Protein Alignment Ppn and APP

DIOPT Version :10

• Sequence 1: NP_788752.2
• Gene: Ppn / 43872
• FlyBaseID: FBgn0003137
• Length: 2898
• Species: Drosophila melanogaster
• Sequence 2: NP_000475.1
• Gene: APP / 351
• HGNCID: 620
• Length: 770
• Species: Homo sapiens
• Alignment Length: 382
• Identity: 87/382 - (22%)
• Similarity: 128/382 - (33%)
• Gaps: 119/382 - (31%)

  Fly  1706 GTNNRFDS----LEQCQGTCAASE--NLPTCEQPVESGPCAGNFERWYYDNETDICRPFTYGGCK 1764
            ||....|:    |:.||......:  |:....|||       ..:.|                ||
Human    58 GTKTCIDTKEGILQYCQEVYPELQITNVVEANQPV-------TIQNW----------------CK 99

  Fly  1765 GNKNNYPT--EHACNYNC------RQPGVLKDRCA-LPKQTGDCSEKLAKWHFSESEKRCVPFYY 1820
            ..:....|  .....|.|      ....::.|:|. |.::..|..|....|| :.:::.|
Human   100 RGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWH-TVAKETC----- 158

  Fly  1821 SGCGGNKNNFPTLESCEDHCPRQVAKDICEIPAEVGECANYVTSWYYDTQDQACRQFYYGG---- 1881
            |....|.:::..|..|.....|.|....|.:..|    ::.|.|...:..|.   ..::||
Human   159 SEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEE----SDNVDSADAEEDDS---DVWWGGADTD 216

  Fly  1882 -CGGNENRF--PTEESCLARCDRK------------------PEP-------------TTTTPAT 1912
             ..|:|::.  ..||..:|..:.:                  .||             ||||...
Human   217 YADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTE 281

  Fly  1913 RPQPSRQDVCDEEPAPGECSTWVLKWHFDRKIGACRQFYYGNCGGNGNRFETENDCQQRC----- 1972
            ..:...::||.|:...|.|...:.:|:||...|.|..|:||.||||.|.|:||..|...|
Human   282 SVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMS 346

  Fly  1973 -----LSQEPPAPTPPRAPAPTRQPDPAPTVAQCSQPADPGQCDKWALHWNYNETEG 2024
                 .:|||.|          |.|...||.|    .:.|...||      |.||.|
Human   347 QSLLKTTQEPLA----------RDPVKLPTTA----ASTPDAVDK------YLETPG 383

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ppn	NP_788752.2	TSP1	60..111	CDD:214559
		ADAMTS_CR_3	116..213	CDD:437068
		ADAMTS_spacer1	222..329	CDD:461796
		TSP1_ADAMTS	347..396	CDD:465950
		TSP1_ADAMTS	400..457	CDD:465950
		TSP1_ADAMTS	465..520	CDD:465950
		TSP1_ADAMTS	580..632	CDD:465950
		TSP1_ADAMTS	643..693	CDD:465950
		MSCRAMM_ClfA	<1049..1262	CDD:468110
		Kunitz_papilin_lacunin-like	1612..1663	CDD:438681
		Kunitz_BPTI	1670..1721	CDD:425421	6/18 (33%)
		Kunitz-type	1730..1780	CDD:438633	8/51 (16%)
		Kunitz_BPTI	1789..1840	CDD:425421	11/51 (22%)
		Kunitz_papilin_mig6-like	1849..1899	CDD:438679	12/56 (21%)
		Kunitz_papilin_mig6-like	1922..1972	CDD:438679	21/49 (43%)
		Kunitz_papilin_mig6-like	2001..2051	CDD:438679	7/24 (29%)
		Kunitz-type	2071..2121	CDD:438633
		Kunitz_BPTI	2127..2178	CDD:425421
		Kunitz_BPTI	2193..2245	CDD:425421
		Kunitz_BPTI	2252..2303	CDD:425421
		Kunitz_BPTI	2317..2372	CDD:425421
		WAP	2455..2497	CDD:459672
		Ig	2530..2610	CDD:472250
		Ig strand B	2539..2543	CDD:409353
		Ig strand C	2552..2556	CDD:409353
		Ig strand E	2576..2579	CDD:409353
		Ig strand F	2589..2594	CDD:409353
		Ig strand G	2603..2606	CDD:409353
		IG_like	2627..2703	CDD:214653
		Ig strand B	2636..2640	CDD:409353
		Ig strand C	2649..2653	CDD:409353
		Ig strand E	2670..2674	CDD:409353
		Ig strand F	2684..2689	CDD:409353
		Ig strand G	2697..2700	CDD:409353
		I-set	2766..2845	CDD:400151
		Ig strand B	2771..2775	CDD:409353
		Ig strand C	2784..2788	CDD:409353
		Ig strand F	2821..2826	CDD:409353
		PLAC	2851..2883	CDD:462560
APP	NP_000475.1	A4_EXTRA	24..188	CDD:128326	30/158 (19%)
		GFLD subdomain. /evidence=ECO:0000255|PROSITE-ProRule:PRU01217	28..123		16/87 (18%)
		CuBD subdomain. /evidence=ECO:0000255|PROSITE-ProRule:PRU01217	131..189		15/63 (24%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	194..284		16/92 (17%)
		Kunitz_ABPP-like	294..341	CDD:438650	19/46 (41%)
		OX-2. /evidence=ECO:0000269|PubMed:2649245	344..365		6/30 (20%)
		APP_E2	366..548	CDD:463752	9/28 (32%)
		Heparin-binding	391..423
		Heparin-binding	491..522
		Collagen-binding. /evidence=ECO:0000269|PubMed:8576160	523..540
		JMTM_Notch_APP	693..767	CDD:425406
		Interaction with PSEN1. /evidence=ECO:0000269|PubMed:30630874	695..722
		Basolateral sorting signal	724..734
		Interaction with G(o)-alpha	732..751
		Required for the interaction with KIF5B and for anterograde transport in axons. /evidence=ECO:0000269|PubMed:17062754	756..770
		YENPXY motif, contains endocytosis signal. /evidence=ECO:0000269|PubMed:10383380	757..762