DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ekar and Grin3a

DIOPT Version :9

Sequence 1:	NP_001036250.1	Gene:	Ekar / 43806	FlyBaseID:	FBgn0039916	Length:	899	Species:	Drosophila melanogaster
Sequence 2:	NP_001263284.1	Gene:	Grin3a / 242443	MGIID:	1933206	Length:	1135	Species:	Mus musculus

Alignment Length:	929	Identity:	201/929 - (21%)
Similarity:	362/929 - (38%)	Gaps:	195/929 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    41 AFRYAIHRLNMDKSLLPETTVDYYVEYVNRF---------------------DSFETVQKVCKLI 84
            |..:|:..||..:.|||   .:..:|.|...                     |.|..:|.||..:
Mouse   127 ALLFAVENLNRVEGLLP---YNLSLEVVMAIEAGLGDLPLMPFSSPSSPWSSDPFSFLQSVCHTV 188

  Fly    85 RV-GVQAVFS-PTDSVLATHINSICDALDIPNIGRSAHDF---SIN-VYPSKQLVNYAFND---- 139
            .| ||.|:.: |........::.:...|.||.:....|:|   |.| ::....|.|...:|
Mouse   189 VVQGVSALLAFPQSQGEMMELDLVSSVLHIPVLSIVRHEFPRESQNPLHLQLSLENSLSSDADVT 253

  Fly   140 --VIQYLNWTRFGILHEKENGIIN----LHQLSRSFHGEVHMRQVSRDSYVSALNEFKGKEIHNI 198
              ::...||..|.:|..:|:..|.    |.:.:..||.|..:...:..|....|..|...::.||
Mouse   254 VSILTMNNWYNFSLLLCQEDWNITDFLLLTENNSKFHLESIINITANLSSTKDLLSFLQVQLENI 318

  Fly   199 IIDTNSNGISIL-------LKNILQQQMNEYKYHYLFTSFDLETYDLEDFKYNFVNITSFRLVDT 256
               .||....::       ::.|.:          :.|.|.|...||.....:..|:...|....
Mouse   319 ---RNSTPTMVMFGCDMGSIRQIFE----------MSTQFGLSPPDLHWVLGDSQNVEELRTEGL 370

  Fly   257 ADVGVKQILKDIGLYSHHIFKKPYLNLHIKKSTILESEPALMFDSVYVFAIGLQTLEQSHSLTLL 321
                      .:||.:|....:.....:::.:..|.:.           |:...|:.|. .|.||
Mouse   371 ----------PLGLIAHGKTTQSVFEYYVQDAMELVAR-----------AVATATMIQP-ELALL 413

  Fly   322 --NISCEEENSWD--GGLSLINYLNAVEWKGLTGPIQFKDGQRVQ-------FKLDLIKLKQHSI 375
              .::|.:..:.:  .|..|..:|....::||:|.|:.|....|.       :.|....:.:...
Mouse   414 PSTMNCMDVKTTNLTSGQYLSRFLANTTFRGLSGSIKVKGSTIVSSENNFFIWNLQYDPMGKPMW 478

  Fly   376 VKVGEWTPHGHLNITEPSMFFDAGSMNVT---------LVVITILETPYVMMH------------ 419
            .::|.|  .|...:.:..::.:....:.|         |.|:|::|.|:|...
Mouse   479 TRLGSW--QGGRIVMDSGIWPEQAQRHKTHFHHPNKLHLRVVTLIEHPFVFTREVDDEGLCPAGQ 541

  Fly   420 --------------------YGKNFTGNERF----YGFCVDILETISREVGFDYILDLVPDRKYG 460
                                :..|.|...:|    ||:|:|:||.::.::.||:.|.:|.|.|||
Mouse   542 LCLDPMTNDSSILDSLFSSLHSSNDTVPIKFKKCCYGYCIDLLEQLAEDMNFDFDLYIVGDGKYG 606

  Fly   461 AKDPETGEWNGMVAQLMKYKADLAVGSMTITYARESVIDFTKPFMNLGISILFKV-PTSEPTRLF 524
            |.  :.|.|.|:|..|:...|::||.|.:|..||..|||||.||.:..:.||.:. .|:.|  :.
Mouse   607 AW--KNGHWTGLVGDLLSGTANMAVTSFSINTARSQVIDFTSPFFSTSLGILVRTRDTAAP--IG 667

  Fly   525 SFMNPLAIEIWIYVLIAYFLVSLCIYIVGKLSPIEWKCINACDLENISIGNQFSLTDS----FWF 585
            :||.||...:|:.:.:|..:.::.      |:..|||.......:..:....||.:.:    :..
Mouse   668 AFMWPLHWTMWLGIFVALHITAIF------LTLYEWKSPFGMTPKGRNRNKVFSFSSALNVCYAL 726

  Fly   586 TIGTFMQQSPDIYPRAMSTRIISSTWGFFSLIIVASYTANLAAFLTTERMINPIENAED--LASQ 648
            ..|......|   |:..:.|.:.:.|..|.:..:::|||||||.:..|::...:....|  |...
Mouse   727 LFGRTAAIKP---PKCWTGRFLMNLWAIFCMFCLSTYTANLAAVMVGEKIYEELSGIHDPKLHHP 788

  Fly   649 TE-ISYGTLDSGSTMTFFRDSVIETYKKIWRSMDNKKPSAFTTTYEDGIKRVNQGNY---AFLME 709
            :: ..:||:...|...:.|.|..|.::.:.|......|        ||::.:.....   ||:|:
Mouse   789 SQGFRFGTVRESSAEDYVRQSFPEMHEYMRRYNVPATP--------DGVQYLKNDPEKLDAFIMD 845

  Fly   710 STMLDY--IVQRDCNLTQIGGLLDTKGYGIATPKGSPWRDKISLAILELQERGDIQMLYDKWWKN 772
            ..:|||  .:..||.|..:|.....:||||..|..||....||..|.:.:..|.:.:|:|||:|.
Mouse   846 KALLDYEVSIDADCKLLTVGKPFAIEGYGIGLPPNSPLTSNISELISQYKSHGFMDVLHDKWYKV 910

  Fly   773 TDETCTRKNTSKQSKANSLGLESIGGVFVVLIAGIIVAAVVAF------------------FEFW 819
            .  .|.:::.: .::...:|::...|:||:|..|..::.:...                  .::|
Mouse   911 V--PCGKRSFA-VTETLQMGIKHFSGLFVLLCIGFGLSILTTIGEHIVYRLLLPRIKNKSKLQYW 972

  Fly   820 YNFRYNYEATPSQSVVNNK 838
            .:....:....:.|.|..|
Mouse   973 LHTSQRFHRALNTSFVEEK 991

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ekar	NP_001036250.1	PBP1_iGluR_Kainate	24..388	CDD:107377	78/401 (19%)
		ANF_receptor	39..371	CDD:279440	75/384 (20%)
		PBP2_iGluR_Kainate	402..771	CDD:270432	111/426 (26%)
		Lig_chan	533..807	CDD:278489	66/285 (23%)
Grin3a	NP_001263284.1	PBP1_iGluR_NMDA_NR3	30..497	CDD:107372	78/409 (19%)
		PBP2_iGluR_NMDA_Nr3	512..908	CDD:270438	109/416 (26%)
		Lig_chan	676..942	CDD:278489	66/285 (23%)
		PPP2CB binding site. /evidence=ECO:0000250\|UniProtKB:Q9R1M7	951..987		1/35 (3%)
		GIT1-binding. /evidence=ECO:0000250\|UniProtKB:Q9R1M7	1082..1115
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167846527
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1052
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.740