DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Eph and Epha3

DIOPT Version :9

Sequence 1:	NP_524625.3	Gene:	Eph / 43803	FlyBaseID:	FBgn0025936	Length:	1096	Species:	Drosophila melanogaster
Sequence 2:	NP_113752.2	Gene:	Epha3 / 29210	RGDID:	68389	Length:	984	Species:	Rattus norvegicus

Alignment Length:	1071	Identity:	429/1071 - (40%)
Similarity:	608/1071 - (56%)	Gaps:	138/1071 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly    62 MSLLRTILLIIISIHFKLA--HADQVVLLDT-TREATLEWTRYPYGPQAQTPGW-----VEESFT 118
            :|:|..:...::|...:|:  .:::|.|||: |.:..|.|..||      :.||     |:|.:|
  Rat     5 LSILILLGCCVLSCSRELSPQPSNEVNLLDSKTIQGELGWISYP------SHGWEEISGVDEHYT 63

  Fly   119 DFVKGINWRSYVVCDVAYHNVNNWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSL 183
            ..      |:|.||:|..|:.||||.:.::.|.||.::|:|::||:|||:..|....:|||||:|
  Rat    64 PI------RTYQVCNVMDHSQNNWLRTNWVPRNSAQKIYVELKFTLRDCNSIPLVLGTCKETFNL 122

  Fly   184 LFYEFDAATREPPPWQTDSYRLIARIAAGEGRFNQ----NSDVDINTEVKSIA-VNKKGVYFAFR 243
            .:.|.|  ......:....:..|..|||.|. |.|    :..:.:|||::.:. |||||.|.||:
  Rat   123 YYMESD--DDHGVKFLEHQFTKIDTIAADES-FTQMDLGDRILKLNTEIREVGPVNKKGFYLAFQ 184

  Fly   244 DQGACISVLAVKVYYITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYLCKGDG 308
            |.|||:::::|:||:..||...:|.|.|.:|......:::|.: |:||:|::..:.|...|..:|
  Rat   185 DVGACVALVSVRVYFKKCPFTVKNLAMFPDTVPMDSQSLVEVR-GSCVNNSKEEDPPRMYCSTEG 248

  Fly   309 KWTILTGGCRCKAGYEPNYTNKTCTECPLGTFKSPE-VTKCTPCPPNSNASKTGSPFCKCASGFY 372
            :|.:..|.|.|.||||..  ...|..|..|.:|:.: |.|||.|||:|:..:.||..|:|.:.::
  Rat   249 EWLVPIGKCTCNAGYEER--GFICQACRPGFYKALDGVAKCTKCPPHSSTQEDGSMNCRCENNYF 311

  Fly   373 RHPNDGRHMPCYSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKI---- 433
            |...|...|.|..||:||.|: :..:::||.|:.||.|.       :|..:   .||.:.|
  Rat   312 RAEKDPPSMACTRPPSAPRNV-ISNINETSVILDWSWPL-------DTGGR---KDITFNIICKK 365

  Fly   434 ------KCNICSPNVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSL 492
                  :|..|||||.:.|.......|.:|:|:|...|.||.:|.|||.||.::     |.....
  Rat   366 CGWNVRQCEPCSPNVRFLPRQLGLTNTTVTVTDLLAHTNYTFEIDAINGVSELS-----SPPRQF 425

  Fly   493 VAVSDIVFSNTSLLNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQS 557
            .|||                           .||.....|.|..::....:.....|.|.:|...
  Rat   426 AAVS---------------------------ITTNQAAPSPVMTIKKDRTSRNSISLSWQEPEHP 463

  Fly   558 DFPLEFYEVRWFPKVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQ 622
            :..:..|||:::.|.|.:. :.:.|..:.|...|..|..:|.|.||:|.:|..|:|:.|.....:
  Rat   464 NGIILDYEVKYYEKQEQET-SYTILRARGTNVTISSLKPDTTYVFQIRARTAAGYGTNSRKFEFE 527

  Fly   623 TLQSVGSVYDDSVQIRFIA--GAIVTGVLFLVIFIIATVY--FMRSKHQDDLDKKSTNHLPLPLD 683
            |.....|:..::..:..||  .|:...||.:|.:::...:  :.:|||..|
  Rat   528 TSPDSFSISGENSHVVMIAISAAVAIIVLTVVTYVLVGRFCGYHKSKHSSD-------------- 578

