DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment nej and ep300

DIOPT Version :9

Sequence 1:	NP_001188575.1	Gene:	nej / 43856	FlyBaseID:	FBgn0261617	Length:	3282	Species:	Drosophila melanogaster
Sequence 2:	XP_004913855.2	Gene:	ep300 / 734023	XenbaseID:	XB-GENE-482293	Length:	2492	Species:	Xenopus tropicalis

Alignment Length:	3421	Identity:	1190/3421 - (34%)
Similarity:	1484/3421 - (43%)	Gaps:	1126/3421 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 MADHLDE---PPQKRVKMDPTDIS----------YFLEENLPDELVSSNSGWSDQLTGGAGGGNG 53
            ||::|.:   |..|:.||....:|          :.||::|||||::|:.          .|...
Frog     1 MAENLVDPGPPSAKKPKMSSPSLSSSDVPDFGSLFDLEQDLPDELINSSE----------FGLTN 55

  Fly    54 GGGASGVTTNPTSGPNPGGGPNKPAAQGPGSGTGGVGVGVNVGVGGVVGVGVVPSQMNGAGGGNG 118
            ||..|.:..:                                       :.:||.
Frog    56 GGDLSQLHNS---------------------------------------MNMVPD---------- 71

  Fly   119 SGTGGDDGSGNGSGAGNRISQMQHQQLQHLLQ-------QQQQGQKGAMVVPGMQQLGS--KSPN 174
                               :..:|:||..||:       ....||:.:...||:..|.|  |||
Frog    72 -------------------AAAKHKQLSELLRGGTTSNMSMGLGQQTSQNSPGINLLNSMVKSP- 116

  Fly   175 LQSPNQGGMQQVVGTQMGMVNSMPMSISNNGNNGMNAIPGMNTIAQGNLGNMVLTNSVGGGMGGM 239
               .||||:           .|..|.:..:||.|..|.....|.|.|.:
Frog   117 ---MNQGGL-----------TSPNMGMGGSGNTGPTAQQINPTSAAGMI---------------- 151

  Fly   240 VNHLKQQPGGGGGGMINSVSVPGGPGAGAGGVGAGGGGAVAANQGMHMQNGPMMGRMVGQQHMLR 304
                   ||      :||.....|.|..||.:.||.|                       |.|::
Frog   152 -------PG------VNSPVNQHGMGINAGMMTAGNG-----------------------QSMIQ 180

  Fly   305 GPHLMGASGGAGGPGNGPGGGGPRMQ--NPNMQMNFRPFAAQLNSLPYGVGQYGGPGGGNNPQQQ 367
            |..:.|:.|.        |.|.|.||  ||||.......|..|...|    |.||..|...||..
Frog   181 GQMMNGSVGS--------GRGRPNMQYPNPNMGNTGNILAETLQGGP----QIGGQPGMRGPQPT 233

  Fly   368 QQQQQQQLLAQQMAQRGGVVPGMPQGNRPVGT-----VVPMSTLGGDGSGPAGQLVSGNPQQQQM 427
            ...:.                |:.....|.||     |||.  :||        .|.|: |.|..
Frog   234 AMNKM----------------GLMNNPNPYGTPYGQNVVPQ--MGG--------AVMGS-QIQNK 271

  Fly   428 LAQQQTGAMGPRPPQPNQLLGHPGQQQQQQQQPGTSQQQQQQQGVGIGGAGVVANAGTVAGVPAV 492
            .|.|.:.:..|...:|.  :|.|...||      ||.|.||              .|.|...|..
Frog   272 AALQNSLSQFPMDKKPG--VGMPNMNQQ------TSTQVQQ--------------TGMVTAAPPS 314

  Fly   493 AGGGAGGAVQSSGPGGANRDVPDDRKRQIQQQLMLLLHAHKCNRRENLNPNREVCNVNYCKAMKS 557
            .|   .||.|..|.|....| |:.|| .|||||:||||||||.|||..|.....||:.:|:.||:
Frog   315 MG---AGAPQGIGSGAPTAD-PEKRK-LIQQQLVLLLHAHKCQRREQANGEVRQCNLPHCRTMKN 374

