DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Eph and EPHB3

DIOPT Version :9

Sequence 1:	NP_524625.3	Gene:	Eph / 43803	FlyBaseID:	FBgn0025936	Length:	1096	Species:	Drosophila melanogaster
Sequence 2:	NP_004434.2	Gene:	EPHB3 / 2049	HGNCID:	3394	Length:	998	Species:	Homo sapiens

Alignment Length:	1058	Identity:	421/1058 - (39%)
Similarity:	590/1058 - (55%)	Gaps:	115/1058 - (10%)

- Green bases have known domain annotations that are detailed below.

  Fly    62 MSLLRTILLIIISIHFKLAHADQVVLLDT---TREATLEWTRYPYGPQAQTPGWVEESFTDFVKG 123
            :.||..:||:.:.:......|.:..|:||   |.|  |.||.:|      ..||.|.|..|  :.
Human    17 LPLLPPLLLLPLLLLPAGCRALEETLMDTKWVTSE--LAWTSHP------ESGWEEVSGYD--EA 71

  Fly   124 IN-WRSYVVCDVAYHNVNNWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYE 187
            :| .|:|.||:|...:.||||.:.||.|....|:|:|::||:|||:..|....||||||:|.:||
Human    72 MNPIRTYQVCNVRESSQNNWLRTGFIWRRDVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYE 136

  Fly   188 FDA--ATREPPPWQTDSYRLIARIAAGEGRFNQNSDVDINTEVKSIA-VNKKGVYFAFRDQGACI 249
            .|:  |:...|.|..:.|..:..||..|. |::.....:||:|:|.. ::|.|.|.||:|||||:
Human   137 ADSDVASASSPFWMENPYVKVDTIAPDES-FSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACM 200

  Fly   250 SVLAVKVYYITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYL-CKGDGKWTIL 313
            |:::|:.:|..|.:.|..||.|.||.||.|.|.:....|||:.||.....|..| |.|||:|.:.
Human   201 SLISVRAFYKKCASTTAGFALFPETLTGAEPTSLVIAPGTCIPNAVEVSVPLKLYCNGDGEWMVP 265

  Fly   314 TGGCRCKAGYEPNYTNKTCTECPLGTFKSPE-VTKCTPCPPNSNASKTGSPFCKCASGFYRHPND 377
            .|.|.|..|:||......|..||.|::|:.: ...|.||||||..:...:..|.|.:.|||..:|
Human   266 VGACTCATGHEPAAKESQCRPCPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSD 330

  Fly   378 GRHMPCYSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKI--------- 433
            .....|.:.|:.|..: :..|::||.|:.||.|.....          ..|::|.:
Human   331 SADSACTTVPSPPRGV-ISNVNETSLILEWSEPRDLGG----------RDDLLYNVICKKCHGAG 384

  Fly   434 ---KCNICSPNVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAV 495
               .|:.|..||.:.|......|.::.:::|...|.||.::.|:|.|         |.:|.|
Human   385 GASACSRCDDNVEFVPRQLGLTERRVHISHLLAHTRYTFEVQAVNGV---------SGKSPL--- 437

  Fly   496 SDIVFSNTSLLNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFP 560
                                ....|.:..||.....|.|..||:.:.:.....|.|..|.:.:..
Human   438 --------------------PPRYAAVNITTNQAAPSEVPTLRLHSSSGSSLTLSWAPPERPNGV 482

  Fly   561 LEFYEVRWFPKVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQ 625
            :..||:::|.|.|..|   |.:.::.....:.||..:..|..|||.:|..|:|.||.....:|..
Human   483 ILDYEMKYFEKSEGIA---STVTSQMNSVQLDGLRPDARYVVQVRARTVAGYGQYSRPAEFETTS 544

  Fly   626 SVGS-VYDDSVQIRFIAGAIVTGVLFLV-IFIIATVYFMRSKHQDDLDKKSTNHLPLPLDYASNE 688
            ..|| ......|:..|.|:...|::|:| :.:||.|...:.:|..|
Human   545 ERGSGAQQLQEQLPLIVGSATAGLVFVVAVVVIAIVCLRKQRHGSD------------------- 590

  Fly   689 VNSMDTTPIVKKLHLNVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFG 753
                  :...:||...:...:    :.|:||.||||||:|:||||:|||.:.:.||.:||.||||
Human   591 ------SEYTEKLQQYIAPGM----KVYIDPFTYEDPNEAVREFAKEIDVSCVKIEEVIGAGEFG 645

  Fly   754 DVCRGRLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVM 818
            :|||||||.|..  :::.|||||||.|.:|:.|.|||:||||||||||||:|.|:||||:|.|||
Human   646 EVCRGRLKQPGR--REVFVAIKTLKVGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVM 708

  Fly   819 IITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICK 883
            |:||:|||.:||:|||:|||:|..:||:.||||||:||.|||:||||||||||||:|||:.|:||
Human   709 ILTEFMENCALDSFLRLNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCK 773

  Fly   884 IADFGLSREIEN--ASDAYTTR-GGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERP 945
            ::||||||.:|:  :...||:. |||||:|||||||||:|||||||||||||:|:||||||||||
Human   774 VSDFGLSRFLEDDPSDPTYTSSLGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERP 838

  Fly   946 YWNWSNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLL 1010
            ||:.||||||.::|:.||||.|||||.||:|||||||.:.|..||.|:.||:|||.|.|...||.
Human   839 YWDMSNQDVINAVEQDYRLPPPMDCPTALHQLMLDCWVRDRNLRPKFSQIVNTLDKLIRNAASLK 903

  Fly  1011 TTRPSPESDGNHILDGQ-RGQNIFISTDLWLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDM 1074
            ....:.......:||.. .....|.:...||:.|||.||...|..|...:...::::||:.|..:
Human   904 VIASAQSGMSQPLLDRTVPDYTTFTTVGDWLDAIKMGRYKESFVSAGFASFDLVAQMTAEDLLRI 968

  Fly  1075 GITLVGHQKKILHQARQL 1092
            |:||.|||||||...:.:
Human   969 GVTLAGHQKKILSSIQDM 986

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	71/181 (39%)
		FN3	387..476	CDD:238020	22/100 (22%)
		FN3	536..623	CDD:238020	23/86 (27%)
		EphA2_TM	644..736	CDD:291255	24/92 (26%)
		PTKc_EphR	736..1002	CDD:270629	182/268 (68%)
		Pkinase_Tyr	741..999	CDD:285015	177/260 (68%)
		SAM_1	1033..1092	CDD:278937	23/58 (40%)
		SAM	1039..1092	CDD:197735	22/52 (42%)
EPHB3	NP_004434.2	EphR_LBD_B3	39..211	CDD:198446	71/182 (39%)
		Ephrin_rec_like	<284..316	CDD:311571	12/31 (39%)
		fn3	341..436	CDD:365830	24/114 (21%)
		FN3	<473..542	CDD:238020	20/71 (28%)
		EphA2_TM	<581..628	CDD:373140	18/75 (24%)
		PTKc_EphR_B	628..896	CDD:173638	182/269 (68%)
		SAM_superfamily	922..990	CDD:387673	23/65 (35%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	399	1.000	Domainoid score	I741
eggNOG	1	0.900	-	-		E2759_KOG0196
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	762	1.000	Inparanoid score	I548
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG45908
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	1	1.000	-	-		FOG0000086
OrthoInspector	1	1.000	-	-		otm42229
orthoMCL	1	0.900	-	-		OOG6_100189
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X82
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	12	11.740