DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment bt and TRIO

DIOPT Version :9

Sequence 1:	NP_001162825.1	Gene:	bt / 43814	FlyBaseID:	FBgn0005666	Length:	8933	Species:	Drosophila melanogaster
Sequence 2:	NP_009049.2	Gene:	TRIO / 7204	HGNCID:	12303	Length:	3097	Species:	Homo sapiens

Alignment Length:	2079	Identity:	404/2079 - (19%)
Similarity:	650/2079 - (31%)	Gaps:	806/2079 - (38%)

- Green bases have known domain annotations that are detailed below.

  Fly  6479 W--RPPRTDGKSRIKGYYVEMRPKNGKDW-KTVNDIPINSTVYTVPSLKEGEEYSFRVVAENEVG 6540
            |  |.|.:|.|..:|...:|    |.:|| |.:.::....|::...:|||.........|..:.|
Human  1558 WVGRTPTSDNKIVLKASSIE----NKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKG 1618

  Fly  6541 RSDPSK-------PSQPITIEEQPNKPCMELGKVRDIVCRAGDDFSIHVPYLAFPKPNAFWYSND 6598
            |.|...       .|||.||               .|..|                      ::.
Human  1619 RRDGEDLDSQGDGSSQPDTI---------------SIASR----------------------TSQ 1646

  Fly  6599 NMLDDNNRVHKHLTDDAASVVVKNSKRADSGQYRLQLKNTSGFDTATINVRVLDRP--SPPTRL- 6660
            |.||.:    |.......:||:.:....:|.:..::...|         |.||:||  .|...|
Human  1647 NTLDSD----KLSGGCELTVVIHDFTACNSNELTIRRGQT---------VEVLERPHDKPDWCLV 1698

  Fly  6661 RADEFSGDSLTLYWNPPNDDGGSAIQNYIIEKKEARSSTWSKVSSFCTVPFVRIRNLVLNKEYDF 6725
            |..:.|          |..:|.....:..|  ..:|||             :.:..:..:|  |.
Human  1699 RTTDRS----------PAAEGLVPCGSLCI--AHSRSS-------------MEMEGIFNHK--DS 1736

  Fly  6726 RVIAENKYGQSDPANTSEPILARHPFDIPNTPGIPHGIDSTEDSITIAWTKPKHDGG-------S 6783
            ..::.|  ..|.||:.:.  |..|.....::||                  ||..|.       |
Human  1737 LSVSSN--DASPPASVAS--LQPHMIGAQSSPG------------------PKRPGNTLRKWLTS 1779

  Fly  6784 PI--------TGYIIEKRLLSDDKWTKAVHALC-------PDLSCKIPNLIENAEYEFRVAAVNA 6833
            |:        .|::  |:|....|.::.|....       .|.|...|. .|..|...|...: :
Human  1780 PVRRLSSGKADGHV--KKLAHKHKKSREVRKSADAGSQKDSDDSAATPQ-DETVEERGRNEGL-S 1840

  Fly  6834 AGQSAYSGSSDLIFCRRPPHAPKITSDLSIRDMTVIAGDEFRITVPYHASPRPTASWSLNGLEVI 6898
            :|..:.|.||.:..|...                  .|:|....||.   |.|.|        :.
Human  1841 SGTLSKSSSSGMQSCGEE------------------EGEEGADAVPL---PPPMA--------IQ 1876

  Fly  6899 PGERIKFDSNDYASMYYNKSAKRDETGSYTITLTNNKGSDTASC-------------HVTVVDRP 6950
            ....::.||.|      :|::.|        .|.....|:|.|.             .:.:.|||
Human  1877 QHSLLQPDSQD------DKASSR--------LLVRPTSSETPSAAELVSAIEELVKSKMALEDRP 1927