  Fly   558 VLAHMGTCKQSKDCTMQHCASSRQILLHYKTCQNSGCVICYPF-----RQNHSVFQNANVPPGGG 617
            ||.||..|:..|.|.:.||||||||:.|:|.|....|.:|.|.     ::|.::.        ||
Frog   375 VLNHMTHCQAGKSCQVAHCASSRQIISHWKNCTRHDCPVCLPLKNAGDKRNQTLL--------GG 431

  Fly   618 PAGIGGAPPGGGGAGGGAAGAGGNLQQQQQQQQQQQQNQQPNLTGLVVDGKQGQQVAPGGGQNTA 682
            |....|||        ||.|.                              .|||.||.....:.
Frog   432 PPVTLGAP--------GAVGT------------------------------VGQQPAPSINATSQ 458

  Fly   683 IVLPQQQGAGGAPGAPKTPADMVQQLTQQQQQQQQQVHQQQVQQQELRRFDGMSQQVVAGGMQQQ 747
            |.....:.|..|.|.|       .|..|.|.|.|.||..||         .|.|.|    ||:..
Frog   459 IDPSSIERAYAALGLP-------YQGNQMQTQTQAQVKGQQ---------SGQSPQ----GMRSL 503

  Fly   748 QQQGLPPVIRIQGAQPAVRVLGPGGPGGPSGPNVLPNDVNSLHQQQQQMLQQQQQQGQNRRRGGL 812
                                                |.:||
Frog   504 ------------------------------------NTMNS------------------------ 508

  Fly   813 ATMVEQQQQHQQQQQQPNPAQLGGNIPAPLSVNVGGFGNTNFGGAAAGGAVGANDKQQLKVAQVH 877
            :||
Frog   509 STM-------------------------------------------------------------- 511

  Fly   878 PQSHGVGAGGASAGAGASGGQVAAGSSVLMPADTTGSG-NAGNPNQNAGGVAGGAGGGNGGNTGP 941
                           |.:|| |...:|.|:|.....|| ||.:|..|..|  |....|:.....|
Frog   512 ---------------GVNGG-VGVQASSLLPDSMLHSGMNAQSPMMNESG--GVPNLGSMPTAAP 558

  Fly   942 PGDN--EKDWRESVTADLRNHLVHKLVQAIFPTSDPTTMQDKRMHNLVSYAEKVEKDMYEMAKSR 1004
            |..:  .|.|.|.:|.|||||||||||||||||.||..::|:||.|||:||.|||.||||.|.||
Frog   559 PSSSGIRKPWHEDITQDLRNHLVHKLVQAIFPTPDPAALKDRRMENLVAYARKVEGDMYESANSR 623

  Fly  1005 SEYYHLLAEKIYKIQKELEEKRLKR-KEQH---------QQMLMQQQGVANPVAGGAAGG----- 1054
            :||||.||||||||||||||||..| ::||         ...:.|.||:|.|..|.:..|
Frog   624 AEYYHFLAEKIYKIQKELEEKRRTRLQKQHLIPTPPGMPPTQMNQNQGMAQPQTGLSTNGPLPDP 688

  Fly  1055 -AGSAAGVAGGVVLPQQQQQQQQQQQQQGQQPLQSCIHPSISPMGGVMPPQQLRPQGPPGILGQQ 1118
             :...|.|...::...|.|....|..|.|.| :||            |..:|..|...||.:||
Frog   689 NSMMRANVPNQIMNRMQTQTGMNQFGQMGMQ-MQS------------MGQRQQAPLQHPGQMGQ- 739

  Fly  1119 TAAGLGVGVGVTNNM-VTMRSHSPGGNMLALQQQQRMQFPQQQQQQPPGSGAGKMLVGPPGPSPG 1182
                    .|..|.| ...|...||    .:||..:.||..|.|.|     |...::...|.:.|
Frog   740 --------TGPMNQMGFQARMQQPG----TMQQTAQNQFLSQNQFQ-----ASSPVMNTGGMNTG 787

