Protein Alignment Eph and ephb4a

DIOPT Version :10

• Sequence 1: NP_524625.3
• Gene: Eph / 43803
• FlyBaseID: FBgn0025936
• Length: 1096
• Species: Drosophila melanogaster
• Sequence 2: NP_571489.1
• Gene: ephb4a / 30688
• ZFINID: ZDB-GENE-990415-62
• Length: 987
• Species: Danio rerio
• Alignment Length: 1075
• Identity: 423/1075 - (39%)
• Similarity: 593/1075 - (55%)
• Gaps: 166/1075 - (15%)

  Fly    72 IISIHFKLAH-ADQVVLLDT-TREATLEWTRYPYGPQAQTPGWVEESFTDFVKGINWRSYVVCDV 134
            :|.:.|.|.. |::.||::| |..:.|:||.:    ....|.|.|.|..| .:..:.|:|.:|.
Zfish    13 VILLEFLLGSVAEEEVLMNTKTETSDLKWTTH----SRSKPEWEEVSGLD-EENNSVRTYQICQ- 71

  Fly   135 AYHNVNNWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDA--ATREPPP 197
            |..:.::.:.|..|:|..|:::|:|:.||:.:||....:..||||||:|.:|:.|.  ||...|.
Zfish    72 ADGSSSHCVRSKLIERRGASQVYVELFFTMVECSSRNTHHRSCKETFNLYYYQSDTDDATATHPA 136

  Fly   198 WQTDSYRLIARIAA-------GEGRFNQNSDVDINTEVKSI---AVNKKGVYFAFRDQGACISVL 252
            |..:.|..:..:||       ||.:.|          ||::   .::|:|.|.||:.||||:::|
Zfish   137 WMENPYTKVDTVAADFLLRKGGEKKVN----------VKTLRLGPLSKRGFYLAFQAQGACMALL 191

  Fly   253 AVKVYYITCPAVTENFAHFNET-PTGREITIIEKQNGTCVDN-AEPYET---PTYLCKGDGKWTI 312
            :|:|::..|||:|.:.:.|.|| |.    :::::..|.||.| |:|..:   |...|..||:|..
Zfish   192 SVRVFFKKCPALTRSLSVFPETVPR----SLVQEAVGQCVANAAQPGPSPRPPKMFCGEDGQWVD 252

  Fly   313 L-TGGCRCKAGYEPNYTNKTCTECPLGTFKSPEVT-KCTPCPPNSNASKTGSPFCKCASGFYRHP 375
            . |..|.|..|:|.::....|..||:|.||....| .|:.||.||...:|||..|.|.|||||..
Zfish   253 QPTTTCTCLPGFEASHGELECRACPVGLFKMGSGTGPCSVCPENSQTGETGSAACVCRSGFYRAL 317

  Fly   376 NDGRHMPCYSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKIK------ 434
            :|....||..||::|.: .:..|:.||..:.||.|..:..          .||:.|.::
Zfish   318 SDSADTPCTRPPSSPRS-PVPQVNDTSLTLEWSEPLDSGG----------RSDLSYSVECRMCST 371

  Fly   435 ----CNICSPNVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAV 495
                |.:||..|.|.||.......::::..|.|.|||:..:.|:|.||      ..|.:......
Zfish   372 PGSPCTLCSDGVNYRPSQTGIQGRRVSIWGLRPHTTYSFTVMALNGVS------AQSQQGPAGET 430

  Fly   496 SDIVFSNTSLLNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFP 560
            .:|    |:..|:|                   ||:|   .||....|.....|.|:.|.||.:.
Zfish   431 INI----TTSPNVP-------------------VLVS---GLRKSTATESSLTLYWNTPTQSHYR 469

  Fly   561 LEFYEVRWFPKVELDAINKSA--LNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQT 623
            :..|::|:..| |..:...|.  :.:...:..:..|...|:|..|||.:|..|:||:...|..:|
Zfish   470 ILQYQIRYCEK-ERGSEENSCHYMESNNNEVVLSDLRRATQYEVQVRARTFAGYGSFGKAILFRT 533

