Protein Alignment eIF4G1 and EIF4G1

DIOPT Version :10

• Sequence 1: NP_001096852.1
• Gene: eIF4G1 / 43839
• FlyBaseID: FBgn0023213
• Length: 1919
• Species: Drosophila melanogaster
• Sequence 2: NP_001181875.2
• Gene: EIF4G1 / 1981
• HGNCID: 3296
• Length: 1606
• Species: Homo sapiens
• Alignment Length: 1827
• Identity: 483/1827 - (26%)
• Similarity: 745/1827 - (40%)
• Gaps: 442/1827 - (24%)

  Fly   274 PASTAEYV-STGNNNSGNTRSNPQSGGIFRG----------PPSTPNAPRGASGGATR---HVHV 324
            |..||..| ||......||.|.|:.|| ||.          |||:. |.|..|....|   ..||
Human    25 PGQTAPVVFSTPQATQMNTPSQPRQGG-FRSLQHFYPSRAQPPSSA-ASRVQSAAPARPGPAAHV 87

  Fly   325 QPMYSQPLHQNMVLQQYTQYNPRQQTFPASHLQYAPAPMPYYQYQYVPTLQQQPPHTRSAVTVNT 389
            .|..||.:   |:        |.|.::|||...|           |:      |...||...|.|
Human    88 YPAGSQVM---MI--------PSQISYPASQGAY-----------YI------PGQGRSTYVVPT 124

  Fly   390 NVNVGNNLQPVQSGPNGPLPVPGASSSQIQLLTSTVQPGASTVMGVGGPGSTMGQ--VGVPPMVG 452
                  ...|||  |..|...||||.::.           .|..|...|...:.|  .||.|
Human   125 ------QQYPVQ--PGAPGFYPGASPTEF-----------GTYAGAYYPAQGVQQFPTGVAP--- 167

  Fly   453 VGVMTTSVQPQPVQVQPASRRRHQHRLQIIDPTT-KKNILDDFDKTKSNTDNEFSDQVTSTNTPA 516
                |..:..||.|:.|   :|.:..::|.||.. .|:|.:           |......:.:||.
Human   168 ----TPVLMNQPPQIAP---KRERKTIRIRDPNQGGKDITE-----------EIMSGARTASTPT 214

  Fly   517 TVLSEGPIRIPQQE----SVGLNNLTSTSSQGS--ESRTNAP---YIPIETTPISRTDVGPTPIV 572
            ...:.|.:. ||..    .|.:.......|||:  ..|...|   :.|.|:.|.|     |:|
Human   215 PPQTGGGLE-PQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSS-----PSP-- 271

  Fly   573 SAMTDAPSVEILPTPQRGRSKKIPIVS-PKNVSESIVAPITDETDDAGSTPISRATEE--SMPPN 634
               |.:||    |..:.|....:.::| |.:...:|...:.:      ||||||.|.|  .:.|.
Human   272 ---TPSPS----PVLEPGSEPNLAVLSIPGDTMTTIQMSVEE------STPISRETGEPYRLSPE 323

  Fly   635 QT---HPNLLISDSSKHKQAVSNSEISK---DAPTGLK----EMH-----------VAELESS-- 676
            .|   .|.|.:..:.  .:.|..||.|.   .|||.|.    |:|           ..|:|||
Human   324 PTPLAEPILEVEVTL--SKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPE 386

  Fly   677 --------------VASENHPAGILDVNVNKSSQSLDFS----------------ADPEIDSIGA 711
                          :|....|..:|  |...|..::|.|                |.|.|.|  |
Human   387 LAPPPACPSESPVPIAPTAQPEELL--NGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPS--A 447

  Fly   712 ISPTSPNAVSPILHEVLDNTELSKKLENSTTERFKDSQSVEKPTHQELSLRNATDETEISAMALQ 776
            ...|:|:|.||...|     |:.::.|....|..:..::..:...:||.   ..:.|.|.|...|
Human   448 TPATAPSATSPAQEE-----EMEEEEEEEEGEAGEAGEAESEKGGEELL---PPESTPIPANLSQ 504