  Fly  6951 ---LPPQGPLNAYDITP-DTCTLAWKTPLDDGGSPITNYVVEKLDNSGSWVKISSFVRNTHYDVM 7011
               |..||..::....| |...|:..:|:|:         :|:        :.||.::..||.:.
Human  1928 SSLLVDQGDSSSPSFNPSDNSLLSSSSPIDE---------MEE--------RKSSSLKRRHYVLQ 1975

  Fly  7012 GLEPHYKYNFRVRAENQYGLSDPLDIIEPIVA-KHQFTVPDE-PGQPKVIDWDSGNVTLI--WTR 7072
            .|         |..|..| :.|...::|..:| ..:..|||: .|:.|::   .||:..|  |.|
Human  1976 EL---------VETERDY-VRDLGYVVEGYMALMKEDGVPDDMKGKDKIV---FGNIHQIYDWHR 2027

  Fly  7073 PLSDG------------GS-------RIQGYQIEYRDILNDSSWNAYDYI---IKDTKYQLYNLI 7115
            ....|            ||       |:..| |.|......|.....:||   .:|.|.:|.:.:
Human  2028 DFFLGELEKCLEDPEKLGSLFVKHERRLHMY-IAYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRL 2091

  Fly  7116 NGSEYEFRIKAKNAAGLSKPSSPSLRFKLKGKFTVPSPPGAPQVTRVGKNYVDLKWEKPLRDGGS 7180
            ..::.           |.||....::::|                 :.|::  ||:.|...
Human  2092 QLTDL-----------LIKPVQRIMKYQL-----------------LLKDF--LKYSKKAS---- 2122

  Fly  7181 RITGYIIERRDIGGAVWV------KCNDYNVLDTEYTVMNLIEMGDYEFRVFAVNSAGRSEPSLC 7239
                  ::..::..||.|      :|||         :||:..:..::.::.|.......:..|.
Human  2123 ------LDTSELERAVEVMCIVPRRCND---------MMNVGRLQGFDGKIVAQGKLLLQDTFLV 2172

  Fly  7240 T------MP-------------IKVCEVLGGKK----PDWITRLQDKVAPFGKDYTLQ---C--- 7275
            |      :|             :...|.|..||    |.::.:...||:....:..::   |
Human  2173 TDQDAGLLPRCRERRIFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFA 2237

  Fly  7276 -------------AASGKPSPTARWLRNGKEIQMN----------------------GGRMTCDS 7305
                         ..|..||....|:....:|..|                      ||..:..|
Human  2238 LTSRTGDVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGGSGGS 2302

  Fly  7306 KDGVFRLHISNVQTGDDGDYTCEAMNSLGFVNTSGYLKIGSPPIINRC-PSELKLPEGDNSKIKI 7369
            ..|           |..|.   ....|.|..::.|....|..|..:|. ||.:..|
Human  2303 GGG-----------GGSGG---GGAPSGGSGHSGGPSSCGGAPSTSRSRPSRIPQP--------- 2344

  Fly  7370 FYSGDQPLTVILKKNNEVICDSNDDTHVKVNIFDDYVAIYIRN---IVKSDGGPYQIEFTNESGS 7431
                                                    :|:   ::.|.....|.|....||:
Human  2345 ----------------------------------------VRHHPPVLVSSAASSQAEADKMSGT 2369

  Fly  7432 ATGEFYVHITGMPSAP-------TGPMGISYINKNSCMLNWRPPSYD--GGLKVSHYVIERKDVS 7487
            :|..        ||.|       .||...|.          |||..|  |..:.:..:.:.|.:.
Human  2370 STPG--------PSLPPPGAAPEAGPSAPSR----------RPPGADAEGSEREAEPIPKMKVLE 2416

  Fly  7488 SPHWITVSSTCKDTAFNVQGLIENQEYIFRVMAVNENGMGP------------------------ 7528
            ||                           |..|.|.:|..|
Human  2417 SP---------------------------RKGAANASGSSPDAPAKDARASLGTLPLGKPRAGAA 2454

