DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Eph and Ephb2

DIOPT Version :9

Sequence 1:	NP_524625.3	Gene:	Eph / 43803	FlyBaseID:	FBgn0025936	Length:	1096	Species:	Drosophila melanogaster
Sequence 2:	XP_038965983.1	Gene:	Ephb2 / 313633	RGDID:	1564232	Length:	988	Species:	Rattus norvegicus

Alignment Length:	1057	Identity:	446/1057 - (42%)
Similarity:	609/1057 - (57%)	Gaps:	126/1057 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly    79 LAHADQVVLLDTTREATLEWTRYPYGPQAQTPGWVEESFTDFVKGINW-RSYVVCDVAYHNVNNW 142
            ||..::.::..||..|.|.|..:|      ..||.|.|..|  :.:|. |:|.||:|...:.|||
  Rat    16 LAAVEETLMDSTTATAELGWMVHP------PSGWEEVSGYD--ENMNTIRTYQVCNVFESSQNNW 72

  Fly   143 LWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYE--FDAATREPPPWQTDSYRL 205
            |.:.||.|..|:|:::|::|::||||..|....||||||:|.:||  ||.||:..|.|..:.:..
  Rat    73 LRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADFDLATKTFPNWMENPWVK 137

  Fly   206 IARIAAGEGRFNQNSDVD-------INTEVKSIA-VNKKGVYFAFRDQGACISVLAVKVYYITCP 262
            :..|||.|. |:|   ||       |||||:|.. |::.|.|.||:|.|.|:|::||:|:|..||
  Rat   138 VDTIAADES-FSQ---VDLGGRVMKINTEVRSFGPVSRNGFYLAFQDYGGCMSLIAVRVFYRKCP 198

  Fly   263 AVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYL-CKGDGKWTILTGGCRCKAGYEPN 326
            .|.:|.|.|.||.:|.|.|.:....|:|:.|||..:.|..| |.|||:|.:..|.|.||||:|..
  Rat   199 RVIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAV 263

  Fly   327 YTNKTCTECPLGTFKSPEVTK-CTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPCYSPPAAP 390
            .....|..||.||||:.:..: ||.||.||..:..|:..|.|.:|:||...|...|||.:.|:||
  Rat   264 ENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAP 328

  Fly   391 TNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKI----------KCNICSPNVVYN 445
             ...:..|::||.::.|:.|  .:|...|        |:||.|          .|..|..||.|.
  Rat   329 -QAVISSVNETSLMLEWTPP--RDSGGRE--------DLVYNIICKSCGSGRGACTRCGDNVQYA 382

  Fly   446 PSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLLNIPL 510
            |......|.:|.:::|...|.||.:|.|:|.|:..:.|....
  Rat   383 PRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQF----------------------- 424

  Fly   511 DLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRWFPKVELD 575
                     |.:..||.....|.|..:..::.|.....|.|.:|.|.:..:..||::::.|.||.
  Rat   425 ---------ASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKQELS 480

  Fly   576 AINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGSVYDDSV--QIR 638
            ..|.:|:.:......:.||.....|.||||.:|..|:|.||..:|.||:..  :.|..|:  ::.
  Rat   481 EYNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTE--AEYQTSIKEKLP 543

  Fly   639 FIAGAIVTGVLFLVIFIIATVYFMRSKHQDDLDKKSTNHLPLPLDYASNEVNSMDTTPIVKKLHL 703
            .|.|:...||:|::..::..:...|.:..:..|.:.|:.|.   .|.|..:     ||.:|
  Rat   544 LIVGSSAAGVVFVIAVVVIAIVCNRRRGFERADSEYTDKLQ---HYTSGHM-----TPGMK---- 596

  Fly   704 NVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRGRLKIPPNFVQ 768
                       .|:||.||||||:|:||||:|||.:.:.||.:||.||||:||.|.||:|..  :
  Rat   597 -----------IYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAGEFGEVCSGHLKLPGK--R 648

  Fly   769 DIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITEYMENGSLDTFL 833
            :|.|||||||.|.:||.|.|||:||||||||||||||:|:||||:|.|||||||:|||||||:||
  Rat   649 EIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIITEFMENGSLDSFL 713

  Fly   834 RVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFGLSREIE-NAS 897
            |.|||:|..:||:.||||||:||.||:|||||||||||||:|||:.|:||::||||||.:| :.|
  Rat   714 RQNDGQFTVIQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTS 778

  Fly   898 D-AYTTR-GGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWSNQDVIKSIEK 960
            | .||:. |||||:||||||||.:|||||||||||||:|:||||||||||||:.:|||||.:||:
  Rat   779 DPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAIEQ 843

  Fly   961 GYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTTRPSPESDGNHILD 1025
            .||||.|||||.||:|||||||||.|.|||.|..||:|||.:.|.|.||....|........:||
  Rat   844 DYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGINLPLLD 908

  Fly  1026 GQRGQNI-----FISTDLWLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDMGITLVGHQKKI 1085
                :.|     |.:.|.|||.|||.:|...|..|...:...:|::..:.:..:|:||.||||||
  Rat   909 ----RTIPDYTSFNTVDEWLEAIKMGQYKESFTNAGFTSFDVVSQMMMEDILRVGVTLAGHQKKI 969

  Fly  1086 LH-------QARQLDTI 1095
            |:       |..|:.::
  Rat   970 LNSIQVMRAQMNQIQSV 986

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	75/185 (41%)
		FN3	387..476	CDD:238020	28/98 (29%)
		FN3	536..623	CDD:238020	25/86 (29%)
		EphA2_TM	644..736	CDD:291255	26/91 (29%)
		PTKc_EphR	736..1002	CDD:270629	190/268 (71%)
		Pkinase_Tyr	741..999	CDD:285015	185/260 (71%)
		SAM_1	1033..1092	CDD:278937	23/65 (35%)
		SAM	1039..1092	CDD:197735	21/59 (36%)
Ephb2	XP_038965983.1	EphR_LBD_B2	19..196	CDD:198445	75/188 (40%)
		fn3	326..421	CDD:394996	29/105 (28%)
		fn3	437..521	CDD:394996	24/83 (29%)
		EphA2_TM	<566..619	CDD:405290	24/75 (32%)
		PTKc_EphR_B	618..886	CDD:173638	190/269 (71%)
		SAM_EPH-B2	912..982	CDD:188951	23/69 (33%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	399	1.000	Domainoid score	I718
eggNOG	1	0.900	-	-		E2759_KOG0196
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	763	1.000	Inparanoid score	I521
OMA	1	1.010	-	-		QHG45908
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	1	1.000	-	-		FOG0000086
OrthoInspector	1	1.000	-	-		otm46382
orthoMCL	1	0.900	-	-		OOG6_100189
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X82
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	12	11.740