  Fly  1183 GMVVNPALSPYQTTNVLTSPVPGQQQQQQFINANGGTGANPQLSEIMKQRHIHQQQQQQQQQQQQ 1247
            |:          ..:.:..|.||.|              ||.
Frog   788 GL----------NASGMPMPQPGNQ--------------NPS----------------------- 805

  Fly  1248 GMLLPQSPFSNSTPLQQQQQQQQQQQQQQQATSNSFSSPMQQQQQGQQQQQQKPGSVLNNMPPTP 1312
                .|:|..|                    .|:..|||:                    |||
Frog   806 ----SQAPMPN--------------------PSHPVSSPV--------------------MPP-- 824

  Fly  1313 TSLEALNAGAGAPGTGGSASN----VTVSAPSPSPGFLSNGPSIGTPSNNNNNSSANNNP----- 1368
                      ||.|.....|:    :..::|||:|.        .||:.::........|
Frog   825 ----------GAAGNQMHCSSHVQPMHRNSPSPAPS--------RTPTPHHTPPGLTPQPTQTPV 871

  Fly  1369 ----PSVSSLMQQPLSNRPG-TPPYIPAS---PVPATSASGLAASSTPASAAATCASSGSGSNSS 1425
                |:.:.:.|.||.:|.. |||.:..|   |.|..|.:....::.|
Frog   872 QPILPTAAPISQAPLQHRESPTPPQVTPSLSIPTPVMSTADQQIATPP----------------- 919

  Fly  1426 SGATAAGASSTSSSSSAGSGTPL------SSVSTPTSATMATSSGGGGGGGGNAGGGSSTTPASN 1484
                    |...|...:....||      .|::||.:                       ||.:.
Frog   920 --------SQQPSQQFSSQQQPLPRPPSQPSLTTPVA-----------------------TPTAP 953

  Fly  1485 PLLLMSGGTAGGGTGATTTTSTSSSSRMMSSSSSLSSQMAALEAAARDNDDETPSPSGENTNGSG 1549
            ||.:.    ....|..|......||:...::|..:|||....|          |.|
Frog   954 PLPMQ----LQPSTPPTANLDCPSSNPPSTNSPEVSSQPPVQE----------PPP--------- 995

  Fly  1550 GSGNAGGMASKGKLDSIKQDDDIKKEFMDDSCGGNNDSSQMDCSTGGGKGKNVNNDGTSMIKMEI 1614
                     .:.|::..::|||::.|.|:.                           .:..|.|:
Frog   996 ---------QEVKMEIKQEDDDLEPEPMEP---------------------------PAEEKPEV 1024

  Fly  1615 KTEDGLDGEVKIKTEAMDVDEAGGSTAGEHHGEGGGGSGVGGGKDNINGAHDGGATGGAVDIKPK 1679
            |.|..::   :.|:|..:::|.                     |:.:.           .:||.:
Frog  1025 KAEVAVE---ECKSEPAELEEK---------------------KEEVK-----------TEIKEE 1054

  Fly  1680 TETKPLVP----EPLAPNAGDKKKKCQFNPEELRTALLPTLEKLYRQEPESVPFRYPVDPQALGI 1740
            .| ||..|    .|..|  |..|||. |.|||||.||:||||.||||:|||:|||.|||||.|||
Frog  1055 DE-KPATPAAQTSPAPP--GQSKKKI-FKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGI 1115

  Fly  1741 P-------DYFEIVKKPMDLGTIRTNIQNGKYSDPWEYVDDVWLMFDNAWLYNRKTSRVYRYCTK 1798
            |       |||||||.||||.||:..:..|:|.:||:||:|:||||:|||||||||||||:||:|
Frog  1116 PKNTSVLQDYFEIVKTPMDLSTIKRKLDTGQYQEPWQYVEDIWLMFNNAWLYNRKTSRVYKYCSK 1180

