DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment bt and Trio

DIOPT Version :9

Sequence 1:	NP_001162825.1	Gene:	bt / 43814	FlyBaseID:	FBgn0005666	Length:	8933	Species:	Drosophila melanogaster
Sequence 2:	NP_001074771.1	Gene:	Trio / 223435	MGIID:	1927230	Length:	3103	Species:	Mus musculus

Alignment Length:	2082	Identity:	401/2082 - (19%)
Similarity:	645/2082 - (30%)	Gaps:	806/2082 - (38%)

- Green bases have known domain annotations that are detailed below.

  Fly  6479 W--RPPRTDGKSRIKGYYVEMRPKNGKDW-KTVNDIPINSTVYTVPSLKEGEEYSFRVVAENEVG 6540
            |  |.|.:|.|..:|...:|    |.:|| |.:.::....||:...:|||.........|..:.|
Mouse  1558 WVGRTPTSDNKIVLKASSIE----NKQDWIKHIREVIQERTVHLRGALKEPIHIPKTAPAARQKG 1618

  Fly  6541 RSDPSK-------PSQPITIEEQPNKPCMELGKVRDIVCRAGDDFSIHVPYLAFPKPNAFWYSND 6598
            |.|...       .|||.||               .|..|                      ::.
Mouse  1619 RRDGEDLDSQGDGSSQPDTI---------------SIASR----------------------TSQ 1646

  Fly  6599 NMLDDNNRVHKHLTDDAASVVVKNSKRADSGQYRLQLKNTSGFDTATINVRVLDRP--SPPTRL- 6660
            |.||.:    |.......:||:.:....:|.:..::...|         |.||:||  .|...|
Mouse  1647 NTLDSD----KLSGGCELTVVIHDFTACNSNELTIRRGQT---------VEVLERPHDKPDWCLV 1698

  Fly  6661 RADEFSGDSLTLYWNPPNDDGGSAIQNYIIEKKEARSSTWSKVSSFCTVPFVRIRNLVLNKEYDF 6725
            |..:.|          |..:|.....:..|  ..:|||             :.:..:..:|  |.
Mouse  1699 RTTDRS----------PAAEGLVPCGSLCI--AHSRSS-------------MEMEGIFNHK--DS 1736

  Fly  6726 RVIAENKYGQSDPANTSEPILARHPFDIPNTPGIPHGIDSTEDSITIAWTKPKHDGG-------S 6783
            ..::.|  ..|.||:.:.  |..|.....::||                  ||..|.       |
Mouse  1737 LSVSSN--DASPPASVAS--LQPHMIGAQSSPG------------------PKRPGNTLRKWLTS 1779

  Fly  6784 PI--------TGYIIEKRLLSDDKWTKAVHALC-------PDLSCKIPNLIENAEYEFRVAAVNA 6833
            |:        .|:  .|:|....|.::.|....       .|.|...|. .|..|...|...: :
Mouse  1780 PVRRLSSGKADGH--AKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQ-DETIEERGRNEGL-S 1840

  Fly  6834 AGQSAYSGSSDLIFCRRPPHAPKITSDLSIRDMTVIAGDEFRITVPYHASPRPTASWSLNGLEVI 6898
            :|..:.|.||.:..|...                  .|:|....||.   |.|.|        :.
Mouse  1841 SGTLSKSSSSGMQSCGEE------------------EGEEGADAVPL---PPPMA--------IQ 1876

  Fly  6899 PGERIKFDSNDYASMYYNKSAKRDETGSYTITLTNNKGSDTASC-------------HVTVVDRP 6950
            ....::.||.|      :|::.|        .|.....|:|.|.             .:.:.|||
Mouse  1877 QHSLLQPDSQD------DKASSR--------LLVRPTSSETPSAAELVSAIEELVKSKMALEDRP 1927

  Fly  6951 ---LPPQGPLNAYDITP-DTCTLAWKTPLDDGGSPITNYVVEKLDNSGSWVKISSFVRNTHYDVM 7011
               |..||..::....| |...|:..:|:|:        :.|:        |.||..|.
Mouse  1928 SSLLVDQGDSSSPSFNPSDNSLLSSSSPIDE--------MEER--------KCSSLKRR------ 1970