  Fly  7529 -----PLEGLNPIRAKDPIDPPSP-----------PGSPQIT----EIGGDFVHLEWEKP----- 7568
                 ||....|...|:|..|.||           |.||...    ...||...|:.:.|
Human  2455 SPLNSPLSSAVPSLGKEPFPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQTPRHAAP 2519

  Fly  7569 --------------------------ESDGGAHIQGYWIDK-----REVGSNTWQRVNATICAAN 7602
                                      ||...::|....:..     :|...|.:|.....|.|:|
Human  2520 GKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASN 2584

  Fly  7603 QINCINLIEGRQYEFRIFAQNVAGLSTESSASQAVKIIDPQAAS-PPLIVKPLRDANCIQNHNAQ 7666
            |.|.          |.:|         .::..|.     |.|.. .|..|.....|..::|.
Human  2585 QQNM----------FLVF---------RAATDQC-----PAAEGWIPGFVLGHTSAVIVENP--- 2622

  Fly  7667 FTCTINGVPKPTISWY-------KGAREISNGARYHMYSEG--DNHFLNINDVFGEDADEYVCRA 7722
                 :|..|.:.||:       |..::..:|.|     ||  :|
Human  2623 -----DGTLKKSTSWHTALRLRKKSEKKDKDGKR-----EGKLEN-------------------- 2657

  Fly  7723 VNKAGAKSTRATLAIMTAPKL-------NVPPRF---RDTAYFDKGENVVIKIPFTGFPKPRIHW 7777
                |.:.:|..|:...:.||       :|||.|   ......:.||.||::....|.||..|.|
Human  2658 ----GYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITW 2718

  Fly  7778 VRDGEN-IESGGHYTVEVKE-RHAVLIIRDGSHLDSGPYRITAENELGSDTAIIQVQISDRPDPP 7840
            .....| :.:.|||::...: ..|.|.|...:..|.|.|...|.|::||.::...:::.
Human  2719 KGPEHNTLNNDGHYSISYSDLGEATLKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVL------ 2777

  Fly  7841 RFPLIESIGTESLSLSWKAPVWDGCSDITNYYVERREHPLSSWIRVGNTRFTSMAVSGLTPGKEY 7905
                  ..|.:.:.::||
Human  2778 ------GPGMDGIMVTWK----------------------------------------------- 2789

  Fly  7906 DFRIFADNVYGRSDASDTSTLIKTKESVKKKPIERKWEIDANGRKLRGKADGPVKDYDSYVFDIY 7970
                  ||                                                :||:
Human  2790 ------DN------------------------------------------------FDSF----- 2795

  Fly  7971 SKFVPQPVEISQQSVYDRYDILEEIGTGAFGVVHRCRERSTGNIFAAKFIPVSHSVEKDLIRREI 8035
                              |..:.|:|.|.|.||.:|.::.|....|.||:. ...:::|.:..|:
Human  2796 ------------------YSEVAELGRGRFSVVKKCDQKGTKRAVATKFVN-KKLMKRDQVTHEL 2841

  Fly  8036 DIMNQLHHQKLINLHDAFEDDDEMILILEFLSGGELFERITAEGYVMTEAEVINYMRQICEGIRH 8100
            .|:..|.|..|:.|.|.||.....||:||....|.|.:.:...| .:||.::..::.::.|.:|:
Human  2842 GILQSLQHPLLVGLLDTFETPTSYILVLEMADQGRLLDCVVRWG-SLTEGKIRAHLGEVLEAVRY 2905

  Fly  8101 MHEQNIIHLDIKPENIMC-QTRSSTNVKLIDFGLATRLDPNEVVKITTGTAEFAAPEIVNREPVG 8164
            :|...|.|||:|||||:. ::.:...:||.|||.|.:|:....:....|..|||||||:...||.
Human  2906 LHNCRIAHLDLKPENILVDESLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVS 2970

