DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Eph and Epha8

DIOPT Version :9

Sequence 1:	NP_524625.3	Gene:	Eph / 43803	FlyBaseID:	FBgn0025936	Length:	1096	Species:	Drosophila melanogaster
Sequence 2:	NP_031965.2	Gene:	Epha8 / 13842	MGIID:	109378	Length:	1004	Species:	Mus musculus

Alignment Length:	1045	Identity:	412/1045 - (39%)
Similarity:	574/1045 - (54%)	Gaps:	132/1045 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly    84 QVVLLDT-TREATLEWTRYPYGPQAQTPGW-----VEESFTDFVKGINWRSYVVCDVAYHNVNNW 142
            :|.|||| |......|..||      ..||     |:|||...      .:|.||:|...|.|||
Mouse    30 EVNLLDTSTIHGDWGWLTYP------AHGWDSINEVDESFRPI------HTYQVCNVMSPNQNNW 82

  Fly   143 LWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFD----AATREPPPWQTDSY 203
            |.:.::.|..|.|:|.||:||:|||:..||...:|||||:|.:.|.|    |:|:|      ..:
Mouse    83 LRTNWVPRDGARRVYAEIKFTLRDCNSIPGVLGTCKETFNLHYLESDRDLGASTQE------SQF 141

  Fly   204 RLIARIAAGEGRFNQNSDV---DINTEVKSIA-VNKKGVYFAFRDQGACISVLAVKVYYITCPAV 264
            ..|..|||.|.....:..|   .:||||:.:. ::|:|.|.||:|.|||:::|::::||..|||:
Mouse   142 LKIDTIAADESFTGADLGVRRLKLNTEVRGVGPLSKRGFYLAFQDIGACLAILSLRIYYKKCPAM 206

  Fly   265 TENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYLCKGDGKWTILTGGCRCKAGYEPNYTN 329
            ..|.|.|:|..||.:.:.:.:..|.||.::|..:||...|..:|:|.:..|.|.|.||||..  .
Mouse   207 VRNLAAFSEAVTGADSSSLVEVRGQCVRHSEERDTPKMYCSAEGEWLVPIGKCVCSAGYEER--R 269

  Fly   330 KTCTECPLGTFKS-PEVTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPCYSPPAAPTNL 393
            ..|..|.||.:|| |....|..|||:|:::...:..|:|...:||...|.....|..||:||.||
Mouse   270 DACMACELGFYKSAPGDQLCARCPPHSHSATPAAQTCRCDLSYYRAALDPPSAACTRPPSAPVNL 334

  Fly   394 TLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKI----------KCNICSPNVVYNPST 448
             :..|:.||..:.|:.|.....          .|||.|..          .|..|.....:.|..
Mouse   335 -ISSVNGTSVTLEWAPPLDPGG----------RSDITYNAVCRRCPWALSHCEACGSGTRFVPQQ 388

  Fly   449 DTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLLNIPLDLN 513
            .:..:..:.:.||.....|:..|.|:|.||:::...|                :.:::||
Mouse   389 TSLAQASLLVANLLAHMNYSFWIEAVNGVSNLSPEPR----------------SAAVVNI----- 432

  Fly   514 EVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRWFPKVELDAIN 578
                       ||.....|.|..:|..........|.|.:|.|.:..:..||::::.| :.:..:
Mouse   433 -----------TTNQAAPSQVVVIRQERAGQTSVSLLWQEPEQPNGIILEYEIKYYEK-DKEMQS 485

  Fly   579 KSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGSV---YDDSVQIRFI 640
            .|.|....|:|.:.||...|.|.||||.:|:.|.|.:|..:..:|    |..   ||... |.:|
Mouse   486 YSTLKAVTTRATVSGLKPGTRYVFQVRARTSAGCGRFSQAMEVET----GKPRPRYDTRT-IVWI 545

  Fly   641 AGAIVTGVLFLVIFIIATVYFMRSKH-------QDDLDKKSTNHLPLPLDYASNEVNSMDTTPIV 698
            ...::||::.|::.:|.     :.:|       ||..::|        :.|.    |.....|:.
Mouse   546 CLTLITGLVVLLLLLIC-----KKRHCGYSKAFQDSDEEK--------MHYQ----NGQAPPPVF 593