  Fly   624 LQSVGSVYDDSVQIRFIAGAIVT-GVLFLVIFIIATVYFMRSKH---QDDLDKKSTNHLPLPLDY 684
            |..    .|||.....:.|.::. |:|.|:|.|.|.:|.:|.::   ..:|..|:..:
Zfish   534 LPD----EDDSSSPLLVTGILIAMGMLLLIIVIGAAIYCIRKQNNYKDPELSDKNGQY------- 587

  Fly   685 ASNEVNSMDTTPIVKKLHLNVTTPLFGNS-RSYVDPHTYEDPNQAIREFAREIDANYITIEAIIG 748
                                    |.|.. :.|:||.||||||:|:||||:|||.:.:.||.:||
Zfish   588 ------------------------LMGQGVKVYIDPFTYEDPNEAVREFAKEIDVSCVKIEEVIG 628

  Fly   749 GGEFGDVCRGRLKIP---PNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGV 810
            .||||:||||||:||   .|:     |||||||.|.::|.|.|||.||||||||.|||:|:|:|:
Zfish   629 AGEFGEVCRGRLRIPGKKENY-----VAIKTLKGGYTDKQRRDFLAEASIMGQFQHPNIIHLEGI 688

  Fly   811 VTRSNPVMIITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVL 875
            :|.|.||||:||:||||:||:|||:|||:|..:||:.|||||||||.|||:|:||||||||||:|
Zfish   689 ITASCPVMILTEFMENGALDSFLRLNDGQFTPIQLVGMLRGIASGMKYLSEMSYVHRDLAARNIL 753

  Fly   876 VNAQLICKIADFGLSREI-ENASD-AYTTR-GGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWE 937
            ||:.|:||::||||||.: ||:|| .||:. |||||:||||||||||||||.||||||||:|:||
Zfish   754 VNSNLVCKVSDFGLSRFLQENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTCASDVWSYGIVMWE 818

  Fly   938 VMSYGERPYWNWSNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNL 1002
            |||:||||||:.||||||.:||:.||||.|.||...|:|||||||||:||.||.||:|||.||.|
Zfish   819 VMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCSTYLHQLMLDCWQKERTARPRFANIVSALDKL 883

  Fly  1003 ARQPQSLLTTRPSPESDGNHILD----------GQRGQNIFISTDLWLEHIKMSRYCHHFKEANL 1057
            .|.|.||..|........:.:||          |..|.        ||..|||.||...|.:|..
Zfish   884 IRNPASLKITAQEGAGPSHPLLDQRSPLTPSSCGTVGD--------WLRAIKMERYEETFLQAGY 940

  Fly  1058 INAQQISRLTAQQLSDMGITLVGHQKKILHQARQL 1092
            .:.|.::.:..:.|..:||||.|||||||.....|
Zfish   941 TSMQLVTHINTEDLLRLGITLAGHQKKILSSIEAL 975

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	59/187 (32%)
		FN3	387..476	CDD:238020	24/98 (24%)
		FN3	536..623	CDD:238020	24/88 (27%)
		EphA2_TM	640..738	CDD:464211	29/102 (28%)
		PTKc_EphR	736..1002	CDD:270629	185/271 (68%)
		SAM_1	1029..1092	CDD:425739	23/62 (37%)
ephb4a	NP_571489.1	EphR_LBD	26..199	CDD:470656	59/188 (31%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	319..340		6/21 (29%)
		fn3	339..418	CDD:394996	23/88 (26%)
		FN3	440..533	CDD:238020	28/115 (24%)
		EphA2_TM	547..618	CDD:464211	29/101 (29%)
		PTKc_EphR_B	616..884	CDD:173638	185/272 (68%)
		SAM_EPH-B4	914..980	CDD:188953	25/70 (36%)