DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Eph and EPHA8

DIOPT Version :9

Sequence 1:	NP_524625.3	Gene:	Eph / 43803	FlyBaseID:	FBgn0025936	Length:	1096	Species:	Drosophila melanogaster
Sequence 2:	NP_065387.1	Gene:	EPHA8 / 2046	HGNCID:	3391	Length:	1005	Species:	Homo sapiens

Alignment Length:	1048	Identity:	417/1048 - (39%)
Similarity:	575/1048 - (54%)	Gaps:	138/1048 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly    84 QVVLLDT-TREATLEWTRYPYGPQAQTPGW-----VEESFTDFVKGINWRSYVVCDVAYHNVNNW 142
            :|.|||| |......|..||      ..||     |:|||...      .:|.||:|...|.|||
Human    31 EVNLLDTSTIHGDWGWLTYP------AHGWDSINEVDESFQPI------HTYQVCNVMSPNQNNW 83

  Fly   143 LWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFD----AATREPPPWQTDSY 203
            |.:.::.|..|.|:|.||:||:|||:..||...:|||||:|.:.|.|    |:|:|      ..:
Human    84 LRTSWVPRDGARRVYAEIKFTLRDCNSMPGVLGTCKETFNLYYLESDRDLGASTQE------SQF 142

  Fly   204 RLIARIAAGEGRFNQNSDV---DINTEVKSIA-VNKKGVYFAFRDQGACISVLAVKVYYITCPAV 264
            ..|..|||.|.....:..|   .:||||:|:. ::|:|.|.||:|.|||:::|::::||..|||:
Human   143 LKIDTIAADESFTGADLGVRRLKLNTEVRSVGPLSKRGFYLAFQDIGACLAILSLRIYYKKCPAM 207

  Fly   265 TENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYLCKGDGKWTILTGGCRCKAGYEPNYTN 329
            ..|.|.|:|..||.:.:.:.:..|.||.::|..:||...|..:|:|.:..|.|.|.||||..  .
Human   208 VRNLAAFSEAVTGADSSSLVEVRGQCVRHSEERDTPKMYCSAEGEWLVPIGKCVCSAGYEER--R 270

  Fly   330 KTCTECPLGTFKS-PEVTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPCYSPPAAPTNL 393
            ..|..|.||.:|| |....|..|||:|:::...:..|.|...:||...|.....|..||:||.||
Human   271 DACVACELGFYKSAPGDQLCARCPPHSHSAAPAAQACHCDLSYYRAALDPPSSACTRPPSAPVNL 335

  Fly   394 TLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKI----------KCNICSPNVVYNPST 448
             :..|:.||..:.|:.|.....          .|||.|..          :|..|.....:.|..
Human   336 -ISSVNGTSVTLEWAPPLDPGG----------RSDITYNAVCRRCPWALSRCEACGSGTRFVPQQ 389

  Fly   449 DTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLLNIPLDLN 513
            .:..:..:.:.||.....|:..|.|:|.||.::...|.:                :::||
Human   390 TSLVQASLLVANLLAHMNYSFWIEAVNGVSDLSPEPRRA----------------AVVNI----- 433

  Fly   514 EVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRWFPKVELDAIN 578
                       ||.....|.|..:|..........|.|.:|.|.:..:..||::::.| :.:..:
Human   434 -----------TTNQAAPSQVVVIRQERAGQTSVSLLWQEPEQPNGIILEYEIKYYEK-DKEMQS 486

  Fly   579 KSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGSV---YDDSVQIRFI 640
            .|.|....|:|.:.||...|.|.||||.:|:.|.|.:|..:..:|    |..   ||... |.:|
Human   487 YSTLKAVTTRATVSGLKPGTRYVFQVRARTSAGCGRFSQAMEVET----GKPRPRYDTRT-IVWI 546

  Fly   641 AGAIVTGVLFLVIFIIATVYFMRSKH-------QDDLDKKSTNHLPLPLDYASNEVNSMDTTPIV 698
            ...::||::.|::.:|.     :.:|       ||..::|        :.|.    |.....|:.
Human   547 CLTLITGLVVLLLLLIC-----KKRHCGYSKAFQDSDEEK--------MHYQ----NGQAPPPVF 594