  Fly   684 YASNEVNSMDTTPIVKKLHLNVTTPLFGNS-------RSYVDPHTYEDPNQAIREFAREIDANYI 741
                          .|:||       |||.       |:||||||||||.||:.|||:|:||..|
  Rat   579 --------------EKRLH-------FGNGHLRLPGLRTYVDPHTYEDPTQAVHEFAKELDATNI 622

  Fly   742 TIEAIIGGGEFGDVCRGRLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIY 806
            .|:.::|.||||:||.||||:|..  ::|.|||||||.|.:||.|.|||.||||||||||||:|.
  Rat   623 AIDKVVGAGEFGEVCSGRLKLPSK--KEISVAIKTLKVGYTEKQRRDFLGEASIMGQFDHPNIIR 685

  Fly   807 LQGVVTRSNPVMIITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAA 871
            |:||||:|.||||:||||||||||:|||.:|.:|..:||:.|||||||||.|||||.||||||||
  Rat   686 LEGVVTKSKPVMIVTEYMENGSLDSFLRKHDAQFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAA 750

  Fly   872 RNVLVNAQLICKIADFGLSREIENASD-AYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSYGVVL 935
            ||:|:|:.|:||::||||||.:|:..: ||||||||||:|||:|||||:|||||||||||||:||
  Rat   751 RNILINSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTSPEAIAYRKFTSASDVWSYGIVL 815

  Fly   936 WEVMSYGERPYWNWSNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLD 1000
            ||||||||||||..|||||||::::|||||.|||||.||||||||||||.|.:||.|..|||.||
  Rat   816 WEVMSYGERPYWEMSNQDVIKAVDEGYRLPPPMDCPAALYQLMLDCWQKDRNNRPKFEQIVSILD 880

  Fly  1001 NLARQPQSLLTTRPSPESDGNHILDGQRGQNI--FISTDLWLEHIKMSRYCHHFKEANLINAQQI 1063
            .|.|.|.||.....:.....|.:|| |...:|  |.:|..||..::.:.....|......:...|
  Rat   881 KLIRNPGSLKIITSAAARPSNLLLD-QSNVDIATFHTTGDWLNGMRTAHCKEIFTGVEYSSCDTI 944

  Fly  1064 SRLTAQQLSDMGITLVGHQKKILHQARQLDT 1094
            ::::...:..:|:|:||.||||:...:.|:|
  Rat   945 AKISTDDMKKVGVTVVGPQKKIISSIKALET 975

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	68/185 (37%)
		FN3	387..476	CDD:238020	31/98 (32%)
		FN3	536..623	CDD:238020	21/86 (24%)
		EphA2_TM	644..736	CDD:291255	29/100 (29%)
		PTKc_EphR	736..1002	CDD:270629	190/266 (71%)
		Pkinase_Tyr	741..999	CDD:285015	186/258 (72%)
		SAM_1	1033..1092	CDD:278937	14/58 (24%)
		SAM	1039..1092	CDD:197735	12/52 (23%)
Epha3	NP_113752.2	EphR_LBD_A3	29..201	CDD:198449	68/186 (37%)
		fn3	327..417	CDD:394996	32/100 (32%)
		FN3	437..528	CDD:238020	23/91 (25%)
		EphA2_TM	<565..619	CDD:405290	27/88 (31%)
		PTKc_EphR_A	617..883	CDD:270651	190/267 (71%)
		SAM_EPH-A3	913..975	CDD:188943	15/61 (25%)
		PDZ-binding. /evidence=ECO:0000255	982..984
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	399	1.000	Domainoid score	I718
eggNOG	1	0.900	-	-		E2759_KOG0196
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	763	1.000	Inparanoid score	I521
OMA	1	1.010	-	-		QHG45908
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	1	1.000	-	-		FOG0000086
OrthoInspector	1	1.000	-	-		otm46382
orthoMCL	1	0.900	-	-		OOG6_100189
Panther	1	1.100	-	-	O	PTHR24416
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X82
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	12	11.880