DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment bt and Mylk

DIOPT Version :9

Sequence 1:	NP_001162825.1	Gene:	bt / 43814	FlyBaseID:	FBgn0005666	Length:	8933	Species:	Drosophila melanogaster
Sequence 2:	NP_001099344.2	Gene:	Mylk / 288057	RGDID:	1310915	Length:	1961	Species:	Rattus norvegicus

Alignment Length:	2295	Identity:	533/2295 - (23%)
Similarity:	813/2295 - (35%)	Gaps:	675/2295 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly  6543 DPSKPSQ-PITIEEQPNKPCMELGKVRDIVCRAGDDFSIHVPYLAFPKPNAFWYSNDNMLDDNNR 6606
            |||:.|. |:|     ..|...| ..|::..:.|............|:|...|:.|...:.:..|
  Rat    29 DPSRVSSLPLT-----EAPAFIL-PPRNLCVKEGATAKFEGRVRGHPEPQVTWHRNGQTITNGGR 87

  Fly  6607 VHKHLTDDAA----SVVVKNSKRADSGQYRLQLKNTSGFDTATINVRV----LDRPSPPTRLRAD 6663
               .|.|...    |:|::..:..|.|:|..:..|.||....|:.:.|    :.:...|...:|.
  Rat    88 ---FLLDGGVRGTFSLVIRGVREEDKGKYTCEASNGSGARQVTVELTVEGDLMKKHGQPVLSKAS 149

  Fly  6664 EFSGDSLTLYWN--PPN--DDGGSAIQNYIIEKKEARSS-----------TWSKVSSFCTVPFVR 6713
            .|.|::....|.  ||.  ...|.|:   :.|.:..|.|           ||.|.:    ||...
  Rat   150 GFPGETRPSIWGECPPKFATKLGRAV---VKEGQMGRFSCKITGRPPPQVTWLKGN----VPLQP 207

  Fly  6714 IRNLVLNKEYDFRVIAENKYGQSD-------PANTSEPILARHPFDIPN--------------TP 6757
            ...:.::::...:::..::..|.|       ..|.|..........||.              :|
  Rat   208 SARVSMSEKNGMQILEIHEVTQDDMGTYTCMVVNGSGKASMSAELSIPGLDNATRLSRGTKDPSP 272

  Fly  6758 GI----PHGIDSTEDSITIAWTKPKHDGGSPITGYIIEKRLLS-------------DDKWTKAVH 6805
            .|    .:||  ::|..|:|.:|   :..||.....:.:...|             :|...|.:.
  Rat   273 DIRKEVTNGI--SKDPETVAESK---NCSSPQKSNFLAQATNSHLKSLQQPKLKPCEDSPRKVLQ 332

  Fly  6806 -ALCPDLSCKIPNLIENAEYEFRVAAVNAAG-------------------QSAYSGSSDLIFC-- 6848
             ::....|..|.......:.|.|.|.:.|:|                   |....||....|.
  Rat   333 SSVLQKTSSTIVLQASKVQPEVRAAGIGASGPGEERKSLTAPRQATPPTRQCGLGGSVGNKFITG 397

  Fly  6849 ------RRPPHAPKITSDLSIRDMTVIAGDEFRITVPYHASPRPTASWSLNGLEVIPGERIKFDS 6907
                  :|....||..|....:::|  .|...:.|......|:|...|.|.|:.|...|.|....
  Rat   398 NIPIENQRESRFPKFESQPQSQEVT--EGQTVKFTCEVSGIPKPEVGWFLEGIPVRRQEGIVEIY 460

  Fly  6908 NDYASMY--YNKSAKRDETGSYTITLTNNKGSDTASCHVTV-VDRPLPPQGPLNAYDITPDTCTL 6969
            .|..|.|  ..::..|| :|.|:.|.:|..|.  .||..|: ||||
  Rat   461 EDGTSHYLCLPRAGTRD-SGRYSCTASNTLGQ--VSCSWTLRVDRP------------------- 503

  Fly  6970 AWKTPLDDGGSPITNYVVEKLDNSGSWVKISSFVRNTHYDVMGLEPHYKYNFRVRAENQYGLSDP 7034

  Rat   504 ----------------------------------------------------------------- 503

  Fly  7035 LDIIEPIVAKHQFTVPDEPGQPKVIDWDSGNVTLIWTRPLSDGGSRIQGYQIEYRDILNDSSWNA 7099
                                            :|..|.|                         :
  Rat   504 --------------------------------SLTQTAP-------------------------S 511

