Protein Alignment Eph and Ephb1

DIOPT Version :10

• Sequence 1: NP_524625.3
• Gene: Eph / 43803
• FlyBaseID: FBgn0025936
• Length: 1096
• Species: Drosophila melanogaster
• Sequence 2: NP_001097998.1
• Gene: Ephb1 / 24338
• RGDID: 2556
• Length: 984
• Species: Rattus norvegicus
• Alignment Length: 1075
• Identity: 461/1075 - (42%)
• Similarity: 616/1075 - (57%)
• Gaps: 153/1075 - (14%)

  Fly    65 LRTILLIIISIHFKLAHADQVVLLDT-TREATLEWTRYPYGPQAQTPGWVEESFTDFVKGINW-R 127
            |..:||.:::   ....|.:..|:|| |..|.|.||..|      ..||.|.|..|  :.:|. |
  Rat     3 LDCLLLFLLA---SAVAAMEETLMDTRTATAELGWTANP------ASGWEEVSGYD--ENLNTIR 56

  Fly   128 SYVVCDVAYHNVNNWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDA-- 190
            :|.||:|...|.||||.:.||:|..|:|:|.|::||:||||..|....||||||:|.:||.|:
  Rat    57 TYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVI 121

  Fly   191 ATREPPPWQTDSYRLIARIAAGEGRFNQNSDVD-------INTEVKSIA-VNKKGVYFAFRDQGA 247
            ||::...|....|..:..|||.|. |:|   ||       :||||:|.. :.:.|.|.||:|.||
  Rat   122 ATKKSAFWSEAPYLKVDTIAADES-FSQ---VDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGA 182

  Fly   248 CISVLAVKVYYITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYL-CKGDGKWT 311
            |:|:|:|:|::..||::.:|||.|.||.||.|.|.:....|||:.|||..:.|..| |.|||:|.
  Rat   183 CMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWM 247

  Fly   312 ILTGGCRCKAGYEPNYTNKTCTECPLGTFK-SPEVTKCTPCPPNSNASKTGSPFCKCASGFYRHP 375
            :..|.|.|||||||. .:..|..||.|||| |.|...|:.||.||.:....||.|.|.:|:||..
  Rat   248 VPIGRCTCKAGYEPE-NSVACKACPAGTFKASQEAEGCSHCPSNSRSPSEASPICTCRTGYYRAD 311

  Fly   376 NDGRHMPCYSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKI------- 433
            .|...:.|.|.|:.|.|: :..|::||.|:.|..|       .||..:   .|:.|.|
  Rat   312 FDPPEVACTSVPSGPRNV-ISIVNETSIILEWHPP-------RETGGR---DDVTYNIICKKCRA 365

  Fly   434 ---KCNICSPNVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEF-KRHSNESSLVA 494
               .|:.|..||.:.|......|.::::::|...|.||..|.|||.||..:.| .:|      |:
  Rat   366 DRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPPQH------VS 424

  Fly   495 VSDIVFSNTSLLNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDF 559
            |:                           .||.....|||..:..::.|.:...|.|.:|.|.:.
  Rat   425 VN---------------------------ITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNG 462

  Fly   560 PLEFYEVRWFPKVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTL 624
            .:..||:|::.| |.:..|.|...::...|.|.||.....|..|||.:|..|:|.:|..:..|||
  Rat   463 IILDYEIRYYEK-EHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTL 526

  Fly   625 QSVGSVYDDSV------QIRFIAGAIVTGVLFLVIFIIATV-------YFMRSKHQDDLDKKSTN 676
            .      ||..      |:..|||:...||:|:|..:..::       |...:.:.|.|...||.
  Rat   527 T------DDDYKSELREQLPLIAGSAAAGVVFVVSLVAISIVCSRKRAYSKEAVYSDKLQHYSTG 585

  Fly   677 HLPLPLDYASNEVNSMDTTPIVKKLHLNVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYI 741
            .                .:|.:|               .|:||.||||||:|:||||:|||.:::
  Rat   586 R----------------GSPGMK---------------IYIDPFTYEDPNEAVREFAKEIDVSFV 619

  Fly   742 TIEAIIGGGEFGDVCRGRLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIY 806
            .||.:||.||||:|.:||||:|..  ::|.|||||||.|.|||.|.|||:||||||||||||:|.
  Rat   620 KIEEVIGAGEFGEVYKGRLKLPGK--REIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNIIR 682

  Fly   807 LQGVVTRSNPVMIITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAA 871
            |:||||:|.|||||||:||||:||:|||.|||:|..:||:.||||||:||.|||:||||||||||
  Rat   683 LEGVVTKSRPVMIITEFMENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAA 747

  Fly   872 RNVLVNAQLICKIADFGLSREI-ENASD-AYTTR-GGKIPVRWTAPEAIAFRKFTSASDVWSYGV 933
            ||:|||:.|:||::||||||.: ::.|| .||:. |||||||||||||||:|||||||||||||:
  Rat   748 RNILVNSNLVCKVSDFGLSRYLQDDTSDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVWSYGI 812

  Fly   934 VLWEVMSYGERPYWNWSNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVST 998
            |:|||||:||||||:.||||||.:||:.||||.|||||.||:|||||||||.|..||.||.||:|
  Rat   813 VMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNT 877

  Fly   999 LDNLARQPQSLLTTRPSPESDGNHILDGQRGQNI-----FISTDLWLEHIKMSRYCHHFKEANLI 1058
            ||.:.|.|.||.|...........:||    ::|     |.:.|.||..|||.:|...|..|...
  Rat   878 LDKMIRNPASLKTVATITAVPSQPLLD----RSIPDFTAFTTVDDWLSAIKMVQYRDSFLTAGFT 938

  Fly  1059 NAQQISRLTAQQLSDMGITLVGHQKKIL---HQAR 1090
            :.|.::::|::.|..:|:||.|||||||   |..|
  Rat   939 SLQLVTQMTSEDLLRIGVTLAGHQKKILSSIHSMR 973

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	78/186 (42%)
		FN3	387..476	CDD:238020	27/98 (28%)
		FN3	536..623	CDD:238020	24/86 (28%)
		EphA2_TM	640..738	CDD:464211	30/104 (29%)
		PTKc_EphR	736..1002	CDD:270629	190/268 (71%)
		SAM_1	1029..1092	CDD:425739	26/70 (37%)
Ephb1	NP_001097998.1	EphR_LBD_B1	20..195	CDD:198444	78/186 (42%)
		fn3	326..412	CDD:394996	27/96 (28%)
		fn3	435..518	CDD:394996	26/83 (31%)
		EphA2_TM	542..616	CDD:464211	30/104 (29%)
		PTKc_EphR_B	614..882	CDD:173638	190/269 (71%)
		SAM_EPH-B1	908..975	CDD:188950	25/66 (38%)
		PDZ-binding. /evidence=ECO:0000255	982..984