DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and itsn2

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_004918243.2	Gene:	itsn2 / 100127728	XenbaseID:	XB-GENE-1012238	Length:	1771	Species:	Xenopus tropicalis

Alignment Length:	1875	Identity:	312/1875 - (16%)
Similarity:	543/1875 - (28%)	Gaps:	701/1875 - (37%)

- Green bases have known domain annotations that are detailed below.

  Fly   393 TSTLKRM--LKPMPSTESPVTSPEMGRRRYNYYNANAAQTLGHPPHMHQ----HGMAMGMGGGGG 451
            |::|..|  |.|:|.: :|:. |.:|.........:.|.....|..:..    |..::.:.|||.
Frog   144 TASLTSMSSLPPLPMS-TPLV-PSLGSSSTLPNGTSTALLSSFPVPLSSSTLPHNSSINLLGGGF 206

  Fly   452 GGHHIMNNNTMGRASSQSSRFSGSRSSHEIGRGYPPRNLYLELE---------RER-----SCIE 502
            ....:....::...:|.||..|.:.|.    .|..|:..||:..         |::     .|:.
Frog   207 SSTTMQKTQSLIDLASSSSNSSSTTSL----TGNSPKTTYLDWAVPQASRLKYRQKFNSLDKCMT 267

  Fly   503 G---SPPSDNVMFDNQCYATTPSSSNGNSDQDQS------------YGQQQSSGQHPQQQQGPPQ 552
            |   .....|.:..:....|..::....:|.|:.            |....:....|.....||.
Frog   268 GYLTGSQVKNALVQSSLSHTQLATIWNLADIDKDGKLKADEFILAMYLTDMAKAGQPLPLTLPPD 332

  Fly   553 ------RSSRHQHHHQQAPNVTPTPGSPTSRLL-LEYEMHLRNTLAKGMDAESYSLHTFEALLSQ 610
                  ||           ||:.|...|.:..| |.::||      :....:..:..|||....:
Frog   333 LVPPAFRS-----------NVSITASDPINGTLPLYHQMH------EEEPPQKKTAGTFEEKRKE 380

  Fly   611 SME--NLEFAESLPGSNQRSPYPLRRPNAKSSTLPLPPHRPLSTIRDKERDRDRDGYYSDRNELI 673
            ::|  |:|.       .:|....|.:...::       .|.....|:::..|:|:....:|.:|:
Frog   381 NIEKGNMEL-------EKRRQVILEQQQREA-------ERKAQKQREEQERRERERQEQERKKLL 431

  Fly   674 -RERERERDRGYLSDHNSSFSNSRCASCIGESARAQWFRHSDGWRSGSSTIGSGSGHGMMTQQIP 737
             :||..||.|
Frog   432 EQERRMERQR------------------------------------------------------- 441

  Fly   738 GSGHKRSPWDSLPSLRQDSSLNDSGYKSARADSLEQRAEFIRQDSLRSEYLSDRESRYGIVQQAS 802
                      .|...|::                |:|.|..|:::.:.|....|:.....:::..
Frog   442 ----------ELERQREE----------------EKRKEIERREAAKQELERQRQQELERIRRQE 480

  Fly   803 IESTDSRMCYLTSSEISDDDRMSLTTAVSDEDDGESVMASPYKAKATGTAASSFNCTGAVRKAGF 867
            :.:..:|                         :.|.:|....|.|:
Frog   481 LFNQRNR-------------------------EQEEIMKLKSKKKS------------------- 501

  Fly   868 LSVKKWLLRKKHQIELAR---KRGWKGYWVCLKGTTL--LFYPCDSREGRSVEAAPKHLIIVDGA 927
            |.::...|..|||...:|   .||.|.    ||.|.|  |...||:.            |:....
Frog   502 LQLELEALEDKHQQITSRVQDVRGKKQ----LKRTELETLDRKCDAG------------IMEIKQ 550

  Fly   928 IMQPIPEHPKRDYIFCLSTAFGDAYLFQAPCQVELEN-----WVNSIHSACAAAFARHRGKTGTL 987
            :.|.:.|:..:..|......|.:..:.|  .|..:.|     .....|...:.       |.|..
Frog   551 LQQELQEYQNKLSILLPEKQFLNEKINQ--MQAVISNDKSGTGFQGFHKKVSE-------KDGLC 606

