DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Eph and epha2b

DIOPT Version :9

Sequence 1:	NP_524625.3	Gene:	Eph / 43803	FlyBaseID:	FBgn0025936	Length:	1096	Species:	Drosophila melanogaster
Sequence 2:	XP_005162266.1	Gene:	epha2b / 568718	ZFINID:	ZDB-GENE-030219-187	Length:	979	Species:	Danio rerio

Alignment Length:	1073	Identity:	416/1073 - (38%)
Similarity:	583/1073 - (54%)	Gaps:	166/1073 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly    65 LRTILLIIISIHF---KLAHADQVVLLDTTREATLEWTRYPYGPQAQTPGWVEESFTDFVKGINW 126
            ::.:|.:.:.::|   .|...::|:|......|.|.|...|.     ..||  |.....|.|...
Zfish     6 IKRVLFVNVFMNFVFISLQKKEEVLLDMVASGAELGWLTSPV-----KDGW--EIGQQAVNGSLL 63

  Fly   127 RSYVVCDVAYHNVNNWLWSPFIDRG-SANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDA 190
            .:|.||:|.....:|||.:.||.|. :|:|:::|:||.:|||:.|....|:|||||:|...|.||
Zfish    64 YNYYVCNVEKGEQDNWLRTTFIQRNPAASRVFVELQFNVRDCNTFEAVPLNCKETFNLYTSEADA 128

  Fly   191 ----ATREPPPWQTDSYRLIARIAAGEGRFNQNSDVDINTEVKSI-AVNKKGVYFAFRDQGACIS 250
                |.|:      ..:|.:|.||..|  .:....:.:|||.|.: .:::||.|..|:|.|||::
Zfish   129 DIGTAFRK------GLFRKVATIAPDE--ISDRGKMKMNTETKVVDNLSRKGFYLGFQDIGACVA 185

  Fly   251 VLAVKVYYITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYLCKGDGKWTILTG 315
            :.:|:|||.||||..::.|.|.||..|.|...:.:..|:||.||.....|...|..||:|.:...
Zfish   186 IYSVRVYYKTCPATVQSLAAFPETVAGGENQALREVAGSCVKNAISEGQPRIYCTTDGEWVVPMS 250

  Fly   316 GCRCKAGYEPNYTNKTCTECPLGTFKSPEVTK--CTPCPPNSNASKTGSPFCKCASGFYRHPNDG 378
            .|:|::|:|.  ...||.||..|.|| |.|:.  |.|||.|:..|..|:..|.|..||||.|.|.
Zfish   251 QCQCESGFEA--LEDTCQECQPGFFK-PSVSSEACKPCPKNTEPSGPGATRCSCNDGFYRAPEDP 312

  Fly   379 RHMPCYSPPAAPTNLTLLFVDQTSAI------ISWSAPAKNESFSSETNSKIYHSDIVYKI---- 433
            ....|:..|:||.:|    :..|:.:      :||..||.|..          .|||.|.:
Zfish   313 ETSACFGLPSAPQSL----LSSTAQMSFGRLQLSWKPPADNGG----------RSDITYSVVCER 363

  Fly   434 ----KCNICSPNVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVA 494
                .|..|...|.:.||.....|||:|::.|||...||..:.|.:.||..:. ||        |
Zfish   364 CEGKTCQSCGEKVRFEPSNTELKETKVTVSELEPHLNYTFSVEARSGVSQFSH-KR--------A 419

  Fly   495 VSDIVFSNTSL--LNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWD----- 552
            ||.|   ||:|  .:.|      |.....:|.::.:.|                 .|:|.
Zfish   420 VSSI---NTTLHYTDPP------KVTAMHLVESSPTSL-----------------SLQWSVSHRA 458

  Fly   553 KPVQSDFPLEFYEVRWFPKVELDAINKSALNT-------KETKAHIVGLLENTEYGFQVRCKTNN 610
            |||.:.:.|      .|.|.|.|....|...|       ::..|.|..|..:|.|.|:|:..::.
Zfish   459 KPVATRYEL------MFRKKENDEEKASGTVTTFTVLVLEKNSAQIKDLSPSTVYLFKVQALSSE 517

