DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ekar and Grid2

DIOPT Version :9

Sequence 1:	NP_001036250.1	Gene:	Ekar / 43806	FlyBaseID:	FBgn0039916	Length:	899	Species:	Drosophila melanogaster
Sequence 2:	NP_077355.2	Gene:	Grid2 / 79220	RGDID:	68368	Length:	1007	Species:	Rattus norvegicus

Alignment Length:	870	Identity:	240/870 - (27%)
Similarity:	434/870 - (49%)	Gaps:	105/870 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly    22 LVRIGAIFSNQPGMYNSELAFRYAIHRLNMDKSLLPETTVDYYVEYVNRFDSFETVQKVCKLIRV 86
            ::.||||| ::....:.|: ||.|:..||.::.:|....:.:.|.:|:..:.|:.||:.|:|:..
  Rat    26 IIHIGAIF-DESAKKDDEV-FRTAVGDLNQNEEILQTEKITFSVTFVDGNNPFQAVQEACELMNQ 88

  Fly    87 GVQAVFSPTDSVLATHINSICDALDIPN--IGRSA----------------HDFSINVYPSKQLV 133
            |:.|:.|......|..:.|:.||:.||:  |.||.                .|::::|.|...|.
  Rat    89 GILALVSSIGCTSAGSLQSLADAMHIPHLFIQRSTAGTPRSGCGLTRSNRNDDYTLSVRPPVYLN 153

  Fly   134 NYAFNDVIQYLNWTRFGILHEKENGIINLHQL--SRSFHG-EVHMRQVSRD-----------SYV 184
            ......|.:|. |.:|.|.::.|..|..:.:.  ..|..| :|.:::|..:           ..:
  Rat   154 EVILRVVTEYA-WQKFIIFYDSEYDIRGIQEFLDKVSQQGMDVALQKVENNINKMITTLFDTMRI 217

  Fly   185 SALNEFKGKEIHNIIIDTNSNGISILLKNILQQQMNEYKYHYLFTSFDLETYDLEDFKYNFVNIT 249
            ..||.:: ..:...|:..|.......:..:::..:..:..|::..:.::...|:::
  Rat   218 EELNRYR-DTLRRAILVMNPATAKSFISEVVETNLVAFDCHWIIINEEINDVDVQE--------- 272

  Fly   250 SFRLVDTADVGVKQILKDIGLYSHHIFKKPYLNLHIKKSTI----------LESEPALMFDSVYV 304
               || ...:|...|::.......:|.::.:...|...||:          :|.....::|:|.:
  Rat   273 ---LV-RRSIGRLTIIRQTFPVPQNISQRCFRGNHRISSTLCDPKDPFAQNMEISNLYIYDTVLL 333

  Fly   305 FAIGL-QTLEQSHSLTLLNISCEEENS--WDGGLSLINYLNAVEWKGLTGPIQF-KDGQRVQFKL 365
            .|... :.||.....::.::||..:||  |.||.|::..:......||||.::| ::|.......
  Rat   334 LANAFHKKLEDRKWHSMASLSCIRKNSKPWQGGRSMLETIKKGGVNGLTGDLEFGENGGNPNVHF 398

  Fly   366 DLIKLKQ-----HSIVKVGEWTPHGHLNITEPSMFFDAGSMNVTLVVITILETPYVMMHYGKNFT 425
            :::....     ..:.|:|.|.|...||.:......:.....|.|.|:|:||.|:||:  .:|..
  Rat   399 EILGTNYGEELGRGVRKLGCWNPVTGLNGSLTDKKLENNMRGVVLRVVTVLEEPFVMV--SENVL 461

  Fly   426 GN-ERFYGFCVDILETISREVGFDYILDLVPDRKYGAKDPETGEWNGMVAQLMKYKADLAVGSMT 489
            |. :::.||.:|:|:.:|..:||:|.:.:.||.|||:.. |.|.|||:|.:|:..:||:.:.::|
  Rat   462 GKPKKYQGFSIDVLDALSNYLGFNYEIYVAPDHKYGSPQ-EDGTWNGLVGELVFKRADIGISALT 525

  Fly   490 ITYARESVIDFTKPFMNLGISILFKVPTSEPT-RLFSFMNPLAIEIWIYVLIAYFLVSLCIYIVG 553
            ||..||:|:|||..:|:..:.:|.:  .:|.| .:|:.:.|..:.:|..:.....||.|.:|::.
  Rat   526 ITPDRENVVDFTTRYMDYSVGVLLR--RAEKTVDMFACLAPFDLSLWACIAGTVLLVGLLVYLLN 588

  Fly   554 KLSPIEWKCINACDLENISIGNQFSLT--DSFWFTIGTFMQQSPDIYPRAMSTRIISSTWGFFSL 616
            .|:|           ..:.:|:..|.|  :|.||..|:|:||..::....::||::...|..|:|
  Rat   589 WLNP-----------PRLQMGSMTSTTLYNSMWFVYGSFVQQGGEVPYTTLATRMMMGAWWLFAL 642

  Fly   617 IIVASYTANLAAFLTTERMINPIENAEDLASQTEISYGT-LDSG--------STMTFFRDSVIET 672
            |:::|||||||||||..|:.:.|::.:||:.||:|.||| |||.        ....|.|||:
  Rat   643 IVISSYTANLAAFLTITRIESSIQSLQDLSKQTDIPYGTVLDSAVYQHVRMKGLNPFERDSM--- 704

  Fly   673 YKKIWRSMDNKKPSAFTTTYED--GIKRVNQGNYAFLMESTMLDYIV--QRDCNLTQIGGLLDTK 733
            |.::|| |.|:...:.....|.  ||::|..|||||:.::.:|:|:.  ..||:...:|..:..:
  Rat   705 YSQMWR-MINRSNGSENNVLESQAGIQKVKYGNYAFVWDAAVLEYVAINDPDCSFYTVGNTVADR 768

  Fly   734 GYGIATPKGSPWRDKISLAILELQERGDIQMLYDKWWKNTDETCTRKNTSKQSKANSLGLESIGG 798
            |||||...|||:||..|..|||||:.||:.:|..|||....:.....:...:.|..:|.::|:.|
  Rat   769 GYGIALQHGSPYRDVFSQRILELQQSGDMDILKHKWWPKNGQCDLYSSVDAKQKGGALDIKSLAG 833

  Fly   799 VFVVLIAGIIVAAVVAFFEFWYNFR 823
            ||.:|.|||:::.::|..|.|::.|
  Rat   834 VFCILAAGIVLSCLIAVLETWWSRR 858

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ekar	NP_001036250.1	PBP1_iGluR_Kainate	24..388	CDD:107377	84/414 (20%)
		ANF_receptor	39..371	CDD:279440	75/377 (20%)
		PBP2_iGluR_Kainate	402..771	CDD:270432	139/385 (36%)
		Lig_chan	533..807	CDD:278489	100/288 (35%)
Grid2	NP_077355.2	Interaction with CBLN1 homotrimer. /evidence=ECO:0000250\|UniProtKB:O43424	24..345		66/335 (20%)
		Periplasmic_Binding_Protein_type1	28..429	CDD:415822	86/417 (21%)
		PBP2_iGluR_delta_2	440..806	CDD:270449	139/385 (36%)
		Interaction with AP4M1. /evidence=ECO:0000269\|PubMed:14572453	921..991
		PDZ-binding. /evidence=ECO:0000250	1005..1007
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1052
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000021
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR18966
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	5	4.910