DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Eph and Epha3

DIOPT Version :9

Sequence 1:	NP_524625.3	Gene:	Eph / 43803	FlyBaseID:	FBgn0025936	Length:	1096	Species:	Drosophila melanogaster
Sequence 2:	NP_034270.1	Gene:	Epha3 / 13837	MGIID:	99612	Length:	984	Species:	Mus musculus

Alignment Length:	1075	Identity:	425/1075 - (39%)
Similarity:	607/1075 - (56%)	Gaps:	146/1075 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly    62 MSLLRTILLIIISIHFKLA--HADQVVLLDT-TREATLEWTRYPYGPQAQTPGW-----VEESFT 118
            :|:|..:...::|...:|:  .:::|.|||: |.:..|.|..||      :.||     |:|.:|
Mouse     5 LSILVLLGCCVLSCSGELSPQPSNEVNLLDSKTIQGELGWISYP------SHGWEEISGVDEHYT 63

  Fly   119 DFVKGINWRSYVVCDVAYHNVNNWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSL 183
            ..      |:|.||:|..|:.||||.:.::.|.||.::|:|::||:|||:..|....:|||||:|
Mouse    64 PI------RTYQVCNVMDHSQNNWLRTNWVPRNSAQKIYVELKFTLRDCNSIPLVLGTCKETFNL 122

  Fly   184 LFYEFD----AATREPPPWQTDSYRLIARIAAGEGRFNQ----NSDVDINTEVKSIA-VNKKGVY 239
            .:.|.|    ...||      ..:..|..|||.|. |.|    :..:.:|||::.:. |||||.|
Mouse   123 YYMESDDDHGVKFRE------HQFTKIDTIAADES-FTQMDLGDRILKLNTEIREVGPVNKKGFY 180

  Fly   240 FAFRDQGACISVLAVKVYYITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYLC 304
            .||:|.|||:::::|:||:..||...:|.|.|.:|......:::|.: |:||:|::..:.|...|
Mouse   181 LAFQDVGACVALVSVRVYFKKCPFTVKNLAMFPDTVPMDSQSLVEVR-GSCVNNSKEEDPPRMYC 244

  Fly   305 KGDGKWTILTGGCRCKAGYEPNYTNKTCTECPLGTFKSPE-VTKCTPCPPNSNASKTGSPFCKCA 368
            ..:|:|.:..|.|.|.||||..  ...|..|..|.:|:.: ..||..|||:|:..:.||..|:|.
Mouse   245 STEGEWLVPIGKCTCNAGYEER--GFICQACRPGFYKASDGAAKCAKCPPHSSTQEDGSMNCRCE 307

  Fly   369 SGFYRHPNDGRHMPCYSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKI 433
            :.::|...|...|.|..||:||.|: :..:::||.|:.||.|.       :|..:   .||.:.|
Mouse   308 NNYFRAEKDPPSMACTRPPSAPRNV-ISNINETSVILDWSWPL-------DTGGR---KDITFNI 361

  Fly   434 ----------KCNICSPNVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSN 488
                      :|..|||||.:.|.......|.:|:|:|...|.||.:|.|:|.||.::...|.
Mouse   362 ICKKCGWNVRQCEPCSPNVRFLPRQLGLTNTTVTVTDLLAHTNYTFEIDAVNGVSELSSPPRQ-- 424

  Fly   489 ESSLVAVSDIVFSNTSLLNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDK 553
                                          .|.:..||.....|.|..::....:.....|.|.:
Mouse   425 ------------------------------YAAVSITTNQAAPSPVMTIKKDRTSRNSISLSWQE 459

  Fly   554 PVQSDFPLEFYEVRWFPKVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNM 618
            |...:..:..|||:::.|.|.:. :.:.|..:.|...|..|..:|.|.||:|.:|..|:|:.|..
Mouse   460 PEHPNGIILDYEVKYYEKQEQET-SYTILRARGTNVTISSLKPDTTYVFQIRARTAAGYGTNSRK 523

  Fly   619 IYAQTLQSVGSVYDDSVQIRFIA--GAIVTGVLFLVIFIIATVY--FMRSKHQDDLDKKSTNHLP 679
            ...:|.....|:..::..:..||  .|:...||.:|.:::...:  :.:|||..:
Mouse   524 FEFETSPDSFSISGENSHVVMIAISAAVAIIVLTVVTYVLVGRFCGYHKSKHSAE---------- 578