  Fly   611 GF-GSYSNMIYAQTLQSVGSVYDDSVQIRFIAGAIVTGVLFLVIFIIATVYFMRSKHQDDLDKKS 674
            |. ||||......||...|:.       ..|.||.:.|.:.|  ||:..|..:|.:.::...::.
Zfish   518 GSPGSYSIEQEFSTLPPAGNT-------AVILGAAIGGAVML--FIVVVVLLLRKRRRNSHTRQG 573

  Fly   675 TNHLPLPLD-YASNEVNSMDTTPIVKKLHLNVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDA 738
                  |.| |.|:.               :...||    ::|||||||||||.|:.:||.||..
Zfish   574 ------PEDTYFSSP---------------DQLKPL----KTYVDPHTYEDPNTAVLKFASEIHP 613

  Fly   739 NYITIEAIIGGGEFGDVCRGRLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPN 803
            |:||.:.:||.||||:|.||.||.|..  :::.|||||||||.:||.|.|||:||||||||.|.|
Zfish   614 NHITKQKVIGAGEFGEVYRGILKAPSR--KEVAVAIKTLKPGYTEKQRQDFLSEASIMGQFSHQN 676

  Fly   804 VIYLQGVVTRSNPVMIITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRD 868
            :|.|:||||:....||:|||||||:||.:|:.:||:..:.||:.||||||:||.|||||:|||||
Zfish   677 IIRLEGVVTKFKHAMIVTEYMENGALDKYLKDHDGEMSSFQLVGMLRGIAAGMKYLSDMSYVHRD 741

  Fly   869 LAARNVLVNAQLICKIADFGLSREIENASD-AYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSYG 932
            |||||:|||:.|.||::||||||.:|:..: .|||.|||||:|||||||||:|||||||||||:|
Zfish   742 LAARNILVNSNLECKVSDFGLSRVLEDDPEGTYTTSGGKIPIRWTAPEAIAYRKFTSASDVWSFG 806

  Fly   933 VVLWEVMSYGERPYWNWSNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVS 997
            :|:||||::||||||:.||.:|:|:|.:.:|||||||||.|:|||||.|||:.|:.||.|..|||
Zfish   807 IVMWEVMAFGERPYWDMSNHEVMKAINEAFRLPAPMDCPSAVYQLMLQCWQQDRSKRPRFGDIVS 871

  Fly   998 TLDNLARQPQSL-----LTTRPS---PESDGNHILDGQRGQNIFISTDLWLEHIKMSRYCHHFKE 1054
            .||.|.:.|.||     ...|.|   |.:.|:   ||    :.|.|...|||.||||:|..:|..
Zfish   872 LLDKLLKSPDSLKPIADFDPRVSIRLPSTSGS---DG----SPFRSVSEWLESIKMSQYSENFSM 929

  Fly  1055 ANLINAQQISRLTAQQLSDMGITLVGHQKKILH 1087
            |.:::.:|:.::.::.:.::|:.|.||.|:|.:
Zfish   930 AGVVSMEQVLQMKSEDIRNIGVRLPGHLKRIAY 962

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	63/180 (35%)
		FN3	387..476	CDD:238020	29/102 (28%)
		FN3	536..623	CDD:238020	24/99 (24%)
		EphA2_TM	644..736	CDD:291255	26/92 (28%)
		PTKc_EphR	736..1002	CDD:270629	171/266 (64%)
		Pkinase_Tyr	741..999	CDD:285015	167/258 (65%)
		SAM_1	1033..1092	CDD:278937	19/55 (35%)
		SAM	1039..1092	CDD:197735	17/49 (35%)
epha2b	XP_005162266.1	Ephrin_lbd	28..195	CDD:279712	63/181 (35%)
		FN3	321..415	CDD:238020	31/107 (29%)
		FN3	432..531	CDD:238020	28/127 (22%)
		EphA2_TM	539..611	CDD:291255	29/98 (30%)
		PTKc_EphR_A2	610..877	CDD:133194	172/268 (64%)
		Pkinase_Tyr	616..873	CDD:285015	167/258 (65%)
		SAM_superfamily	904..973	CDD:301707	21/63 (33%)
		SAM	904..970	CDD:197735	21/63 (33%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	396	1.000	Domainoid score	I723
eggNOG	1	0.900	-	-		E2759_KOG0196
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	753	1.000	Inparanoid score	I540
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	1	1.000	-	-		FOG0000086
OrthoInspector	1	1.000	-	-		otm24639
orthoMCL	1	0.900	-	-		OOG6_100189
Panther	1	1.100	-	-	O	PTHR24416
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X82
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	11	10.870