  Fly  7100 YDYIIKDTKYQLYNLINGSEYEFRIKAKNAAGLSKPSSPSLRFKLKGKFTVPSPPGAPQVTRVGK 7164
            :..::||:.     :|.|.::                  .||..::|     :|  |||||.: .
  Rat   512 FSSVLKDSV-----VIEGQDF------------------VLRCSVRG-----TP--APQVTWL-L 545

  Fly  7165 NYVDLKWEKPLRDGGSRITGYIIERRDIGGAVWVKCNDYNVLDTEYTVMNLIEMGDYEFRVFAVN 7229
            |...:::.:.:.:.|                           :.|..:.:.:......:...|.|
  Rat   546 NGQPIQFARSICEAG---------------------------EAELHIQDALPEDRGTYTCLAEN 583

  Fly  7230 SAGRSEPSLCTMPIKVCEVLG-GKK-------------PDWITRLQDKVAPFGKDYTLQCAASGK 7280
            :.|:..   |:..:.|.|..| |::             |.::..|.|.....|...|:....||.
  Rat   584 ALGQVS---CSATVTVQEKKGEGERAHRLPPAPSKLFAPIFLQGLSDLKVMDGSQVTMTVQVSGN 645

  Fly  7281 PSPTARWLRNGKEIQMNGGRMTCDSKDGVFRLHISNVQTGDDGDYTCEAMNSLGFVNTSGYLKI- 7344
            |.|...||.:|.||| .......:.|.|...|.|..|...|.|.|||||.||.|.|.|...|.:
  Rat   646 PPPEVIWLHDGNEIQ-ESEDFHFEQKGGWHSLCIQEVFPEDTGTYTCEAWNSAGEVRTQAILTVQ 709

  Fly  7345 ----GSPPIINRCPSELKLPEGDNSKIKIFYSGDQPLTVILKKNNEVICDSNDDTHVKVNIFDDY 7405
                |:.|.....|..:....|.:..|....:||...||...::.:.:  |.|..|.::...:|.
  Rat   710 EPHDGTQPWFISKPRSVTASLGQSVLISCAIAGDPFPTVHWLRDGKAL--SRDHGHFELLQNEDV 772

  Fly  7406 VAIYIRNIVKSDGGPYQIEFTNESGSATGEFYVHITGMPSAPTGPMGISYINKNSCM-------- 7462
            ..:.::|:.....|.|:|...|..|..:.:..:.:...||....|.|   ....||.
  Rat   773 FTLVLKNVQPWHAGQYEILLKNRVGECSCQVSLMLHKGPSRVPPPRG---REPASCEGLCGGGVG 834

  Fly  7463 -------------------LNWRPPSYDG-----------------------------------G 7473
                               ..| |...||                                   |
  Rat   835 AHGDADRDGTLRPCWPARGQGW-PEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLG 898

  Fly  7474 LKVSHYVIERKDVSSPHWITVSSTCKDTAFNVQGLIE-------------NQEYIFR-VMAVNEN 7524
            .|||...:...|:..     :.:...|...|:|..::             .|:..|| |:|....
  Rat   899 KKVSTKTVSEDDLKD-----IPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGT 958

  Fly  7525 GMGPPLEGLNPIRAKDPIDPPSPPGSPQIT--EIGGDFVHL----EWEKPESDGGAHIQGYWIDK 7583
            ...|..|.:.|.:|..| |..|..||.:.:  |.||:...:    ..|.|...|.|...|   ..
  Rat   959 PKTPVPEKVPPQKAATP-DFRSVLGSKKKSPAENGGNTAEVLNVKAGESPTPAGNAQPVG---AL 1019

  Fly  7584 REVG----SNTWQRV-------------NATICAANQINCINLIEGRQYEFRIF-AQ-------- 7622
            :.:|    :.|.:.|             ||.  .|..:..:..::..:....|. ||
  Rat  1020 KPIGNAKPAETLKSVGNAKPAETLKPVGNAK--PAETLKPVGNVKPAETLKPIANAQPSGSPKPI 1082

