DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Eph and Epha4

DIOPT Version :9

Sequence 1:	NP_524625.3	Gene:	Eph / 43803	FlyBaseID:	FBgn0025936	Length:	1096	Species:	Drosophila melanogaster
Sequence 2:	NP_001155883.1	Gene:	Epha4 / 316539	RGDID:	1560587	Length:	986	Species:	Rattus norvegicus

Alignment Length:	1053	Identity:	441/1053 - (41%)
Similarity:	600/1053 - (56%)	Gaps:	146/1053 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly    82 ADQVVLLDT-TREATLEWTRYPYGPQAQTPGWVEESFTDFVKGINWRSYVVCDVAYHNVNNWLWS 145
            |::|.|||: :.:..|.|...|.     ..||.|.|..| .|....|:|.||:|...:.||||.:
  Rat    28 ANEVTLLDSRSVQGELGWIASPL-----EGGWEEVSIMD-EKNTPIRTYQVCNVMEASQNNWLRT 86

  Fly   146 PFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDAATREPPPWQTDSYRLIAR-- 208
            .:|.|..|.|:||||:||:|||:..||...:|||||:|.:||.|          .|..|.|..
  Rat    87 DWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESD----------NDKERFIRESQ 141

  Fly   209 ------IAAGEGRFNQNSDVDI-------NTEVKSIA-VNKKGVYFAFRDQGACISVLAVKVYYI 259
                  |||.|. |.|   |||       |||::.:. ::|||.|.||:|.||||::::|:|:|.
  Rat   142 FGKIDTIAADES-FTQ---VDIGDRIMKLNTEIRDVGPLSKKGFYLAFQDVGACIALVSVRVFYK 202

  Fly   260 TCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYLCKGDGKWTILTGGCRCKAGYE 324
            .||....|.|.|.:|.||.:.:.:.:..|:||:|:|..:.|...|..||:|.:..|.|.|.||:|
  Rat   203 KCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGHE 267

  Fly   325 PNYTNKTCTECPLGTFK--SPEVTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPCYSPP 387
            ..  |..|..|.:|.:|  |.:.| |..|||:|.:...|:..|.|..||:|..||...|||..||
  Rat   268 EQ--NGECQACKIGYYKALSTDAT-CAKCPPHSYSVWEGATSCTCDRGFFRADNDAASMPCTRPP 329

  Fly   388 AAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKI-----------KCNICSPN 441
            :||.|| :..|::||..:.||:|       ..|..:   .||.|.:           ||..|...
  Rat   330 SAPLNL-ISNVNETSVNLEWSSP-------QNTGGR---QDISYNVVCKKCGAGDPSKCRPCGSG 383

  Fly   442 VVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLL 506
            |.|.|..:....|::::|:|...|.||.:|.|:|.||..|.    |.:.|:
  Rat   384 VHYTPQQNGLKTTRVSITDLLAHTNYTFEIWAVNGVSKYNP----SPDQSV-------------- 430

  Fly   507 NIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRWFPK 571
                          .:..||.....|::..::...:|.....|.|.:|.:.:..:..|||:::.|
  Rat   431 --------------SVTVTTNQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEK 481

  Fly   572 VELD----AINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQT----LQSVG 628
            .:.:    .:..:|.||     .|.||...|.|.|.||.:|..|:|.:|..:...|    .:.:|
  Rat   482 DQNERSYRIVRTAARNT-----DIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIG 541

  Fly   629 SVYDDSVQIRFIAGAIVTGVLFLVIFIIATVYFMRSKHQDDLDKKSTNHLPLPLDYASNEVNSMD 693
            ...:.:|.:..::|::|..|:.:..|:|:.   .|||:..                |..|.:
  Rat   542 DGANSTVLLVSVSGSVVLVVILIAAFVISR---RRSKYSQ----------------AKQEAD--- 584

  Fly   694 TTPIVKKLHLNVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRG 758
                 ::.|||      ...|:||||.||||||||:||||:||||:.|.||.:||.||||:||.|
  Rat   585 -----EEKHLN------QGVRTYVDPFTYEDPNQAVREFAKEIDASCIKIEKVIGVGEFGEVCSG 638

  Fly   759 RLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITEY 823
            |||:|..  ::|.|||||||.|.::|.|.|||:||||||||||||:|:|:||||:..||||||||
  Rat   639 RLKVPGK--REICVAIKTLKAGYTDKQRRDFLSEASIMGQFDHPNIIHLEGVVTKCKPVMIITEY 701

  Fly   824 MENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFG 888
            |||||||.|||.|||:|..:||:.|||||.|||.|||||:||||||||||:|||:.|:||::|||
  Rat   702 MENGSLDAFLRKNDGRFTVIQLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSNLVCKVSDFG 766

  Fly   889 LSREIENASD-AYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWSNQ 952
            :||.:|:..: ||||||||||:|||||||||:|||||||||||||:|:||||||||||||:.|||
  Rat   767 MSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQ 831

  Fly   953 DVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTTRPSPE 1017
            ||||:||:|||||.|||||.||:|||||||||:|:.||.|..||:.||.|.|.|.||..|.|...
  Rat   832 DVIKAIEEGYRLPPPMDCPIALHQLMLDCWQKERSDRPKFGQIVNMLDKLIRNPNSLKRTGPESS 896

  Fly  1018 SDGNHILDGQRGQ-NIFISTDLWLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDMGITLVGH 1081
            .....:||....: :..:|...||:.|||.||..:|..|.....:.:..::...|:.:|||.:.|
  Rat   897 RPNTALLDPSSPEFSAVVSVGDWLQAIKMDRYKDNFTAAGYTTLEAVVHMSQDDLARIGITAITH 961

  Fly  1082 QKKILHQARQLDT 1094
            |.|||...:.:.|
  Rat   962 QNKILSSVQAMRT 974

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	75/191 (39%)
		FN3	387..476	CDD:238020	29/99 (29%)
		FN3	536..623	CDD:238020	23/90 (26%)
		EphA2_TM	644..736	CDD:291255	29/91 (32%)
		PTKc_EphR	736..1002	CDD:270629	193/266 (73%)
		Pkinase_Tyr	741..999	CDD:285015	188/258 (73%)
		SAM_1	1033..1092	CDD:278937	19/58 (33%)
		SAM	1039..1092	CDD:197735	18/52 (35%)
Epha4	NP_001155883.1	EphR_LBD_A4	30..203	CDD:198450	75/192 (39%)
		FN3	329..436	CDD:238020	35/149 (23%)
		FN3	441..532	CDD:238020	24/95 (25%)
		EphA2_TM	562..618	CDD:405290	29/88 (33%)
		PTKc_EphR_A	616..882	CDD:270651	193/267 (72%)
		SAM_EPH-A4	911..981	CDD:188944	20/64 (31%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	399	1.000	Domainoid score	I718
eggNOG	1	0.900	-	-		E2759_KOG0196
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	763	1.000	Inparanoid score	I521
OMA	1	1.010	-	-		QHG45908
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	1	1.000	-	-		FOG0000086
OrthoInspector	1	1.000	-	-		otm46382
orthoMCL	1	0.900	-	-		OOG6_100189
Panther	1	1.100	-	-	LDO	PTHR24416
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X82
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	13	12.880