DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Eph and ephb2b

DIOPT Version :9

Sequence 1:	NP_524625.3	Gene:	Eph / 43803	FlyBaseID:	FBgn0025936	Length:	1096	Species:	Drosophila melanogaster
Sequence 2:	NP_001314672.1	Gene:	ephb2b / 554232	ZFINID:	ZDB-GENE-050522-415	Length:	989	Species:	Danio rerio

Alignment Length:	1063	Identity:	446/1063 - (41%)
Similarity:	620/1063 - (58%)	Gaps:	127/1063 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly    68 ILLIIISIHFKLAHADQVVLLDTTREATLEWTRYPYGPQAQTPGWVEESFTDFVKGINW-RSYVV 131
            ||.|:.::...|| .:::::..||..|.|.||.||....:.: .|.|.|..|  :.:|. |:|.|
Zfish     4 ILWILWTLPSVLA-IEEILMDSTTATAELGWTVYPVSGTSDS-SWEEVSGYD--ENMNTIRTYQV 64

  Fly   132 CDVAYHNVNNWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDA--ATRE 194
            |:|...|.|||:.:.:|.|..|.|:::|::|::||||..|....||||||:|.:||.|:  ||:.
Zfish    65 CNVFDANQNNWVRTKYIRRRGAQRIHVEMKFSVRDCSSIPRVPGSCKETFNLYYYESDSDTATKT 129

  Fly   195 PPPWQTDSYRLIARIAAGEGRFNQNSDVD-------INTEVKSIA-VNKKGVYFAFRDQGACISV 251
            .|||..:.:..:..|||.|. |:|   ||       |||||:|.. |::.|.|.||:|.|.|:|:
Zfish   130 FPPWMENPWIKVDTIAADES-FSQ---VDLGGRVMKINTEVRSFGPVSRNGFYLAFQDYGGCMSL 190

  Fly   252 LAVKVYYITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYL-CKGDGKWTILTG 315
            :||:|:|..||....|.|.|.||.:|.|.|.:....|.|:.|||..:.|..| |.|||:|.:..|
Zfish   191 IAVRVFYRKCPRTIRNGAIFQETLSGAESTSLVAARGVCIPNAEEVDVPIKLYCNGDGEWMVPIG 255

  Fly   316 GCRCKAGYEPNYTNKTCTECPLGTFKSPE-VTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGR 379
            .|.||||:|.......|..||.|.||:.: ..||..||.||..:..|:..|.|.:|:||..:|..
Zfish   256 RCMCKAGHEAVENGTVCRACPSGFFKTAQGDEKCLQCPINSRTTNDGATNCVCRNGYYRTDSDPL 320

  Fly   380 HMPCYSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKI----------K 434
            .|||.:.|:||.|: :..|::||.::.|:.|       .||..:   .|:||.|          .
Zfish   321 EMPCTTVPSAPQNV-ISSVNETSLMLEWNPP-------RETGGR---DDVVYNIICKSCGGGRGG 374

  Fly   435 CNICSPNVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIV 499
            |..|..||.:.|......|:::.:::|...|.||.:|.|:|.||
Zfish   375 CTRCGDNVQFIPRQLGLTESRVFISDLLAHTQYTFEIQAVNGVS--------------------- 418

  Fly   500 FSNTSLLNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFY 564
                       |.:........:..||.....|||..:..::.|.....|.|.:|.|.:..:..|
Zfish   419 -----------DQSPYSPQYTSVNITTNQAAPSTVSIMHQVSRTIDSITLSWSQPDQPNGVILDY 472

  Fly   565 EVRWFPKVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGS 629
            |::::.|.:.:. |.|.:.::...|.|.||.....|.||||.:|..|||.||..:|.||:..  .
Zfish   473 ELQYYEKDQTEH-NSSMVKSQTNTAVIRGLKPGGIYVFQVRARTVAGFGRYSGKMYFQTMTE--E 534

  Fly   630 VYDDSVQ--IRFIAGAIVTGVLFLVIFIIATVYFMR-------SKHQDDLDKKSTNHLPLPLDYA 685
            .|:.|:|  :..|.|:...|::||:..::..:...|       |::.|.|...::.|:
Zfish   535 EYNTSIQEKLPLIIGSAAAGLVFLIAVVVIIIVCNRRGFERADSEYTDKLQHYTSGHM------- 592

  Fly   686 SNEVNSMDTTPIVKKLHLNVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGG 750
                     :|.:|               .|:||.||||||:|:||||:|||.:.:.||.:||.|
Zfish   593 ---------SPGMK---------------IYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAG 633

  Fly   751 EFGDVCRGRLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSN 815
            |||:||.|.||:|..  :::.|||||||.|.:||.|.|||:||||||||||||||:|:||||:|:
Zfish   634 EFGEVCSGNLKLPGK--REMFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSS 696

  Fly   816 PVMIITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQL 880
            |||||||:|||||||:|||.|||:|..:||:.|||||||||.||:|||||||||||||:|||:.|
Zfish   697 PVMIITEFMENGSLDSFLRQNDGQFTVIQLVGMLRGIASGMKYLADMNYVHRDLAARNILVNSNL 761

  Fly   881 ICKIADFGLSREIE-NASD-AYTTR-GGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYG 942
            :||::||||||.:| :.|| .||:. |||||:||||||||.:|||||||||||||:|:|||||||
Zfish   762 VCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYG 826

  Fly   943 ERPYWNWSNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQ 1007
            |||||:.:|||||.:||:.||||.|||||.||:|||||||||.|.:||.|:.||:.||.:.|.|.
Zfish   827 ERPYWDMTNQDVINAIEQDYRLPPPMDCPSALHQLMLDCWQKDRNNRPKFSQIVNNLDKMIRNPN 891

  Fly  1008 SLLTTRPSPESDGNH--ILDGQRGQ-NIFISTDLWLEHIKMSRYCHHFKEANLINAQQISRLTAQ 1069
            ||...  :|.|.|.|  :||..... :.|.|.|.||:.|||.:|..:|...|..:...:|::|.:
Zfish   892 SLKAM--TPLSSGVHLPLLDRSVPDFSSFSSVDDWLDAIKMGQYKDNFANGNFTSFDLVSQMTME 954

  Fly  1070 QLSDMGITLVGHQKKILHQARQL 1092
            .:..:|:||.|||||||:..:.:
Zfish   955 DILRVGVTLAGHQKKILNSVQMM 977

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	75/185 (41%)
		FN3	387..476	CDD:238020	27/98 (28%)
		FN3	536..623	CDD:238020	26/86 (30%)
		EphA2_TM	644..736	CDD:291255	24/98 (24%)
		PTKc_EphR	736..1002	CDD:270629	188/268 (70%)
		Pkinase_Tyr	741..999	CDD:285015	184/260 (71%)
		SAM_1	1033..1092	CDD:278937	23/58 (40%)
		SAM	1039..1092	CDD:197735	20/52 (38%)
ephb2b	NP_001314672.1	None

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	396	1.000	Domainoid score	I723
eggNOG	1	0.900	-	-		E2759_KOG0196
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	753	1.000	Inparanoid score	I540
OMA	1	1.010	-	-		QHG45908
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	1	1.000	-	-		FOG0000086
OrthoInspector	1	1.000	-	-		otm24639
orthoMCL	1	0.900	-	-		OOG6_100189
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2895
SonicParanoid	1	1.000	-	-		X82
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	13	12.770