DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment unc-13 and Unc13a

DIOPT Version :9

Sequence 1:	NP_001245427.1	Gene:	unc-13 / 43841	FlyBaseID:	FBgn0025726	Length:	3186	Species:	Drosophila melanogaster
Sequence 2:	XP_017168363.1	Gene:	Unc13a / 382018	MGIID:	3051532	Length:	1736	Species:	Mus musculus

Alignment Length:	1951	Identity:	976/1951 - (50%)
Similarity:	1242/1951 - (63%)	Gaps:	313/1951 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly  1310 GLKKKTISSKMSGLMQKAKTYKRHSFVLRRGCNMSDSELEMPDFVSSGNDNSSISTREILLNQSI 1374
            |:||    :|..|..:|..||     |..:..|:..:.:.:     .|:..|
Mouse     7 GVKK----AKFDGAQEKFNTY-----VTLKVQNVKSTTIAV-----RGSQPS------------- 44

  Fly  1375 EVEDEQEDFNYK-NRCDSKSVLGGSIEKLNGNL------------------TNNLFPIVGDLKKI 1420
                .::||.:: ||.|    ||.::|..|..|                  :|...|  |:...:
Mouse    45 ----WEQDFMFEINRLD----LGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGP--GEWLTL 99

  Fly  1421 QSPLPLAVLTEIPSYKDEYSNK--SDSIKNSPIEMPKILLETACN-----QELNLAHSDDDVDKN 1478
            .|...:|. :||...||...::  .|:....|:::|:   |.|..     ::||.....|:|..
Mouse   100 DSQAIMAD-SEICGTKDPTFHRILLDAHFELPLDIPE---EEARYWAKKLEQLNAMRDQDEVSH- 159

  Fly  1479 ILANSAKDYVNAPTFSILKTVEDASEPTMTPLHTTTTTNSSLNVTSALWVTQQCLDLPNYPGWGS 1543
             |..|.:|..:.|                            |.|.|:     ||.:. ||.|||.
Mouse   160 -LQYSFQDQQDKP----------------------------LPVPSS-----QCCNW-NYFGWGE 189

  Fly  1544 REDD-----DNRSQHSARTLSSSRRQSTEDSIDTDDEYFYYELRQLEEQEKQRAHNSAI---PSC 1600
            :.||     |:|        .|..|..|.:||...    ||    ...|.....|..::   |..
Mouse   190 QNDDPDSAVDDR--------DSDYRSETSNSIPPP----YY----TTSQPNASVHQYSVRPPPLG 238

  Fly  1601 ERQNDNDVLFSQIGQLLQNDVNGGDGFRHSNGCNDGEDAIIFSPSESVKLRMSEVFKELKSVVSL 1665
            .|::.:|.:.|                                            ::|.....:|
Mouse   239 SRESYSDSMHS--------------------------------------------YEEFSEPRAL 259

  Fly  1666 NPSVNNDATFEGVPIVKPTYEKLETVSDLHSAWQDVNGDLQIAASDIDSNEDLVGNKGRETPTYN 1730
            :|:.::.....|        |..:..|.|...:......||  .|::|...|
Mouse   260 SPTGSSRYASSG--------ELSQGSSQLSEDFDPDEHSLQ--GSELDDERD------------- 301

  Fly  1731 KQRKLRRLKKKTRDRKINISKNATSSSSSCHSENECNTPLGQCTQKSVAEKDTNDISN----KSE 1791
                        ||           |..||||....:....:..|.....:|..|:.:    :.|
Mouse   302 ------------RD-----------SYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEE 343

  Fly  1792 ASSETSGPDTPAELSDVDISETENGLRADDGQNIDNMRGNSGSLLKVNRKYLLQFDV-----DHS 1851
            ...|..|.:   ||.:.|:.|.|.  ..||..:.......:.:..|..::  :.|..     |..
Mouse   344 EELEEEGEE---ELEEEDLEEEEE--VPDDLASYTQQEDTTVAEPKEFKR--ISFPTAAPQKDDK 401