  Fly   988 HLLQEEIFRLEKAIESDHKLKHM-------AELQQSVVTDQETRHQI-----------------Q 1028
            ..|:|::..|||  |:..||:.|       .||:.|....|...||:                 |
Frog   607 QRLREQLEALEK--ETSSKLQEMDSFNNQLKELRGSYSKQQSALHQLQKIKQEKLREAEQKRAEQ 669

  Fly  1029 TQILQWEENLERLHCEQFRLRCYMASLQSGELPNPKSLLTHVSRPTKNTLNKLGVFTVSSFHAFI 1093
            .|..|.||...| ..:|.:...::.:|:..|....:.|.....|..|
Frog   670 AQKKQEEEEAAR-RAKQEKENLWLENLKKEEEEKQQRLEDERRREQK------------------ 715

  Fly  1094 CARSPSLLNNLLAGRGAT--------------------------------KRRPPML-------S 1119
                  ||....|...||                                |||...|       |
Frog   716 ------LLEKQKAEEEATARQREAQQRLAEEKKRQAEREEEQRKKQQEEEKRRQAQLKKDLEEKS 774

  Fly  1120 RSNSGSSRRSMQMNSRDEPEKTFKVAMPD------------NA---------------------- 1150
            ::.....||......:|..::..|:::.:            ||
Frog   775 KAEDEERRRKESAGRKDSEKRISKLSLDEKFADKLKNIGGRNASSERGREANTTSVSDNRRSSAA 839

  Fly  1151 ----YSTVYLRDAMSVEEFLASA-------------------CARRNL----------------- 1175
                |:.:|..:|.:.:|...:|                   |.|.|:
Frog   840 AFVNYTALYPFEARNADELSFNAGDILQVDEKNIGEPGWLYGCLRGNVGWFPSNYAEKATEIEAP 904

  Fly  1176 -----------------NPMEHFVRVKKRRDMEDHNYFVPHRNDLIENYLHNHEFVEVCMKILYQ 1223
                             :|.:..:..|...:::.....:|..:..:.|
Frog   905 LSPKKALLPPTLSVSTSSPAKPLLTSKSSSELDTEYQNLPFSSLNVNN----------------- 952

  Fly  1224 VELQRT----------TLEQMWGFSVEAELIENAERQDELCCYVSRVEDKSVAMHNGIIKGDEIM 1278
            ...|:|          |:..|.|.....|.:    :...||.:.::.::     |....|.|.|:
Frog   953 TTWQKTSAFTRTVSPGTISPMHGQGQPGESV----KAQALCSWTAKKDN-----HLNFSKNDTIV 1008

  Fly  1279 VINGAIVSDLDMMYLESVLQEEQSLSMMMRSSRTE----PPDLVGIMRVTDDMIDSLVCPPP--- 1336
            |:                   ||..:......|.:    |...|.|:..||.....   |.|
Frog  1009 VL-------------------EQQENWWFGEVRGQKGWFPKSYVKILPGTDAKTPE---PEPVYA 1051

  Fly  1337 -----PTDPPVMSEEMIT----------------G---LIVPAPG--WNGTSKD----------- 1364
                 |..|....||.:.                |   |:....|  |.|..:|
Frog  1052 AVKKKPVAPSAAGEEYVALYSYSSSEPGDLIFNEGDLILVTQKDGEWWTGRVEDRTGIFPSNYVR 1116

  Fly  1365 ----------------LYSPEAESSPATSFVDPAAMAAQLAVGGV--------GGVVVGGLGVAK 1405
                            ...||. :...|::.........||.|.:        .|...|.| .|:
Frog  1117 TRDQEVSSGAGKTGTLTKKPEI-AQVTTAYTATGTEQLSLAPGQLILIQKKNPSGWWQGEL-QAR 1179

  Fly  1406 PTSRTSSFEIENLLKTAEQETRKSSP--------------------------------------- 1431
            ...|...:...:.:|.......|::|
Frog  1180 GKKRQKGWFPASHVKLLGPNNEKAAPAFIPVCQVIAMYDYIANNEDELHFGKGQLINVLNKDDVD 1244