  Fly  7012 GLEPHYKYNFRVRAENQYGLSDPLDIIEPIVA-KHQFTVPDE-PGQPKVIDWDSGNVTLI--WTR 7072
                ||.....|..|..| :.|...::|..:| ..:..|||: .|:.|::   .||:..|  |.|
Mouse  1971 ----HYVLQELVETERDY-VRDLGCVVEGYMALMKEDGVPDDMKGKDKIV---FGNIHQIYDWHR 2027

  Fly  7073 PLSDGGSRIQGYQIEYRDILNDSSWNAYDYIIKDTKYQLY-----------NLINGSEY--EFRI 7124
            ....|         |....|.|.......::..:.:..:|           :::  |||  .|..
Mouse  2028 DFFLG---------ELEKCLEDPEKLGSLFVKHERRLHMYIVYCQNKPKSEHIV--SEYIDTFFE 2081

  Fly  7125 KAKNAAG--------LSKPSSPSLRFKLKGKFTVPSPPGAPQVTRVGKNYVDLKWEKPLRDGGSR 7181
            ..|...|        |.||....::::|                 :.|::  ||:.|.    .|.
Mouse  2082 DLKQRLGHRLQLTDLLIKPVQRIMKYQL-----------------LLKDF--LKYSKK----ASL 2123

  Fly  7182 ITGYIIERRDIGGAVWVKCNDYNVLDTEYTVMNLIEMGDYEFRVFAVNSAGRSEPSLCT------ 7240
            .|..:.:..::...|..:|||         :||:..:..::.::.|.......:..|.|
Mouse  2124 DTSELEKAVEVMCIVPKRCND---------MMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGL 2179

  Fly  7241 MP-------------IKVCEVLGGKK----PDWI--------------------------TRLQD 7262
            :|             :...|.|..||    |.::                          :|..|
Mouse  2180 LPRCKERRVFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVESDPCKFALTSRTGD 2244

  Fly  7263 KVAPFGKDYTLQCAASGKPSPTARWLRNGKEIQMN--------------------GGRMTCDSKD 7307
            .|..|       ...|..||....|:....:|..|                    ||........
Mouse  2245 AVETF-------VLHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGSGSGGSS 2302

  Fly  7308 GVFRLHISNVQTGDDGDYTCEAMNSLGFVNTSGYLKIGSPPIINRC-PSELKLP----------- 7360
            |           |..|.....|.:.....:.||.....|.|..:|. ||.:..|
Mouse  2303 G-----------GGGGSGGSGASSGGSSSHGSGPSSCSSGPSSSRSRPSRIPQPVRHHPPMLVSS 2356

  Fly  7361 ------EGD---------------NSKIKIFYSGDQPLTVILKKNNEVICDSNDDTHVKVNIFDD 7404
                  |.|               :|.:.:..|..||..:.|..::|. .:...:...|:.:.:.
Mouse  2357 AASSQAEADKMSGMSAPSPSLPTPSSSLALEASLGQPSRLPLSGDSEG-HERETEPIPKMKVMES 2420

  Fly  7405 YVAIYIRNIVKSDGGPYQIEFTNESGSATGEFYVHITGMPSAPTGPMGISYINK----------- 7458
                 .|....|..|      |::.|: |.:...::..:|...|.|..:|.:|.
Mouse  2421 -----PRKAPGSTSG------TSQDGN-TKDARGNLGSLPLGKTRPGAVSPLNSPLSTTFPSPFG 2473

  Fly  7459 -----NSCMLN-----W---------RPPSY------DGGLKVSH-YVIERKDV------SSPHW 7491
                 .|..|.     |         ||.|:      |...:.:| :....||.      ||...
Mouse  2474 KEAFPPSSPLQKGGSFWSSIPASPASRPSSFTFPGDSDSLQRQTHRHAAPSKDTDRMSTCSSASE 2538