  Fly   699 KKLHLNVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRGRLKIP 763
              |.||.....|..::...:|||||:|.:|.|.|.|||:|:.|.||.|||.||.|:||.|||::|
Mouse   594 --LPLNHPPGKFPETQFSAEPHTYEEPGRAGRSFTREIEASRIHIEKIIGSGESGEVCYGRLQVP 656

  Fly   764 PNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITEYMENGS 828
            ..  :|:.||||.||.|.:|:.|.|||:||:|||||||||:|.|:|||||....||:||||||||
Mouse   657 GQ--RDVPVAIKALKAGYTERQRQDFLSEAAIMGQFDHPNIIRLEGVVTRGRLAMIVTEYMENGS 719

  Fly   829 LDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFGLSREI 893
            ||.|||.:||:|..:||:.||||:.:||.||||:.|:|||||||||||:.:|:||::||||||.:
Mouse   720 LDAFLRTHDGQFTIVQLVGMLRGVGAGMRYLSDLGYIHRDLAARNVLVDGRLVCKVSDFGLSRAL 784

  Fly   894 ENASD-AYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWSNQDVIKS 957
            |:..: ||||.|||||:|||||||||||.|:|||||||:|||:|||::||||||||.:|||||.|
Mouse   785 EDDPEAAYTTAGGKIPIRWTAPEAIAFRTFSSASDVWSFGVVMWEVLAYGERPYWNMTNQDVISS 849

  Fly   958 IEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTT----RPSPES 1018
            :|:||||||||.||.||:|||||||.|.|..||.||.:||.||.|...|:||..|    |..|.:
Mouse   850 VEEGYRLPAPMGCPRALHQLMLDCWHKDRAQRPRFAHVVSVLDALVHSPESLRATATVSRCPPPA 914

  Fly  1019 DGNHILDGQRG--QNIFISTDLWLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDMGITLVGH 1081
            ......|.:.|  .|..::...||:.|:|.||..||......:...:.|:.||.:..:||||:||
Mouse   915 FARSCFDLRAGGSGNGDLTVGDWLDSIRMGRYRDHFAAGGYSSLGMVLRMNAQDVRALGITLMGH 979

  Fly  1082 QKKIL 1086
            |||||
Mouse   980 QKKIL 984

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	70/188 (37%)
		FN3	387..476	CDD:238020	22/98 (22%)
		FN3	536..623	CDD:238020	24/86 (28%)
		EphA2_TM	644..736	CDD:291255	25/98 (26%)
		PTKc_EphR	736..1002	CDD:270629	178/266 (67%)
		Pkinase_Tyr	741..999	CDD:285015	174/258 (67%)
		SAM_1	1033..1092	CDD:278937	22/54 (41%)
		SAM	1039..1092	CDD:197735	22/48 (46%)
Epha8	NP_031965.2	EphR_LBD_A8	30..202	CDD:198454	70/189 (37%)
		fn3	329..422	CDD:278470	24/103 (23%)
		FN3	439..530	CDD:238020	26/91 (29%)
		Mediates interaction with ANKS1A and ANKS1B. /evidence=ECO:0000269\|PubMed:17875921	563..569		1/5 (20%)
		EphA2_TM	<565..629	CDD:291255	21/77 (27%)
		Mediates interaction with PIK3CG and required for endocytosis. /evidence=ECO:0000269\|PubMed:11416136	588..643		24/56 (43%)
		PKc_like	629..895	CDD:304357	178/267 (67%)
		Pkinase_Tyr	634..891	CDD:285015	174/258 (67%)
		SAM_EPH-A8	932..994	CDD:188949	22/53 (42%)
		SAM	933..993	CDD:197735	22/52 (42%)
		PDZ-binding. /evidence=ECO:0000255	1002..1004
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	398	1.000	Domainoid score	I745
eggNOG	1	0.900	-	-		E2759_KOG0196
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	760	1.000	Inparanoid score	I548
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG45908
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	1	1.000	-	-		FOG0000086
OrthoInspector	1	1.000	-	-		otm44283
orthoMCL	1	0.900	-	-		OOG6_100189
Panther	1	1.100	-	-	O	PTHR24416
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X82
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	12	11.880