DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ekar and clumsy

DIOPT Version :9

Sequence 1:	NP_001036250.1	Gene:	Ekar / 43806	FlyBaseID:	FBgn0039916	Length:	899	Species:	Drosophila melanogaster
Sequence 2:	NP_001036373.1	Gene:	clumsy / 35394	FlyBaseID:	FBgn0026255	Length:	1002	Species:	Drosophila melanogaster

Alignment Length:	979	Identity:	318/979 - (32%)
Similarity:	495/979 - (50%)	Gaps:	160/979 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 LIASGFLLFQFLSYGLGVPPLV-------RIGAIFSNQPGMYNSELAFRYAIHRLNM---DKSLL 56
            |..:.|||       :.:|.|.       .:|:||::...  .||:|||.|:.|.|:   :..|:
  Fly     4 LFLTHFLL-------IALPVLADIDRSQFMVGSIFTSDKD--ESEIAFRTAVDRANILERNVELV 59

  Fly    57 PETTVDYYVEYVNRFDSFETVQKVCKLIRVGVQAVFSPTDSVLATHINSICDALDIPNI------ 115
            |      .|.|.|..|||...:.||.||..||.|:|.|:....:..|.||||.||||:|
  Fly    60 P------IVVYANTDDSFIMEKMVCNLISQGVIAIFGPSTGSSSDIIASICDTLDIPHIVYDWIP 118

  Fly   116 -----GRSAHDFSINVYPSKQLVNYAFNDVIQYLNWTRFGILHEKENGIINLH---QLSRSFHGE 172
                 .|.....::||:|...|::....:::|...|..|.:::|.:..:..|.   |:.......
  Fly   119 NESIPDREHSTMTLNVHPDNLLLSQGLAEIVQSFAWRSFTVVYETDKELQQLQDILQVGEPISNP 183

  Fly   173 VHMRQVS-RDSYVSALNEFKGKEIHNIIIDTNSNGISILLKNILQQQMNEYK----YHYLFTSFD 232
            ..::|:. .|.:...|.|.|....:.:|:....:.   |||  :.||.||.|    |..:|...
  Fly   184 TTVKQLGPGDDHRPFLKEIKLSTDNCLILHCAPDN---LLK--ILQQANELKMLGEYQSVFIPL- 242

  Fly   233 LETYDLEDFKYNFV--NITSFRLVDTADVGVKQILKD-------IGLYSHHIFKKPYLNLHIKKS 288
            |:|:.::..:.:.|  |||:.||:|.:|..||.::.|       .|.|    ||       :..:
  Fly   243 LDTHSIDFGELSGVEANITTVRLMDPSDFHVKNVVHDWEEREKREGRY----FK-------VDPN 296

  Fly   289 TILESEPALMFDSVYVFAIGLQTLEQSHSLTLLNISCEEENSWDGGLSLINYLNAVEWKGLTGPI 353
            .: :|:..|:.|:|::|:.||..|.....||..::.|..:..|..|..:|.::.|...:..||.|
  Fly   297 RV-KSQMILLNDAVWLFSKGLTELGIFEELTAPDLECRRKKPWPFGKRIIEFIKARSEETSTGRI 360

  Fly   354 QF-KDGQRVQFKLDLIKLKQHSIVKVGEWTPHGHLNI----TEPSMFFDAGSMNVTLVVITILET 413
            .| ::|||..|.|..::|.....:.:..|.|...|::    .|..........|.|.:|.:.|..
  Fly   361 DFNENGQRSFFTLRFMELNSDGFLDLATWDPVNGLDVLNDDEESEKRVGQKLSNKTFIVSSRLGA 425

  Fly   414 PYVMM---HYGKNFTGNERFYGFCVDILETISREVGFDYILDLVPDRKYGAKDPETGEWNGMVAQ 475
            |::.:   ..|:..|||.|:.|:.:|::..|::.:.|.:...:.||.||||.:..|..|:|:|.|
  Fly   426 PFLTLREPQEGEILTGNSRYEGYSIDLINEIAKMLNFKFEFRMSPDGKYGALNKVTQTWDGIVRQ 490