  Fly  7492 ITVSSTCKD-----TAFNVQGLIENQEY--------------IFRVMAVNENGM----------G 7527
            .:|.||..:     ::.|:..::...||              :.:::|.|:..|          .
Mouse  2539 QSVQSTQSNGSEGSSSSNISTMLVTHEYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQC 2603

  Fly  7528 PPLEGLNP---IRAKDPIDPPSPPGSPQITEIGGDFVHL--EWEKPESDG---GAHIQGYWIDKR 7584
            |..||..|   :.....:...:|.|:.:.:......:.|  :.||.:.||   |....|| ...|
Mouse  2604 PAAEGWIPGFVLGHTSAVIMENPDGTLKKSTSWHTALRLRKKSEKKDKDGKRDGKLENGY-RKPR 2667

  Fly  7585 EVGSNTWQRVNATICAANQINCINLIEGRQYEFRIFAQNVAGLSTESSASQAVKIIDPQAA--SP 7647
            |                                        |||.:.|    ||:::|...  .|
Mouse  2668 E----------------------------------------GLSNKVS----VKLLNPNYIYDVP 2688

  Fly  7648 PLIVKPLRDANCIQNHNAQFTCTINGVPKPTISWYKGARE--ISNGARYHM-YSEGDNHFLNIND 7709
            |..|.||.:..|.......|.|.:.|.||.:|:| ||...  ::|...|.: ||:.....|.|..
Mouse  2689 PEFVIPLSEVTCETGETVVFRCRVCGRPKASITW-KGPEHNTLNNDDHYSISYSDIGEATLKIIG 2752

  Fly  7710 VFGEDADEYVCRAVNKAGAKSTRATLAIMTAPKLNVPPRFRDTAYFDKGENVVIKIPFTGFPKPR 7774
            |..||...|.|.|||..|:.|:.|:|.::
Mouse  2753 VSTEDDGIYTCIAVNDMGSASSSASLRVL------------------------------------ 2781

  Fly  7775 IHWVRDGENIESGGHYTVEVKERHAVLIIRDGSHLDSGPYRITAENELGSDTAIIQVQISDRPDP 7839
                                                 ||
Mouse  2782 -------------------------------------GP-------------------------- 2783

  Fly  7840 PRFPLIESIGTESLSLSWKAPVWDGCSDITNYYVERREHPLSSWIRVGNTRFTSMAVSGLTPGKE 7904
                     |::.:.::||
Mouse  2784 ---------GSDGIVVTWK---------------------------------------------- 2793

  Fly  7905 YDFRIFADNVYGRSDASDTSTLIKTKESVKKKPIERKWEIDANGRKLRGKADGPVKDYDSYVFDI 7969
                   ||                                                     ||.
Mouse  2794 -------DN-----------------------------------------------------FDA 2798

  Fly  7970 YSKFVPQPVEISQQSVYDRYDILEEIGTGAFGVVHRCRERSTGNIFAAKFIPVSHSVEKDLIRRE 8034
            :                  |..:.|:|.|.|.||.:|.::.|....|.||:. ...:::|.:..|
Mouse  2799 F------------------YSEVAELGRGRFAVVKKCDQKGTKRAVATKFVN-KKLMKRDQVTHE 2844

  Fly  8035 IDIMNQLHHQKLINLHDAFEDDDEMILILEFLSGGELFERITAEGYVMTEAEVINYMRQICEGIR 8099
            :.|:..|.|..|::|.|.||.....:|:||....|.|.:.:...| .:||.:|..::.::.|.:|
Mouse  2845 LGILQNLQHPLLVSLLDTFETPTSYVLVLEMADQGRLLDCVVRWG-SLTEGKVRAHLGEVLEAVR 2908

  Fly  8100 HMHEQNIIHLDIKPENIMC-QTRSSTNVKLIDFGLATRLDPNEVVKITTGTAEFAAPEIVNREPV 8163
            ::|...|.|||:|||||:. |:.:...:||.|||.|.:|:....:....|..|||||||:...||
Mouse  2909 YLHNCRIAHLDLKPENILVDQSLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPV 2973