  Fly  7623 -NVAGLSTESSASQAVKIIDPQAA--------------------------------SPPLIVKPL 7654
             |...:.|:.|.:.|.....|:.|                                |.|:..:.|
  Rat  1083 ANAQPIETQKSVANAKSAETPKLAGKEELKEVRNDVNCKKAHVGATGNEKRPESQGSAPVFKEKL 1147

  Fly  7655 RDANCIQNHNAQFTCTINGVPKPTISWYKGAREISNGARYHMYSEGDNHFLNINDVFGEDADEYV 7719
            :|.:..:.......|.:...|..|::|....:.:.......:..||....::|.....||...|.
  Rat  1148 QDVHVAEGEKLLLQCQVTSDPPATVTWTLNGKTLKTTKFIILAQEGSLFSVSIEKALPEDRGLYK 1212

  Fly  7720 CRAVNKAG-----------------------AKSTR--------------ATL----AIMTAPKL 7743
            |.|.|.||                       .||.|              ||:    |..|..|.
  Rat  1213 CVAKNSAGQAECSCQVTVDDAGTSENTKAPEMKSRRPKSSLTPVLGTESDATVKKKPASKTPTKA 1277

  Fly  7744 NVPP---RFRDTAYFDKGENVVIKIPFTGFPKPRIHWVRDGENIESGGHYTVEVKERHAVLIIRD 7805
            .:||   :|.:......||.|.:....||.......|::..:.|:...:..||..|..:.|.|..
  Rat  1278 AMPPQIIQFPEDQKVRAGEPVELFGKVTGTQPITCTWMKFRKQIQESENIRVENSESGSKLTILA 1342

  Fly  7806 GSHLDSGPYRITAENELGSDTAIIQVQISDRPDPPR-FPLIESIGTESLSLSWKAPVWDGCSDIT 7869
            ......|.|.:..||:|.|..|.:.:.:.|:||||. .|....|.:.||:|||....:||.|.:.
  Rat  1343 ARQEHCGCYTLVVENKLSSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQ 1407

  Fly  7870 NYYVERREHPLSSWIRVGNTRFTSMAVSGLTPGKEYDFRIFADNVYGRSDASDTSTLIKTKESVK 7934
            :|.||..:.....|..:...|.||..|..|.|.:||.||:.|.||||.|:.|..|.|    .:|.
  Rat  1408 SYNVEIWDTEDKVWTELATCRSTSFNVQDLLPDREYKFRVRAVNVYGTSEPSQESEL----TAVG 1468

  Fly  7935 KKPIERKWEIDANGRKLRGKADGPVKDYDSYVFDIYSKFVPQPVEISQQSVYDRYDILEEIGTGA 7999
            :||.|.|.|::.:.    .....|..||.:...:            ::|.|.|.|||.|.:|:|.
  Rat  1469 EKPEEPKDEVEVSD----DDEKEPEVDYRTVTVN------------TEQKVSDVYDIEERLGSGK 1517

  Fly  8000 FGVVHRCRERSTGNIFAAKFIPVSHSVEKDLIRREIDIMNQLHHQKLINLHDAFEDDDEMILILE 8064
            ||.|.|..|:.||.|:|.||.....:.||:.||:||.|||.|||.||:...||||:...::::||
  Rat  1518 FGQVFRLVEKRTGKIWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLE 1582

  Fly  8065 FLSGGELFERITAEGYVMTEAEVINYMRQICEGIRHMHEQNIIHLDIKPENIMCQTRSSTNVKLI 8129
            .:|||||||||..|.:.:||.|.|.|||||.||:.::|:|.|:|||:|||||||..::.|.:|||
  Rat  1583 IVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLI 1647

  Fly  8130 DFGLATRLDPNEVVKITTGTAEFAAPEIVNREPVGFYTDMWATGVLSYVLLSGLSPFAGDNDVQT 8194
            |||||.||:....:|:..||.||.|||::|.||:|:.||||:.||:.|:|:||||||.||||.:|
  Rat  1648 DFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNET 1712

  Fly  8195 LKNVKACDWDFDVESFKYISEEAKDFIRKLLVRNKEKRMTAHECLLHPWLTGDHSAMK-QEINRD 8258
            |.||.:..||||.|:|..|||:|||||..||.::.:.|:...:||.||||..|...|: :::::|
  Rat  1713 LANVTSATWDFDDEAFDEISEDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKD 1777

