Protein Alignment Eph and ephb2a

DIOPT Version :10

• Sequence 1: NP_524625.3
• Gene: Eph / 43803
• FlyBaseID: FBgn0025936
• Length: 1096
• Species: Drosophila melanogaster
• Sequence 2: XP_021325624.1
• Gene: ephb2a / 562176
• ZFINID: ZDB-GENE-070713-2
• Length: 999
• Species: Danio rerio
• Alignment Length: 1063
• Identity: 455/1063 - (42%)
• Similarity: 626/1063 - (58%)
• Gaps: 124/1063 - (11%)

  Fly    62 MSLLRTILLIIISIHFKLAHADQVVLLD-TTREATLEWTRYPYGPQAQTPGWVEESFTDFVKGIN 125
            |.::|.:.:|      :|..|.:.||:| ||..|.|.||.||      :.||.|.|..|  :.:|
Zfish    17 MDIIRLLWII------RLVWAVEDVLMDSTTATAELGWTIYP------SLGWEEVSGYD--ENMN 67

  Fly   126 W-RSYVVCDVAYHNVNNWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFD 189
            . |:|.||:|...|.|||:.:.:|.|..|.|:::|::|::||||..|....||||||:|.::|.|
Zfish    68 TIRTYQVCNVFDANQNNWVRTKYIRRRGAQRIHVEMKFSVRDCSSIPKVPGSCKETFNLYYFESD 132

  Fly   190 A--ATREPPPWQTDSYRLIARIAAGEGRFNQNSDVD-------INTEVKSIA-VNKKGVYFAFRD 244
            :  ||:..|.|..:.:..:..|||.|. |:|   ||       |||||:|.. |::.|.|.||:|
Zfish   133 SDTATKVYPAWMENPWVKVDTIAADES-FSQ---VDLGGRVMKINTEVRSFGPVSRSGFYLAFQD 193

  Fly   245 QGACISVLAVKVYYITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYL-CKGDG 308
            .|||:|::||||:|..||.:..|.|.|.||.:|.|.|.:....||||.|.|..:.|..| |.|||
Zfish   194 YGACMSLIAVKVFYRKCPRIIRNGAIFQETLSGAESTSLVSARGTCVPNGEEVDVPIKLYCNGDG 258

  Fly   309 KWTILTGGCRCKAGYEPNYTNKTCTECPLGTFKSPE-VTKCTPCPPNSNASKTGSPFCKCASGFY 372
            :|.:..|.|.||||:|.......|..||.|:||:.: ..:|..||.||..|..|:..|.|.:|:|
Zfish   259 EWLVPIGRCVCKAGHESVDNGTMCKACPFGSFKASQGDQQCLQCPINSRTSSEGATSCVCRNGYY 323

  Fly   373 RHPNDGRHMPCYSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKI---- 433
            |..:|...|||.:.|:||..: :..|::||..:.|..|  .||...|        |:||.|
Zfish   324 RTDSDPLQMPCTTVPSAPQRV-ISSVNETSLRLEWDVP--KESGGRE--------DVVYNIICKS 377

  Fly   434 ------KCNICSPNVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSL 492
                  .|..|..||.:.|......|.:|.:::|...|.||.:|.|:|.||..:.:     ....
Zfish   378 CGSGRGACTRCGDNVQFVPRQLGLTEPRIHISDLLAHTQYTFEIQAVNGVSDQSPY-----SPQF 437

  Fly   493 VAVSDIVFSNTSLLNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQS 557
            .||:                  :.|.||    ...:|.:....:..:.:||     |.|.:|.|.
Zfish   438 TAVN------------------ITTNQA----APSAVSIMHQVSRAVDSIT-----LSWSQPDQP 475

  Fly   558 DFPLEFYEVRWFPKVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQ 622
            :..:..||::::.|..::: |.|.|.::...|.|.||...|.|.||||.:|..|||.:|..:|.|
Zfish   476 NGVILDYELQYYEKNAVES-NSSLLRSQTNTAVIRGLKSGTIYVFQVRARTVAGFGRFSGKMYFQ 539

  Fly   623 TLQSVGSVYDDSVQ--IRFIAGAIVTGVLFLVIFIIATVYFMRSKHQDDLDKKSTNHLPLPLDYA 685
            |:..  ..|:.|:|  :..|.|:...||:||:..::..:...| :..|..|.:.|:.|.   .|.
Zfish   540 TMTE--EEYNSSLQEKLPLIIGSAAAGVVFLIAVVVLIIVCNR-RGSDRTDSEYTDKLQ---HYT 598

  Fly   686 SNEVNSMDTTPIVKKLHLNVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGG 750
            |..::.                    ..:.|:||.||||||:|:||||:|||...:.||.:||.|
Zfish   599 SGHMSP--------------------GMKIYIDPFTYEDPNEAVREFAKEIDVTCVKIEQVIGAG 643

  Fly   751 EFGDVCRGRLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSN 815
            |||:||.|.|::|..  ::|.|||||||.|.:||.|.|||:||||||||||||:|:|:||||:|.
Zfish   644 EFGEVCSGNLRLPGK--REILVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNIIHLEGVVTKST 706

  Fly   816 PVMIITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQL 880
            |||||||:|||||||:|||.|||:|..:||:.||||||:||.||.|||||||||||||:|||:.|
Zfish   707 PVMIITEFMENGSLDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLCDMNYVHRDLAARNILVNSNL 771

  Fly   881 ICKIADFGLSREIE-NASD-AYTTR-GGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYG 942
            :||::||||||.:| :.|| .||:. |||||:||||||||.:|||||:|||||||:|:|||||||
Zfish   772 VCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSSSDVWSYGIVMWEVMSYG 836

  Fly   943 ERPYWNWSNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQ 1007
            |||||:.||||||.:||:.||||.|||||.||:|||||||||.|.:||.|:.||:|||.:.|.|.
Zfish   837 ERPYWDMSNQDVINAIEQDYRLPPPMDCPSALHQLMLDCWQKDRNNRPKFSQIVNTLDKMIRNPG 901

  Fly  1008 SLLTTRPSPESDGNH--ILDGQRGQ-NIFISTDLWLEHIKMSRYCHHFKEANLINAQQISRLTAQ 1069
            ||..|  :|.|.|.|  :||..... :.|.:.|.||:.||||:|..:|...:|...:.:|::|..
Zfish   902 SLKAT--TPLSSGVHLPLLDRSTPDFSSFSTVDEWLDAIKMSQYKENFANEDLTTFEAVSQMTMD 964

  Fly  1070 QLSDMGITLVGHQKKILHQARQL 1092
            .:..:|:||.|||||||:..:.:
Zfish   965 DILRVGVTLAGHQKKILNSVQMM 987

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	79/186 (42%)
		FN3	387..476	CDD:238020	28/98 (29%)
		FN3	536..623	CDD:238020	28/86 (33%)
		EphA2_TM	640..738	CDD:464211	29/97 (30%)
		PTKc_EphR	736..1002	CDD:270629	187/268 (70%)
		SAM_1	1029..1092	CDD:425739	23/63 (37%)
ephb2a	XP_021325624.1	EphR_LBD	32..209	CDD:470656	79/188 (42%)
		fn3	339..434	CDD:394996	30/110 (27%)
		fn3	450..533	CDD:394996	27/88 (31%)
		EphA2_TM	557..631	CDD:464211	29/97 (30%)
		PTKc_EphR_B	629..897	CDD:173638	187/269 (70%)
		SAM_EPH-B2	923..993	CDD:188951	23/65 (35%)