  Fly  1852 LINQPLETSQYNTHMLENITSASIPSQ----NRQIDSKTLMSQSSHADGSQAVGNETAVGLSSSK 1912
            :...|.|..:    :.:.|..|:.|.:    .|..:::...|:.|.....:..|.|....:|.:|
Mouse   402 VSAVPTEAPE----VAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAK 462

  Fly  1913 WKLLKTLKE--RKIEEKNNQDKIKEDEMIKDRDKNGGGPGEVGLRTNGHPGDNPFYSNIDSMPDI 1975
            ...|:...:  .:::|...:..:.:....|      ||||. ||..            |||||||
Mouse   463 ANWLRAFNKVRMQLQEARGEGDMSKSLWFK------GGPGG-GLII------------IDSMPDI 508

  Fly  1976 RPRRKSIPLVSELVLKTMAATKRNAGLTSAVPRATLNDEDLKMHVYKKALQALIYPISSTTPHNF 2040
            | :||.|||||:|.:..:.:  |.||:|||:..:|||:|:||.|||||.|||||||||.||||||
Mouse   509 R-KRKPIPLVSDLAMSLVQS--RKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNF 570

  Fly  2041 LLWTATSPTYCYECEGLLWGIARQGVRCTECGVKCHEKCKDLLNADCLQRAAEKSSKHGAEDKAN 2105
            .:||||:||||||||||||||||||:|||||||||||||:||||||||||||||||||||||:..
Mouse   571 EVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQ 635

  Fly  2106 SIITAMKDRMKQREREKPEIFELIRAVFSVEEKSHAGHMKAVKQSVLDGTSKWSAKIAITVICAQ 2170
            :||..:|||||.|||.||||||||:.:|:|.:.:|...|||||||||||||||||||:|||:|||
Mouse   636 NIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQ 700

  Fly  2171 GLIAKDKSGTSDPYVTVQVSKVKKRTRTMPQELNPVWNEKFHFECHNSSDRIKVRVWDEDNDLKS 2235
            ||.||||:|:|||||||||.|.||||:|:...|||||.|.||||||||||||||||||||:|:||
Mouse   701 GLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKS 765

  Fly  2236 KLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYH 2300
            :::|:..|||||||||||||||||||||||||||:||||||||||||||||||||||||||||||
Mouse   766 RVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYH 830

  Fly  2301 VQYTCLHENLFHYLCE-ENTGMVKLPTQKGDDAWKLYFDEIPEEIVDEFSMRYGIENIYQAMTHF 2364
            ||||||||||||::.: :|.|:||:|..|||||||:|:||..:||||||:||||:|:||||||||
Mouse   831 VQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHF 895

  Fly  2365 HCLSAKYLCPGVPAVMSTLLANINAYYAHTTASSAVSASDRFAASNFGKEKFVKLLDQLHNSLRI 2429
            .|||:||:|||||||||||||||||||||||||:.|||||||||||||||:||||||||||||||
Mouse   896 ACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRI 960

  Fly  2430 DLSMYRNNFPASSPEKLMDLKSTVDLLTSITFFRMKVQELSSPPRASTVVKDCVKACLRSTYQFL 2494
            |||||||||||||||:|.||||||||||||||||||||||.||||||.||||||||||.|||:::
Mouse   961 DLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYI 1025

  Fly  2495 FENCYELYNREFQVDPNEAKRA---PDDHEPKLDSVDFWHKLIALIVSVIDEDKNSYGTVLNQFP 2556
            |.||:|||.||:|.||  ||:.   |::..|.:.::|||.|||.||||:|:||||||...|||||
Mouse  1026 FNNCHELYGREYQTDP--AKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFP 1088

  Fly  2557 QELNIGQLSASSMWHLFAVDMKYALEEHEQHRLCKSSAYMNLHFRVKWLYSNYVKEVPPYKGAVP 2621
            ||||:|::||..||.|||.|||||:|||::||||||:.||||||:|||||:.||.|:|.:|..||
Mouse  1089 QELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVP 1153

  Fly  2622 DYPAWFEPFVMQWLNENDDVSLEYLHGAFKRDKKDGFQKSSEHALFSNSVVDVFTQLTQCFDVVS 2686
            :|||||||||:|||:||::||.::||||.:||||||||::|||||||.||||||:||.|.|:::.
Mouse  1154 EYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIK 1218