  Fly  1799 LSEVFEAEIDPVMQALGYCCGRKYTFNPQVLCCYGKQLCTIPRDAKYYSYQNSLKEYGVASNRYT 1863
            |:||||.|||||||:||||||||..|:||.|||||||||||||||.|||||||..:||:.::||.
Frog  1181 LAEVFEQEIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNSSPKYGLLADRYH 1245

  Fly  1864 YCQKCFNDIQGDTVTLGDDPLQSQTQIKKDQFKEMKNDHLELEPFVNCQECGRKQHQICVLWLDS 1928
            :|:||||:|||:.|:|||||.|.||.|.||||.:.|||.|:.|.:|.|.|||||.||||||..::
Frog  1246 FCEKCFNEIQGECVSLGDDPTQPQTTISKDQFSKRKNDTLDPEQYVECLECGRKMHQICVLHNET 1310

  Fly  1929 IWPGGFVCDNCLKKKNSKRKENKFNAKRLPTTKLGVYIETRVNNFLKKK-EAGAGEVHIRVVSSS 1992
            |||.||||:.||||....||||||:|||||||:||.::|.|||:||::: ...||||.||||.:|
Frog  1311 IWPTGFVCEGCLKKTGRNRKENKFSAKRLPTTRLGSFLENRVNDFLRRQNNPEAGEVIIRVVHAS 1375

  Fly  1993 DKCVEVKPGMRRRFVEQGEMMNEFPYRAKALFAFEEVDGIDVCFFGMHVQEYGSECPAPNTRRVY 2057
            ||.|||||||:.:||:.|||...||||.|||||||||||:::||||||||||||:||.||.||||
Frog  1376 DKTVEVKPGMKAKFVDTGEMAESFPYRTKALFAFEEVDGVELCFFGMHVQEYGSDCPQPNQRRVY 1440

  Fly  2058 IAYLDSVHFFRPRQYRTAVYHEILLGYMDYVKQLGYTMAHIWACPPSEGDDYIFHCHPTDQKIPK 2122
            |:||||||||||:..|||||||||:||::|||:||||..|||||||||||||||||||.||||||
Frog  1441 ISYLDSVHFFRPKNLRTAVYHEILIGYLEYVKRLGYTAGHIWACPPSEGDDYIFHCHPADQKIPK 1505

  Fly  2123 PKRLQEWYKKMLDKGMIERIIQDYKDILKQAMEDKLGSAAELPYFEGDFWPNVLEESIKELDQEE 2187
            ||||||||||||||.:.||:|.|||||.|||.||:|.||.|||||||||||||||||||||:|||
Frog  1506 PKRLQEWYKKMLDKSVSERVIHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEE 1570

  Fly  2188 EEKRKQAEAAEAAAAANLFSIEENEVSGDGKKKGQKKAKKSNKSKAA-QRKNSKKSNEHQSGNDL 2251
            ||::::...:..       |||..:  ||.|...:|..||::|:|:: .|.:.||.......|||
Frog  1571 EERKREENTSNE-------SIEVGK--GDSKNAKKKNNKKTSKNKSSLSRGSKKKPGMPNVSNDL 1626

  Fly  2252 STKIYATMEKHKEVFFVIRLHSAQSAASLAPIQDPDPLLTCDLMDGRDAFLTLARDKHFEFSSLR 2316
            |.|:||||||||||||||||.:..:|.||.||.:||||:.||||||||||||||||||.||||||
Frog  1627 SQKLYATMEKHKEVFFVIRLSAGPAANSLPPIVEPDPLIPCDLMDGRDAFLTLARDKHLEFSSLR 1691

  Fly  2317 RAQFSTLSMLYELHNQGQDKFVYTCNHCKTAVETRYHCTVCDDFDLCIVCKEKVGHQHKMEKLGF 2381
            ||::||:.||.|||.|.||:||||||.||..||||:|||||:|:||||.|.....|:|||||||.
Frog  1692 RAKWSTMCMLVELHTQSQDRFVYTCNECKHHVETRWHCTVCEDYDLCITCYNTKTHEHKMEKLGL 1756