  Fly  8259 RYLAYREKLRRKYEDFERFLLPIGRLSEYSSLRKLLMEKYKIHDAVFDRRQAAPRFVIRPSSQFC 8323
            |...|  ..|||::.....:..|||||..:.:..|...|
  Rat  1778 RMKKY--MARRKWQKTGNAVRAIGRLSSMAMISGLSGRK-------------------------- 1814

  Fly  8324 YEGQSVKFYCRCIAIATPTLTWSHNNIELRQSVKFMKRYVGDDYYFIINRVKLDDRGEYIIRAEN 8388
                        .:..:||..                          ||..||:
  Rat  1815 ------------SSTGSPTSP--------------------------INAEKLE----------- 1830

  Fly  8389 HYGSREEVVFLNVQPLPKEQPRYRTESTPVRRREPLPYTFWQEESETAPSFTFLLRPRVMQARDT 8453
               |.|:|....::.:.:|:|..:                        |.|:..:|...:.....
  Rat  1831 ---SEEDVSQAFLEAVAEEKPHVK------------------------PYFSKTIRDLEVVEGSA 1868

  Fly  8454 CKLLCCLSGKPVPNVRWYKDGREL--SKYEYAMTHSDGVVTMEIIDCKPSDSGKYSCKATNCHGT 8516
            .:..|.:.|.|.|.|.|:||.:.:  |::.......||..::.|.|....|..||:|||.|..|.
  Rat  1869 ARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGE 1933