  Fly  1432 ---------TGSVTSSV--STTALTPSRQ-----------LTDAEKLRKVVMELVDTERTYVKHL 1474
                     ||...|:.  .||...||:|           |....|.:..:.||:.||..|:..|
Frog  1245 WWQGEINGVTGLFPSNYVKMTTESDPSQQWCADLHTLDTMLPMERKRQGYIHELIQTEERYMDDL 1309

  Fly  1475 NNLLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFLQNLEESLDLEPDFNKFEHCGQFRNV 1539
            ..::|.:.:||.....|:..|:..:|.|..|::....:.|:.|...          :..|..:..
Frog  1310 QLVIEVFQKPMAESGLLTEGEMGMIFVNWKELIMSNTKLLKALRVR----------KKTGGEKMP 1364

  Fly  1540 LFAIGSAFLYYVNHFKLYSSFCASHSKAQKVL-HPNEGNHALQEFL--AARNPKQQHSSTLESYL 1601
            :..||......::|...|..||:.......:| |..:.:...:::|  .|.:.:.: ...|.|:|
Frog  1365 VQMIGDILATELSHMPAYIRFCSCQLNGAALLQHKTDEDADFKDYLKRLAMDSRCK-GMPLSSFL 1428

  Fly  1602 IKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQRI------------HE 1654
            :||:|||.:|||:::.:...|.....:|.:|..||...|::...:||..|.            |.
Frog  1429 LKPMQRITRYPLIIKNILENTPESLPDHSNLKFALDRAEELCSQVNEGVREKENSDKLEWIQGHV 1493

  Fly  1655 EYGAIFDHLFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAVVF 1719
            :...:.:.:......:|     |.|..||:.|.|.         |.|...||:...|.....:.:
Frog  1494 QCEGLTEQIIFNSLTNC-----LGPRKLLHSGKVY---------KTKSNKELYGFLFNDFLLLTY 1544

  Fly  1720 LCKERLRQ-KKKLMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQ 1783
            :.|:.:.. ..||.   |...|.:.::  |:..:.:.||.|:..|....|.. ::.:.|:
Frog  1545 MVKQFVSSGSDKLF---SPKTTAQYKM--YKTPVFLNEVLVKLPSDPSSDEP-VFHISHI----- 1598

  Fly  1784 RRSEKVYVLSNSTADFRNAFLKTIRQIIRESVRNMSIPMKNFGGSSGSVSGHSSQGMGSMGYPGN 1848
               ::||.|.....:.|.|::    |.|:.:..|.....|.....:.......|.|:|.:
Frog  1599 ---DRVYTLRTDNINERTAWV----QKIKSASENFIDTEKKKRERAYQARSQKSSGIGRL----- 1651

  Fly  1849 SQTLERPKQQITIVHGSHT----LGKPKKKS--------GSQRHSAGNIDYDNLS 1891
                        :||....    ..||..||        |||.::...:. |.||
Frog  1652 ------------LVHVIEATELKASKPNGKSNPYCEVSMGSQSYTTRTLT-DTLS 1693

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	26/134 (19%)
		PH	873..974	CDD:278594	24/110 (22%)
		TIAM1_RBD	1141..1219	CDD:176413	15/168 (9%)
		PDZ_signaling	1221..1308	CDD:238492	15/96 (16%)
		RhoGEF	1456..1647	CDD:238091	44/193 (23%)
		PH2_Tiam1_2	1650..1823	CDD:269957	34/185 (18%)
		PH	1693..1812	CDD:278594	23/119 (19%)
itsn2	XP_004918243.2	EH	19..111	CDD:197477
		EH	244..323	CDD:197477	8/78 (10%)
		PTZ00121	<351..795	CDD:173412	103/640 (16%)
		tolA_full	617..>823	CDD:274303	40/232 (17%)
		SH3_Intersectin2_1	841..897	CDD:212921	8/55 (15%)
		SH3_Intersectin2_2	984..1035	CDD:212923	12/74 (16%)
		SH3_Intersectin2_3	1066..1117	CDD:212925	7/50 (14%)
		SH3_Intersectin2_4	1138..1196	CDD:212927	10/59 (17%)
		SH3_Intersectin2_5	1211..1264	CDD:212929	3/52 (6%)
		RhoGEF	1291..1474	CDD:238091	44/193 (23%)
		PH_13	1493..1635	CDD:406942	33/173 (19%)
		C2_Intersectin	1635..1769	CDD:176021	15/77 (19%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910