  Fly   777 DVNSLDNNQIEKTYPSK----PKLNV-----DVSEDISSRESAIKSTSTK-NTGVDVGLQSDS-- 829
            ::.:....|:..:.|.:    .:||.     |:.:.......|:.....: ..|.:.|.:|:.
Human   505 NLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSG 569

  Fly   830 ---KPETTLNDKQDSTDLKVKVSAKI---SSIINYNEGQWSPNNPSGKKQYDREQLLQLREVKAS 888
               :||.. ::..||.:.|:..:..|   .....|...||.|.|...||:||||.||..:.:.||
Human   570 VPPRPEEA-DETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFAS 633

  Fly   889 RIQPE-VKNVS--ILPQPNLMPSFIRNNNNNKRVQSMVGI-IGNRSNESAGNYIGKQMSMSGVQS 949
            ..:|| :.::|  :|.:.|..|  :|..:..:    :.|| .|.....|..|.....:|..|...
Human   634 MQKPEGLPHISDVVLDKANKTP--LRPLDPTR----LQGINCGPDFTPSFANLGRTTLSTRGPPR 692

  Fly   950 G-------------GGRSSMKG------MIHVNLSLNQDVKLSENENAWRP----RVLNKSDGDS 991
            |             |.|.|.:|      .|...:.:.:|:||::.|.||:|    ...:|..|:.
Human   693 GGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEE 757

  Fly   992 DAKSALEKDELVRRVRGILNKLTPERFDTLVEEIIKLKIDTPDKVDEVIVLVFEKAIDEPNFSVS 1056
            ||..:..:| |.||||.|||||||:.|..|::::.:|.|||.:::..||.|:|||||.||||||:
Human   758 DADGSKTQD-LFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVA 821

  Fly  1057 YARLCQRLAAEVKVIDERMESETKSNSAHFRNALLDKTEQEFTQNVSQSTAKEKKLQPIVDKIKK 1121
            ||.:|:.|.| :||    ..:|..:.:.:||..||::.::||.::.......|||.:    ::.:
Human   822 YANMCRCLMA-LKV----PTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQK----EMDE 877

  Fly  1122 CTDANEKAELEAFLEEEERKIRRRSGGTVRFIGELFKISMLTGKIIYSCIDTLLNPHSEDMLECL 1186
            ...|.|:..|:..|||.....||||.|.::|||||||:.|||..|::.|:..||..|.|:.||||
Human   878 AATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECL 942

  Fly  1187 CKLLTTVGAKFEKTPVNSKDPSRCYSLEKSITRMQAIASKTDK--DGARVSSRVRFMLQDVIDLR 1249
            |:||||:|          ||    ...||:..||....::.:|  ...:.|||:|||||||:|||
Human   943 CRLLTTIG----------KD----LDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLR 993

  Fly  1250 KNKWQTSR-NEAPKTMGQIEKEA---------KNEQLSAQYFGTLSSTTPGGSQGGSGKRDDRGN 1304
            .:.|...| ::.|||:.||.|||         |.:||.|:               ||.||  ||.
Human   994 GSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAK---------------GSDKR--RGG 1041

  Fly  1305 SRYGESRSSSAYGGSHSQRG----DNGNLRHQQQNNVGGNVSGGAGHSNGNNDENTWH-VQTSKG 1364
            .           .|....||    |:|.                            |: |..|||
Human  1042 P-----------PGPPISRGLPLVDDGG----------------------------WNTVPISKG 1067

  Fly  1365 SRSLAVDSNKLEGLSKLS--DQNLETKKMGGLTQFIW----------ISSDTTRLSSAPTPTPSN 1417
            ||  .:|:::|..::|..  |.|.:....||  :..|          ..||....::.|..:..|
Human  1068 SR--PIDTSRLTKITKPGSIDSNNQLFAPGG--RLSWGKGSSGGSGAKPSDAASEAARPATSTLN 1128

  Fly  1418 PFAVLSSLIDKNSNERDRDRSGPRNKGSYNKGSMERDRYDRGMHSRTGSSQGSR----------- 1471
            .|:.|...:...|.  |..|...|:..|..:|....||.||...|..|..:|.|
Human  1129 RFSALQQAVPTEST--DNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKR 1191