  Fly  8165 FYTDMWATGVLSYVLLSGLSPFAGDNDVQTLKNVKACDWDFDVESFKYISEEAKDFIRKLLVRNK 8229
            ..:|.|:.|||:||||||:|||..|:..:|..|:...|:.|..:.||.:|::||:|:..||..:.
Human  2971 LTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDP 3035

  Fly  8230 EKRMTAHECLLHPWLTGDHSAMKQEINRDRYLAYREKLRRKYEDFERFLLPIGRLSEYSSLRKL 8293
            .||.:|...|...||...:......::..|..::.|  |||:::..|   ||..:..:...|.|
Human  3036 AKRPSAALALQEQWLQAGNGRSTGVLDTSRLTSFIE--RRKHQNDVR---PIRSIKNFLQSRLL 3094

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
bt	NP_001162825.1	I-set	9..101	CDD:254352
		Ig	26..100	CDD:143165
		I-set	119..210	CDD:254352
		Ig	136..209	CDD:143165
		I-set	228..320	CDD:254352
		IGc2	242..312	CDD:197706
		I-set	335..425	CDD:254352
		Ig	350..423	CDD:143165
		Ig	436..530	CDD:299845
		I-set	438..532	CDD:254352
		I-set	545..636	CDD:254352
		Ig	560..633	CDD:143165
		Ig	657..731	CDD:143165
		I-set	746..835	CDD:254352
		Ig	1496..1586	CDD:299845
		IG_like	1503..1588	CDD:214653
		I-set	1639..1724	CDD:254352
		IG	1750..1821	CDD:214652
		IGc2	1750..1806	CDD:197706
		I-set	1826..1909	CDD:254352
		Ig	1831..1897	CDD:299845
		I-set	1914..1998	CDD:254352
		Ig	2020..2095	CDD:299845
		FN3	2100..2194	CDD:238020
		FN3	2203..2292	CDD:238020
		I-set	2311..2395	CDD:254352
		Ig	2321..2395	CDD:299845
		FN3	2399..2493	CDD:238020
		FN3	2501..2590	CDD:238020
		I-set	2602..2693	CDD:254352
		Ig	2619..2693	CDD:299845
		FN3	2697..2793	CDD:238020
		FN3	2801..2893	CDD:238020
		I-set	2902..2995	CDD:254352
		Ig	2919..2993	CDD:299845
		FN3	2999..3088	CDD:238020
		FN3	3102..3191	CDD:238020
		I-set	3208..3292	CDD:254352
		Ig	3219..3292	CDD:299845
		FN3	3296..3390	CDD:238020
		FN3	3400..3489	CDD:238020
		I-set	3494..3590	CDD:254352
		Ig	3516..3590	CDD:299845
		FN3	3594..3682	CDD:238020
		FN3	3694..3781	CDD:238020
		I-set	3795..3885	CDD:254352
		Ig	3813..3885	CDD:299845
		FN3	3889..3980	CDD:238020
		FN3	3990..4082	CDD:238020
		I-set	4090..4181	CDD:254352
		Ig	4108..4181	CDD:299845
		FN3	4185..4271	CDD:238020
		FN3	4285..4383	CDD:238020
		Ig	4404..4480	CDD:299845
		FN3	4484..4571	CDD:238020
		FN3	4584..4677	CDD:238020
		I-set	4685..4775	CDD:254352
		Ig	4703..4775	CDD:299845
		FN3	4779..4863	CDD:238020
		FN3	4879..4970	CDD:238020
		IG	4989..5069	CDD:214652
		Ig	4996..5069	CDD:299845
		FN3	5073..5166	CDD:238020
		FN3	5175..5265	CDD:238020
I-set	5276..5366	CDD:254352
Ig	5293..5366	CDD:299845
FN3	5370..5462	CDD:238020
FN3	5470..5561	CDD:238020
Ig	5559..5654	CDD:299845
I-set	5570..5660	CDD:254352
FN3	5664..5755	CDD:238020
FN3	5764..5853	CDD:238020
I-set	5865..5954	CDD:254352
Ig	5882..5954	CDD:299845
FN3	5958..6049	CDD:238020