  Fly  8164 GFYTDMWATGVLSYVLLSGLSPFAGDNDVQTLKNVKACDWDFDVESFKYISEEAKDFIRKLLVRN 8228
            ....|.|:.|||:||||||:|||..|:..:|..|:...|:.|..:.|:.:|::||:|:..||..:
Mouse  2974 SLTADTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPEDYFQGVSQKAKEFVCFLLQED 3038

  Fly  8229 KEKRMTAHECLLHPWLTGDHSAMKQE--INRDRYLAYREKLRRKYEDFERFLLPIGRLSEYSSLR 8291
            ..||.:|...|...||...:.:.|..  ::..|..::.|  |||:::..|   ||..:..:...|
Mouse  3039 PAKRPSAALALQEQWLQAGNGSGKGTGVLDTSRLTSFIE--RRKHQNDVR---PIRSIKNFLQSR 3098

  Fly  8292 KL 8293
            .|
Mouse  3099 LL 3100

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
bt	NP_001162825.1	I-set	9..101	CDD:254352
		Ig	26..100	CDD:143165
		I-set	119..210	CDD:254352
		Ig	136..209	CDD:143165
		I-set	228..320	CDD:254352
		IGc2	242..312	CDD:197706
		I-set	335..425	CDD:254352
		Ig	350..423	CDD:143165
		Ig	436..530	CDD:299845
		I-set	438..532	CDD:254352
		I-set	545..636	CDD:254352
		Ig	560..633	CDD:143165
		Ig	657..731	CDD:143165
		I-set	746..835	CDD:254352
		Ig	1496..1586	CDD:299845
		IG_like	1503..1588	CDD:214653
		I-set	1639..1724	CDD:254352
		IG	1750..1821	CDD:214652
		IGc2	1750..1806	CDD:197706
		I-set	1826..1909	CDD:254352
		Ig	1831..1897	CDD:299845
		I-set	1914..1998	CDD:254352
		Ig	2020..2095	CDD:299845
		FN3	2100..2194	CDD:238020
		FN3	2203..2292	CDD:238020
		I-set	2311..2395	CDD:254352
		Ig	2321..2395	CDD:299845
		FN3	2399..2493	CDD:238020
		FN3	2501..2590	CDD:238020
		I-set	2602..2693	CDD:254352
		Ig	2619..2693	CDD:299845
		FN3	2697..2793	CDD:238020
		FN3	2801..2893	CDD:238020
		I-set	2902..2995	CDD:254352
		Ig	2919..2993	CDD:299845
		FN3	2999..3088	CDD:238020
		FN3	3102..3191	CDD:238020
		I-set	3208..3292	CDD:254352
		Ig	3219..3292	CDD:299845
		FN3	3296..3390	CDD:238020
		FN3	3400..3489	CDD:238020
		I-set	3494..3590	CDD:254352
		Ig	3516..3590	CDD:299845
		FN3	3594..3682	CDD:238020
		FN3	3694..3781	CDD:238020
		I-set	3795..3885	CDD:254352
		Ig	3813..3885	CDD:299845
		FN3	3889..3980	CDD:238020
		FN3	3990..4082	CDD:238020
		I-set	4090..4181	CDD:254352
		Ig	4108..4181	CDD:299845
		FN3	4185..4271	CDD:238020
		FN3	4285..4383	CDD:238020
		Ig	4404..4480	CDD:299845
		FN3	4484..4571	CDD:238020
		FN3	4584..4677	CDD:238020
		I-set	4685..4775	CDD:254352
		Ig	4703..4775	CDD:299845
		FN3	4779..4863	CDD:238020
		FN3	4879..4970	CDD:238020
		IG	4989..5069	CDD:214652
		Ig	4996..5069	CDD:299845
		FN3	5073..5166	CDD:238020
		FN3	5175..5265	CDD:238020
I-set	5276..5366	CDD:254352
Ig	5293..5366	CDD:299845
FN3	5370..5462	CDD:238020
FN3	5470..5561	CDD:238020
Ig	5559..5654	CDD:299845
I-set	5570..5660	CDD:254352
FN3	5664..5755	CDD:238020
FN3	5764..5853	CDD:238020
I-set	5865..5954	CDD:254352