  Fly   699 KKLHL---NVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRGRL 760
            ..||.   .:..|.|     |.:|||||:|.:|.|.|.|||:|:.|.||.|||.|:.|:||.|||
Human   595 LPLHHPPGKLPEPQF-----YAEPHTYEEPGRAGRSFTREIEASRIHIEKIIGSGDSGEVCYGRL 654

  Fly   761 KIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITEYME 825
            ::|..  :|:.||||.||.|.:|:.|.|||:||||||||||||:|.|:|||||....||:|||||
Human   655 RVPGQ--RDVPVAIKALKAGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTRGRLAMIVTEYME 717

  Fly   826 NGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFGLS 890
            |||||||||.:||:|..:||:.||||:.:||.||||:.|||||||||||||::.|:||::|||||
Human   718 NGSLDTFLRTHDGQFTIMQLVGMLRGVGAGMRYLSDLGYVHRDLAARNVLVDSNLVCKVSDFGLS 782

  Fly   891 REIENASD-AYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWSNQDV 954
            |.:|:..| ||||.|||||:|||||||||||.|:|||||||:|||:|||::||||||||.:|:||
Human   783 RVLEDDPDAAYTTTGGKIPIRWTAPEAIAFRTFSSASDVWSFGVVMWEVLAYGERPYWNMTNRDV 847

  Fly   955 IKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTT----RPS 1015
            |.|:|:||||||||.||.||:|||||||.|.|..||.|:.|||.||.|.|.|:||..|    |..
Human   848 ISSVEEGYRLPAPMGCPHALHQLMLDCWHKDRAQRPRFSQIVSVLDALIRSPESLRATATVSRCP 912

  Fly  1016 PESDGNHILD--GQRGQNIFISTDLWLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDMGITL 1078
            |.:......|  |..|....::...||:.|:|.||..||......:...:.|:.||.:..:||||
Human   913 PPAFVRSCFDLRGGSGGGGGLTVGDWLDSIRMGRYRDHFAAGGYSSLGMVLRMNAQDVRALGITL 977

  Fly  1079 VGHQKKIL 1086
            :|||||||
Human   978 MGHQKKIL 985

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	71/188 (38%)
		FN3	387..476	CDD:238020	22/98 (22%)
		FN3	536..623	CDD:238020	24/86 (28%)
		EphA2_TM	644..736	CDD:291255	26/101 (26%)
		PTKc_EphR	736..1002	CDD:270629	180/266 (68%)
		Pkinase_Tyr	741..999	CDD:285015	176/258 (68%)
		SAM_1	1033..1092	CDD:278937	22/54 (41%)
		SAM	1039..1092	CDD:197735	22/48 (46%)
EPHA8	NP_065387.1	EphR_LBD	31..203	CDD:295439	71/189 (38%)
		fn3	330..423	CDD:278470	24/103 (23%)
		FN3	440..531	CDD:238020	26/91 (29%)
		Mediates interaction with ANKS1A and ANKS1B. /evidence=ECO:0000250	564..570		1/5 (20%)
		EphA2_TM	<566..630	CDD:291255	22/80 (28%)
		Mediates interaction with PIK3CG and required for endocytosis. /evidence=ECO:0000250	589..644		25/59 (42%)
		PTKc_EphR_A	630..896	CDD:270651	180/267 (67%)
		Pkinase_Tyr	635..892	CDD:285015	176/258 (68%)
		SAM_EPH-A8	933..995	CDD:188949	22/53 (42%)
		SAM	934..994	CDD:197735	22/52 (42%)
		PDZ-binding. /evidence=ECO:0000255	1003..1005
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	399	1.000	Domainoid score	I741
eggNOG	1	0.900	-	-		E2759_KOG0196
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	762	1.000	Inparanoid score	I548
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG45908
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	1	1.000	-	-		FOG0000086
OrthoInspector	1	1.000	-	-		otm42229
orthoMCL	1	0.900	-	-		OOG6_100189
Panther	1	1.100	-	-	O	PTHR24416
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X82
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	12	11.880