  Fly  2382 DIDDGSALADHKQA----NPQEARKQSIQRCIQSLAHACQCRDANCRLPSCQKMKLVVQHTKNCK 2442
            .:||.|   :::||    :|.::|:.|||||||||.||||||:|||.||||||||.||||||.||
Frog  1757 GLDDES---NNQQAAATQSPGDSRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCK 1818

  Fly  2443 RKPNGGCPICKQLIALCCYHAKNCEEQKCPVPFCPNIKHKLKQQQSQQKFQQQQLLRRRVALMSR 2507
            ||.|||||||||||||||||||:|:|.|||||||.|||.||:|||.|.:.||.|:||||:|.|.|
Frog  1819 RKTNGGCPICKQLIALCCYHAKHCQENKCPVPFCLNIKQKLRQQQLQHRLQQAQMLRRRMASMQR 1883

  Fly  2508 TAAPAALQG-PAAVS-GPTVVSGGVPVVGMSGVAVSQQVIPGQAGILPPGAGGMSPSTVAVPSPV 2570
            .......|| |:..| ||...:|..|....:.....|.  |.|....||   .|.|.::|...|.
Frog  1884 AGGVGPQQGLPSPNSAGPATPTGQQPPTPQTPQPPPQP--PTQQQPPPP---NMPPYSMARAEPA 1943

  Fly  2571 SGGAGAGGMGGMTSPHPHQPGIGMKPGGGHSPSPNVLQVVKQVQEEAARQ-QVSHGGGFGKGVPM 2634
            .|.:.....|.||.|.|.|.|...:...|  |.|..:::..|:|..|..| |::....|.:  ||
Frog  1944 RGISQGKAAGQMTPPTPPQAGAMQQQVQG--PPPAAVEMAMQIQRAAETQRQMAKAQIFPR--PM 2004

  Fly  2635 APPVMNRPMGGAGPNQNVVNQLGGMGVGVGGVGGVGVGGVGGVGVNQLNSGGGNTPGAPISGPGM 2699
            ..|...:||....|                            :|:|            |..||..
Frog  2005 MNPHQMQPMNPMQP----------------------------MGMN------------PQQGPRG 2029

  Fly  2700 NVNHLMSMDQWGGGGAGGGGANPGGGNPQARYANNTGGMRQPTHVMQTNLIPPQQQQQMMGGLGG 2764
            .| |:.|            |..||...||.:        :||         ||.|.|......|.
Frog  2030 QV-HMES------------GIGPGMQQPQQQ--------QQP---------PPTQPQPPWVQSGL 2064

  Fly  2765 PN----QLGGGQMPVGGQHGGMGMGMGAPPMAGTVGGVRPSPGAG--GGGGSATGGGLNTQQLAL 2823
            |.    |.|..:.|:      |.:.....||     .:.|..|.|  .|......|.|....|..
Frog  2065 PQSQSMQPGMQRSPI------MSVPQQGQPM-----NMNPQSGIGQVPGASQTKQGSLPQAALQN 2118

  Fly  2824 IMQKIKNNPTNESNQHILAILKQNPQIMAAIIKQR---QQSQNNAAAGGGAPG-PGGA-LQQQQA 2883
            :::.:::..:....|.:|.||..|||::||.||||   ..|..||.|.|..|| |..| |||...
Frog  2119 LLRTLRSPSSPMQQQQVLNILHSNPQLLAAFIKQRAAKYASSQNAQAAGNVPGMPAQAGLQQVVH 2183

  Fly  2884 GNG-----------------PQNPQQQQQQQQQQQVMQQQQMQHM------MNQQQGGGGP---- 2921
            .|.                 ||.||..|||..||..:.||.|..:      :|...||..|
Frog  2184 SNPGMQGMNPMQAIGPRPGMPQQPQAPQQQPPQQSPLPQQAMAGINPQGQPLNINPGGISPQFRE 2248

