DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Eph and epha4b

DIOPT Version :9

Sequence 1:	NP_524625.3	Gene:	Eph / 43803	FlyBaseID:	FBgn0025936	Length:	1096	Species:	Drosophila melanogaster
Sequence 2:	NP_705944.1	Gene:	epha4b / 64270	ZFINID:	ZDB-GENE-030826-6	Length:	976	Species:	Danio rerio

Alignment Length:	1040	Identity:	437/1040 - (42%)
Similarity:	598/1040 - (57%)	Gaps:	132/1040 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly    83 DQVVLLDT-TREATLEWTRYPYGPQAQTPGWVEESFTDFVKGINWRSYVVCDVAYHNVNNWLWSP 146
            ::|.|||| :....|.|..||      ..||.|.|..| .:....|||.||:|...:.|||..:.
Zfish    30 NEVTLLDTMSALGDLGWEAYP------AEGWEEISVMD-ERNTPMRSYQVCNVMEASQNNWFRTR 87

  Fly   147 FIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDAATREPPPW--QTDSYRLIARI 209
            .|.|..|.|:||||:||:|||:..||...:|||||::.:||    :..|..|  :...|..|..|
Zfish    88 HIAREGAQRVYIEIKFTLRDCNSLPGVPGTCKETFNMYYYE----SNNPNLWFIKESQYIKIDTI 148

  Fly   210 AAGEGRFNQNSDVD----INTEVKSI-AVNKKGVYFAFRDQGACISVLAVKVYYITCPAVTENFA 269
            ||.|. |.|....|    :|||::.| .::|||.|.||:|.||||::::::|:|..||....|.|
Zfish   149 AADES-FTQTDVGDRVMRLNTEIRDINNLSKKGFYLAFQDVGACIALVSLRVFYKKCPLAVLNLA 212

  Fly   270 HFNETPTGREITIIEKQNGTCVDNAEPYETPTYLCKGDGKWTILTGGCRCKAGYEPNYTNKTCTE 334
            .|.:|.||.:..::|.: |:||:::|.:|.|...|.|||.|.:..|.|.||.|:|.|  ...|..
Zfish   213 QFPDTVTGGDSALVEVR-GSCVNDSEEFEAPRMYCSGDGGWLVPIGRCVCKPGFEEN--KDYCQA 274

  Fly   335 CPLGTFKSPEV-TKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPCYSPPAAPTNLTLLFV 398
            |..||:|:..: ..|:.|||:|.:.:..:..|.|..||||...|.|.|.|..||:||.| .:..:
Zfish   275 CRPGTYKASSMDAYCSKCPPHSFSHQEKASECSCEKGFYRADMDPRSMACTRPPSAPEN-PISTL 338

  Fly   399 DQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKIKC-------NICSP---NVVYNPSTDTFNE 453
            :.||..:.||.|..:..          ..|:.|.|.|       .||||   ||.:.|.....:.
Zfish   339 NDTSVTLEWSPPRDSGG----------RGDVTYSIHCRKCAGDGRICSPCSNNVHFVPRQFGLSI 393

  Fly   454 TKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLLNIPLDLNEVKTG 518
            .|:.:|:|...|.||..:.|:|.||.::     .....||.|:
Zfish   394 PKVLITDLLSNTNYTFSVEAVNGVSDLS-----PTPKQLVTVN---------------------- 431

  Fly   519 QAEIVFTTESVLLSTVFNLRILAITNKDA-DLEWDKPVQSDFPLEFYEVRWFPKVELDAINKSAL 582
                :.|:::|      |:.:....:||: .|.|..|.:.:..:..|||.::.|.:.|. |.:.|
Zfish   432 ----ITTSQTV------NVILKERKSKDSITLAWQGPDRPNGVIVEYEVIYYEKNQRDQ-NYTVL 485

  Fly   583 NTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQT----LQSVGSVYDDSVQIRFIAGA 643
            .||.....:.||...|.|.|:||.:|:.|:|:|...|..:|    :.::|.....::....|||.
Zfish   486 KTKGHMMTVEGLKPGTTYVFRVRARTDGGYGNYGGEIELETSHEDMLAIGGPNQSTILAVSIAGG 550