Ig	5882..5954	CDD:299845
FN3	5958..6049	CDD:238020
FN3	6062..6153	CDD:238020
I-set	6173..6255	CDD:254352
Ig_Titin_like	6182..6255	CDD:143225
FN3	6259..6350	CDD:238020
FN3	6359..6451	CDD:238020
FN3	6470..6552	CDD:238020	23/82 (28%)
I-set	6564..6650	CDD:254352	11/85 (13%)
Ig_Titin_like	6577..6650	CDD:143225	9/72 (13%)
FN3	6654..6745	CDD:238020	17/93 (18%)
FN3	6754..6840	CDD:238020	19/107 (18%)
I-set	6855..6946	CDD:254352	16/103 (16%)
Ig	6874..6946	CDD:299845	14/84 (17%)
FN3	6950..7042	CDD:238020	21/95 (22%)
FN3	7050..7139	CDD:238020	23/112 (21%)
FN3	7151..7237	CDD:238020	13/85 (15%)
I-set	7254..7334	CDD:254352	17/125 (14%)
Ig	7272..7339	CDD:143165	12/86 (14%)
IG_like	7354..7440	CDD:214653	18/117 (15%)
Ig	7366..7440	CDD:299845	12/73 (16%)
FN3	7444..7531	CDD:238020	27/158 (17%)
FN3	7545..7637	CDD:238020	16/96 (17%)
I-set	7648..7737	CDD:254352	32/91 (35%)
Ig	7665..7734	CDD:143165	25/71 (35%)
Ig	7760..7833	CDD:299845	2/72 (3%)
FN3	7837..7927	CDD:238020	5/89 (6%)
STKc_Twitchin_like	7986..8244	CDD:271016	91/258 (35%)
S_TKc	7989..8244	CDD:214567	91/255 (36%)
Ig	8311..8394	CDD:299845
I-set	8312..8401	CDD:254352
I-set	8437..8525	CDD:254352
Ig	8455..8519	CDD:143165
I-set	8632..8721	CDD:254352
IGc2	8646..8711	CDD:197706
I-set	8740..8831	CDD:254352
Ig	8757..8826	CDD:143165
I-set	8839..8922	CDD:254352
Ig	8845..>8909	CDD:299845
Trio	NP_001074771.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..27
		SEC14	69..204	CDD:214706
		Spectrin 1	218..338
		SPEC	220..445	CDD:238103
		Spectrin 2	340..446
		SPEC	341..566	CDD:238103
		Spectrin 3	566..672
		SPEC	567..783	CDD:238103
		Spectrin 4	673..784
		SPEC	787..1014	CDD:238103
		Spectrin 5	907..1012
		SPEC	908..1137	CDD:238103
		Spectrin 6	1138..1244
		SPEC	1143..1243	CDD:197544
		RhoGEF	1295..1465	CDD:238091
		PH1_Kalirin_Trio_like	1472..1594	CDD:270060	11/39 (28%)
		PH	1505..1590	CDD:278594	11/35 (31%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1601..1651		16/86 (19%)
		SH3_Kalirin_1	1659..1718	CDD:212786	15/77 (19%)
		SH3-RhoG_link	1715..1972	CDD:293215	67/368 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1781..1906		31/171 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1927..1957		8/29 (28%)
		RhoGEF	1970..2143	CDD:238091	40/220 (18%)
		PH2_Kalirin_Trio_p63RhoGEF	2148..2286	CDD:270061	19/144 (13%)
		PH	2175..2271	CDD:278594	15/102 (15%)
		SH3_Kalirin_2	2559..2617	CDD:212787	9/57 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2643..2666		7/23 (30%)
		I-set	2689..2780	CDD:254352	32/91 (35%)
		Ig	2703..2780	CDD:299845	27/77 (35%)
		STKc_Trio_C	2792..3054	CDD:271015	97/387 (25%)
		S_TKc	2800..3054	CDD:214567	91/255 (36%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910