DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Eph and Ephb3

DIOPT Version :9

Sequence 1:	NP_524625.3	Gene:	Eph / 43803	FlyBaseID:	FBgn0025936	Length:	1096	Species:	Drosophila melanogaster
Sequence 2:	NP_001099338.1	Gene:	Ephb3 / 287989	RGDID:	1305602	Length:	1048	Species:	Rattus norvegicus

Alignment Length:	1035	Identity:	416/1035 - (40%)
Similarity:	582/1035 - (56%)	Gaps:	116/1035 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly    87 LLDT---TREATLEWTRYPYGPQAQTPGWVEESFTDFVKGIN-WRSYVVCDVAYHNVNNWLWSPF 147
            |:||   |.|  |.||.:|      ..||.|.|..|  :.:| .|:|.||:|...:.||||.:.|
  Rat    34 LMDTKWVTSE--LAWTSHP------ESGWEEVSGYD--EAMNPIRTYQVCNVRESSQNNWLRTGF 88

  Fly   148 IDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDA--ATREPPPWQTDSYRLIARIA 210
            |.|....|:|:|::||:|||:..|....||||||:|.:||.|:  |:...|.|..:.|..:..||
  Rat    89 IWRREVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPFWMENPYVKVDTIA 153

  Fly   211 AGEGRFNQNSDVDINTEVKSIA-VNKKGVYFAFRDQGACISVLAVKVYYITCPAVTENFAHFNET 274
            ..|. |::.....:||:|:|.. ::|.|.|.||:|||||:|:::|:.:|..|.:.|..||.|.||
  Rat   154 PDES-FSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYKKCASTTAGFALFPET 217

  Fly   275 PTGREITIIEKQNGTCVDNAEPYETPTYL-CKGDGKWTILTGGCRCKAGYEPNYTNKTCTECPLG 338
            .||.|.|.:....|||:.||.....|..| |.|||:|.:..|.|.|..|:||......|..||.|
  Rat   218 LTGAEPTSLVIAPGTCIANAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAKETQCRACPPG 282

  Fly   339 TFKSPE-VTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPCYSPPAAPTNLTLLFVDQTS 402
            ::|:.: ...|.||||||..:...:..|.|.:.|||..:|.....|.:.|:.|..: :..|::||
  Rat   283 SYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDTADSACTTVPSPPRGV-ISNVNETS 346

  Fly   403 AIISWSAPAKNESFSSETNSKIYHSDIVYKI---------------KCNICSPNVVYNPSTDTFN 452
            .|:.||.|.....          ..|::|.:               .|:.|..||.:.|......
  Rat   347 LILEWSEPRDLGG----------RDDLLYNVICKKCRGSSGAGGPATCSRCDDNVEFEPRQLGLT 401

  Fly   453 ETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLLNIPLDLNEVKT 517
            |.::.:::|...|.||.::.|:|.|         |.:|.|                       ..
  Rat   402 ERRVHISHLLAHTRYTFEVQAVNGV---------SGKSPL-----------------------PP 434

  Fly   518 GQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRWFPKVELDAINKSAL 582
            ..|.:..||.....|.|..|.:.:.:.....|.|..|.:.:..:..||:::|.|.:..|   |.:
  Rat   435 RYAAVNITTNQAAPSEVPTLHLHSSSGSSLTLSWAPPERPNGVILDYEMKYFEKSKGIA---STV 496

  Fly   583 NTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGS-VYDDSVQIRFIAGAIVT 646
            .:::....:.||..:..|..|||.:|..|:|.||.....:|....|| ......|:..|.|:.|.
  Rat   497 TSQKNSVQLDGLQPDARYVVQVRARTVAGYGQYSRPAEFETTSERGSGAQQLQEQLPLIVGSTVA 561

  Fly   647 GVLFLVIFIIATVYFMRSKHQDDLDKKSTNHLPLPLDYASNEVNSMDTTPIVKKLHLNVTTPLFG 711
            |.:|:|:.::..:..:|.:.|.. |.:.|                       :||...|...:
  Rat   562 GFVFMVVVVVIALVCLRKQRQGP-DAEYT-----------------------EKLQQYVAPRM-- 600

  Fly   712 NSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRGRLKIPPNFVQDIDVAIKT 776
              :.|:||.||||||:|:||||:|||.:.:.||.:||.||||:|||||||:|..  :::.|||||
  Rat   601 --KVYIDPFTYEDPNEAVREFAKEIDVSCVKIEEVIGAGEFGEVCRGRLKLPGR--REVFVAIKT 661

  Fly   777 LKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITEYMENGSLDTFLRVNDGKFQ 841
            ||.|.:|:.|.|||:||||||||||||:|.|:||||:|.||||:||:|||.:||:|||:|||:|.
  Rat   662 LKVGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMILTEFMENCALDSFLRLNDGQFT 726

  Fly   842 TLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFGLSREIEN--ASDAYTTR- 903
            .:||:.||||||:||.|||:||||||||||||:|||:.|:||::||||||.:|:  :...||:.
  Rat   727 VIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDPSDPTYTSSL 791

  Fly   904 GGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWSNQDVIKSIEKGYRLPAPM 968
            |||||:|||||||||:|||||||||||||:|:||||||||||||:.||||||.::|:.||||.||
  Rat   792 GGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVINAVEQDYRLPPPM 856

  Fly   969 DCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTTRPSPESDGNHILDGQ-RGQNI 1032
            |||.||:|||||||.:.|..||.|:.||:|||.|.|...||.....:|......:||.. .....
  Rat   857 DCPTALHQLMLDCWVRDRNLRPKFSQIVNTLDKLIRNAASLKVIASAPSGMSQPLLDRTVPDYTT 921

  Fly  1033 FISTDLWLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDMGITLVGHQKKILHQARQL 1092
            |.:...||:.|||.||...|..|...:...::::||:.|..:|:||.|||||||...:.:
  Rat   922 FTTVGDWLDAIKMGRYKESFVGAGFASFDLVAQMTAEDLLRIGVTLAGHQKKILSSIQDM 981

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	71/179 (40%)
		FN3	387..476	CDD:238020	22/103 (21%)
		FN3	536..623	CDD:238020	21/86 (24%)
		EphA2_TM	644..736	CDD:291255	25/91 (27%)
		PTKc_EphR	736..1002	CDD:270629	182/268 (68%)
		Pkinase_Tyr	741..999	CDD:285015	177/260 (68%)
		SAM_1	1033..1092	CDD:278937	23/58 (40%)
		SAM	1039..1092	CDD:197735	22/52 (42%)
Ephb3	NP_001099338.1	EphR_LBD_B3	31..203	CDD:198446	71/179 (40%)
		fn3	333..431	CDD:278470	24/117 (21%)
		FN3	<468..537	CDD:238020	19/71 (27%)
		EphA2_TM	572..623	CDD:291255	21/78 (27%)
		PTKc_EphR_B	623..891	CDD:173638	182/269 (68%)
		Pkinase_Tyr	628..887	CDD:285015	177/260 (68%)
		SAM_superfamily	917..985	CDD:301707	23/65 (35%)
		SAM	917..984	CDD:197735	23/65 (35%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	399	1.000	Domainoid score	I718
eggNOG	1	0.900	-	-		E2759_KOG0196
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	763	1.000	Inparanoid score	I521
OMA	1	1.010	-	-		QHG45908
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	1	1.000	-	-		FOG0000086
OrthoInspector	1	1.000	-	-		otm46382
orthoMCL	1	0.900	-	-		OOG6_100189
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X82
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	13	12.740