  Fly  2922 ----------QQMNPNQQQQQQQVNLMQQQQQGGPGGPGSGLPTRMPNMPNALGMLQSLPPNMSP 2976
                      ||....|||||||....||||||  .||
Frog  2249 IILRRQQIMQQQQQQQQQQQQQQQQQQQQQQQG--AGP--------------------------- 2284

  Fly  2977 GVSTQGGMVPNQNWNKMRYMQ-MSQYPPPYPQRQRGPHMGGAGPGPGQQQFPGGGGGAGNFNAGG 3040
                  |||   |.|:.:..| ||.|.....|:||..|
Frog  2285 ------GMV---NHNQFQQHQVMSGYTQQQQQQQRFQH--------------------------- 2313

  Fly  3041 AGGAGGVVGVGGVPGGAGTVPGGDQYSMANAAAASNMLQQQQGQVGVGVGVGVKPGPGQQQQQMG 3105
                                                .:|.|||.:|             |..||.
Frog  2314 ------------------------------------HIQMQQGSMG-------------QMSQMP 2329

  Fly  3106 VGMP----PGMQQQQQQQQPLQQQQMMQVAMPNANAQNPSAVVGGPNAQVMGPP--TPHSLQQQL 3164
            .|||    .|:|...|:   |..|||...|.     |||.:    |...::..|  :||...|||
Frog  2330 QGMPGDTSSGLQTFPQR---LLHQQMGSPAQ-----QNPMS----PQQHMLPGPAQSPHLQGQQL 2382

  Fly  3165 MQSARSSPPIRSPQPTPSPR-------SAPSPRAAPSASPRAQPSPHHVMSSHSPAPQ-GPPHDG 3221
            ..|.  |..:|||||.||||       |:||||..|      |||||||      :|| ..||.|
Frog  2383 PPSL--SNQVRSPQPVPSPRPQSQPPHSSPSPRMQP------QPSPHHV------SPQTSSPHPG 2433

  Fly  3222 MHNHGM-------HHQSPLP------------GVPQDVGVG 3243
            :...|.       |:.||.|            |..||:|:|
Frog  2434 LVGPGQGNPMDQGHYGSPDPTAMLSQMAGLHGGGVQDLGLG 2474

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nej	NP_001188575.1	ZnF_TAZ	530..600	CDD:214717	34/69 (49%)
		KIX	946..1024	CDD:280354	57/77 (74%)
		Bromo_cbp_like	1705..1812	CDD:99927	80/113 (71%)
		RING_CBP-p300	1824..1906	CDD:276805	55/81 (68%)
		PHD_CBP_p300	1908..1939	CDD:277032	20/30 (67%)
		HAT_KAT11	1970..2283	CDD:285432	210/314 (67%)
		ZZ_CBP	2339..2379	CDD:239077	26/39 (67%)
		ZnF_TAZ	2404..2476	CDD:214717	62/71 (87%)
ep300	XP_004913855.2	zf-TAZ	347..414	CDD:396624	33/66 (50%)
		KIX	563..643	CDD:366953	57/79 (72%)
		Med15	<650..>877	CDD:312941	70/368 (19%)
		PHA03247	<777..982	CDD:223021	58/367 (16%)
		Bromo_cbp_like	1080..1194	CDD:99927	80/113 (71%)
		RING_CBP-p300	1206..1288	CDD:276805	55/81 (68%)
		PHD_p300	1289..1323	CDD:277116	21/33 (64%)
		HAT_KAT11	1352..1658	CDD:400497	210/314 (67%)
		ZZ_CBP	1714..1754	CDD:239077	26/39 (67%)
		zf-TAZ	1781..1849	CDD:396624	58/67 (87%)
		Med15	1954..>2451	CDD:312941	187/721 (26%)
		Creb_binding	2059..2161	CDD:401102	29/112 (26%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	430	1.000	Domainoid score	I588
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1418	1.000	Inparanoid score	I125
OMA	1	1.010	-	-		QHG47779
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0001062
OrthoInspector	1	1.000	-	-		otm48572
Panther	1	1.100	-	-	LDO	PTHR13808
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2438
SonicParanoid	1	1.000	-	-		X719
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	10	10.100