTSSSCS 1210

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	29/149 (19%)
		PH	873..974	CDD:278594	22/125 (18%)
		TIAM1_RBD	1141..1219	CDD:176413	15/90 (17%)
		PDZ_signaling	1221..1308	CDD:238492	21/116 (18%)
		RhoGEF	1456..1647	CDD:238091	60/200 (30%)
		PH2_Tiam1_2	1650..1823	CDD:269957	34/200 (17%)
		PH	1693..1812	CDD:278594	29/146 (20%)
mcf2la	XP_021332781.1	SEC14	73..219	CDD:214706	30/176 (17%)
		SPEC	386..552	CDD:321951	39/203 (19%)
		RhoGEF	626..803	CDD:238091	59/199 (30%)
		PH_Dbs	810..941	CDD:269934	32/176 (18%)
		SH3_DBS	1127..1181	CDD:212791	9/64 (14%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910