  Fly  2687 KLECPDPEIWKRYMRRFAKTIVKVLIAYADIVKLEFPEH--MKDERIACILMNNIQQLRVQLEKM 2749
            |||||||:|...|||||||||..||:.|||||..:|..:  .:.|::.||||||.||||||||||
Mouse  1219 KLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKM 1283

  Fly  2750 FESMGGDKLEEDAANILKELQQNLNSALDDLASQFAISLEPRITQSVRELGDMLLSIKGGSGTLA 2814
            ||:|||.:|:.:|:..|||||..||:.||:|:..||.|.:|.|.:.||::||:|..:||
Mouse  1284 FEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKG------ 1342

  Fly  2815 AGNLAAQR-NAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLLKELWKIVMRILEKTIVLP 2878
            .||:.|.. ::||.:||.||:|:|||||.:|||:|:.||||||||:||||||:||..:|||||||
Mouse  1343 TGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLP 1407

  Fly  2879 PMTDKTMMFKHLTDNAKNLASNAKIEDMGRL-FKSHMAGKQDVKSALSGVMDISK-------EVE 2935
            |:||:||:...|..:.|.|       :.||: ..||..|.|.:.:|...:..:||       |..
Mouse  1408 PLTDQTMIGTLLRKHGKGL-------EKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEA 1465

  Fly  2936 KNLSPKQCAVLDVALDTIKQYFHAGGNGLKKTFLEKSPELQSLRYALSLYTQMTDTLIKTFI--- 2997
            |:|:||||||:::|||||||||||||.|||||||||||:|||||||||||||.||.|||||:
Mouse  1466 KSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQ 1530

  Fly  2998 SSQVH---------EVDL-----ENSEESVGEISVQIDLFSHPGTGEHKVNVKVVAANDLKWQIP 3048
            |:|||         ..|:     ...|:.|||:||.::||:||||||.||.|||||||||||| .
Mouse  1531 SAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQ-T 1594

  Fly  3049 SGMFRPFVDINLIGPHLQEKKRKFATKSKSNNWSPKYNESFSFTIGNEEQLDFFELHICVKDYCF 3113
            ||:||||:::|::||.|.:||||||||||:|:|:|||||||.|::..:...:.:||.:|||||||
Mouse  1595 SGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCF 1659

  Fly  3114 ARDDRLVGVAVIPLKDISEKGSVACWLPLMRRIEMDETGWTILRILSQRNNDEVAKEFVKLKSEI 3178
            ||:||.||:||:.|::::::||.||||||.|||.||:||.|:|||||||:|||||||||||||:.
Mouse  1660 AREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDT 1724

  Fly  3179 R 3179
            |
Mouse  1725 R 1725

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
unc-13	NP_001245427.1	C1	2038..2087	CDD:237996	43/48 (90%)
		C2B_Munc13	2160..2286	CDD:175993	102/125 (82%)
		DUF1041	2477..2584	CDD:283860	68/109 (62%)
		Membr_traf_MHD	2832..2992	CDD:287505	100/167 (60%)
		C2C_Munc13	3032..3152	CDD:176041	75/119 (63%)
Unc13a	XP_017168363.1	C2A_Munc13	1..127	CDD:176040	32/157 (20%)
		C1	568..617	CDD:237996	43/48 (90%)
		C2B_Munc13	690..816	CDD:175993	102/125 (82%)
		DUF1041	1008..1114	CDD:368829	67/107 (63%)
		Membr_traf_MHD	1360..1522	CDD:371123	100/168 (60%)
		C2C_Munc13	1579..1698	CDD:176041	75/119 (63%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	369	1.000	Domainoid score	I910
eggNOG	1	0.900	-	-		E1_KOG1011
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H11279
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0001626
OrthoInspector	1	1.000	-	-		otm43624
orthoMCL	1	0.900	-	-		OOG6_103034
Panther	1	1.100	-	-	O	PTHR10480
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R10279
SonicParanoid	1	1.000	-	-		X1191
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	12	11.800