FN3	6062..6153	CDD:238020
I-set	6173..6255	CDD:254352
Ig_Titin_like	6182..6255	CDD:143225
FN3	6259..6350	CDD:238020
FN3	6359..6451	CDD:238020
FN3	6470..6552	CDD:238020	22/82 (27%)
I-set	6564..6650	CDD:254352	11/85 (13%)
Ig_Titin_like	6577..6650	CDD:143225	9/72 (13%)
FN3	6654..6745	CDD:238020	17/93 (18%)
FN3	6754..6840	CDD:238020	19/107 (18%)
I-set	6855..6946	CDD:254352	16/103 (16%)
Ig	6874..6946	CDD:299845	14/84 (17%)
FN3	6950..7042	CDD:238020	20/95 (21%)
FN3	7050..7139	CDD:238020	25/113 (22%)
FN3	7151..7237	CDD:238020	13/91 (14%)
I-set	7254..7334	CDD:254352	17/120 (14%)
Ig	7272..7339	CDD:143165	15/107 (14%)
IG_like	7354..7440	CDD:214653	10/88 (11%)
Ig	7366..7440	CDD:299845	7/76 (9%)
FN3	7444..7531	CDD:238020	20/124 (16%)
FN3	7545..7637	CDD:238020	23/142 (16%)
I-set	7648..7737	CDD:254352	17/97 (18%)
Ig	7665..7734	CDD:143165	12/77 (16%)
Ig	7760..7833	CDD:299845	21/74 (28%)
FN3	7837..7927	CDD:238020	5/89 (6%)
STKc_Twitchin_like	7986..8244	CDD:271016	91/258 (35%)
S_TKc	7989..8244	CDD:214567	91/255 (36%)
Ig	8311..8394	CDD:299845
I-set	8312..8401	CDD:254352
I-set	8437..8525	CDD:254352
Ig	8455..8519	CDD:143165
I-set	8632..8721	CDD:254352
IGc2	8646..8711	CDD:197706
I-set	8740..8831	CDD:254352
Ig	8757..8826	CDD:143165
I-set	8839..8922	CDD:254352
Ig	8845..>8909	CDD:299845
TRIO	NP_009049.2	SEC14	69..204	CDD:214706
		SPEC	230..445	CDD:238103
		Spectrin 1	311..418
		SPEC	341..566	CDD:238103
		Spectrin 2	538..644
		SPEC	567..783	CDD:238103
		SPEC	787..1014	CDD:238103
		Spectrin 3	878..984
		SPEC	908..1137	CDD:238103
		Spectrin 4	1109..1216
		SPEC	1143..1243	CDD:197544
		RhoGEF	1295..1465	CDD:238091
		PH1_Kalirin_Trio_like	1472..1594	CDD:270060	11/39 (28%)
		PH	1505..1590	CDD:278594	11/35 (31%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1601..1650		15/85 (18%)
		SH3_Kalirin_1	1659..1718	CDD:212786	15/77 (19%)
		SH3-RhoG_link	1715..1972	CDD:293215	65/359 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1779..1906		33/173 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1927..1965		10/54 (19%)
		RhoGEF	1970..2143	CDD:238091	43/226 (19%)
		PH2_Kalirin_Trio_p63RhoGEF	2148..2286	CDD:270061	18/137 (13%)
		PH	2175..2271	CDD:278594	14/95 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2287..2552		58/372 (16%)
		SH3_Kalirin_2	2555..2613	CDD:212787	15/81 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2639..2660		7/49 (14%)
		I-set	2685..2776	CDD:254352	25/90 (28%)
		Ig	2699..2776	CDD:299845	23/76 (30%)
		STKc_Trio_C	2788..3050	CDD:271015	97/387 (25%)
		S_TKc	2796..3050	CDD:214567	91/255 (36%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.910