  Fly   644 IVTGVLFLVIFIIATVY--FMRSKHQDDLDKK--STNHLPLPLDYASNEVNSMDTTPIVKKLHLN 704
            ||.....:..|:::..:  ::::|...|.:|.  ....:.||
Zfish   551 IVLLTFLVACFVVSGRHCGYIKAKQDPDEEKMQFQNGRVKLP----------------------- 592

  Fly   705 VTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRGRLKIPPNFVQD 769
                   .||:|:.||||||||||:|:||:||||:.|.||.:||.||||:||.||||:|..  ::
Zfish   593 -------GSRTYIHPHTYEDPNQAVRDFAKEIDASTIRIERVIGAGEFGEVCSGRLKLPSK--RE 648

  Fly   770 IDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITEYMENGSLDTFLR 834
            |.||||:||.|.|||.|.|||:||||||||||||:|.|:|||||..||||||||||||||||||:
Zfish   649 IYVAIKSLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTRCKPVMIITEYMENGSLDTFLK 713

  Fly   835 VNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFGLSREIENASD- 898
            .:||:|..:||:.|:|||||||.|||||:||||||||||:|||:.|:||::||||||.:|:..:
Zfish   714 KHDGQFTVIQLVGMMRGIASGMKYLSDMSYVHRDLAARNILVNSNLVCKVSDFGLSRVLEDDPEA 778

  Fly   899 AYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWSNQDVIKSIEKGYR 963
            ||||||||||:|||||||||:|||||||||||||:|:|||:||||||||..|||||||:|::|||
Zfish   779 AYTTRGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVISYGERPYWEMSNQDVIKAIDEGYR 843

  Fly   964 LPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTTRPSPESDGNHILDGQR 1028
            ||||||||..|:|||||||:|.|:.||.|..||:|||.|.|.|.||      .|...:.:.:...
Zfish   844 LPAPMDCPVVLHQLMLDCWEKSRSERPKFGQIVNTLDKLIRNPNSL------KELANSSVWEDPT 902

  Fly  1029 GQNIFIST-DLWLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDMGITLVGHQKKILHQARQL 1092
            .....:|| |.||:.|.|.:|..:|..|..::...|..::..:||.|.:||||||||||...:.|
Zfish   903 TPEFSVSTVDEWLDAINMGQYKDNFANAGYVSLDSILYISMCELSKMDVTLVGHQKKILSAVQSL 967

Fly 1093 1092
Zfish 968 967

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	73/182 (40%)
		FN3	387..476	CDD:238020	27/98 (28%)
		FN3	536..623	CDD:238020	27/87 (31%)
		EphA2_TM	644..736	CDD:291255	24/95 (25%)
		PTKc_EphR	736..1002	CDD:270629	193/266 (73%)
		Pkinase_Tyr	741..999	CDD:285015	187/258 (72%)
		SAM_1	1033..1092	CDD:278937	24/59 (41%)
		SAM	1039..1092	CDD:197735	21/52 (40%)
epha4b	NP_705944.1	EphR_LBD_A	31..203	CDD:198441	73/183 (40%)
		FN3	328..434	CDD:238020	33/147 (22%)
		FN3	447..526	CDD:238020	26/79 (33%)
		EphA2_TM	546..617	CDD:291255	27/100 (27%)
		PTKc_EphR_A	617..883	CDD:270651	193/267 (72%)
		Pkinase_Tyr	622..879	CDD:285015	187/258 (72%)
		SAM_EPH-R	907..967	CDD:188887	24/59 (41%)
		SAM	907..967	CDD:197735	24/59 (41%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	396	1.000	Domainoid score	I723
eggNOG	1	0.900	-	-		E2759_KOG0196
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	753	1.000	Inparanoid score	I540
OMA	1	1.010	-	-		QHG45908
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	1	1.000	-	-		FOG0000086
OrthoInspector	1	1.000	-	-		otm24639
orthoMCL	1	0.900	-	-		OOG6_100189
Panther	1	1.100	-	-	O	PTHR24416
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X82
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	12	11.880