  Fly   680 LPLDYASNEVNSMDTTPIVKKLHLNVTTPLFGNS-------RSYVDPHTYEDPNQAIREFAREID 737
                              .|:||       |||.       |:||||||||||.||:.|||:|:|
Mouse   579 ------------------EKRLH-------FGNGHLKLPGLRTYVDPHTYEDPTQAVHEFAKELD 618

  Fly   738 ANYITIEAIIGGGEFGDVCRGRLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHP 802
            |..|:|:.::|.||||:||.||||:|..  ::|.|||||||.|.:||.|.|||.|||||||||||
Mouse   619 ATNISIDKVVGAGEFGEVCSGRLKLPSK--KEISVAIKTLKVGYTEKQRRDFLGEASIMGQFDHP 681

  Fly   803 NVIYLQGVVTRSNPVMIITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHR 867
            |:|.|:||||:|.||||:||||||||||:|||.:|.:|..:||:.|||||||||.|||||.||||
Mouse   682 NIIRLEGVVTKSKPVMIVTEYMENGSLDSFLRKHDAQFTVIQLVGMLRGIASGMKYLSDMGYVHR 746

  Fly   868 DLAARNVLVNAQLICKIADFGLSREIENASD-AYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSY 931
            ||||||:|:|:.|:||::||||||.:|:..: ||||||||||:|||:|||||:||||||||||||
Mouse   747 DLAARNILINSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTSPEAIAYRKFTSASDVWSY 811

  Fly   932 GVVLWEVMSYGERPYWNWSNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIV 996
            |:||||||||||||||..|||||||::::|||||.|||||.||||||||||||.|.:||.|..||
Mouse   812 GIVLWEVMSYGERPYWEMSNQDVIKAVDEGYRLPPPMDCPAALYQLMLDCWQKDRNNRPKFEQIV 876

  Fly   997 STLDNLARQPQSLLTTRPSPESDGNHILDGQRGQNI--FISTDLWLEHIKMSRYCHHFKEANLIN 1059
            |.||.|.|.|.||.....:.....|.:|| |...:|  |.:|..||..::.:.....|......:
Mouse   877 SILDKLIRNPGSLKIITSAAARPSNLLLD-QSNVDIATFHTTGDWLNGMRTAHCKEIFTGVEYSS 940

  Fly  1060 AQQISRLTAQQLSDMGITLVGHQKKILHQARQLDT 1094
            ...|::::...:..:|:|:||.||||:...:.|:|
Mouse   941 CDTIAKISTDDMKKVGVTVVGPQKKIISTIKALET 975

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	70/189 (37%)
		FN3	387..476	CDD:238020	30/98 (31%)
		FN3	536..623	CDD:238020	21/86 (24%)
		EphA2_TM	644..736	CDD:291255	28/100 (28%)
		PTKc_EphR	736..1002	CDD:270629	190/266 (71%)
		Pkinase_Tyr	741..999	CDD:285015	186/258 (72%)
		SAM_1	1033..1092	CDD:278937	14/58 (24%)
		SAM	1039..1092	CDD:197735	12/52 (23%)
Epha3	NP_034270.1	EphR_LBD_A3	29..201	CDD:198449	70/190 (37%)
		fn3	327..417	CDD:278470	31/100 (31%)
		FN3	437..528	CDD:238020	23/91 (25%)
		EphA2_TM	<566..617	CDD:291255	25/85 (29%)
		PTKc_EphR_A	617..883	CDD:270651	190/267 (71%)
		Pkinase_Tyr	622..879	CDD:285015	186/258 (72%)
		SAM	909..976	CDD:197735	17/67 (25%)
		SAM_EPH-A3	913..975	CDD:188943	15/61 (25%)
		PDZ-binding. /evidence=ECO:0000255	982..984
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	398	1.000	Domainoid score	I745
eggNOG	1	0.900	-	-		E2759_KOG0196
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	760	1.000	Inparanoid score	I548
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG45908
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	1	1.000	-	-		FOG0000086
OrthoInspector	1	1.000	-	-		otm44283
orthoMCL	1	0.900	-	-		OOG6_100189
Panther	1	1.100	-	-	O	PTHR24416
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X82
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	13	12.880