  Fly   476 LMKYKADLAVGSMTITYARESVIDFTKPFMNLGISILFKVPTSEPTRLFSFMNPLAIEIWIYVLI 540
            |:...|||.:..:|:|.:|...:|||.|||.|||||||..|.:.||.||||::|.::::|||:..
  Fly   491 LIDGNADLGICDLTMTSSRRQAVDFTPPFMTLGISILFSKPPTPPTDLFSFLSPFSLDVWIYMGS 555

  Fly   541 AYFLVSLCIYIVGKLSPIEWKCINAC----DLENISIGNQFSLTDSFWFTIGTFMQQSPDIYPRA 601
            ||..:||.::.:.:::|.:|:..:.|    ::|||     :|:.::.|.:||:.|.|..||.|:|
  Fly   556 AYLFISLLLFALARMAPDDWENPHPCKEPEEVENI-----WSIMNTTWLSIGSLMGQGCDILPKA 615

  Fly   602 MSTRIISSTWGFFSLIIVASYTANLAAFLTTERMINPIENAEDLASQTEISYGTLDSGSTMTFFR 666
            .|||:::..|.||:|:::.|||||||||||..|..|.|.:|||||:|::|.||.:..||||.|||
  Fly   616 ASTRLVTGMWWFFALMMLNSYTANLAAFLTNSRQANSINSAEDLAAQSKIKYGAMAGGSTMGFFR 680

  Fly   667 DSVIETYKKIWRSMDNKKPSAFTTTYEDGIKRVNQGN--YAFLMESTMLDYIVQRDCNLTQIGGL 729
            ||...||:|:|.:|::..||.||.|.::|::||.:|.  ||||||||.|:|.|:|.|:|.||||.
  Fly   681 DSNFSTYQKMWTAMESASPSVFTKTNDEGVERVQKGKNLYAFLMESTTLEYNVERKCDLVQIGGW 745

  Fly   730 LDTKGYGIATPKGSPWRDKISLAILELQERGDIQMLYDKWWK--NTDETCTRKNTSKQSKANSLG 792
            ||.|.||||.|..||:|.:||.|:|:|.|.|.:..|..||||  :....| .|:.........||
  Fly   746 LDYKSYGIAMPFNSPYRKQISAAVLKLGELGQLAELKRKWWKEMHGGGNC-EKSDEDGGDTPELG 809

  Fly   793 LESIGGVFVVLIAGIIVAAVVAFFEFWYNFR-------------YNYEA--------------TP 830
            ||::||||:||..|::.|.|:...||.:|.:             :..||              |.
  Fly   810 LENVGGVFLVLGLGLLSAMVLGCTEFLWNVKSVAIEEKISLKEAFKSEALFAARIWITTKPVHTS 874

  Fly   831 SQSVVNN-----------KYNQDGI-----------------------------LESERNYTPPD 855
            |:|..:|           .:...|:                             |:|::..|||.
  Fly   875 SESGSSNSSSSSSSRSKHSFKSQGLSMKSLKSSGYQDVEASVHSKLKKIGSMFSLKSQKTVTPPP 939

  Fly   856 RSFW 859
            ...|
  Fly   940 EIGW 943

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ekar	NP_001036250.1	PBP1_iGluR_Kainate	24..388	CDD:107377	109/395 (28%)
		ANF_receptor	39..371	CDD:279440	102/363 (28%)
		PBP2_iGluR_Kainate	402..771	CDD:270432	168/377 (45%)
		Lig_chan	533..807	CDD:278489	129/281 (46%)
clumsy	NP_001036373.1	PBP1_iGluR_Kainate	26..396	CDD:107377	109/395 (28%)
		ANF_receptor	39..379	CDD:279440	102/363 (28%)
		PBP2_iGluR_Kainate	414..787	CDD:270432	168/377 (45%)
		Lig_chan	548..812	CDD:278489	122/269 (45%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45462691
Domainoid	1	1.000	82	0.746	Domainoid score	I2907
eggNOG	1	0.900	-	-		E1_KOG1052
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S6275
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D60688at33208
OrthoFinder	1	1.000	-	-		FOG0000021
OrthoInspector	1	1.000	-	-		mtm1027
orthoMCL	1	0.900	-	-		OOG6_100167
Panther	1	1.100	-	-	P	PTHR18966
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	10	9.700