  Fly  1472 -------ENSSSRGGQQGRTLLSSSVQKSTSHSKYTQQAPPTRHTVKAQSSVGSSNVNTGPLYRG 1529
                   |.|..|..|      ...::|:.|   .|:.....|..||.::::
Human  1192 SFSKEVEERSRERPSQ------PEGLRKAAS---LTEDRDRGRDAVKREAAL------------- 1234

  Fly  1530 SEQQTSATFSQTTRSVAPVAVFIEASETDLKLIKSVVSEIVDLSAASKEVTPGAVSCIKRVPE-K 1593
                         ..|:|:...:...|.: |..|:::.|.:.|:...:     ||.|::.:.. .
Human  1235 -------------PPVSPLKAALSEEELE-KKSKAIIEEYLHLNDMKE-----AVQCVQELASPS 1280

  Fly  1594 LRCSFIYYILTDYLHLANVGKQYRRYLSIAVSQLIQQNYISADHLRLA--YN---EFTVYANDLI 1653
            |...|:.:.:...|..:.:.:::       :.||:.| .:.|.||..|  |.   |....|.|:.
Human  1281 LLFIFVRHGVESTLERSAIAREH-------MGQLLHQ-LLCAGHLSTAQYYQGLYEILELAEDME 1337

  Fly  1654 VDIPELWLYILQFAGPLIVKKILTISDLWNNNLKENSPSNVAKKFLKTYLIYCTQEVGPNFARNM 1718
            :|||.:|||:.:...|::.:..:.:.:|:....|...|...|...|...|....:.:||.....:
Human  1338 IDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTL 1402

  Fly  1719 WIKFNLKWSDFMPE-SEVADFIKFNRLEY-VENESKSPVIDHRETPEKHVKNVIDHIEHLLKEGT 1781
            |.:..|.|.:|:|| .::..|:...::|| :..||::|  ..|..|.:.:..   .:|.|||||:
Human  1403 WREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAP--GQRALPSEELNR---QLEKLLKEGS 1462

  Fly  1782 TADCIIDYSNGNI---MVVDKLFIRGLTETLSNFSIHYKDNSYKLESETFQKFCIPVLQRYIDSN 1843
            :...:.|:...|:   .:|....:|.|...:...:|.: :...:::.... |....:||:|: .:
Human  1463 SNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIF-ETPLRVDVAVL-KARAKLLQKYL-CD 1524

  Fly  1844 EDHQLECLYTLQLLVHGLEHPRGLLSELIGELYDAFVIQKESLCKWRDSKD--QSAGKGVAVKSL 1906
            |..:|:.||.||.||..||.|..||......|||..|:::::...|..|||  :..|||||:||:
Human  1525 EQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSV 1589

  Fly  1907 NPFFNSL 1913
            ..||..|
Human  1590 TAFFKWL 1596

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
eIF4G1	NP_001096852.1	Atrophin-1	<304..>449	CDD:460830	40/149 (27%)
		YppG	322..>379	CDD:372950	13/56 (23%)
		MIF4G	1004..1252	CDD:397130	105/249 (42%)
		MA3	1562..1677	CDD:397128	27/120 (23%)
		W2_eIF4G1_like	1764..1895	CDD:211397	37/135 (27%)
EIF4G1	NP_001181875.2	PHA03247	<21..583	CDD:223021	156/663 (24%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	173..229		16/70 (23%)
		PABPC1-binding	179..207		8/41 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	246..326		25/99 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	339..607		57/280 (20%)
		EIF4E-binding	614..625		6/10 (60%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	674..720		9/45 (20%)
		eIF3/EIF4A-binding	689..1092		162/484 (33%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	738..764		8/25 (32%)
		MIF4G	768..993	CDD:397130	103/247 (42%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1031..1234		57/273 (21%)
		MA3	1249..1361	CDD:397128	28/125 (22%)
		W2_eIF4G1_like	1445..1577	CDD:211397	38/137 (28%)
		EIF4A-binding	1457..1606		46/143 (32%)
		Necessary but not sufficient for MKNK1-binding	1592..1606		3/5 (60%)