  Fly  8517 DETDCVVIVEGEWVTPEQAQ 8536
            ......:|||    |.|:.:
  Rat  1934 ATCTAELIVE----TMEEGE 1949

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
bt	NP_001162825.1	I-set	9..101	CDD:254352
		Ig	26..100	CDD:143165
		I-set	119..210	CDD:254352
		Ig	136..209	CDD:143165
		I-set	228..320	CDD:254352
		IGc2	242..312	CDD:197706
		I-set	335..425	CDD:254352
		Ig	350..423	CDD:143165
		Ig	436..530	CDD:299845
		I-set	438..532	CDD:254352
		I-set	545..636	CDD:254352
		Ig	560..633	CDD:143165
		Ig	657..731	CDD:143165
		I-set	746..835	CDD:254352
		Ig	1496..1586	CDD:299845
		IG_like	1503..1588	CDD:214653
		I-set	1639..1724	CDD:254352
		IG	1750..1821	CDD:214652
		IGc2	1750..1806	CDD:197706
		I-set	1826..1909	CDD:254352
		Ig	1831..1897	CDD:299845
		I-set	1914..1998	CDD:254352
		Ig	2020..2095	CDD:299845
		FN3	2100..2194	CDD:238020
		FN3	2203..2292	CDD:238020
		I-set	2311..2395	CDD:254352
		Ig	2321..2395	CDD:299845
		FN3	2399..2493	CDD:238020
		FN3	2501..2590	CDD:238020
		I-set	2602..2693	CDD:254352
		Ig	2619..2693	CDD:299845
		FN3	2697..2793	CDD:238020
		FN3	2801..2893	CDD:238020
		I-set	2902..2995	CDD:254352
		Ig	2919..2993	CDD:299845
		FN3	2999..3088	CDD:238020
		FN3	3102..3191	CDD:238020
		I-set	3208..3292	CDD:254352
		Ig	3219..3292	CDD:299845
		FN3	3296..3390	CDD:238020
		FN3	3400..3489	CDD:238020
		I-set	3494..3590	CDD:254352
		Ig	3516..3590	CDD:299845
		FN3	3594..3682	CDD:238020
		FN3	3694..3781	CDD:238020
		I-set	3795..3885	CDD:254352
		Ig	3813..3885	CDD:299845
		FN3	3889..3980	CDD:238020
		FN3	3990..4082	CDD:238020
		I-set	4090..4181	CDD:254352
		Ig	4108..4181	CDD:299845
		FN3	4185..4271	CDD:238020
		FN3	4285..4383	CDD:238020
		Ig	4404..4480	CDD:299845
		FN3	4484..4571	CDD:238020
		FN3	4584..4677	CDD:238020
		I-set	4685..4775	CDD:254352
		Ig	4703..4775	CDD:299845
		FN3	4779..4863	CDD:238020
		FN3	4879..4970	CDD:238020
		IG	4989..5069	CDD:214652
		Ig	4996..5069	CDD:299845
		FN3	5073..5166	CDD:238020
		FN3	5175..5265	CDD:238020
I-set	5276..5366	CDD:254352
Ig	5293..5366	CDD:299845
FN3	5370..5462	CDD:238020
FN3	5470..5561	CDD:238020
Ig	5559..5654	CDD:299845
I-set	5570..5660	CDD:254352
FN3	5664..5755	CDD:238020
FN3	5764..5853	CDD:238020
I-set	5865..5954	CDD:254352
Ig	5882..5954	CDD:299845
FN3	5958..6049	CDD:238020
FN3	6062..6153	CDD:238020
I-set	6173..6255	CDD:254352
Ig_Titin_like	6182..6255	CDD:143225
FN3	6259..6350	CDD:238020
FN3	6359..6451	CDD:238020
FN3	6470..6552	CDD:238020	5/9 (56%)
I-set	6564..6650	CDD:254352	19/89 (21%)
Ig_Titin_like	6577..6650	CDD:143225	16/76 (21%)
FN3	6654..6745	CDD:238020	21/112 (19%)
FN3	6754..6840	CDD:238020	22/136 (16%)
I-set	6855..6946	CDD:254352	26/92 (28%)
Ig	6874..6946	CDD:299845	21/73 (29%)
FN3	6950..7042	CDD:238020	1/91 (1%)
FN3	7050..7139	CDD:238020	7/88 (8%)
FN3	7151..7237	CDD:238020	12/85 (14%)
I-set	7254..7334	CDD:254352	29/79 (37%)
Ig	7272..7339	CDD:143165	27/66 (41%)
IG_like	7354..7440	CDD:214653	17/85 (20%)
Ig	7366..7440	CDD:299845	15/73 (21%)
FN3	7444..7531	CDD:238020	24/162 (15%)
FN3	7545..7637	CDD:238020	23/124 (19%)
I-set	7648..7737	CDD:254352	23/129 (18%)
Ig	7665..7734	CDD:143165	18/105 (17%)
Ig	7760..7833	CDD:299845	17/72 (24%)
FN3	7837..7927	CDD:238020	36/90 (40%)
STKc_Twitchin_like	7986..8244	CDD:271016	138/257 (54%)
S_TKc	7989..8244	CDD:214567	137/254 (54%)
Ig	8311..8394	CDD:299845	7/82 (9%)
I-set	8312..8401	CDD:254352	9/88 (10%)
I-set	8437..8525	CDD:254352	24/89 (27%)
Ig	8455..8519	CDD:143165	21/65 (32%)
I-set	8632..8721	CDD:254352
IGc2	8646..8711	CDD:197706
I-set	8740..8831	CDD:254352
Ig	8757..8826	CDD:143165
I-set	8839..8922	CDD:254352
Ig	8845..>8909	CDD:299845
Mylk	NP_001099344.2	I-set	42..132	CDD:254352	20/93 (22%)
		Ig	64..129	CDD:143165	16/67 (24%)
		I-set	165..254	CDD:254352	15/95 (16%)
		Ig	183..251	CDD:143165	11/71 (15%)
		23ISL	255..408	CDD:293226	26/157 (17%)
		I-set	410..500	CDD:254352	27/94 (29%)
		Ig	427..497	CDD:143165	21/72 (29%)
		I-set	510..596	CDD:254352	21/171 (12%)
		IGc2	523..586	CDD:197706	15/115 (13%)
		I-set	619..708	CDD:254352	33/89 (37%)
		Ig	636..704	CDD:143165	28/68 (41%)
		I-set	717..805	CDD:254352	18/89 (20%)
		Ig	720..805	CDD:299845	17/86 (20%)
		I-set	1141..1230	CDD:254352	18/88 (20%)
		IGc2	1154..1220	CDD:197706	13/65 (20%)
		Ig	1281..1378	CDD:299845	25/96 (26%)
		I-set	1281..1370	CDD:254352	21/88 (24%)
		FN3	1374..1465	CDD:238020	37/94 (39%)
		STKc_MLCK1	1504..1762	CDD:271093	138/257 (54%)
		Pkinase	1507..1762	CDD:278497	137/254 (54%)
		I-set	1852..1942	CDD:254352	24/89 (27%)
		IGc2	1865..1932	CDD:197706	20/66 (30%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	76	1.000	Domainoid score	I8754
eggNOG	1	0.900	-	-		E1_KOG0613
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.810